O75635 (SPB7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serpin B7

Alternative name(s):
Megsin
TP55

Gene names

Name:

SERPINB7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Predominantly expressed in mesangial cells.
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75635-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75635-2)

The sequence of this isoform differs from the canonical sequence as follows:
     57-73: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Serpin B7		PRO_0000094108
Sites
	Site	347 – 348	2	Reactive bond By similarity
Amino acid modifications
	Modified residue	217	1	Phosphoserine Ref.6
	Modified residue	223	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	57 – 73	17	Missing in isoform 2.		VSP_044711
	Natural variant	266	1	R → Q. Corresponds to variant rs17782413 [ dbSNP | Ensembl ].		VAR_034512
Experimental info
	Sequence conflict	155	1	K → E in BAG62480. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 9A2CDB6C63CFF605	FASTA	380	42,905
	10 20 30 40 50 60 MASLAAANAE FCFNLFREMD DNQGNGNVFF SSLSLFAALA LVRLGAQDDS LSQIDKLLHV 70 80 90 100 110 120 NTASGYGNSS NSQSGLQSQL KRVFSDINAS HKDYDLSIVN GLFAEKVYGF HKDYIECAEK 130 140 150 160 170 180 LYDAKVERVD FTNHLEDTRR NINKWVENET HGKIKNVIGE GGISSSAVMV LVNAVYFKGK 190 200 210 220 230 240 WQSAFTKSET INCHFKSPKC SGKAVAMMHQ ERKFNLSVIE DPSMKILELR YNGGINMYVL 250 260 270 280 290 300 LPENDLSEIE NKLTFQNLME WTNPRRMTSK YVEVFFPQFK IEKNYEMKQY LRALGLKDIF 310 320 330 340 350 360 DESKADLSGI ASGGRLYISR MMHKSYIEVT EEGTEATAAT GSNIVEKQLP QSTLFRADHP 370 380 FLFVIRKDDI ILFSGKVSCP
	Isoform 2 [UniParc]. Checksum: 9E9C813FC804B165	FASTA	363	41,174
	10 20 30 40 50 60 MASLAAANAE FCFNLFREMD DNQGNGNVFF SSLSLFAALA LVRLGAQDDS LSQIDKSGLQ 70 80 90 100 110 120 SQLKRVFSDI NASHKDYDLS IVNGLFAEKV YGFHKDYIEC AEKLYDAKVE RVDFTNHLED 130 140 150 160 170 180 TRRNINKWVE NETHGKIKNV IGEGGISSSA VMVLVNAVYF KGKWQSAFTK SETINCHFKS 190 200 210 220 230 240 PKCSGKAVAM MHQERKFNLS VIEDPSMKIL ELRYNGGINM YVLLPENDLS EIENKLTFQN 250 260 270 280 290 300 LMEWTNPRRM TSKYVEVFFP QFKIEKNYEM KQYLRALGLK DIFDESKADL SGIASGGRLY 310 320 330 340 350 360 ISRMMHKSYI EVTEEGTEAT AATGSNIVEK QLPQSTLFRA DHPFLFVIRK DDIILFSGKV SCP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, cDNA cloning, and characterization of a new serpin with megakaryocyte maturation activity." Tsujimoto M., Tsuruoka N., Ishida N., Kurihara T., Iwasa F., Yamashiro K., Rogi T., Kodama S., Katsuragi N., Adachi M., Katayama T., Nakao M., Yamaichi K., Hashino J., Haruyama M., Miura K., Nakanishi T., Nakazato H. , Teramura M., Mizoguchi H., Yamaguchi N. J. Biol. Chem. 272:15373-15380(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A mesangium-predominant gene, megsin, is a new serpin upregulated in IgA nephropathy." Miyata T., Nangaku M., Suzuki D., Inagi R., Uragami K., Sakai H., Okubo K., Kurokawa K. J. Clin. Invest. 102:828-836(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Mesangial cell.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217 AND SER-223, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D88575 mRNA. Translation: BAA31232.1. AF027866 mRNA. Translation: AAC64506.1. AK300828 mRNA. Translation: BAG62480.1. AC069356 Genomic DNA. No translation available. AC072051 Genomic DNA. No translation available. BC069417 mRNA. Translation: AAH69417.1. BC069442 mRNA. Translation: AAH69442.1. BC069547 mRNA. Translation: AAH69547.1. BC106743 mRNA. Translation: AAI06744.1. BC106744 mRNA. Translation: AAI06745.1.
IPI	IPI00021555. IPI01010407.
RefSeq	NP_001035237.1. NM_001040147.2. NP_001248759.1. NM_001261830.1. NP_001248760.1. NM_001261831.1. NP_003775.1. NM_003784.3.
UniGene	Hs.138202. Hs.742388.
3D structure databases
ProteinModelPortal	O75635.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000337212.
Protein family/group databases
MEROPS	I04.012.
PTM databases
PhosphoSite	O75635.
Proteomic databases
PaxDb	O75635.
PRIDE	O75635.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336429; ENSP00000337212; ENSG00000166396. ENST00000398019; ENSP00000381101; ENSG00000166396. ENST00000540675; ENSP00000444572; ENSG00000166396. ENST00000546027; ENSP00000444861; ENSG00000166396.
GeneID	8710.
KEGG	hsa:8710.
UCSC	uc002ljl.3. human.
Organism-specific databases
CTD	8710.
GeneCards	GC18P061394.
HGNC	HGNC:13902. SERPINB7.
HPA	HPA024200.
MIM	603357. gene.
neXtProt	NX_O75635.
PharmGKB	PA37825.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238519.
HOVERGEN	HBG005957.
InParanoid	O75635.
KO	K13964.
OMA	ISMYIML.
OrthoDB	EOG4ZPDVJ.
PhylomeDB	O75635.
Gene expression databases
ArrayExpress	O75635.
Bgee	O75635.
CleanEx	HS_SERPINB7.
Genevestigator	O75635.
GermOnline	ENSG00000166396. Homo sapiens.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8710.
NextBio	32665.
SOURCE	Search...

Entry information

Entry name

SPB7_HUMAN

Accession

Primary (citable) accession number: O75635
Secondary accession number(s): B4DUW8 , F5GZC0, Q1ED45, Q3KPG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.