Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTPase Era, mitochondrial

Gene

ERAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi120 – 1278GTPSequence analysis
Nucleotide bindingi167 – 1715GTPSequence analysis
Nucleotide bindingi236 – 2394GTPSequence analysis

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB
  • ribosomal small subunit binding Source: UniProtKB
  • rRNA binding Source: UniProtKB

GO - Biological processi

  • ribosomal small subunit assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Era, mitochondrial
Short name:
H-ERA
Short name:
hERA
Alternative name(s):
Conserved ERA-like GTPase
Short name:
CEGA
ERA-W
ERA-like protein 1
Gene namesi
Name:ERAL1
Synonyms:HERA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:3424. ERAL1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27843.

Polymorphism and mutation databases

BioMutaiERAL1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343MitochondrionSequence analysisAdd
BLAST
Chaini44 – 437394GTPase Era, mitochondrialPRO_0000180081Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75616.
MaxQBiO75616.
PaxDbiO75616.
PeptideAtlasiO75616.
PRIDEiO75616.

PTM databases

iPTMnetiO75616.
PhosphoSiteiO75616.

Expressioni

Gene expression databases

BgeeiO75616.
CleanExiHS_ERAL1.
ExpressionAtlasiO75616. baseline and differential.
GenevisibleiO75616. HS.

Organism-specific databases

HPAiHPA021425.
HPA024423.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP44Q9C0F13EBI-6393536,EBI-744115

Protein-protein interaction databases

BioGridi117666. 29 interactions.
IntActiO75616. 12 interactions.
MINTiMINT-3046801.
STRINGi9606.ENSP00000254928.

Structurei

3D structure databases

ProteinModelPortaliO75616.
SMRiO75616. Positions 114-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 330219Era-type GAdd
BLAST
Domaini360 – 43778KH type-2Add
BLAST

Sequence similaritiesi

Contains 1 KH type-2 domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1423. Eukaryota.
COG1159. LUCA.
GeneTreeiENSGT00390000013800.
HOVERGENiHBG051495.
InParanoidiO75616.
OMAiPAVSMHR.
OrthoDBiEOG7XWPNP.
PhylomeDBiO75616.
TreeFamiTF321650.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00367. GTPase_Era.
InterProiIPR030388. G_ERA_dom.
IPR005662. GTP-bd_Era.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR009019. KH_prok-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54814. SSF54814. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51713. G_ERA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HERA-A (identifier: O75616-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPSWRGAR LVQSVLRVWQ VGPHVARERV IPFSSLLGFQ RRCVSCVAGS
60 70 80 90 100
AFSGPRLASA SRSNGQGSAL DHFLGFSQPD SSVTPCVPAV SMNRDEQDVL
110 120 130 140 150
LVHHPDMPEN SRVLRVVLLG APNAGKSTLS NQLLGRKVFP VSRKVHTTRC
160 170 180 190 200
QALGVITEKE TQVILLDTPG IISPGKQKRH HLELSLLEDP WKSMESADLV
210 220 230 240 250
VVLVDVSDKW TRNQLSPQLL RCLTKYSQIP SVLVMNKVDC LKQKSVLLEL
260 270 280 290 300
TAALTEGVVN GKKLKMRQAF HSHPGTHCPS PAVKDPNTQS VGNPQRIGWP
310 320 330 340 350
HFKEIFMLSA LSQEDVKTLK QYLLTQAQPG PWEYHSAVLT SQTPEEICAN
360 370 380 390 400
IIREKLLEHL PQEVPYNVQQ KTAVWEEGPG GELVIQQKLL VPKESYVKLL
410 420 430
IGPKGHVISQ IAQEAGHDLM DIFLCDVDIR LSVKLLK
Length:437
Mass (Da):48,350
Last modified:January 4, 2005 - v2
Checksum:i5C2454A1D9AFF5AA
GO
Isoform HERA-B (identifier: O75616-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     257-437: GVVNGKKLKM...DIRLSVKLLK → AIPSDTGPARALGVPQCSPH

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:276
Mass (Da):30,040
Checksum:i4D0A9F7F0D657072
GO

Sequence cautioni

The sequence AAG12978.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → V in AAC31603 (PubMed:10945472).Curated
Sequence conflicti1 – 11M → V in AAC31604 (PubMed:10945472).Curated
Sequence conflicti15 – 151V → A in AAC31603 (PubMed:10945472).Curated
Sequence conflicti15 – 151V → A in AAC31604 (PubMed:10945472).Curated
Sequence conflicti17 – 182RV → KI in AAG12978 (Ref. 3) Curated
Sequence conflicti190 – 1901P → T in BAB56112 (PubMed:11733036).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei257 – 437181GVVNG…VKLLK → AIPSDTGPARALGVPQCSPH in isoform HERA-B. 1 PublicationVSP_001453Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082657 mRNA. Translation: AAC31603.1.
AF082658 mRNA. Translation: AAC31604.1.
AB049388 mRNA. Translation: BAB56112.1.
AY007435 mRNA. Translation: AAG12978.1. Different initiation.
AK023342 mRNA. Translation: BAG51183.1.
AC024267 Genomic DNA. No translation available.
CH471159 Genomic DNA. Translation: EAW51147.1.
BC019094 mRNA. Translation: AAH19094.1.
CCDSiCCDS11244.1. [O75616-1]
RefSeqiNP_001304914.1. NM_001317985.1.
NP_001304915.1. NM_001317986.1.
NP_005693.1. NM_005702.3. [O75616-1]
UniGeneiHs.3426.

Genome annotation databases

EnsembliENST00000254928; ENSP00000254928; ENSG00000132591. [O75616-1]
GeneIDi26284.
KEGGihsa:26284.
UCSCiuc002hcy.2. human. [O75616-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF082657 mRNA. Translation: AAC31603.1.
AF082658 mRNA. Translation: AAC31604.1.
AB049388 mRNA. Translation: BAB56112.1.
AY007435 mRNA. Translation: AAG12978.1. Different initiation.
AK023342 mRNA. Translation: BAG51183.1.
AC024267 Genomic DNA. No translation available.
CH471159 Genomic DNA. Translation: EAW51147.1.
BC019094 mRNA. Translation: AAH19094.1.
CCDSiCCDS11244.1. [O75616-1]
RefSeqiNP_001304914.1. NM_001317985.1.
NP_001304915.1. NM_001317986.1.
NP_005693.1. NM_005702.3. [O75616-1]
UniGeneiHs.3426.

3D structure databases

ProteinModelPortaliO75616.
SMRiO75616. Positions 114-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117666. 29 interactions.
IntActiO75616. 12 interactions.
MINTiMINT-3046801.
STRINGi9606.ENSP00000254928.

PTM databases

iPTMnetiO75616.
PhosphoSiteiO75616.

Polymorphism and mutation databases

BioMutaiERAL1.

Proteomic databases

EPDiO75616.
MaxQBiO75616.
PaxDbiO75616.
PeptideAtlasiO75616.
PRIDEiO75616.

Protocols and materials databases

DNASUi26284.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254928; ENSP00000254928; ENSG00000132591. [O75616-1]
GeneIDi26284.
KEGGihsa:26284.
UCSCiuc002hcy.2. human. [O75616-1]

Organism-specific databases

CTDi26284.
GeneCardsiERAL1.
HGNCiHGNC:3424. ERAL1.
HPAiHPA021425.
HPA024423.
MIMi607435. gene.
neXtProtiNX_O75616.
PharmGKBiPA27843.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1423. Eukaryota.
COG1159. LUCA.
GeneTreeiENSGT00390000013800.
HOVERGENiHBG051495.
InParanoidiO75616.
OMAiPAVSMHR.
OrthoDBiEOG7XWPNP.
PhylomeDBiO75616.
TreeFamiTF321650.

Miscellaneous databases

ChiTaRSiERAL1. human.
GenomeRNAii26284.
PROiO75616.
SOURCEiSearch...

Gene expression databases

BgeeiO75616.
CleanExiHS_ERAL1.
ExpressionAtlasiO75616. baseline and differential.
GenevisibleiO75616. HS.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00367. GTPase_Era.
InterProiIPR030388. G_ERA_dom.
IPR005662. GTP-bd_Era.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR009019. KH_prok-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54814. SSF54814. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51713. G_ERA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and preliminary characterization of the human and mouse homologues of the bacterial cell cycle gene era."
    Britton R.A., Chen S.M., Wallis D., Koeuth T., Powell B.S., Shaffer L.G., Largaespada D., Jenkins N.A., Copeland N.G., Court D.L., Lupski J.R.
    Genomics 67:78-82(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS HERA-A AND HERA-B).
  2. "Mammalian homologue of E. coli Ras-like GTPase (ERA) is a possible apoptosis regulator with RNA binding activity."
    Akiyama T., Gohda J., Shibata S., Nomura Y., Azuma S., Ohmori Y., Sugano S., Arai H., Yamamoto T., Inoue J.
    Genes Cells 6:987-1001(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM HERA-A), RNA-BINDING.
  3. "cDNA cloning and characterization of human ERA."
    Chen S., Chen N., Ji Z.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM HERA-A).
    Tissue: Colon.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HERA-A).
    Tissue: Ovary.
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HERA-A).
    Tissue: Brain.
  8. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
  9. "Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit."
    Dennerlein S., Rozanska A., Wydro M., Chrzanowska-Lightowlers Z.M., Lightowlers R.N.
    Biochem. J. 430:551-558(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING.
  10. "ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation."
    Uchiumi T., Ohgaki K., Yagi M., Aoki Y., Sakai A., Matsumoto S., Kang D.
    Nucleic Acids Res. 38:5554-5568(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Erythroleukemia.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiERAL1_HUMAN
AccessioniPrimary (citable) accession number: O75616
Secondary accession number(s): B3KN21
, C9JEC6, O75617, Q8WUY4, Q96LE2, Q96TC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 4, 2005
Last modified: July 6, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.