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Protein

Serine/threonine-protein kinase 16

Gene

STK16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei148Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiO75716.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 16 (EC:2.7.11.1)
Alternative name(s):
Myristoylated and palmitoylated serine/threonine-protein kinase
Short name:
MPSK
Protein kinase PKL12
TGF-beta-stimulated factor 1
Short name:
TSF-1
Tyrosine-protein kinase STK16 (EC:2.7.10.2)
hPSK
Gene namesi
Name:STK16
Synonyms:MPSK1, PKL12, TSF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:11394. STK16.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Loss of myristoylation. 1 Publication1
Mutagenesisi6C → S: Loss of palmitoylation. 1 Publication1
Mutagenesisi8C → S: Loss of palmitoylation. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000115661.
PharmGKBiPA36202.

Chemistry databases

ChEMBLiCHEMBL3938.
GuidetoPHARMACOLOGYi2213.

Polymorphism and mutation databases

BioMutaiSTK16.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000867012 – 305Serine/threonine-protein kinase 16Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi6S-palmitoyl cysteine1 Publication1
Lipidationi8S-palmitoyl cysteine1 Publication1
Modified residuei185Phosphothreonine; by autocatalysis1 Publication1
Modified residuei197Phosphoserine; by autocatalysis1 Publication1
Modified residuei198Phosphotyrosine; by autocatalysis1 Publication1

Post-translational modificationi

Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198.1 Publication
It is uncertain whether palmitoylation is on Cys-6 and/or Cys-8.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO75716.
PeptideAtlasiO75716.
PRIDEiO75716.

PTM databases

iPTMnetiO75716.
PhosphoSitePlusiO75716.
SwissPalmiO75716.

Expressioni

Tissue specificityi

Ubiquitously expressed at very low levels.1 Publication

Gene expression databases

BgeeiENSG00000115661.
CleanExiHS_STK16.
ExpressionAtlasiO75716. baseline and differential.
GenevisibleiO75716. HS.

Organism-specific databases

HPAiHPA029450.

Interactioni

Subunit structurei

Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114144. 69 interactors.
DIPiDIP-29598N.
IntActiO75716. 56 interactors.
MINTiMINT-3001680.
STRINGi9606.ENSP00000379964.

Chemistry databases

BindingDBiO75716.

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 15Combined sources3
Beta strandi18 – 27Combined sources10
Beta strandi32 – 39Combined sources8
Turni40 – 42Combined sources3
Beta strandi45 – 55Combined sources11
Helixi56 – 70Combined sources15
Beta strandi82 – 88Combined sources7
Beta strandi91 – 99Combined sources9
Helixi106 – 114Combined sources9
Turni115 – 117Combined sources3
Helixi122 – 141Combined sources20
Helixi151 – 153Combined sources3
Beta strandi154 – 156Combined sources3
Beta strandi162 – 164Combined sources3
Beta strandi171 – 173Combined sources3
Beta strandi175 – 179Combined sources5
Helixi180 – 193Combined sources14
Helixi196 – 198Combined sources3
Helixi201 – 203Combined sources3
Beta strandi208 – 212Combined sources5
Helixi215 – 230Combined sources16
Helixi236 – 240Combined sources5
Helixi245 – 250Combined sources6
Helixi263 – 272Combined sources10
Helixi277 – 279Combined sources3
Helixi283 – 292Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BUJX-ray2.60A/B13-305[»]
ProteinModelPortaliO75716.
SMRiO75716.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75716.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 202Activation loopAdd BLAST37

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2345. Eukaryota.
ENOG410YN06. LUCA.
GeneTreeiENSGT00550000075037.
HOVERGENiHBG054359.
InParanoidiO75716.
KOiK08856.
PhylomeDBiO75716.
TreeFamiTF350433.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O75716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHALCVCSR GTVIIDNKRY LFIQKLGEGG FSYVDLVEGL HDGHFYALKR
60 70 80 90 100
ILCHEQQDRE EAQREADMHR LFNHPNILRL VAYCLRERGA KHEAWLLLPF
110 120 130 140 150
FKRGTLWNEI ERLKDKGNFL TEDQILWLLL GICRGLEAIH AKGYAHRDLK
160 170 180 190 200
PTNILLGDEG QPVLMDLGSM NQACIHVEGS RQALTLQDWA AQRCTISYRA
210 220 230 240 250
PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ KGDSVALAVQ
260 270 280 290 300
NQLSIPQSPR HSSALRQLLN SMMTVDPHQR PHIPLLLSQL EALQPPAPGQ

HTTQI
Length:305
Mass (Da):34,656
Last modified:January 23, 2007 - v4
Checksum:i593B2AEB8505009C
GO

Sequence cautioni

The sequence AAV38392 differs from that shown. Reason: Frameshift at position 305.Curated
The sequence CAA06700 differs from that shown. Reason: Frameshift at position 305.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59R → L in AAV38392 (Ref. 6) Curated1
Sequence conflicti180S → P in BAF85383 (PubMed:14702039).Curated1
Sequence conflicti213D → G in CAA06700 (PubMed:9712705).Curated1
Sequence conflicti213D → G in CAA09387 (Ref. 4) Curated1
Sequence conflicti221L → F in AAC28337 (PubMed:10364453).Curated1
Sequence conflicti222G → S in CAA09387 (Ref. 4) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04114041H → R1 PublicationCorresponds to variant dbSNP:rs34799131Ensembl.1
Natural variantiVAR_04114155E → K1 PublicationCorresponds to variant dbSNP:rs35947471Ensembl.1
Natural variantiVAR_04114277I → V1 PublicationCorresponds to variant dbSNP:rs34282267Ensembl.1
Natural variantiVAR_041143266R → W3 PublicationsCorresponds to variant dbSNP:rs17849638Ensembl.1
Natural variantiVAR_041144277P → L1 PublicationCorresponds to variant dbSNP:rs35454203Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005791 mRNA. Translation: CAA06700.1. Frameshift.
AF060798 mRNA. Translation: AAC28337.1.
AB020739 mRNA. Translation: BAB16311.1.
AJ010872 mRNA. Translation: CAA09387.1.
AF203910 mRNA. Translation: AAG23728.1.
BT019585 mRNA. Translation: AAV38392.1. Frameshift.
AK292694 mRNA. Translation: BAF85383.1.
CR407675 mRNA. Translation: CAG28603.1.
BC002618 mRNA. Translation: AAH02618.1.
BC053998 mRNA. Translation: AAH53998.1.
CCDSiCCDS42822.1.
RefSeqiNP_001008910.1. NM_001008910.3.
NP_001317142.1. NM_001330213.1.
UniGeneiHs.153003.

Genome annotation databases

EnsembliENST00000396738; ENSP00000379964; ENSG00000115661.
ENST00000409638; ENSP00000386928; ENSG00000115661.
GeneIDi8576.
KEGGihsa:8576.
UCSCiuc002vko.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005791 mRNA. Translation: CAA06700.1. Frameshift.
AF060798 mRNA. Translation: AAC28337.1.
AB020739 mRNA. Translation: BAB16311.1.
AJ010872 mRNA. Translation: CAA09387.1.
AF203910 mRNA. Translation: AAG23728.1.
BT019585 mRNA. Translation: AAV38392.1. Frameshift.
AK292694 mRNA. Translation: BAF85383.1.
CR407675 mRNA. Translation: CAG28603.1.
BC002618 mRNA. Translation: AAH02618.1.
BC053998 mRNA. Translation: AAH53998.1.
CCDSiCCDS42822.1.
RefSeqiNP_001008910.1. NM_001008910.3.
NP_001317142.1. NM_001330213.1.
UniGeneiHs.153003.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BUJX-ray2.60A/B13-305[»]
ProteinModelPortaliO75716.
SMRiO75716.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114144. 69 interactors.
DIPiDIP-29598N.
IntActiO75716. 56 interactors.
MINTiMINT-3001680.
STRINGi9606.ENSP00000379964.

Chemistry databases

BindingDBiO75716.
ChEMBLiCHEMBL3938.
GuidetoPHARMACOLOGYi2213.

PTM databases

iPTMnetiO75716.
PhosphoSitePlusiO75716.
SwissPalmiO75716.

Polymorphism and mutation databases

BioMutaiSTK16.

Proteomic databases

PaxDbiO75716.
PeptideAtlasiO75716.
PRIDEiO75716.

Protocols and materials databases

DNASUi8576.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396738; ENSP00000379964; ENSG00000115661.
ENST00000409638; ENSP00000386928; ENSG00000115661.
GeneIDi8576.
KEGGihsa:8576.
UCSCiuc002vko.3. human.

Organism-specific databases

CTDi8576.
GeneCardsiSTK16.
HGNCiHGNC:11394. STK16.
HPAiHPA029450.
MIMi604719. gene.
neXtProtiNX_O75716.
OpenTargetsiENSG00000115661.
PharmGKBiPA36202.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2345. Eukaryota.
ENOG410YN06. LUCA.
GeneTreeiENSGT00550000075037.
HOVERGENiHBG054359.
InParanoidiO75716.
KOiK08856.
PhylomeDBiO75716.
TreeFamiTF350433.

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiO75716.

Miscellaneous databases

ChiTaRSiSTK16. human.
EvolutionaryTraceiO75716.
GeneWikiiSTK16.
GenomeRNAii8576.
PROiO75716.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115661.
CleanExiHS_STK16.
ExpressionAtlasiO75716. baseline and differential.
GenevisibleiO75716. HS.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTK16_HUMAN
AccessioniPrimary (citable) accession number: O75716
Secondary accession number(s): A8K9H9
, Q5U0F8, Q96KI2, Q9BUH4, Q9UEN3, Q9UP78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: February 15, 2017
This is version 167 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.