O75762 (TRPA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transient receptor potential cation channel subfamily A member 1

Alternative name(s):
Ankyrin-like with transmembrane domains protein 1
Transformation-sensitive protein p120

Gene names

Name:	TRPA1
Synonyms:	ANKTM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1119 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system By similarity.
Enzyme regulation	Inhibited by ruthenium red, a potent blocker of TRPV channels By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells. Ref.1
Post-translational modification	TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain By similarity.
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. [View classification] Contains 15 ANK repeats.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat Transmembrane Transmembrane helix
Molecular function	Ion channel
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of chemical stimulus involved in sensory perception of pain Inferred from electronic annotation. Source: Compara detection of mechanical stimulus involved in sensory perception of pain Inferred from electronic annotation. Source: Compara ion transport Traceable author statement Ref.1. Source: ProtInc response to cold Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from electronic annotation. Source: Compara response to pain Inferred from electronic annotation. Source: Compara thermoception Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc stereocilium bundle Inferred from electronic annotation. Source: Compara
Molecular_function	calcium channel activity Inferred from electronic annotation. Source: Compara channel activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1119	1119	Transient receptor potential cation channel subfamily A member 1		PRO_0000215369
Regions
	Topological domain	1 – 719	719	Cytoplasmic Potential
	Transmembrane	720 – 740	21	Helical; Name=1; Potential
	Topological domain	741 – 772	32	Extracellular Potential
	Transmembrane	773 – 793	21	Helical; Name=2; Potential
	Topological domain	794 – 805	12	Cytoplasmic Potential
	Transmembrane	806 – 826	21	Helical; Name=3; Potential
	Topological domain	827 – 829	3	Extracellular Potential
	Transmembrane	830 – 850	21	Helical; Name=4; Potential
	Topological domain	851 – 873	23	Cytoplasmic Potential
	Transmembrane	874 – 894	21	Helical; Name=5; Potential
	Topological domain	895 – 940	46	Extracellular Potential
	Transmembrane	941 – 961	21	Helical; Name=6; Potential
	Topological domain	962 – 1119	158	Cytoplasmic Potential
	Repeat	62 – 92	31	ANK 1
	Repeat	97 – 126	30	ANK 2
	Repeat	130 – 160	31	ANK 3
	Repeat	164 – 193	30	ANK 4
	Repeat	197 – 226	30	ANK 5
	Repeat	238 – 267	30	ANK 6
	Repeat	271 – 301	31	ANK 7
	Repeat	308 – 337	30	ANK 8
	Repeat	341 – 370	30	ANK 9
	Repeat	412 – 441	30	ANK 10
	Repeat	445 – 474	30	ANK 11
	Repeat	481 – 510	30	ANK 12
	Repeat	513 – 542	30	ANK 13
	Repeat	547 – 576	30	ANK 14
	Repeat	579 – 609	31	ANK 15
Amino acid modifications
	Glycosylation	747	1	N-linked (GlcNAc...) Potential
	Glycosylation	753	1	N-linked (GlcNAc...) Potential
	Disulfide bond	192 ↔ 665		Alternate By similarity
	Disulfide bond	462 ↔ 665		Alternate By similarity
	Disulfide bond	608 ↔ 621		Alternate By similarity
	Disulfide bond	621 ↔ 665		Alternate By similarity
Natural variations
	Natural variant	3	1	R → C. Ref.1 Corresponds to variant rs13268757 [ dbSNP | Ensembl ].		VAR_020660
	Natural variant	58	1	R → T. Ref.1 Corresponds to variant rs16937976 [ dbSNP | Ensembl ].		VAR_047471
	Natural variant	179	1	E → K. Corresponds to variant rs920829 [ dbSNP | Ensembl ].		VAR_020661
	Natural variant	186	1	K → N. Ref.1 Corresponds to variant rs7819749 [ dbSNP | Ensembl ].		VAR_020662
	Natural variant	1018	1	H → R. Corresponds to variant rs959976 [ dbSNP | Ensembl ].		VAR_020663

Sequences

	Sequence		Length	Mass (Da)
	O75762 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: 283BF31BC77CF71B	FASTA	1,119	127,501
	10 20 30 40 50 60 MKRSLRKMWR PGEKKEPQGV VYEDVPDDTE DFKESLKVVF EGSAYGLQNF NKQKKLKRCD 70 80 90 100 110 120 DMDTFFLHYA AAEGQIELME KITRDSSLEV LHEMDDYGNT PLHCAVEKNQ IESVKFLLSR 130 140 150 160 170 180 GANPNLRNFN MMAPLHIAVQ GMNNEVMKVL LEHRTIDVNL EGENGNTAVI IACTTNNSEA 190 200 210 220 230 240 LQILLKKGAK PCKSNKWGCF PIHQAAFSGS KECMEIILRF GEEHGYSRQL HINFMNNGKA 250 260 270 280 290 300 TPLHLAVQNG DLEMIKMCLD NGAQIDPVEK GRCTAIHFAA TQGATEIVKL MISSYSGSVD 310 320 330 340 350 360 IVNTTDGCHE TMLHRASLFD HHELADYLIS VGADINKIDS EGRSPLILAT ASASWNIVNL 370 380 390 400 410 420 LLSKGAQVDI KDNFGRNFLH LTVQQPYGLK NLRPEFMQMQ QIKELVMDED NDGCTPLHYA 430 440 450 460 470 480 CRQGGPGSVN NLLGFNVSIH SKSKDKKSPL HFAASYGRIN TCQRLLQDIS DTRLLNEGDL 490 500 510 520 530 540 HGMTPLHLAA KNGHDKVVQL LLKKGALFLS DHNGWTALHH ASMGGYTQTM KVILDTNLKC 550 560 570 580 590 600 TDRLDEDGNT ALHFAAREGH AKAVALLLSH NADIVLNKQQ ASFLHLALHN KRKEVVLTII 610 620 630 640 650 660 RSKRWDECLK IFSHNSPGNK CPITEMIEYL PECMKVLLDF CMLHSTEDKS CRDYYIEYNF 670 680 690 700 710 720 KYLQCPLEFT KKTPTQDVIY EPLTALNAMV QNNRIELLNH PVCKEYLLMK WLAYGFRAHM 730 740 750 760 770 780 MNLGSYCLGL IPMTILVVNI KPGMAFNSTG IINETSDHSE ILDTTNSYLI KTCMILVFLS 790 800 810 820 830 840 SIFGYCKEAG QIFQQKRNYF MDISNVLEWI IYTTGIIFVL PLFVEIPAHL QWQCGAIAVY 850 860 870 880 890 900 FYWMNFLLYL QRFENCGIFI VMLEVILKTL LRSTVVFIFL LLAFGLSFYI LLNLQDPFSS 910 920 930 940 950 960 PLLSIIQTFS MMLGDINYRE SFLEPYLRNE LAHPVLSFAQ LVSFTIFVPI VLMNLLIGLA 970 980 990 1000 1010 1020 VGDIAEVQKH ASLKRIAMQV ELHTSLEKKL PLWFLRKVDQ KSTIVYPNKP RSGGMLFHIF 1030 1040 1050 1060 1070 1080 CFLFCTGEIR QEIPNADKSL EMEILKQKYR LKDLTFLLEK QHELIKLIIQ KMEIISETED 1090 1100 1110 DDSHCSFQDR FKKEQMEQRN SRWNTVLRAV KAKTHHLEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An ankyrin-like protein with transmembrane domains is specifically lost after oncogenic transformation of human fibroblasts." Jaquemar D., Schenker T., Trueb B. J. Biol. Chem. 274:7325-7333(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS CYS-3; THR-58 AND ASN-186. Tissue: Lung.
[2]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y10601 mRNA. Translation: CAA71610.1. AC022867 Genomic DNA. No translation available.
IPI	IPI00015963.
RefSeq	NP_015628.2. NM_007332.2.
UniGene	Hs.716816.
3D structure databases
ProteinModelPortal	O75762.
SMR	O75762. Positions 35-699.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000262209.
Protein family/group databases
TCDB	1.A.4.6.3. transient receptor potential Ca2+ channel (TRP-CC) family.
PTM databases
PhosphoSite	O75762.
Proteomic databases
PaxDb	O75762.
PRIDE	O75762.
Protocols and materials databases
DNASU	8989.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262209; ENSP00000262209; ENSG00000104321.
GeneID	8989.
KEGG	hsa:8989.
UCSC	uc003xza.3. human.
Organism-specific databases
CTD	8989.
GeneCards	GC08M072983.
H-InvDB	HIX0034373. HIX0124652.
HGNC	HGNC:497. TRPA1.
HPA	HPA026630.
MIM	604775. gene.
neXtProt	NX_O75762.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000044486.
HOVERGEN	HBG059027.
InParanoid	O75762.
KO	K04984.
OMA	WLAYGFR.
OrthoDB	EOG49KFPT.
Gene expression databases
ArrayExpress	O75762.
Bgee	O75762.
CleanEx	HS_TRPA1.
Genevestigator	O75762.
GermOnline	ENSG00000104321. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 4 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans_dom. [Graphical view]
Pfam	PF00023. Ank. 10 hits. PF00520. Ion_trans. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 14 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 2 hits.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 9 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O75762.
ChEMBL	CHEMBL6007.
DrugBank	DB00825. Menthol.
GenomeRNAi	8989.
NextBio	33711.
SOURCE	Search...

Entry information

Entry name

TRPA1_HUMAN

Accession

Primary (citable) accession number: O75762
Secondary accession number(s): A6NIN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.