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O75751

- S22A3_HUMAN

UniProt

O75751 - S22A3_HUMAN

Protein

Solute carrier family 22 member 3

Gene

SLC22A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.2 Publications

    GO - Molecular functioni

    1. dopamine transmembrane transporter activity Source: Ensembl
    2. organic cation transmembrane transporter activity Source: MGI
    3. protein binding Source: IntAct
    4. quaternary ammonium group transmembrane transporter activity Source: MGI
    5. toxin transporter activity Source: Ensembl

    GO - Biological processi

    1. dopamine transport Source: Ensembl
    2. drug transmembrane transport Source: Reactome
    3. histamine uptake Source: Ensembl
    4. organic cation transport Source: MGI
    5. quaternary ammonium group transport Source: MGI
    6. regulation of appetite Source: Ensembl
    7. small molecule metabolic process Source: Reactome
    8. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_120801. Abacavir transmembrane transport.
    REACT_22357. Organic cation transport.

    Protein family/group databases

    TCDBi2.A.1.19.24. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 3
    Alternative name(s):
    Extraneuronal monoamine transporter
    Short name:
    EMT
    Organic cation transporter 3
    Gene namesi
    Name:SLC22A3
    Synonyms:EMTH, OCT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:10967. SLC22A3.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. membrane Source: ProtInc
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA330.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 556556Solute carrier family 22 member 3PRO_0000220503Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiO75751.
    PaxDbiO75751.
    PRIDEiO75751.

    PTM databases

    PhosphoSiteiO75751.

    Expressioni

    Tissue specificityi

    Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.3 Publications

    Gene expression databases

    ArrayExpressiO75751.
    BgeeiO75751.
    CleanExiHS_SLC22A3.
    GenevestigatoriO75751.

    Organism-specific databases

    HPAiHPA029750.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABL1P005192EBI-1752674,EBI-375543

    Protein-protein interaction databases

    IntActiO75751. 2 interactions.
    STRINGi9606.ENSP00000275300.

    Structurei

    3D structure databases

    ProteinModelPortaliO75751.
    SMRiO75751. Positions 163-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei269 – 28921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei381 – 40121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei468 – 48821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei498 – 51821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    HOGENOMiHOG000234568.
    HOVERGENiHBG061545.
    KOiK08200.
    OrthoDBiEOG78PV8N.
    PhylomeDBiO75751.
    TreeFamiTF315847.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O75751-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSFDEALQR VGEFGRFQRR VFLLLCLTGV TFAFLFVGVV FLGTQPDHYW    50
    CRGPSAAALA ERCGWSPEEE WNRTAPASRG PEPPERRGRC QRYLLEAAND 100
    SASATSALSC ADPLAAFPNR SAPLVPCRGG WRYAQAHSTI VSEFDLVCVN 150
    AWMLDLTQAI LNLGFLTGAF TLGYAADRYG RIVIYLLSCL GVGVTGVVVA 200
    FAPNFPVFVI FRFLQGVFGK GTWMTCYVIV TEIVGSKQRR IVGIVIQMFF 250
    TLGIIILPGI AYFIPNWQGI QLAITLPSFL FLLYYWVVPE SPRWLITRKK 300
    GDKALQILRR IAKCNGKYLS SNYSEITVTD EEVSNPSFLD LVRTPQMRKC 350
    TLILMFAWFT SAVVYQGLVM RLGIIGGNLY IDFFISGVVE LPGALLILLT 400
    IERLGRRLPF AASNIVAGVA CLVTAFLPEG IAWLRTTVAT LGRLGITMAF 450
    EIVYLVNSEL YPTTLRNFGV SLCSGLCDFG GIIAPFLLFR LAAVWLELPL 500
    IIFGILASIC GGLVMLLPET KGIALPETVD DVEKLGSPHS CKCGRNKKTP 550
    VSRSHL 556
    Length:556
    Mass (Da):61,280
    Last modified:November 1, 1998 - v1
    Checksum:iC3CA2D77DD21C658
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti44 – 441T → M.
    Corresponds to variant rs8187715 [ dbSNP | Ensembl ].
    VAR_020351
    Natural varianti116 – 1161A → S.
    Corresponds to variant rs8187717 [ dbSNP | Ensembl ].
    VAR_020352

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001417 mRNA. Translation: CAA04751.1.
    AK314590 mRNA. Translation: BAG37164.1.
    AL591069, AL355506 Genomic DNA. Translation: CAI16539.1.
    AL355506, AL591069 Genomic DNA. Translation: CAI20340.1.
    CH471051 Genomic DNA. Translation: EAW47599.1.
    AF078749 mRNA. Translation: AAD20977.1.
    CCDSiCCDS5277.1.
    RefSeqiNP_068812.1. NM_021977.3.
    UniGeneiHs.567337.

    Genome annotation databases

    EnsembliENST00000275300; ENSP00000275300; ENSG00000146477.
    GeneIDi6581.
    KEGGihsa:6581.
    UCSCiuc003qti.4. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001417 mRNA. Translation: CAA04751.1 .
    AK314590 mRNA. Translation: BAG37164.1 .
    AL591069 , AL355506 Genomic DNA. Translation: CAI16539.1 .
    AL355506 , AL591069 Genomic DNA. Translation: CAI20340.1 .
    CH471051 Genomic DNA. Translation: EAW47599.1 .
    AF078749 mRNA. Translation: AAD20977.1 .
    CCDSi CCDS5277.1.
    RefSeqi NP_068812.1. NM_021977.3.
    UniGenei Hs.567337.

    3D structure databases

    ProteinModelPortali O75751.
    SMRi O75751. Positions 163-527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O75751. 2 interactions.
    STRINGi 9606.ENSP00000275300.

    Chemistry

    ChEMBLi CHEMBL2073673.

    Protein family/group databases

    TCDBi 2.A.1.19.24. the major facilitator superfamily (mfs).

    PTM databases

    PhosphoSitei O75751.

    Proteomic databases

    MaxQBi O75751.
    PaxDbi O75751.
    PRIDEi O75751.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000275300 ; ENSP00000275300 ; ENSG00000146477 .
    GeneIDi 6581.
    KEGGi hsa:6581.
    UCSCi uc003qti.4. human.

    Organism-specific databases

    CTDi 6581.
    GeneCardsi GC06P160739.
    HGNCi HGNC:10967. SLC22A3.
    HPAi HPA029750.
    MIMi 604842. gene.
    neXtProti NX_O75751.
    PharmGKBi PA330.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0477.
    HOGENOMi HOG000234568.
    HOVERGENi HBG061545.
    KOi K08200.
    OrthoDBi EOG78PV8N.
    PhylomeDBi O75751.
    TreeFami TF315847.

    Enzyme and pathway databases

    Reactomei REACT_120801. Abacavir transmembrane transport.
    REACT_22357. Organic cation transport.

    Miscellaneous databases

    ChiTaRSi SLC22A3. human.
    GeneWikii SLC22A3.
    GenomeRNAii 6581.
    NextBioi 25609.
    PROi O75751.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75751.
    Bgeei O75751.
    CleanExi HS_SLC22A3.
    Genevestigatori O75751.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00898. 2A0119. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular identification of the corticosterone-sensitive extraneuronal catecholamine transporter."
      Gruendemann D., Schechinger B., Rappold G.A., Schoemig E.
      Nat. Neurosci. 1:349-351(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Tissue: Kidney.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Tongue.
    3. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Cloning of the mouse and human solute carrier 22a3 (Slc22a3/SLC22A3) identifies a conserved cluster of three organic cation transporters on mouse chromosome 17 and human 6q26-q27."
      Verhaagh S., Schweifer N., Barlow D.P., Zwart R.
      Genomics 55:209-218(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 513-556, TISSUE SPECIFICITY.
    6. "Structure, function, and regional distribution of the organic cation transporter OCT3 in the kidney."
      Wu X., Huang W., Ganapathy M.E., Wang H., Kekuda R., Conway S.J., Leibach F.H., Ganapathy V.
      Am. J. Physiol. 279:F449-F458(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
      Tissue: Kidney.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiS22A3_HUMAN
    AccessioniPrimary (citable) accession number: O75751
    Secondary accession number(s): Q5SYN6, Q9UP02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2002
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3