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O75628 (REM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein REM 1
Alternative name(s):
GTPase-regulating endothelial cell sprouting
Rad and Gem-like GTP-binding protein 1
Gene names
Name:REM1
Synonyms:GES, REM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes endothelial cell sprouting and actin cytoskeletal reorganization. May be involved in angiogenesis. May function in Ca2+ signaling.

Subunit structure

In vitro, interacts with calmodulin in a calcium-dependent manner.

Tissue specificity

Most highly expressed in the endothelial lining of the blood vessels in uterus and heart. Lower levels found in spleen, lymph node, kidney and testis. Also found in cells with secretory function such as the islets of Langerhans, lobule/duct epithelium in the breast, bile duct epithelium in the liver, surface epithelium in the endometrial glands of the uterus, colon mucosa and acinar cells in the pancreas and the prostate.

Sequence similarities

Belongs to the small GTPase superfamily. RGK family.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   LigandCalmodulin-binding
GTP-binding
Nucleotide-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from electronic annotation. Source: InterPro

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmembrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 298298GTP-binding protein REM 1
PRO_0000122481

Regions

Nucleotide binding87 – 948GTP By similarity
Nucleotide binding195 – 1984GTP By similarity
Region268 – 28720Calmodulin-binding By similarity
Compositional bias241 – 28848Arg-rich

Natural variations

Natural variant281H → R. Ref.3 Ref.4
Corresponds to variant rs1006459 [ dbSNP | Ensembl ].
VAR_049498
Natural variant451R → W.
Corresponds to variant rs59730832 [ dbSNP | Ensembl ].
VAR_061232
Natural variant591P → A.
Corresponds to variant rs2233829 [ dbSNP | Ensembl ].
VAR_049499

Experimental info

Mutagenesis941T → N: No endothelial cell sprouting. Ref.2
Sequence conflict2091E → V in AAC33132. Ref.1

Secondary structure

............................ 298
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O75628 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: E7CCE776FFD42CD7

FASTA29832,947
        10         20         30         40         50         60 
MTLNTEQEAK TPLHRRASTP LPLSPRGHQP GRLSTVPSTQ SQHPRLGQSA SLNPPTQKPS 

        70         80         90        100        110        120 
PAPDDWSSES SDSEGSWEAL YRVVLLGDPG VGKTSLASLF AGKQERDLHE QLGEDVYERT 

       130        140        150        160        170        180 
LTVDGEDTTL VVVDTWEAEK LDKSWSQESC LQGGSAYVIV YSIADRGSFE SASELRIQLR 

       190        200        210        220        230        240 
RTHQADHVPI ILVGNKADLA RCREVSVEEG RACAVVFDCK FIETSATLQH NVAELFEGVV 

       250        260        270        280        290 
RQLRLRRRDS AAKEPPAPRR PASLAQRARR FLARLTARSA RRRALKARSK SCHNLAVL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the human Rem gene."
Finlin B.S., Andres D.A.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Ges, a human GTPase of the Rad/Gem/Kir family, promotes endothelial cell sprouting and cytoskeleton reorganization."
Pan J.Y., Fieles W.E., White A.M., Egerton M.M., Silberstein D.S.
J. Cell Biol. 149:1107-1116(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF THR-94.
Tissue: Lymph node.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-28.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-28.
Tissue: Caudate nucleus.
[5]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF084465 mRNA. Translation: AAC33132.1.
AF152863 mRNA. Translation: AAF74212.1.
BT020078 mRNA. Translation: AAV38881.1.
BT020079 mRNA. Translation: AAV38882.1.
AK313060 mRNA. Translation: BAG35890.1.
AL121751 Genomic DNA. Translation: CAB90274.1.
CH471077 Genomic DNA. Translation: EAW76439.1.
CH471077 Genomic DNA. Translation: EAW76440.1.
CH471077 Genomic DNA. Translation: EAW76442.1.
BC039813 mRNA. Translation: AAH39813.1.
CCDSCCDS13181.1.
RefSeqNP_054731.2. NM_014012.5.
UniGeneHs.247729.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2NZJX-ray2.50A/B/C/D79-251[»]
ProteinModelPortalO75628.
SMRO75628. Positions 79-247.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118781. 6 interactions.
STRING9606.ENSP00000201979.

PTM databases

PhosphoSiteO75628.

Proteomic databases

PaxDbO75628.
PRIDEO75628.

Protocols and materials databases

DNASU28954.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000201979; ENSP00000201979; ENSG00000088320.
GeneID28954.
KEGGhsa:28954.
UCSCuc002wwa.3. human.

Organism-specific databases

CTD28954.
GeneCardsGC20P030063.
HGNCHGNC:15922. REM1.
HPAHPA049821.
MIM610388. gene.
neXtProtNX_O75628.
PharmGKBPA34324.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000246961.
HOVERGENHBG104899.
InParanoidO75628.
KOK07847.
OMAKLDESWS.
OrthoDBEOG7Q8CP8.
PhylomeDBO75628.
TreeFamTF314379.

Gene expression databases

BgeeO75628.
CleanExHS_REM1.
GenevestigatorO75628.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR017358. Small_GTPase_GEM/REM/Rad.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PIRSFPIRSF038017. GTP-binding_GEM. 1 hit.
PRINTSPR00449. RASTRNSFRMNG.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO75628.
GenomeRNAi28954.
NextBio51769.
PROO75628.
SOURCESearch...

Entry information

Entry nameREM1_HUMAN
AccessionPrimary (citable) accession number: O75628
Secondary accession number(s): E1P5L1 expand/collapse secondary AC list , Q5TZR7, Q5TZR8, Q9NP57
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 7, 2003
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM