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Protein

WD repeat and HMG-box DNA-binding protein 1

Gene

WDHD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1016 – 1079HMG boxPROSITE-ProRule annotationAdd BLAST64

GO - Molecular functioni

  • DNA binding Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33162-MONOMER.
SignaLinkiO75717.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat and HMG-box DNA-binding protein 1
Alternative name(s):
Acidic nucleoplasmic DNA-binding protein 1
Short name:
And-1
Gene namesi
Name:WDHD1
Synonyms:AND1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:23170. WDHD1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11169.
OpenTargetsiENSG00000198554.
PharmGKBiPA134988782.

Polymorphism and mutation databases

BioMutaiWDHD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513381 – 1129WD repeat and HMG-box DNA-binding protein 1Add BLAST1129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei333PhosphoserineCombined sources1
Modified residuei374PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Modified residuei671N6-acetyllysineBy similarity1
Modified residuei824PhosphothreonineCombined sources1
Modified residuei826PhosphothreonineCombined sources1
Modified residuei868PhosphoserineCombined sources1
Modified residuei917PhosphoserineCombined sources1
Modified residuei919PhosphoserineCombined sources1
Modified residuei932PhosphoserineCombined sources1
Modified residuei962N6-acetyllysineCombined sources1
Modified residuei984PhosphoserineCombined sources1
Modified residuei1041PhosphoserineCombined sources1
Modified residuei1090PhosphoserineCombined sources1
Cross-linki1127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki1127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75717.
MaxQBiO75717.
PaxDbiO75717.
PeptideAtlasiO75717.
PRIDEiO75717.

PTM databases

iPTMnetiO75717.
PhosphoSitePlusiO75717.

Expressioni

Gene expression databases

BgeeiENSG00000198554.
CleanExiHS_WDHD1.
ExpressionAtlasiO75717. baseline and differential.
GenevisibleiO75717. HS.

Organism-specific databases

HPAiHPA001122.

Interactioni

Subunit structurei

Interacts with the polymerase alpha catalytic subunit POLA1. Interacts with MCM10. Interacts with DNA2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KAT2AQ928305EBI-3951691,EBI-477622

Protein-protein interaction databases

BioGridi116340. 43 interactors.
IntActiO75717. 8 interactors.
STRINGi9606.ENSP00000353793.

Structurei

Secondary structure

11129
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1021 – 1036Combined sources16
Helixi1043 – 1054Combined sources12
Beta strandi1055 – 1057Combined sources3
Helixi1059 – 1068Combined sources10
Beta strandi1071 – 1076Combined sources6
Helixi1081 – 1083Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D7LNMR-A1017-1084[»]
ProteinModelPortaliO75717.
SMRiO75717.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75717.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 50WD 1Add BLAST40
Repeati52 – 91WD 2Add BLAST40
Repeati92 – 131WD 3Add BLAST40
Repeati134 – 173WD 4Add BLAST40
Repeati184 – 223WD 5Add BLAST40
Repeati228 – 267WD 6Add BLAST40
Repeati271 – 310WD 7Add BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi829 – 836Glu-rich (acidic)8
Compositional biasi862 – 878Asp/Glu-rich (acidic)Add BLAST17

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1274. Eukaryota.
ENOG410XSJV. LUCA.
GeneTreeiENSGT00390000002030.
HOGENOMiHOG000170076.
HOVERGENiHBG074190.
InParanoidiO75717.
KOiK11274.
OMAiWNSVGIV.
OrthoDBiEOG091G084W.
PhylomeDBiO75717.
TreeFamiTF105988.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR009071. HMG_box_dom.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF12341. Mcl1_mid. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75717-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPATRKPMRY GHTEGHTEVC FDDSGSFIVT CGSDGDVRIW EDLDDDDPKF
60 70 80 90 100
INVGEKAYSC ALKSGKLVTA VSNNTIQVHT FPEGVPDGIL TRFTTNANHV
110 120 130 140 150
VFNGDGTKIA AGSSDFLVKI VDVMDSSQQK TFRGHDAPVL SLSFDPKDIF
160 170 180 190 200
LASASCDGSV RVWQISDQTC AISWPLLQKC NDVINAKSIC RLAWQPKSGK
210 220 230 240 250
LLAIPVEKSV KLYRRESWSH QFDLSDNFIS QTLNIVTWSP CGQYLAAGSI
260 270 280 290 300
NGLIIVWNVE TKDCMERVKH EKGYAICGLA WHPTCGRISY TDAEGNLGLL
310 320 330 340 350
ENVCDPSGKT SSSKVSSRVE KDYNDLFDGD DMSNAGDFLN DNAVEIPSFS
360 370 380 390 400
KGIINDDEDD EDLMMASGRP RQRSHILEDD ENSVDISMLK TGSSLLKEEE
410 420 430 440 450
EDGQEGSIHN LPLVTSQRPF YDGPMPTPRQ KPFQSGSTPL HLTHRFMVWN
460 470 480 490 500
SIGIIRCYND EQDNAIDVEF HDTSIHHATH LSNTLNYTIA DLSHEAILLA
510 520 530 540 550
CESTDELASK LHCLHFSSWD SSKEWIIDLP QNEDIEAICL GQGWAAAATS
560 570 580 590 600
ALLLRLFTIG GVQKEVFSLA GPVVSMAGHG EQLFIVYHRG TGFDGDQCLG
610 620 630 640 650
VQLLELGKKK KQILHGDPLP LTRKSYLAWI GFSAEGTPCY VDSEGIVRML
660 670 680 690 700
NRGLGNTWTP ICNTREHCKG KSDHYWVVGI HENPQQLRCI PCKGSRFPPT
710 720 730 740 750
LPRPAVAILS FKLPYCQIAT EKGQMEEQFW RSVIFHNHLD YLAKNGYEYE
760 770 780 790 800
ESTKNQATKE QQELLMKMLA LSCKLEREFR CVELADLMTQ NAVNLAIKYA
810 820 830 840 850
SRSRKLILAQ KLSELAVEKA AELTATQVEE EEEEEDFRKK LNAGYSNTAT
860 870 880 890 900
EWSQPRFRNQ VEEDAEDSGE ADDEEKPEIH KPGQNSFSKS TNSSDVSAKS
910 920 930 940 950
GAVTFSSQGR VNPFKVSASS KEPAMSMNSA RSTNILDNMG KSSKKSTALS
960 970 980 990 1000
RTTNNEKSPI IKPLIPKPKP KQASAASYFQ KRNSQTNKTE EVKEENLKNV
1010 1020 1030 1040 1050
LSETPAICPP QNTENQRPKT GFQMWLEENR SNILSDNPDF SDEADIIKEG
1060 1070 1080 1090 1100
MIRFRVLSTE ERKVWANKAK GETASEGTEA KKRKRVVDES DETENQEEKA
1110 1120
KENLNLSKKQ KPLDFSTNQK LSAFAFKQE
Length:1,129
Mass (Da):125,967
Last modified:November 1, 1998 - v1
Checksum:iDD3E3613CA6AE70A
GO
Isoform 2 (identifier: O75717-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:1,006
Mass (Da):112,705
Checksum:i430FD72EAB983984
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti612Q → K in AAH43349 (PubMed:15489334).Curated1
Sequence conflicti612Q → K in AAH00622 (PubMed:15489334).Curated1
Sequence conflicti613I → K in AAH00622 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053422338F → L.Corresponds to variant rs8020032dbSNPEnsembl.1
Natural variantiVAR_053423411L → P.Corresponds to variant rs17128116dbSNPEnsembl.1
Natural variantiVAR_0621001102E → K.Corresponds to variant rs41309252dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0547751 – 123Missing in isoform 2. CuratedAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006266 mRNA. Translation: CAA06932.1.
AL160471 Genomic DNA. No translation available.
BC000622 mRNA. Translation: AAH00622.1.
BC043349 mRNA. Translation: AAH43349.1.
BC063041 mRNA. Translation: AAH63041.1.
CCDSiCCDS41955.1. [O75717-2]
CCDS9721.1. [O75717-1]
RefSeqiNP_001008397.1. NM_001008396.2. [O75717-2]
NP_009017.1. NM_007086.3. [O75717-1]
UniGeneiHs.385998.

Genome annotation databases

EnsembliENST00000360586; ENSP00000353793; ENSG00000198554. [O75717-1]
ENST00000420358; ENSP00000399349; ENSG00000198554. [O75717-2]
GeneIDi11169.
KEGGihsa:11169.
UCSCiuc001xbm.3. human. [O75717-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006266 mRNA. Translation: CAA06932.1.
AL160471 Genomic DNA. No translation available.
BC000622 mRNA. Translation: AAH00622.1.
BC043349 mRNA. Translation: AAH43349.1.
BC063041 mRNA. Translation: AAH63041.1.
CCDSiCCDS41955.1. [O75717-2]
CCDS9721.1. [O75717-1]
RefSeqiNP_001008397.1. NM_001008396.2. [O75717-2]
NP_009017.1. NM_007086.3. [O75717-1]
UniGeneiHs.385998.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D7LNMR-A1017-1084[»]
ProteinModelPortaliO75717.
SMRiO75717.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116340. 43 interactors.
IntActiO75717. 8 interactors.
STRINGi9606.ENSP00000353793.

PTM databases

iPTMnetiO75717.
PhosphoSitePlusiO75717.

Polymorphism and mutation databases

BioMutaiWDHD1.

Proteomic databases

EPDiO75717.
MaxQBiO75717.
PaxDbiO75717.
PeptideAtlasiO75717.
PRIDEiO75717.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360586; ENSP00000353793; ENSG00000198554. [O75717-1]
ENST00000420358; ENSP00000399349; ENSG00000198554. [O75717-2]
GeneIDi11169.
KEGGihsa:11169.
UCSCiuc001xbm.3. human. [O75717-1]

Organism-specific databases

CTDi11169.
DisGeNETi11169.
GeneCardsiWDHD1.
HGNCiHGNC:23170. WDHD1.
HPAiHPA001122.
MIMi608126. gene.
neXtProtiNX_O75717.
OpenTargetsiENSG00000198554.
PharmGKBiPA134988782.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1274. Eukaryota.
ENOG410XSJV. LUCA.
GeneTreeiENSGT00390000002030.
HOGENOMiHOG000170076.
HOVERGENiHBG074190.
InParanoidiO75717.
KOiK11274.
OMAiWNSVGIV.
OrthoDBiEOG091G084W.
PhylomeDBiO75717.
TreeFamiTF105988.

Enzyme and pathway databases

BioCyciZFISH:G66-33162-MONOMER.
SignaLinkiO75717.

Miscellaneous databases

ChiTaRSiWDHD1. human.
EvolutionaryTraceiO75717.
GeneWikiiWDHD1.
GenomeRNAii11169.
PROiO75717.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198554.
CleanExiHS_WDHD1.
ExpressionAtlasiO75717. baseline and differential.
GenevisibleiO75717. HS.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR009071. HMG_box_dom.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF12341. Mcl1_mid. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWDHD1_HUMAN
AccessioniPrimary (citable) accession number: O75717
Secondary accession number(s): C9JW18, F6W0U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.