Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

WD repeat and HMG-box DNA-binding protein 1

Gene

WDHD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1016 – 1079HMG boxPROSITE-ProRule annotationAdd BLAST64

GO - Molecular functioni

  • DNA binding Source: ProtInc

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

SignaLinkiO75717.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat and HMG-box DNA-binding protein 1
Alternative name(s):
Acidic nucleoplasmic DNA-binding protein 1
Short name:
And-1
Gene namesi
Name:WDHD1
Synonyms:AND1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000198554.11.
HGNCiHGNC:23170. WDHD1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11169.
OpenTargetsiENSG00000198554.
PharmGKBiPA134988782.

Polymorphism and mutation databases

BioMutaiWDHD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513381 – 1129WD repeat and HMG-box DNA-binding protein 1Add BLAST1129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei333PhosphoserineCombined sources1
Modified residuei374PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1
Modified residuei387PhosphoserineCombined sources1
Cross-linki397Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei671N6-acetyllysineBy similarity1
Modified residuei824PhosphothreonineCombined sources1
Modified residuei826PhosphothreonineCombined sources1
Modified residuei868PhosphoserineCombined sources1
Modified residuei917PhosphoserineCombined sources1
Modified residuei919PhosphoserineCombined sources1
Modified residuei932PhosphoserineCombined sources1
Modified residuei962N6-acetyllysineCombined sources1
Modified residuei984PhosphoserineCombined sources1
Modified residuei1041PhosphoserineCombined sources1
Modified residuei1090PhosphoserineCombined sources1
Cross-linki1127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki1127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75717.
MaxQBiO75717.
PaxDbiO75717.
PeptideAtlasiO75717.
PRIDEiO75717.

PTM databases

iPTMnetiO75717.
PhosphoSitePlusiO75717.

Expressioni

Gene expression databases

BgeeiENSG00000198554.
CleanExiHS_WDHD1.
ExpressionAtlasiO75717. baseline and differential.
GenevisibleiO75717. HS.

Organism-specific databases

HPAiHPA001122.

Interactioni

Subunit structurei

Interacts with the polymerase alpha catalytic subunit POLA1. Interacts with MCM10. Interacts with DNA2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KAT2AQ928305EBI-3951691,EBI-477622

Protein-protein interaction databases

BioGridi116340. 50 interactors.
CORUMiO75717.
IntActiO75717. 9 interactors.
STRINGi9606.ENSP00000353793.

Structurei

Secondary structure

11129
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 15Combined sources4
Beta strandi18 – 21Combined sources4
Beta strandi28 – 31Combined sources4
Beta strandi35 – 42Combined sources8
Beta strandi49 – 52Combined sources4
Beta strandi57 – 63Combined sources7
Beta strandi66 – 71Combined sources6
Turni72 – 74Combined sources3
Beta strandi75 – 80Combined sources6
Turni81 – 83Combined sources3
Beta strandi86 – 92Combined sources7
Beta strandi97 – 102Combined sources6
Beta strandi106 – 113Combined sources8
Beta strandi118 – 122Combined sources5
Beta strandi125 – 132Combined sources8
Beta strandi139 – 144Combined sources6
Beta strandi148 – 155Combined sources8
Beta strandi160 – 164Combined sources5
Turni165 – 168Combined sources4
Beta strandi169 – 174Combined sources6
Helixi183 – 185Combined sources3
Beta strandi196 – 198Combined sources3
Beta strandi201 – 206Combined sources6
Beta strandi209 – 214Combined sources6
Turni215 – 217Combined sources3
Beta strandi220 – 224Combined sources5
Beta strandi233 – 238Combined sources6
Beta strandi242 – 249Combined sources8
Beta strandi254 – 258Combined sources5
Turni259 – 261Combined sources3
Beta strandi264 – 268Combined sources5
Beta strandi276 – 281Combined sources6
Beta strandi283 – 292Combined sources10
Beta strandi295 – 302Combined sources8
Beta strandi442 – 449Combined sources8
Beta strandi451 – 458Combined sources8
Beta strandi465 – 472Combined sources8
Turni473 – 475Combined sources3
Beta strandi479 – 481Combined sources3
Beta strandi489 – 492Combined sources4
Beta strandi497 – 501Combined sources5
Beta strandi505 – 507Combined sources3
Beta strandi510 – 514Combined sources5
Beta strandi519 – 521Combined sources3
Beta strandi524 – 528Combined sources5
Beta strandi535 – 540Combined sources6
Beta strandi542 – 549Combined sources8
Beta strandi552 – 558Combined sources7
Beta strandi563 – 568Combined sources6
Beta strandi573 – 579Combined sources7
Beta strandi582 – 588Combined sources7
Beta strandi599 – 618Combined sources20
Beta strandi627 – 632Combined sources6
Beta strandi638 – 642Combined sources5
Beta strandi645 – 650Combined sources6
Helixi652 – 654Combined sources3
Beta strandi658 – 663Combined sources6
Helixi664 – 667Combined sources4
Beta strandi674 – 681Combined sources8
Turni682 – 685Combined sources4
Beta strandi686 – 697Combined sources12
Beta strandi700 – 702Combined sources3
Beta strandi707 – 709Combined sources3
Turni716 – 719Combined sources4
Helixi721 – 734Combined sources14
Helixi737 – 744Combined sources8
Helixi751 – 774Combined sources24
Helixi778 – 786Combined sources9
Helixi790 – 800Combined sources11
Turni801 – 804Combined sources4
Helixi806 – 823Combined sources18
Helixi1021 – 1036Combined sources16
Helixi1043 – 1054Combined sources12
Beta strandi1055 – 1057Combined sources3
Helixi1059 – 1068Combined sources10
Beta strandi1071 – 1076Combined sources6
Helixi1081 – 1083Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D7LNMR-A1017-1084[»]
5GVAX-ray1.85A1-330[»]
5GVBX-ray2.75A416-850[»]
ProteinModelPortaliO75717.
SMRiO75717.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75717.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 50WD 1Add BLAST40
Repeati52 – 91WD 2Add BLAST40
Repeati92 – 131WD 3Add BLAST40
Repeati134 – 173WD 4Add BLAST40
Repeati184 – 223WD 5Add BLAST40
Repeati228 – 267WD 6Add BLAST40
Repeati271 – 310WD 7Add BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi829 – 836Glu-rich (acidic)8
Compositional biasi862 – 878Asp/Glu-rich (acidic)Add BLAST17

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1274. Eukaryota.
ENOG410XSJV. LUCA.
GeneTreeiENSGT00390000002030.
HOGENOMiHOG000170076.
HOVERGENiHBG074190.
InParanoidiO75717.
KOiK11274.
OMAiVIFGSEK.
OrthoDBiEOG091G084W.
PhylomeDBiO75717.
TreeFamiTF105988.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR011042. 6-blade_b-propeller_TolB-like.
IPR024977. Apc4_WD40_dom.
IPR009071. HMG_box_dom.
IPR036910. HMG_box_dom_sf.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF12894. ANAPC4_WD40. 1 hit.
PF12341. Mcl1_mid. 1 hit.
PF00400. WD40. 2 hits.
SMARTiView protein in SMART
SM00398. HMG. 1 hit.
SM00320. WD40. 5 hits.
SUPFAMiSSF47095. SSF47095. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS50118. HMG_BOX_2. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75717-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPATRKPMRY GHTEGHTEVC FDDSGSFIVT CGSDGDVRIW EDLDDDDPKF
60 70 80 90 100
INVGEKAYSC ALKSGKLVTA VSNNTIQVHT FPEGVPDGIL TRFTTNANHV
110 120 130 140 150
VFNGDGTKIA AGSSDFLVKI VDVMDSSQQK TFRGHDAPVL SLSFDPKDIF
160 170 180 190 200
LASASCDGSV RVWQISDQTC AISWPLLQKC NDVINAKSIC RLAWQPKSGK
210 220 230 240 250
LLAIPVEKSV KLYRRESWSH QFDLSDNFIS QTLNIVTWSP CGQYLAAGSI
260 270 280 290 300
NGLIIVWNVE TKDCMERVKH EKGYAICGLA WHPTCGRISY TDAEGNLGLL
310 320 330 340 350
ENVCDPSGKT SSSKVSSRVE KDYNDLFDGD DMSNAGDFLN DNAVEIPSFS
360 370 380 390 400
KGIINDDEDD EDLMMASGRP RQRSHILEDD ENSVDISMLK TGSSLLKEEE
410 420 430 440 450
EDGQEGSIHN LPLVTSQRPF YDGPMPTPRQ KPFQSGSTPL HLTHRFMVWN
460 470 480 490 500
SIGIIRCYND EQDNAIDVEF HDTSIHHATH LSNTLNYTIA DLSHEAILLA
510 520 530 540 550
CESTDELASK LHCLHFSSWD SSKEWIIDLP QNEDIEAICL GQGWAAAATS
560 570 580 590 600
ALLLRLFTIG GVQKEVFSLA GPVVSMAGHG EQLFIVYHRG TGFDGDQCLG
610 620 630 640 650
VQLLELGKKK KQILHGDPLP LTRKSYLAWI GFSAEGTPCY VDSEGIVRML
660 670 680 690 700
NRGLGNTWTP ICNTREHCKG KSDHYWVVGI HENPQQLRCI PCKGSRFPPT
710 720 730 740 750
LPRPAVAILS FKLPYCQIAT EKGQMEEQFW RSVIFHNHLD YLAKNGYEYE
760 770 780 790 800
ESTKNQATKE QQELLMKMLA LSCKLEREFR CVELADLMTQ NAVNLAIKYA
810 820 830 840 850
SRSRKLILAQ KLSELAVEKA AELTATQVEE EEEEEDFRKK LNAGYSNTAT
860 870 880 890 900
EWSQPRFRNQ VEEDAEDSGE ADDEEKPEIH KPGQNSFSKS TNSSDVSAKS
910 920 930 940 950
GAVTFSSQGR VNPFKVSASS KEPAMSMNSA RSTNILDNMG KSSKKSTALS
960 970 980 990 1000
RTTNNEKSPI IKPLIPKPKP KQASAASYFQ KRNSQTNKTE EVKEENLKNV
1010 1020 1030 1040 1050
LSETPAICPP QNTENQRPKT GFQMWLEENR SNILSDNPDF SDEADIIKEG
1060 1070 1080 1090 1100
MIRFRVLSTE ERKVWANKAK GETASEGTEA KKRKRVVDES DETENQEEKA
1110 1120
KENLNLSKKQ KPLDFSTNQK LSAFAFKQE
Length:1,129
Mass (Da):125,967
Last modified:November 1, 1998 - v1
Checksum:iDD3E3613CA6AE70A
GO
Isoform 2 (identifier: O75717-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available. Derived from EST data.
Show »
Length:1,006
Mass (Da):112,705
Checksum:i430FD72EAB983984
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti612Q → K in AAH43349 (PubMed:15489334).Curated1
Sequence conflicti612Q → K in AAH00622 (PubMed:15489334).Curated1
Sequence conflicti613I → K in AAH00622 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053422338F → L. Corresponds to variant dbSNP:rs8020032Ensembl.1
Natural variantiVAR_053423411L → P. Corresponds to variant dbSNP:rs17128116Ensembl.1
Natural variantiVAR_0621001102E → K. Corresponds to variant dbSNP:rs41309252Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0547751 – 123Missing in isoform 2. CuratedAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006266 mRNA. Translation: CAA06932.1.
AL160471 Genomic DNA. No translation available.
BC000622 mRNA. Translation: AAH00622.1.
BC043349 mRNA. Translation: AAH43349.1.
BC063041 mRNA. Translation: AAH63041.1.
CCDSiCCDS41955.1. [O75717-2]
CCDS9721.1. [O75717-1]
RefSeqiNP_001008397.1. NM_001008396.2. [O75717-2]
NP_009017.1. NM_007086.3. [O75717-1]
UniGeneiHs.385998.

Genome annotation databases

EnsembliENST00000360586; ENSP00000353793; ENSG00000198554. [O75717-1]
ENST00000420358; ENSP00000399349; ENSG00000198554. [O75717-2]
GeneIDi11169.
KEGGihsa:11169.
UCSCiuc001xbm.3. human. [O75717-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiWDHD1_HUMAN
AccessioniPrimary (citable) accession number: O75717
Secondary accession number(s): C9JW18, F6W0U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: November 1, 1998
Last modified: November 22, 2017
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references