Reviewed, UniProtKB/Swiss-Prot O75592 (MYCB2_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable E3 ubiquitin-protein ligase MYCBP2
    EC=6.3.2.-
Alternative name(s):
    Myc-binding protein 2
    Protein associated with Myc
    Pam/highwire/rpm-1 protein

Gene names

Name:	MYCBP2
Synonyms:	KIAA0916, PAM

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	4640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. May function as a facilitator or regulator of transcriptional activation by MYC. May have a role during synaptogenesis.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with MYC. Interacts with TSC2 (tuberin) when TSC2 is in complex with TSC1 (hamartin). Interacts with FBXO45. Ref.1 Ref.8 Ref.13
Subcellular location	Nucleus. Ref.1
Tissue specificity	Expressed in all tissues examined, expression is exceptionally abundant in brain and thymus. Colocalizes with TSC1 and TSC2 along the neurites and in the growth cones. Colocalized with TSC1 in one of the filopodial extensions at the tip of a growth cone. Ref.1
Sequence similarities	Belongs to the highwire family. Contains 1 B box-type zinc finger. Contains 1 DOC domain. Contains 1 filamin repeat. Contains 5 RCC1 repeats. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAD39842.1 differs from that shown. Reason: Frameshift at position 4636. The sequence CAI39758.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of mitotic metaphase/anaphase transition Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anaphase-promoting complex Inferred from electronic annotation. Source: InterPro
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BAK1	Q16611	1	EBI-1043774,EBI-519866

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: O75592-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75592-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3901-3903: Missing.

Note: Derived from EST data.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 4640	4640	Probable E3 ubiquitin-protein ligase MYCBP2		PRO_0000055963
Regions
	Repeat	562 – 617	56	RCC1 1
	Repeat	661 – 717	57	RCC1 2
	Repeat	869 – 919	51	RCC1 3
	Repeat	920 – 970	51	RCC1 4
	Repeat	972 – 1028	57	RCC1 5
	Repeat	2303 – 2405	103	Filamin
	Domain	3681 – 3859	179	DOC
	Zinc finger	4390 – 4441	52	RING-type; atypical
	Zinc finger	4544 – 4594	51	B box-type
	Compositional bias	65 – 88	24	Lys-rich
	Compositional bias	728 – 776	49	Cys-rich
	Compositional bias	2682 – 2806	125	Ser-rich
	Compositional bias	3156 – 3177	22	Lys-rich
	Compositional bias	3224 – 3255	32	Gly-rich
Amino acid modifications
	Modified residue	140	1	Phosphoserine Ref.12
	Modified residue	143	1	Phosphoserine Ref.12
	Modified residue	145	1	Phosphoserine Ref.12
	Modified residue	1586	1	Phosphoserine By similarity
	Modified residue	1790	1	N6-acetyllysine Ref.15
	Modified residue	2422	1	N6-acetyllysine Ref.15
	Modified residue	2645	1	Phosphothreonine Ref.12
	Modified residue	2749	1	Phosphoserine Ref.10
	Modified residue	2751	1	Phosphoserine Ref.12 Ref.10
	Modified residue	2774	1	Phosphoserine Ref.9
	Modified residue	2831	1	Phosphoserine Ref.10
	Modified residue	3052	1	Phosphoserine Ref.10
	Modified residue	3440	1	Phosphoserine Ref.12
	Modified residue	3467	1	Phosphoserine Ref.12 Ref.11
	Modified residue	3883	1	Phosphothreonine By similarity
	Modified residue	3893	1	Phosphoserine Ref.12 Ref.10
	Modified residue	3894	1	Phosphoserine Ref.12 Ref.10
Natural variations
	Alternative sequence	3901 – 3903	3	Missing in isoform 2.		VSP_014183
	Natural variant	1881	1	A → S: dbSNP rs35887505.		VAR_052086
	Natural variant	2588	1	V → M: dbSNP rs9574002.		VAR_030070
Experimental info
	Sequence conflict	23	1	R → P in AAC39928. Ref.1
	Sequence conflict	202	1	G → V in AAC39928. Ref.1
	Sequence conflict	472	1	G → R in AAC39928. Ref.1
	Sequence conflict	580	1	T → I in AAC39928. Ref.1
	Sequence conflict	865 – 866	2	QL → HV in AAC39928. Ref.1
	Sequence conflict	1474 – 1475	2	GV → PL in AAC39928. Ref.1
	Sequence conflict	2101	1	K → M in AAC39928. Ref.1
	Sequence conflict	3163	1	E → GR in AAC39928. Ref.1
	Sequence conflict	3607	1	A → R in AAC39928. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0557ADF6D26472D6	FASTA	4,640	510,084
	10 20 30 40 50 60 MPVPDGSVAA AGLGLGLPAA DSRGHYQLLL SGRALADRYR RIYTAALNDR DQGGGSAGHP 70 80 90 100 110 120 ASRNKKILNK KKLKRKQKSK SKVKTRSKSE NLENTVIIPD IKLHSNPSAF NIYCNVRHCV 130 140 150 160 170 180 LEWQKKEISL AAASKNSVQS GESDSDEEEE SKEPPIKLPK IIEVGLCEVF ELIKETRFSH 190 200 210 220 230 240 PSLCLRSLQA LLNVLQGQQP EGLQSEPPEV LESLFQLLLE ITVRSTGMND STGQSLTALS 250 260 270 280 290 300 CACLFSLVAS WGETGRTLQA ISAILTNNGS HACQTIQVPT ILNSLQRSVQ AVLVGKIQIQ 310 320 330 340 350 360 DWFSNGIKKA ALMHKWPLKE ISVDEDDQCL LQNDGFFLYL LCKDGLYKIG SGYSGTVRGH 370 380 390 400 410 420 IYNSTSRIRN RKEKKSWLGY AQGYLLYRDV NNHSMTAIRI SPETLEQDGT VMLPDCHTEG 430 440 450 460 470 480 QNILFTDGEY INQIAASRDD GFVVRIFATS TEPVLQQELQ LKLARKCLHA CGISLFDLEK 490 500 510 520 530 540 DLHIISTGFD EESAILGAGR EFALMKTANG KIYYTGKYQS LGIKQGGPSA GKWVELPITK 550 560 570 580 590 600 SPKIVHFSVG HDGSHALLVA EDGSIFFTGS ASKGEDGEST KSRRQSKPYK PKKIIKMEGK 610 620 630 640 650 660 IVVYTACNNG SSSVISKDGE LYMFGKDAIY SDSSSLVTDL KGHFVTQVAM GKAHTCVLMK 670 680 690 700 710 720 NGEVWTFGVN NKGQCGRDTG AMNQGGKGFG VENMATAMDE DLEEELDEKD EKSMMCPPGM 730 740 750 760 770 780 HKWKLEQCMV CTVCGDCTGY GASCVSSGRP DRVPGGICGC GSGESGCAVC GCCKACAREL 790 800 810 820 830 840 DGQEARQRGI LDAVKEMIPL DLLLAVPVPG VNIEEHLQLR QEEKRQRVIR RHRLEEGRGP 850 860 870 880 890 900 LVFAGPIFMN HREQALARLR SHPAQLKHKR DKHKDGSGER GEKDASKITT YPPGSVRFDC 910 920 930 940 950 960 ELRAVQVSCG FHHSVVLMEN GDVYTFGYGQ HGQLGHGDVN SRGCPTLVQA LPGPSTQVTA 970 980 990 1000 1010 1020 GSNHTAVLLM DGQVFTFGSF SKGQLGRPIL DVPYWNAKPA PMPNIGSKYG RKATWIGASG 1030 1040 1050 1060 1070 1080 DQTFLRIDEA LINSHVLATS EIFASKHIIG LVPASISEPP PFKCLLINKV DGSCKTFNDS 1090 1100 1110 1120 1130 1140 EQEDLQGFGV CLDPVYDVIW RFRPNTRELW CYNAVVADAR LPSAADMQSR CSILSPELAL 1150 1160 1170 1180 1190 1200 PTGSRALTTR SHAALHILGC LDTLAAMQDL KMGVASTEEE TQAVMKVYSK EDYSVVNRFE 1210 1220 1230 1240 1250 1260 SHGGGWGYSA HSVEAIRFSA DTDILLGGLG LFGGRGEYTA KIKLFELGPD GGDHETDGDL 1270 1280 1290 1300 1310 1320 LAETDVLAYD CAAREKYAMM FDEPVLLQAG WWYVAWARVS GPSSDCGSHG QASITTDDGV 1330 1340 1350 1360 1370 1380 VFQFKSSKKS NNGTDVNAGQ IPQLLYRLPT SDGSASKGKQ QTSEPVHILK RSFARTVSVE 1390 1400 1410 1420 1430 1440 CFESLLSILH WSWTTLVLGV EELRGLKGFQ FTATLLDLER LRFVGTCCLR LLRVYTCEIY 1450 1460 1470 1480 1490 1500 PVSATGKAVV EETSKLAECI GKTRTLLRKI LSEGVDHCMV KLDNDPQGYL SQPLSLLEAV 1510 1520 1530 1540 1550 1560 LQECHNTFTA CFHSFYPTPA LQWACLCDLL NCLDQDIQEA NFKTSSSRLL AAVMSALCHT 1570 1580 1590 1600 1610 1620 SVKLTSIFPI AYDGEVLLRS IVKQVSTEND STLVHRFPLL VAHMEKLSQS EENISGMTSF 1630 1640 1650 1660 1670 1680 REVLEKMLVI VVLPVRNSLR RENELFSSHL VSNTCGLLAS IVSELTASAL GSEVDGLNSL 1690 1700 1710 1720 1730 1740 HSVKASANRF TKTSQGRSWN TGNGSPDAIC FSVDKPGIVV VGFSVYGGGG IHEYELEVLV 1750 1760 1770 1780 1790 1800 DDSEHAGDST HSHRWTSLEL VKGTYTTDDS PSDIAEIRLD KVVPLKENVK YAVRLRNYGS 1810 1820 1830 1840 1850 1860 RTANGDGGMT TVQCPDGVTF TFSTCSLSSN GTNQTRGQIP QILYYRSEFD GDLQSQLLSK 1870 1880 1890 1900 1910 1920 ANEEDKNCSR ALSVVSTVVR ASKDLLHRAL AVDADDIPEL LSSSSLFSML LPLIIAYIGP 1930 1940 1950 1960 1970 1980 VAAAIPKVAV EVFGLVQQLL PSVAILNQKY APPAFNPNQS TDSTTGNQPE QGLSACTTSS 1990 2000 2010 2020 2030 2040 HYAVIESEHP YKPACVMHYK VTFPECVRWM TIEFDPQCGT AQSEDVLRLL IPVRTVQNSG 2050 2060 2070 2080 2090 2100 YGPKLTSVHE NLNSWIELKK FSGSSGWPTM VLVLPGNEAL FSLETASDYV KDDKASFYGF 2110 2120 2130 2140 2150 2160 KCFAIGYEFS PGPDEGVIQL EKELANLGGV CAAALMKKDL ALPIGNELEE DLEILEEAAL 2170 2180 2190 2200 2210 2220 QVCKTHSGIL GKGLALSHSP TILEALEGNL PLQIQSNEQS FLDDFIACVP GSSGGRLARW 2230 2240 2250 2260 2270 2280 LQPDSYADPQ KTSLILNKDD IRCGWPTTIT VQTKDQYGDV VHVPNMKVEV KAVPVSQKKM 2290 2300 2310 2320 2330 2340 SLQQDQAKKP QRIPGSPAVT AASSNTDMTY GGLASPKLDV SYEPMIVKEA RYIAITMMKV 2350 2360 2370 2380 2390 2400 YENYSFEELR FASPTPKRPS ENMLIRVNND GTYCANWTPG AIGLYTLHVT IDGIEIDAGL 2410 2420 2430 2440 2450 2460 EVKVKDPPKG MIPPGTQLVK PKSEPQPNKV RKFVAKDSAG LRIRSHPSLQ SEQIGIVKVN 2470 2480 2490 2500 2510 2520 GTITFIDEIH NDDGVWLRLN DETIKKYVPN MNGYTEAWCL SFNQHLGKSL LVPVDESKTN 2530 2540 2550 2560 2570 2580 TDDFFKDINS CCPQEATMQE QDMPFLRGGP GMYKVVKTGP SGHNIRSCPN LRGIPIGMLV 2590 2600 2610 2620 2630 2640 LGNKVKAVGE VTNSEGTWVQ LDQNSMVEFC ESDEGEAWSL ARDRGGNQYL RHEDEQALLD 2650 2660 2670 2680 2690 2700 QNSQTPPPSP FSVQAFNKGA SCSAQGFDYG LGNSKGDRGN ISTSSKPAST SGKSELSSKH 2710 2720 2730 2740 2750 2760 SRSLKPDGRM SRTTADQKKP RGTESLSASE SLILKSDAAK LRSDSHSRSL SPNHNTLQTL 2770 2780 2790 2800 2810 2820 KSDGRMPSSS RAESPGPGSR LSSPKPKTLP ANRSSPSGAS SPRSSSPHDK NLPQKSTAPV 2830 2840 2850 2860 2870 2880 KTKLDPPRER SKSDSYTLDP DTLRKKKMPL TEPLRGRSTS PKPKSVPKDS TDSPGSENRA 2890 2900 2910 2920 2930 2940 PSPHVVQENL HSEVVEVCTS STLKTNSLTD STCDDSSEFK SVDEGSNKVH FSIGKAPLKD 2950 2960 2970 2980 2990 3000 EQEMRASPKI SRKCANRHTR PKKEKSSFLF KGDGSKPLEP AKQAMSPSVA ECARAVFASF 3010 3020 3030 3040 3050 3060 LWHEGIVHDA MACSSFLKFH PELSKEHAPI RSSLNSQQPT EEKETKLKNR HSLEISSALN 3070 3080 3090 3100 3110 3120 MFNIAPHGPD ISKMGSINKN KVLSMLKEPP LHEKCEDGKT ETTFEMSMHN TMKSKSPLPL 3130 3140 3150 3160 3170 3180 TLQHLVAFWE DISLATIKAA SQNMIFPSPG SCAVLKKKEC EKENKKSKKE KKKKEKAEVR 3190 3200 3210 3220 3230 3240 PRGNLFGEMA QLAVGGPEKD TICELCGESH PYPVTYHMRQ AHPGCGRYAG GQGYNSIGHF 3250 3260 3270 3280 3290 3300 CGGWAGNCGD GGIGGSTWYL VCDRCREKYL REKQAAAREK VKQSRRKPMQ VKTPRALPTM 3310 3320 3330 3340 3350 3360 EAHQVIKANA LFLLSLSSAA EPSILCYHPA KPFQSQLPSV KEGISEDLPV KMPCLYLQTL 3370 3380 3390 3400 3410 3420 ARHHHENFVG YQDDNLFQDE MRYLRSTSVP APYISVTPDA SPNVFEEPES NMKSMPPSLE 3430 3440 3450 3460 3470 3480 TSPITDTDLA KRTVFQRSYS VVASEYDKQH SILPARVKAI PRRRVNSGDT EVGSSLLRHP 3490 3500 3510 3520 3530 3540 SPELSRLISA HSSLSKGERN FQWPVLAFVI QHHDLEGLEI AMKQALRKSA CRVFAMEAFN 3550 3560 3570 3580 3590 3600 WLLCNVIQTT SLHDILWHFV ASLTPAPVEP EEEEDEENKT SKENSEQEKD TRVCEHPLSD 3610 3620 3630 3640 3650 3660 IVIAGEAAHP LPHTFHRLLQ TISDLMMSLP SGSSLQQMAL RCWSLKFKQS DHQFLHQSNV 3670 3680 3690 3700 3710 3720 FHHINNILSK SDDGDSEESF SISIQSGFEA MSQELCIVMC LKDLTSIVDI KTSSRPAMIG 3730 3740 3750 3760 3770 3780 SLTDGSTETF WESGDEDKNK TKNITINCVK GINARYVSVH VDNSRDLGNK VTSMTFLTGK 3790 3800 3810 3820 3830 3840 AVEDLCRIKQ VDLDSRHIGW VTSELPGGDN HIIKIELKGP ENTLRVRQVK VLGWKDGEST 3850 3860 3870 3880 3890 3900 KIAGQISASV AQQRNCEAET LRVFRLITSQ VFGKLISGDA EPTPEQEEKA LLSSPEGEEK 3910 3920 3930 3940 3950 3960 VYNATSDADL KEHMVGIIFS RSKLTNLQKQ VCAHIVQAIR MEATRVREEW EHAISSKENA 3970 3980 3990 4000 4010 4020 NSQPNDEDAS SDAYCFELLS MVLALSGSNV GRQYLAQQLT LLQDLFSLLH TASPRVQRQV 4030 4040 4050 4060 4070 4080 TSLLRRVLPE VTPSRLASII GVKSLPPADI SDIIHSTEKG DWNKLGILDM FLGCIAKALT 4090 4100 4110 4120 4130 4140 VQLKAKGTTI TGTAGTTVGK GVTTVTLPMI FNSSYLRRGE SHWWMKGSTP TQISEIIIKL 4150 4160 4170 4180 4190 4200 IKDMAAGHLS EAWSRVTKNA IAETIIALTK MEEEFRSPVR CIATTRLWLA LASLCVLDQD 4210 4220 4230 4240 4250 4260 HVDRLSSGRW MGKDGQQKQM PMCDNHDDGE TAAIILCNVC GNLCTDCDRF LHLHRRTKTH 4270 4280 4290 4300 4310 4320 QRQVFKEEEE AIKVDLHEGC GRTKLFWLMA LADSKTMKAM VEFREHTGKP TTSSSEACRF 4330 4340 4350 4360 4370 4380 CGSRSGTELS AVGSVCSDAD CQEYAKIACS KTHPCGHPCG GVKNEEHCLP CLHGCDKSAT 4390 4400 4410 4420 4430 4440 SLKQDADDMC MICFTEALSA APAIQLDCSH IFHLQCCRRV LENRWLGPRI TFGFISCPIC 4450 4460 4470 4480 4490 4500 KNKINHIVLK DLLDPIKELY EDVRRKALMR LEYEGLHKSE AITTPGVRFY NDPAGYAMNR 4510 4520 4530 4540 4550 4560 YAYYVCYKCR KAYFGGEARC DAEAGRGDDY DPRELICGAC SDVSRAQMCP KHGTDFLEYK 4570 4580 4590 4600 4610 4620 CRYCCSVAVF FCFGTTHFCN ACHDDFQRMT SIPKEELPHC PAGPKGKQLE GTECPLHVVH 4630 4640 PPTGEEFALG CGVCRNAHTF
	Isoform 2. Checksum: A54457A00B327ADA	FASTA	4,637	509,708
	10 20 30 40 50 60 MPVPDGSVAA AGLGLGLPAA DSRGHYQLLL SGRALADRYR RIYTAALNDR DQGGGSAGHP 70 80 90 100 110 120 ASRNKKILNK KKLKRKQKSK SKVKTRSKSE NLENTVIIPD IKLHSNPSAF NIYCNVRHCV 130 140 150 160 170 180 LEWQKKEISL AAASKNSVQS GESDSDEEEE SKEPPIKLPK IIEVGLCEVF ELIKETRFSH 190 200 210 220 230 240 PSLCLRSLQA LLNVLQGQQP EGLQSEPPEV LESLFQLLLE ITVRSTGMND STGQSLTALS 250 260 270 280 290 300 CACLFSLVAS WGETGRTLQA ISAILTNNGS HACQTIQVPT ILNSLQRSVQ AVLVGKIQIQ 310 320 330 340 350 360 DWFSNGIKKA ALMHKWPLKE ISVDEDDQCL LQNDGFFLYL LCKDGLYKIG SGYSGTVRGH 370 380 390 400 410 420 IYNSTSRIRN RKEKKSWLGY AQGYLLYRDV NNHSMTAIRI SPETLEQDGT VMLPDCHTEG 430 440 450 460 470 480 QNILFTDGEY INQIAASRDD GFVVRIFATS TEPVLQQELQ LKLARKCLHA CGISLFDLEK 490 500 510 520 530 540 DLHIISTGFD EESAILGAGR EFALMKTANG KIYYTGKYQS LGIKQGGPSA GKWVELPITK 550 560 570 580 590 600 SPKIVHFSVG HDGSHALLVA EDGSIFFTGS ASKGEDGEST KSRRQSKPYK PKKIIKMEGK 610 620 630 640 650 660 IVVYTACNNG SSSVISKDGE LYMFGKDAIY SDSSSLVTDL KGHFVTQVAM GKAHTCVLMK 670 680 690 700 710 720 NGEVWTFGVN NKGQCGRDTG AMNQGGKGFG VENMATAMDE DLEEELDEKD EKSMMCPPGM 730 740 750 760 770 780 HKWKLEQCMV CTVCGDCTGY GASCVSSGRP DRVPGGICGC GSGESGCAVC GCCKACAREL 790 800 810 820 830 840 DGQEARQRGI LDAVKEMIPL DLLLAVPVPG VNIEEHLQLR QEEKRQRVIR RHRLEEGRGP 850 860 870 880 890 900 LVFAGPIFMN HREQALARLR SHPAQLKHKR DKHKDGSGER GEKDASKITT YPPGSVRFDC 910 920 930 940 950 960 ELRAVQVSCG FHHSVVLMEN GDVYTFGYGQ HGQLGHGDVN SRGCPTLVQA LPGPSTQVTA 970 980 990 1000 1010 1020 GSNHTAVLLM DGQVFTFGSF SKGQLGRPIL DVPYWNAKPA PMPNIGSKYG RKATWIGASG 1030 1040 1050 1060 1070 1080 DQTFLRIDEA LINSHVLATS EIFASKHIIG LVPASISEPP PFKCLLINKV DGSCKTFNDS 1090 1100 1110 1120 1130 1140 EQEDLQGFGV CLDPVYDVIW RFRPNTRELW CYNAVVADAR LPSAADMQSR CSILSPELAL 1150 1160 1170 1180 1190 1200 PTGSRALTTR SHAALHILGC LDTLAAMQDL KMGVASTEEE TQAVMKVYSK EDYSVVNRFE 1210 1220 1230 1240 1250 1260 SHGGGWGYSA HSVEAIRFSA DTDILLGGLG LFGGRGEYTA KIKLFELGPD GGDHETDGDL 1270 1280 1290 1300 1310 1320 LAETDVLAYD CAAREKYAMM FDEPVLLQAG WWYVAWARVS GPSSDCGSHG QASITTDDGV 1330 1340 1350 1360 1370 1380 VFQFKSSKKS NNGTDVNAGQ IPQLLYRLPT SDGSASKGKQ QTSEPVHILK RSFARTVSVE 1390 1400 1410 1420 1430 1440 CFESLLSILH WSWTTLVLGV EELRGLKGFQ FTATLLDLER LRFVGTCCLR LLRVYTCEIY 1450 1460 1470 1480 1490 1500 PVSATGKAVV EETSKLAECI GKTRTLLRKI LSEGVDHCMV KLDNDPQGYL SQPLSLLEAV 1510 1520 1530 1540 1550 1560 LQECHNTFTA CFHSFYPTPA LQWACLCDLL NCLDQDIQEA NFKTSSSRLL AAVMSALCHT 1570 1580 1590 1600 1610 1620 SVKLTSIFPI AYDGEVLLRS IVKQVSTEND STLVHRFPLL VAHMEKLSQS EENISGMTSF 1630 1640 1650 1660 1670 1680 REVLEKMLVI VVLPVRNSLR RENELFSSHL VSNTCGLLAS IVSELTASAL GSEVDGLNSL 1690 1700 1710 1720 1730 1740 HSVKASANRF TKTSQGRSWN TGNGSPDAIC FSVDKPGIVV VGFSVYGGGG IHEYELEVLV 1750 1760 1770 1780 1790 1800 DDSEHAGDST HSHRWTSLEL VKGTYTTDDS PSDIAEIRLD KVVPLKENVK YAVRLRNYGS 1810 1820 1830 1840 1850 1860 RTANGDGGMT TVQCPDGVTF TFSTCSLSSN GTNQTRGQIP QILYYRSEFD GDLQSQLLSK 1870 1880 1890 1900 1910 1920 ANEEDKNCSR ALSVVSTVVR ASKDLLHRAL AVDADDIPEL LSSSSLFSML LPLIIAYIGP 1930 1940 1950 1960 1970 1980 VAAAIPKVAV EVFGLVQQLL PSVAILNQKY APPAFNPNQS TDSTTGNQPE QGLSACTTSS 1990 2000 2010 2020 2030 2040 HYAVIESEHP YKPACVMHYK VTFPECVRWM TIEFDPQCGT AQSEDVLRLL IPVRTVQNSG 2050 2060 2070 2080 2090 2100 YGPKLTSVHE NLNSWIELKK FSGSSGWPTM VLVLPGNEAL FSLETASDYV KDDKASFYGF 2110 2120 2130 2140 2150 2160 KCFAIGYEFS PGPDEGVIQL EKELANLGGV CAAALMKKDL ALPIGNELEE DLEILEEAAL 2170 2180 2190 2200 2210 2220 QVCKTHSGIL GKGLALSHSP TILEALEGNL PLQIQSNEQS FLDDFIACVP GSSGGRLARW 2230 2240 2250 2260 2270 2280 LQPDSYADPQ KTSLILNKDD IRCGWPTTIT VQTKDQYGDV VHVPNMKVEV KAVPVSQKKM 2290 2300 2310 2320 2330 2340 SLQQDQAKKP QRIPGSPAVT AASSNTDMTY GGLASPKLDV SYEPMIVKEA RYIAITMMKV 2350 2360 2370 2380 2390 2400 YENYSFEELR FASPTPKRPS ENMLIRVNND GTYCANWTPG AIGLYTLHVT IDGIEIDAGL 2410 2420 2430 2440 2450 2460 EVKVKDPPKG MIPPGTQLVK PKSEPQPNKV RKFVAKDSAG LRIRSHPSLQ SEQIGIVKVN 2470 2480 2490 2500 2510 2520 GTITFIDEIH NDDGVWLRLN DETIKKYVPN MNGYTEAWCL SFNQHLGKSL LVPVDESKTN 2530 2540 2550 2560 2570 2580 TDDFFKDINS CCPQEATMQE QDMPFLRGGP GMYKVVKTGP SGHNIRSCPN LRGIPIGMLV 2590 2600 2610 2620 2630 2640 LGNKVKAVGE VTNSEGTWVQ LDQNSMVEFC ESDEGEAWSL ARDRGGNQYL RHEDEQALLD 2650 2660 2670 2680 2690 2700 QNSQTPPPSP FSVQAFNKGA SCSAQGFDYG LGNSKGDRGN ISTSSKPAST SGKSELSSKH 2710 2720 2730 2740 2750 2760 SRSLKPDGRM SRTTADQKKP RGTESLSASE SLILKSDAAK LRSDSHSRSL SPNHNTLQTL 2770 2780 2790 2800 2810 2820 KSDGRMPSSS RAESPGPGSR LSSPKPKTLP ANRSSPSGAS SPRSSSPHDK NLPQKSTAPV 2830 2840 2850 2860 2870 2880 KTKLDPPRER SKSDSYTLDP DTLRKKKMPL TEPLRGRSTS PKPKSVPKDS TDSPGSENRA 2890 2900 2910 2920 2930 2940 PSPHVVQENL HSEVVEVCTS STLKTNSLTD STCDDSSEFK SVDEGSNKVH FSIGKAPLKD 2950 2960 2970 2980 2990 3000 EQEMRASPKI SRKCANRHTR PKKEKSSFLF KGDGSKPLEP AKQAMSPSVA ECARAVFASF 3010 3020 3030 3040 3050 3060 LWHEGIVHDA MACSSFLKFH PELSKEHAPI RSSLNSQQPT EEKETKLKNR HSLEISSALN 3070 3080 3090 3100 3110 3120 MFNIAPHGPD ISKMGSINKN KVLSMLKEPP LHEKCEDGKT ETTFEMSMHN TMKSKSPLPL 3130 3140 3150 3160 3170 3180 TLQHLVAFWE DISLATIKAA SQNMIFPSPG SCAVLKKKEC EKENKKSKKE KKKKEKAEVR 3190 3200 3210 3220 3230 3240 PRGNLFGEMA QLAVGGPEKD TICELCGESH PYPVTYHMRQ AHPGCGRYAG GQGYNSIGHF 3250 3260 3270 3280 3290 3300 CGGWAGNCGD GGIGGSTWYL VCDRCREKYL REKQAAAREK VKQSRRKPMQ VKTPRALPTM 3310 3320 3330 3340 3350 3360 EAHQVIKANA LFLLSLSSAA EPSILCYHPA KPFQSQLPSV KEGISEDLPV KMPCLYLQTL 3370 3380 3390 3400 3410 3420 ARHHHENFVG YQDDNLFQDE MRYLRSTSVP APYISVTPDA SPNVFEEPES NMKSMPPSLE 3430 3440 3450 3460 3470 3480 TSPITDTDLA KRTVFQRSYS VVASEYDKQH SILPARVKAI PRRRVNSGDT EVGSSLLRHP 3490 3500 3510 3520 3530 3540 SPELSRLISA HSSLSKGERN FQWPVLAFVI QHHDLEGLEI AMKQALRKSA CRVFAMEAFN 3550 3560 3570 3580 3590 3600 WLLCNVIQTT SLHDILWHFV ASLTPAPVEP EEEEDEENKT SKENSEQEKD TRVCEHPLSD 3610 3620 3630 3640 3650 3660 IVIAGEAAHP LPHTFHRLLQ TISDLMMSLP SGSSLQQMAL RCWSLKFKQS DHQFLHQSNV 3670 3680 3690 3700 3710 3720 FHHINNILSK SDDGDSEESF SISIQSGFEA MSQELCIVMC LKDLTSIVDI KTSSRPAMIG 3730 3740 3750 3760 3770 3780 SLTDGSTETF WESGDEDKNK TKNITINCVK GINARYVSVH VDNSRDLGNK VTSMTFLTGK 3790 3800 3810 3820 3830 3840 AVEDLCRIKQ VDLDSRHIGW VTSELPGGDN HIIKIELKGP ENTLRVRQVK VLGWKDGEST 3850 3860 3870 3880 3890 3900 KIAGQISASV AQQRNCEAET LRVFRLITSQ VFGKLISGDA EPTPEQEEKA LLSSPEGEEK 3910 3920 3930 3940 3950 3960 ATSDADLKEH MVGIIFSRSK LTNLQKQVCA HIVQAIRMEA TRVREEWEHA ISSKENANSQ 3970 3980 3990 4000 4010 4020 PNDEDASSDA YCFELLSMVL ALSGSNVGRQ YLAQQLTLLQ DLFSLLHTAS PRVQRQVTSL 4030 4040 4050 4060 4070 4080 LRRVLPEVTP SRLASIIGVK SLPPADISDI IHSTEKGDWN KLGILDMFLG CIAKALTVQL 4090 4100 4110 4120 4130 4140 KAKGTTITGT AGTTVGKGVT TVTLPMIFNS SYLRRGESHW WMKGSTPTQI SEIIIKLIKD 4150 4160 4170 4180 4190 4200 MAAGHLSEAW SRVTKNAIAE TIIALTKMEE EFRSPVRCIA TTRLWLALAS LCVLDQDHVD 4210 4220 4230 4240 4250 4260 RLSSGRWMGK DGQQKQMPMC DNHDDGETAA IILCNVCGNL CTDCDRFLHL HRRTKTHQRQ 4270 4280 4290 4300 4310 4320 VFKEEEEAIK VDLHEGCGRT KLFWLMALAD SKTMKAMVEF REHTGKPTTS SSEACRFCGS 4330 4340 4350 4360 4370 4380 RSGTELSAVG SVCSDADCQE YAKIACSKTH PCGHPCGGVK NEEHCLPCLH GCDKSATSLK 4390 4400 4410 4420 4430 4440 QDADDMCMIC FTEALSAAPA IQLDCSHIFH LQCCRRVLEN RWLGPRITFG FISCPICKNK 4450 4460 4470 4480 4490 4500 INHIVLKDLL DPIKELYEDV RRKALMRLEY EGLHKSEAIT TPGVRFYNDP AGYAMNRYAY 4510 4520 4530 4540 4550 4560 YVCYKCRKAY FGGEARCDAE AGRGDDYDPR ELICGACSDV SRAQMCPKHG TDFLEYKCRY 4570 4580 4590 4600 4610 4620 CCSVAVFFCF GTTHFCNACH DDFQRMTSIP KEELPHCPAG PKGKQLEGTE CPLHVVHPPT 4630 GEEFALGCGV CRNAHTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a large Myc-binding protein that contains RCC1-like repeats." Guo Q., Xie J., Dang C.V., Liu E.T., Bishop J.M. Proc. Natl. Acad. Sci. U.S.A. 95:9172-9177(1998) [PubMed: 9689053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYC, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3431-4640 (ISOFORM 1). Tissue: Brain.
[5]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3789-4640 (ISOFORM 1). Tissue: Brain.
[7]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4367-4640. Tissue: Umbilical cord blood.
[8]	"Pam and its ortholog highwire interact with and may negatively regulate the TSC1.TSC2 complex." Murthy V., Han S., Beauchamp R.L., Smith N., Haddad L.A., Ito N., Ramesh V. J. Biol. Chem. 279:1351-1358(2004) [PubMed: 14559897] [Abstract] Cited for: INTERACTION WITH TSC2.
[9]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2774, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2749; SER-2751; SER-2831; SER-3052; SER-3893 AND SER-3894, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3467, MASS SPECTROMETRY.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-143; SER-145; THR-2645; SER-2751; SER-3440; SER-3467; SER-3893 AND SER-3894, MASS SPECTROMETRY.
[13]	"Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development." Saiga T., Fukuda T., Matsumoto M., Tada H., Okano H.J., Okano H., Nakayama K.I. Mol. Cell. Biol. 29:3529-3543(2009) [PubMed: 19398581] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH FBXO45.
[14]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[15]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-1790 AND LYS-2422, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF075587 mRNA. Translation: AAC39928.1. AL159154 , AL159158, AL359257, AC001226 Genomic DNA. Translation: CAH73736.1. AL159158 , AL159154, AL359257, AC001226 Genomic DNA. Translation: CAI16842.1. AL359257 , AL159158, AL159154, AC001226 Genomic DNA. Translation: CAI39759.1. AL359257, AC001226 Genomic DNA. Translation: CAI39758.1. Sequence problems. CH471093 Genomic DNA. Translation: EAW80565.1. AB020723 mRNA. Translation: BAA74939.1. BC037971 mRNA. Translation: AAH37971.1. Different initiation. AF083244 mRNA. Translation: AAD39842.1. Sequence problems.
IPI	IPI00289776. IPI00607852.
RefSeq	NP_055872.3.
UniGene	Hs.591221
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:28142N.
IntAct	O75592. 3 interactions.
STRING	O75592.
PTM databases
PhosphoSite	O75592.
Proteomic databases
PRIDE	O75592.
Genome annotation databases
Ensembl	ENST00000357337; ENSP00000349892; ENSG00000005810; Homo sapiens. [Genome view] ENST00000360084; ENSP00000353197; ENSG00000005810; Homo sapiens. [Genome view] ENST00000407578; ENSP00000384288; ENSG00000005810; Homo sapiens. [Genome view] ENST00000429715; ENSP00000413907; ENSG00000005810; Homo sapiens. [Genome view]
GeneID	23077.
KEGG	hsa:23077.
UCSC	uc001vkf.1. human.
Organism-specific databases
CTD	23077.
GeneCards	GC13M076517.
HGNC	HGNC:23386. MYCBP2.
MIM	610392. gene.
PharmGKB	PA134871126.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75592.
HOVERGEN	O75592.
OMA	IPKVAVE.
Gene expression databases
ArrayExpress	O75592.
Bgee	O75592.
CleanEx	HS_MYCBP2. HS_PAM.
Genevestigator	O75592.
GermOnline	ENSG00000005810. Homo sapiens.
Family and domain databases
InterPro	IPR004939. APC10. IPR017868. Filamin/ABP280_repeat-like. IPR012983. PHR. IPR000408. Reg_chr_condens. IPR009091. Reg_csome_cond/b-lactamase_inh. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Gene3D	G3DSA:2.130.10.30. Reg_csome_cond/b-lactamase_inh. 1 hit.
Pfam	PF08005. PHR. 2 hits. PF00415. RCC1. 1 hit. [Graphical view]
PRINTS	PR00633. RCCNDNSATION.
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS51284. DOC. 1 hit. PS50194. FILAMIN_REPEAT. 1 hit. PS00625. RCC1_1. False negative. PS00626. RCC1_2. 2 hits. PS50012. RCC1_3. 3 hits. PS50119. ZF_BBOX. False negative. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44191.
SOURCE	Search...

Entry information

Entry name

MYCB2_HUMAN

Accession

Primary (citable) accession number: O75592
Secondary accession number(s): A6NJC6 , Q5JSX8, Q5VZN6, Q6PIB6, Q9UQ11, Q9Y6E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.