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Protein

Transcription elongation factor A protein 3

Gene

TCEA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri306 – 34641TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 3
Alternative name(s):
Transcription elongation factor S-II protein 3
Transcription elongation factor TFIIS.h
Gene namesi
Name:TCEA3
Synonyms:TFIISH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:11615. TCEA3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36374.

Polymorphism and mutation databases

BioMutaiTCEA3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Transcription elongation factor A protein 3PRO_0000121452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei115 – 1151PhosphoserineCombined sources
Modified residuei140 – 1401PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75764.
MaxQBiO75764.
PaxDbiO75764.
PeptideAtlasiO75764.
PRIDEiO75764.

PTM databases

iPTMnetiO75764.
PhosphoSiteiO75764.

Expressioni

Gene expression databases

BgeeiO75764.
CleanExiHS_TCEA3.
ExpressionAtlasiO75764. baseline and differential.
GenevisibleiO75764. HS.

Organism-specific databases

HPAiHPA044960.
HPA055811.

Interactioni

Protein-protein interaction databases

BioGridi112782. 1 interaction.
IntActiO75764. 1 interaction.
STRINGi9606.ENSP00000406293.

Structurei

3D structure databases

ProteinModelPortaliO75764.
SMRiO75764. Positions 4-89, 183-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8278TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini187 – 303117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi130 – 17344Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri306 – 34641TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiO75764.
OMAiFAPSMCL.
OrthoDBiEOG7GQXWQ.
PhylomeDBiO75764.
TreeFamiTF314970.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75764-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQEEELLRI AKKLEKMVAR KNTEGALDLL KKLHSCQMSI QLLQTTRIGV
60 70 80 90 100
AVNGVRKHCS DKEVVSLAKV LIKNWKRLLD SPGPPKGEKG EEREKAKKKE
110 120 130 140 150
KGLECSDWKP EAGLSPPRKK REDPKTRRDS VDSKSSASSS PKRPSVERSN
160 170 180 190 200
SSKSKAESPK TPSSPLTPTF ASSMCLLAPC YLTGDSVRDK CVEMLSAALK
210 220 230 240 250
ADDDYKDYGV NCDKMASEIE DHIYQELKST DMKYRNRVRS RISNLKDPRN
260 270 280 290 300
PGLRRNVLSG AISAGLIAKM TAEEMASDEL RELRNAMTQE AIREHQMAKT
310 320 330 340
GGTTTDLFQC SKCKKKNCTY NQVQTRSADE PMTTFVLCNE CGNRWKFC
Length:348
Mass (Da):38,972
Last modified:February 12, 2003 - v2
Checksum:i3D0A046B5096753D
GO
Isoform 2 (identifier: O75764-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-154: SNSSKS → ACSLMQ
     155-348: Missing.

Note: No experimental confirmation available.
Show »
Length:154
Mass (Da):17,258
Checksum:i0906A294B41EEFBF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei149 – 1546SNSSKS → ACSLMQ in isoform 2. 1 PublicationVSP_056838
Alternative sequencei155 – 348194Missing in isoform 2. 1 PublicationVSP_056839Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY540752 mRNA. Translation: AAT44402.1.
AK290272 mRNA. Translation: BAF82961.1.
AL109936, AL357134 Genomic DNA. Translation: CAI19025.1.
AL357134, AL109936 Genomic DNA. Translation: CAI22178.1.
BC041613 mRNA. Translation: AAH41613.1.
AJ223473 mRNA. Translation: CAA11393.1.
CCDSiCCDS44086.1. [O75764-1]
RefSeqiNP_003187.1. NM_003196.2. [O75764-1]
UniGeneiHs.446354.

Genome annotation databases

EnsembliENST00000374601; ENSP00000363729; ENSG00000204219. [O75764-2]
ENST00000450454; ENSP00000406293; ENSG00000204219. [O75764-1]
ENST00000634832; ENSP00000488968; ENSG00000282941. [O75764-2]
ENST00000635035; ENSP00000489592; ENSG00000282941. [O75764-1]
GeneIDi6920.
KEGGihsa:6920.
UCSCiuc057dfl.1. human. [O75764-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY540752 mRNA. Translation: AAT44402.1.
AK290272 mRNA. Translation: BAF82961.1.
AL109936, AL357134 Genomic DNA. Translation: CAI19025.1.
AL357134, AL109936 Genomic DNA. Translation: CAI22178.1.
BC041613 mRNA. Translation: AAH41613.1.
AJ223473 mRNA. Translation: CAA11393.1.
CCDSiCCDS44086.1. [O75764-1]
RefSeqiNP_003187.1. NM_003196.2. [O75764-1]
UniGeneiHs.446354.

3D structure databases

ProteinModelPortaliO75764.
SMRiO75764. Positions 4-89, 183-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112782. 1 interaction.
IntActiO75764. 1 interaction.
STRINGi9606.ENSP00000406293.

PTM databases

iPTMnetiO75764.
PhosphoSiteiO75764.

Polymorphism and mutation databases

BioMutaiTCEA3.

Proteomic databases

EPDiO75764.
MaxQBiO75764.
PaxDbiO75764.
PeptideAtlasiO75764.
PRIDEiO75764.

Protocols and materials databases

DNASUi6920.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374601; ENSP00000363729; ENSG00000204219. [O75764-2]
ENST00000450454; ENSP00000406293; ENSG00000204219. [O75764-1]
ENST00000634832; ENSP00000488968; ENSG00000282941. [O75764-2]
ENST00000635035; ENSP00000489592; ENSG00000282941. [O75764-1]
GeneIDi6920.
KEGGihsa:6920.
UCSCiuc057dfl.1. human. [O75764-1]

Organism-specific databases

CTDi6920.
GeneCardsiTCEA3.
HGNCiHGNC:11615. TCEA3.
HPAiHPA044960.
HPA055811.
MIMi604128. gene.
neXtProtiNX_O75764.
PharmGKBiPA36374.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiO75764.
OMAiFAPSMCL.
OrthoDBiEOG7GQXWQ.
PhylomeDBiO75764.
TreeFamiTF314970.

Miscellaneous databases

ChiTaRSiTCEA3. human.
GenomeRNAii6920.
PROiO75764.
SOURCEiSearch...

Gene expression databases

BgeeiO75764.
CleanExiHS_TCEA3.
ExpressionAtlasiO75764. baseline and differential.
GenevisibleiO75764. HS.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SEREX-defined rhabdomyosarcoma antigens."
    Behrends U., Gotz C., Mautner J.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Embryonic rhabdomyosarcoma.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.
  5. "Identification of novel genes encoding transcription elongation factor TFIIS (TCEA) in vertebrates: conservation of three distinct TFIIS isoforms in frog, mouse, and human."
    Labhart P., Morgan G.T.
    Genomics 52:278-288(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-348 (ISOFORM 1).
    Tissue: Liver and Spleen.
  6. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115 AND SER-140, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTCEA3_HUMAN
AccessioniPrimary (citable) accession number: O75764
Secondary accession number(s): A8K2K7, Q5DR83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: July 6, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.