Unreviewed, UniProtKB/TrEMBL O75758 (O75758_HUMAN)

Names and origin

Protein names	Recommended name:     Pyruvate kinase RuleBase RU000504V4     EC=2.7.1.40 RuleBase RU000504V4
Organism	Homo sapiens (Human) EMBL BAA02515.1
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate. RuleBase RU000504V4
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. RuleBase RU000504V4
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis RuleBase RU000504V4
Ligand	Magnesium RuleBase RU000504V4 Pyruvate EMBL BAA02515.1
Molecular function	Kinase RuleBase RU000504V4 EMBL BAA02515.1 Transferase
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: Compara cellular response to insulin stimulus Inferred from electronic annotation. Source: Compara glycolysis Non-traceable author statement. Source: UniProtKB pyruvate biosynthetic process Inferred from electronic annotation. Source: Compara response to ATP Inferred from electronic annotation. Source: Compara response to cAMP Inferred from electronic annotation. Source: Compara response to glucose stimulus Inferred from electronic annotation. Source: Compara response to heat Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to lithium ion Inferred from electronic annotation. Source: Compara response to nutrient Inferred from electronic annotation. Source: Compara response to organic cyclic substance Inferred from electronic annotation. Source: Compara response to other organism Inferred from electronic annotation. Source: Compara
Cellular component	cytosol Inferred from electronic annotation. Source: Compara soluble fraction Inferred from electronic annotation. Source: Compara
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O75758-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 29BCFF812F1181A0	FASTA	599	64,502
	10 20 30 40 50 60 MSIQENISSL QLRSWVSKSQ RDLAKSILIG APGVPLTTQQ CGADPQRGRP REVCSGMEGP 70 80 90 100 110 120 AGYLRRASVA QLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG 130 140 150 160 170 180 PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL 190 200 210 220 230 240 DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG 250 260 270 280 290 300 RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG 310 320 330 340 350 360 VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV 370 380 390 400 410 420 ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKARPTR AETSDVANAV 430 440 450 460 470 480 LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT 490 500 510 520 530 540 AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL 550 560 570 580 590 LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS

References

[1]	"Structural analysis of human pyruvate kinase L-gene and identification of the promoter activity in erythroid cells." Kanno H., Fujii H., Miwa S. Biochem. Biophys. Res. Commun. 188:516-523(1992) [PubMed: 1445295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Human liver type pyruvate kinase: complete amino acid sequence and the expression in mammalian cells." Tani K., Fujii H., Nagata S., Miwa S. Proc. Natl. Acad. Sci. U.S.A. 85:1792-1795(1988) [PubMed: 3126495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13243 Genomic DNA. Translation: BAA02515.1.
IPI	IPI00743713.
PIR	KIHUPL. I52269.
UniGene	Hs.95990
3D structure databases
HSSP	HSSP built from PDB template 1LIU based on UniProtKB P30613.
SMR	O75758. Positions 82-598.
ModBase	Search...
Protein-protein interaction databases
STRING	O75758.
Proteomic databases
PRIDE	O75758.
Genome annotation databases
Ensembl	ENST00000423816; ENSP00000408891; ENSG00000143627; Homo sapiens. [Genome view]
Organism-specific databases
HGNC	HGNC:9020. PKLR.
Phylogenomic databases
eggNOG	prNOG13400.
HOGENOM	HBG734376.
HOVERGEN	O75758.
PhylomeDB	O75758.
Gene expression databases
ArrayExpress	O75758.
Bgee	O75758.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR011037. Pyrv_Knase_b-brl-like. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C-like. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O75758_HUMAN

Accession

Primary (citable) accession number: O75758

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1998
Last sequence update:	November 1, 1998
Last modified:	January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.