O75751 (S22A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 22 member 3

Alternative name(s):
Extraneuronal monoamine transporter
Short name=EMT
Organic cation transporter 3

Gene names

Name:	SLC22A3
Synonyms:	EMTH, OCT3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain. Ref.1 Ref.6
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen. Ref.1 Ref.5 Ref.6
Sequence similarities	Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dopamine transport Inferred from electronic annotation. Source: Compara drug transmembrane transport Traceable author statement. Source: Reactome histamine uptake Inferred from electronic annotation. Source: Compara regulation of appetite Inferred from electronic annotation. Source: Compara small molecule metabolic process Traceable author statement. Source: Reactome toxin transport Inferred from electronic annotation. Source: GOC
Cellular_component	integral to plasma membrane Traceable author statement Ref.5. Source: ProtInc
Molecular_function	dopamine transmembrane transporter activity Inferred from electronic annotation. Source: Compara quaternary ammonium group transmembrane transporter activity Inferred from direct assay Ref.6. Source: MGI toxin transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	2	EBI-1752674,EBI-375543

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Solute carrier family 22 member 3		PRO_0000220503
Regions
	Transmembrane	21 – 41	21	Helical; Potential
	Transmembrane	182 – 202	21	Helical; Potential
	Transmembrane	241 – 261	21	Helical; Potential
	Transmembrane	269 – 289	21	Helical; Potential
	Transmembrane	381 – 401	21	Helical; Potential
	Transmembrane	468 – 488	21	Helical; Potential
	Transmembrane	498 – 518	21	Helical; Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	44	1	T → M. Corresponds to variant rs8187715 [ dbSNP | Ensembl ].		VAR_020351
	Natural variant	116	1	A → S. Corresponds to variant rs8187717 [ dbSNP | Ensembl ].		VAR_020352

Sequences

	Sequence		Length	Mass (Da)
	O75751 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C3CA2D77DD21C658	FASTA	556	61,280
	10 20 30 40 50 60 MPSFDEALQR VGEFGRFQRR VFLLLCLTGV TFAFLFVGVV FLGTQPDHYW CRGPSAAALA 70 80 90 100 110 120 ERCGWSPEEE WNRTAPASRG PEPPERRGRC QRYLLEAAND SASATSALSC ADPLAAFPNR 130 140 150 160 170 180 SAPLVPCRGG WRYAQAHSTI VSEFDLVCVN AWMLDLTQAI LNLGFLTGAF TLGYAADRYG 190 200 210 220 230 240 RIVIYLLSCL GVGVTGVVVA FAPNFPVFVI FRFLQGVFGK GTWMTCYVIV TEIVGSKQRR 250 260 270 280 290 300 IVGIVIQMFF TLGIIILPGI AYFIPNWQGI QLAITLPSFL FLLYYWVVPE SPRWLITRKK 310 320 330 340 350 360 GDKALQILRR IAKCNGKYLS SNYSEITVTD EEVSNPSFLD LVRTPQMRKC TLILMFAWFT 370 380 390 400 410 420 SAVVYQGLVM RLGIIGGNLY IDFFISGVVE LPGALLILLT IERLGRRLPF AASNIVAGVA 430 440 450 460 470 480 CLVTAFLPEG IAWLRTTVAT LGRLGITMAF EIVYLVNSEL YPTTLRNFGV SLCSGLCDFG 490 500 510 520 530 540 GIIAPFLLFR LAAVWLELPL IIFGILASIC GGLVMLLPET KGIALPETVD DVEKLGSPHS 550 CKCGRNKKTP VSRSHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification of the corticosterone-sensitive extraneuronal catecholamine transporter." Gruendemann D., Schechinger B., Rappold G.A., Schoemig E. Nat. Neurosci. 1:349-351(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Kidney.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Cloning of the mouse and human solute carrier 22a3 (Slc22a3/SLC22A3) identifies a conserved cluster of three organic cation transporters on mouse chromosome 17 and human 6q26-q27." Verhaagh S., Schweifer N., Barlow D.P., Zwart R. Genomics 55:209-218(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 513-556, TISSUE SPECIFICITY.
[6]	"Structure, function, and regional distribution of the organic cation transporter OCT3 in the kidney." Wu X., Huang W., Ganapathy M.E., Wang H., Kekuda R., Conway S.J., Leibach F.H., Ganapathy V. Am. J. Physiol. 279:F449-F458(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001417 mRNA. Translation: CAA04751.1. AK314590 mRNA. Translation: BAG37164.1. AL591069, AL355506 Genomic DNA. Translation: CAI16539.1. AL355506, AL591069 Genomic DNA. Translation: CAI20340.1. CH471051 Genomic DNA. Translation: EAW47599.1. AF078749 mRNA. Translation: AAD20977.1.
IPI	IPI00872448.
RefSeq	NP_068812.1. NM_021977.3.
UniGene	Hs.567337.
3D structure databases
ProteinModelPortal	O75751.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75751. 2 interactions.
STRING	9606.ENSP00000275300.
PTM databases
PhosphoSite	O75751.
Proteomic databases
PaxDb	O75751.
PRIDE	O75751.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000275300; ENSP00000275300; ENSG00000146477.
GeneID	6581.
KEGG	hsa:6581.
UCSC	uc003qti.3. human.
Organism-specific databases
CTD	6581.
GeneCards	GC06P160739.
HGNC	HGNC:10967. SLC22A3.
HPA	HPA029750.
MIM	604842. gene.
neXtProt	NX_O75751.
PharmGKB	PA330.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000234568.
HOVERGEN	HBG061545.
KO	K08200.
OrthoDB	EOG4K6G3X.
PhylomeDB	O75751.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	O75751.
Bgee	O75751.
CleanEx	HS_SLC22A3.
Genevestigator	O75751.
GermOnline	ENSG00000146477. Homo sapiens.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004749. Orgcat_transp. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00898. 2A0119. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 1 hit. PS00217. SUGAR_TRANSPORT_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC22A3. human.
GenomeRNAi	6581.
NextBio	25609.
SOURCE	Search...

Entry information

Entry name

S22A3_HUMAN

Accession

Primary (citable) accession number: O75751
Secondary accession number(s): Q5SYN6, Q9UP02

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	November 1, 1998
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.