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Reviewed, UniProtKB/Swiss-Prot O75604 (UBP2_HUMAN)

Last modified June 16, 2009. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ubiquitin carboxyl-terminal hydrolase 2
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 2
    Ubiquitin-specific-processing protease 2
    Deubiquitinating enzyme 2
    41 kDa ubiquitin-specific protease
Gene names
Name: USP2
Synonyms: UBP41
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

Subunit structure

Homooligomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

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Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   Molecular functionHydrolase
Protease
Thiol protease
   Technical term3D-structure
Gene Ontology (GO)
   Biological processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from direct assay. Source: HPA

   Molecular functioncysteine-type endopeptidase activity

Traceable author statement. Source: ProtInc

protein binding

Inferred from physical interaction. Source: IntAct

ubiquitin thiolesterase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

KRT15P190121EBI-743272,EBI-739566

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O75604-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O75604-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     253-258: PGRDGM → MLNKAK
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Ubiquitin carboxyl-terminal hydrolase 2
PRO_0000080616

Sites

Active site2761 By similarity
Active site5491 By similarity
Active site5571 By similarity

Natural variations

Alternative sequence1 – 252252Missing in isoform 2.
VSP_005256
Alternative sequence253 – 2586PGRDGM → MLNKAK in isoform 2.
VSP_005257
Natural variant1741R → Q: dbSNP rs33929148.
VAR_051519
Natural variant3831N → S: dbSNP rs45533837.
VAR_051520

Experimental info

Sequence conflict4211S → G in BAB71388. Ref.2
Sequence conflict5011H → R in BAB71388. Ref.2
Sequence conflict5941L → H in AAC28392. Ref.1
Sequence conflict602 – 6054PSRM → TSPI in AAC28392. Ref.1

Secondary structure

1 ..................................................... 605
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: AFF4DA9344D21812

FASTA60568,072
        10         20         30         40         50         60 
MSQLSSTLKR YTESARYTDA HYAKSGYGAY TPSSYGANLA ASLLEKEKLG FKPVPTSSFL 

        70         80         90        100        110        120 
TRPRTYGPSS LLDYDRGRPL LRPDITGGGK RAESQTRGTE RPLGSGLSGG SGFPYGVTNN 

       130        140        150        160        170        180 
CLSYLPINAY DQGVTLTQKL DSQSDLARDF SSLRTSDSYR IDPRNLGRSP MLARTRKELC 

       190        200        210        220        230        240 
TLQGLYQTAS CPEYLVDYLE NYGRKGSASQ VPSQAPPSRV PEIISPTYRP IGRYTLWETG 

       250        260        270        280        290        300 
KGQAPGPSRS SSPGRDGMNS KSAQGLAGLR NLGNTCFMNS ILQCLSNTRE LRDYCLQRLY 

       310        320        330        340        350        360 
MRDLHHGSNA HTALVEEFAK LIQTIWTSSP NDVVSPSEFK TQIQRYAPRF VGYNQQDAQE 

       370        380        390        400        410        420 
FLRFLLDGLH NEVNRVTLRP KSNPENLDHL PDDEKGRQMW RKYLEREDSR IGDLFVGQLK 

       430        440        450        460        470        480 
SSLTCTDCGY CSTVFDPFWD LSLPIAKRGY PEVTLMDCMR LFTKEDVLDG DEKPTCCRCR 

       490        500        510        520        530        540 
GRKRCIKKFS IQRFPKILVL HLKRFSESRI RTSKLTTFVN FPLRDLDLRE FASENTNHAV 

       550        560        570        580        590        600 
YNLYAVSNHS GTTMGGHYTA YCRSPGTGEW HTFNDSSVTP MSSSQVRTSD AYLLFYELAS 


PPSRM

« Hide

Isoform 2.

Checksum: 7CDF2F9A300B864F
Show »

FASTA35340,638

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References

« Hide 'large scale' references
[1]"Cloning and expression of the human and mouse UBP41."
Gong L., Yeh E.T.H.
Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Placenta.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF079564 mRNA. Translation: AAC28392.1.
AK057225 mRNA. Translation: BAB71388.1.
BC002854 mRNA. Translation: AAH02854.1.
BC002955 mRNA. Translation: AAH02955.1.
IPIIPI00216783.
IPI00298910.
RefSeqNP_004196.4.
UniGeneHs.524085

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2HD5X-ray1.85A258-605[»]
2IBIX-ray2.20A251-605[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:29134N.
IntActO75604. 6 interactions.

Protein family/group databases

MEROPSC19.013.

Proteomic databases

PRIDEO75604.

Genome annotation databases

EnsemblENSG00000036672. Homo sapiens. [Contig view]
GeneID9099.
KEGGhsa:9099.

Organism-specific databases

GeneCardsGC11M118731.
HGNCHGNC:12618. USP2.
HPAHPA006777.
HPA007222.
MIM604725. gene.
PharmGKBPA27525.
GenAtlasSearch...

Phylogenomic databases

HOGENOMO75604.
HOVERGENO75604.
OMAO75604. RAESQTR.

Enzyme and pathway databases

BRENDA3.1.2.15. 247.

Gene expression databases

ArrayExpressO75604.
BgeeO75604.
CleanExHS_USP2.
GermOnlineENSG00000036672. Homo sapiens.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio34107.
SOURCESearch...

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Entry information

Entry nameUBP2_HUMAN
AccessionPrimary (citable) accession number: O75604
Secondary accession number(s): Q96MB9, Q9BQ21
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: March 27, 2002
Last modified: June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents