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Protein

Acyl-protein thioesterase 1

Gene

LYPLA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity toward KCNMA1. Has low lysophospholipase activity.2 Publications

Catalytic activityi

Palmitoyl-protein + H2O = palmitate + protein.1 Publication

Enzyme regulationi

Inhibited by palmostatin-B, leading to impair depalmitoylating of Ras.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Charge relay system1 Publication1
Active sitei174Charge relay system1 Publication1
Active sitei208Charge relay system1 Publication1

GO - Molecular functioni

  • lipase activity Source: CACAO
  • lysophospholipase activity Source: ProtInc
  • palmitoyl-(protein) hydrolase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciZFISH:HS04458-MONOMER.
BRENDAi3.1.2.22. 2681.
ReactomeiR-HSA-203615. eNOS activation.

Protein family/group databases

ESTHERihuman-LYPLA1. LYsophospholipase_carboxylesterase.
MEROPSiS09.941.

Chemistry databases

SwissLipidsiSLP:000000734.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-protein thioesterase 1 (EC:3.1.2.-)
Short name:
APT-1
Short name:
hAPT1
Alternative name(s):
Lysophospholipase 1
Lysophospholipase I
Short name:
LPL-I
Short name:
LysoPLA I
Gene namesi
Name:LYPLA1
Synonyms:APT1, LPL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:6737. LYPLA1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi10434.
OpenTargetsiENSG00000120992.
PharmGKBiPA30499.

Chemistry databases

ChEMBLiCHEMBL1681631.

Polymorphism and mutation databases

BioMutaiLYPLA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022671 – 230Acyl-protein thioesterase 1Add BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO75608.
PaxDbiO75608.
PeptideAtlasiO75608.
PRIDEiO75608.

2D gel databases

OGPiO75608.

PTM databases

iPTMnetiO75608.
PhosphoSitePlusiO75608.
SwissPalmiO75608.

Expressioni

Gene expression databases

BgeeiENSG00000120992.
CleanExiHS_LYPLA1.
ExpressionAtlasiO75608. baseline and differential.
GenevisibleiO75608. HS.

Organism-specific databases

HPAiHPA050941.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi115701. 22 interactors.
IntActiO75608. 3 interactors.
MINTiMINT-5005801.
STRINGi9606.ENSP00000320043.

Chemistry databases

BindingDBiO75608.

Structurei

Secondary structure

1230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 14Combined sources3
Beta strandi21 – 27Combined sources7
Beta strandi30 – 32Combined sources3
Helixi34 – 42Combined sources9
Beta strandi49 – 53Combined sources5
Beta strandi58 – 60Combined sources3
Helixi62 – 64Combined sources3
Beta strandi68 – 70Combined sources3
Helixi86 – 105Combined sources20
Helixi110 – 112Combined sources3
Beta strandi113 – 118Combined sources6
Helixi120 – 129Combined sources10
Beta strandi137 – 143Combined sources7
Helixi149 – 151Combined sources3
Turni160 – 163Combined sources4
Beta strandi166 – 171Combined sources6
Beta strandi175 – 177Combined sources3
Helixi179 – 192Combined sources14
Helixi195 – 197Combined sources3
Beta strandi198 – 203Combined sources6
Helixi212 – 225Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FJ2X-ray1.50A/B6-230[»]
ProteinModelPortaliO75608.
SMRiO75608.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75608.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2112. Eukaryota.
COG0400. LUCA.
GeneTreeiENSGT00390000009648.
HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiO75608.
KOiK06128.
OMAiVEWHEYS.
OrthoDBiEOG091G080L.
PhylomeDBiO75608.
TreeFamiTF314619.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75608-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGNNMSTPL PAIVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIRSSHIK
60 70 80 90 100
YICPHAPVRP VTLNMNVAMP SWFDIIGLSP DSQEDESGIK QAAENIKALI
110 120 130 140 150
DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA
160 170 180 190 200
SFPQGPIGGA NRDISILQCH GDCDPLVPLM FGSLTVEKLK TLVNPANVTF
210 220 230
KTYEGMMHSS CQQEMMDVKQ FIDKLLPPID
Length:230
Mass (Da):24,670
Last modified:November 1, 1998 - v1
Checksum:i90C0522F765F1AC6
GO
Isoform 2 (identifier: O75608-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     57-72: Missing.

Note: No experimental confirmation available.
Show »
Length:214
Mass (Da):22,875
Checksum:i63BCCC806F509CE8
GO

Sequence cautioni

The sequence AAB88180 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127 – 131YTALT → SLIRG in AAB88180 (Ref. 5) Curated5

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_060991153P → S.Corresponds to variant rs11549448dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00919657 – 72Missing in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081281 mRNA. Translation: AAC31610.1.
AF291053 mRNA. Translation: AAG10063.1.
AF077198 mRNA. Translation: AAD26993.1.
AF077199 mRNA. Translation: AAD26994.1.
BC008652 mRNA. Translation: AAH08652.1.
BC010397 mRNA. Translation: AAH10397.1.
AF035293 mRNA. Translation: AAB88180.1. Different initiation.
CCDSiCCDS6157.1. [O75608-1]
CCDS64899.1. [O75608-2]
RefSeqiNP_001266285.1. NM_001279356.1.
NP_001266286.1. NM_001279357.1. [O75608-2]
NP_001266287.1. NM_001279358.1.
NP_001266288.1. NM_001279359.1.
NP_001266289.1. NM_001279360.1.
NP_006321.1. NM_006330.3. [O75608-1]
UniGeneiHs.744046.

Genome annotation databases

EnsembliENST00000316963; ENSP00000320043; ENSG00000120992. [O75608-1]
ENST00000343231; ENSP00000344477; ENSG00000120992. [O75608-2]
GeneIDi10434.
KEGGihsa:10434.
UCSCiuc003xrz.5. human. [O75608-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF081281 mRNA. Translation: AAC31610.1.
AF291053 mRNA. Translation: AAG10063.1.
AF077198 mRNA. Translation: AAD26993.1.
AF077199 mRNA. Translation: AAD26994.1.
BC008652 mRNA. Translation: AAH08652.1.
BC010397 mRNA. Translation: AAH10397.1.
AF035293 mRNA. Translation: AAB88180.1. Different initiation.
CCDSiCCDS6157.1. [O75608-1]
CCDS64899.1. [O75608-2]
RefSeqiNP_001266285.1. NM_001279356.1.
NP_001266286.1. NM_001279357.1. [O75608-2]
NP_001266287.1. NM_001279358.1.
NP_001266288.1. NM_001279359.1.
NP_001266289.1. NM_001279360.1.
NP_006321.1. NM_006330.3. [O75608-1]
UniGeneiHs.744046.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FJ2X-ray1.50A/B6-230[»]
ProteinModelPortaliO75608.
SMRiO75608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115701. 22 interactors.
IntActiO75608. 3 interactors.
MINTiMINT-5005801.
STRINGi9606.ENSP00000320043.

Chemistry databases

BindingDBiO75608.
ChEMBLiCHEMBL1681631.
SwissLipidsiSLP:000000734.

Protein family/group databases

ESTHERihuman-LYPLA1. LYsophospholipase_carboxylesterase.
MEROPSiS09.941.

PTM databases

iPTMnetiO75608.
PhosphoSitePlusiO75608.
SwissPalmiO75608.

Polymorphism and mutation databases

BioMutaiLYPLA1.

2D gel databases

OGPiO75608.

Proteomic databases

EPDiO75608.
PaxDbiO75608.
PeptideAtlasiO75608.
PRIDEiO75608.

Protocols and materials databases

DNASUi10434.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316963; ENSP00000320043; ENSG00000120992. [O75608-1]
ENST00000343231; ENSP00000344477; ENSG00000120992. [O75608-2]
GeneIDi10434.
KEGGihsa:10434.
UCSCiuc003xrz.5. human. [O75608-1]

Organism-specific databases

CTDi10434.
DisGeNETi10434.
GeneCardsiLYPLA1.
HGNCiHGNC:6737. LYPLA1.
HPAiHPA050941.
MIMi605599. gene.
neXtProtiNX_O75608.
OpenTargetsiENSG00000120992.
PharmGKBiPA30499.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2112. Eukaryota.
COG0400. LUCA.
GeneTreeiENSGT00390000009648.
HOGENOMiHOG000260139.
HOVERGENiHBG052378.
InParanoidiO75608.
KOiK06128.
OMAiVEWHEYS.
OrthoDBiEOG091G080L.
PhylomeDBiO75608.
TreeFamiTF314619.

Enzyme and pathway databases

BioCyciZFISH:HS04458-MONOMER.
BRENDAi3.1.2.22. 2681.
ReactomeiR-HSA-203615. eNOS activation.

Miscellaneous databases

ChiTaRSiLYPLA1. human.
EvolutionaryTraceiO75608.
GeneWikiiLYPLA1.
GenomeRNAii10434.
PROiO75608.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120992.
CleanExiHS_LYPLA1.
ExpressionAtlasiO75608. baseline and differential.
GenevisibleiO75608. HS.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR003140. PLipase/COase/thioEstase.
[Graphical view]
PfamiPF02230. Abhydrolase_2. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLYPA1_HUMAN
AccessioniPrimary (citable) accession number: O75608
Secondary accession number(s): O43202, Q9UQF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.