Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O75592 (MYCB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase MYCBP2

EC=6.3.2.-
Alternative name(s):
Myc-binding protein 2
Pam/highwire/rpm-1 protein
Protein associated with Myc
Gene names
Name:MYCBP2
Synonyms:KIAA0916, PAM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length4640 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. Interacts with the E2 enzymes UBE2D1, UBE2D3 and UBE2L3 (in vitro). May function as a facilitator or regulator of transcriptional activation by MYC. May have a role during synaptogenesis. Ref.10

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with MYC. Interacts with TSC2 (tuberin) when TSC2 is in complex with TSC1 (hamartin). Interacts with FBXO45. Ref.1 Ref.8 Ref.14

Subcellular location

Nucleus Ref.1.

Tissue specificity

Expressed in all tissues examined, expression is exceptionally abundant in brain and thymus. Colocalizes with TSC1 and TSC2 along the neurites and in the growth cones. Colocalized with TSC1 in one of the filopodial extensions at the tip of a growth cone. Ref.1

Domain

The PHR domains are compact beta-sandwich folds composed of 11 antiparallel strands and decorated with conserved apical loops. They are likely to play a structural role and mediate interactions with substrates or partners By similarity.

Post-translational modification

Autoubiquitinated.

Sequence similarities

Belongs to the highwire family.

Contains 1 B box-type zinc finger.

Contains 1 DOC domain.

Contains 1 filamin repeat.

Contains 5 RCC1 repeats.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence AAD39842.1 differs from that shown. Reason: Frameshift at position 4636.

The sequence AAH37971.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI39758.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
Ubl conjugation pathway
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionLigase
   PTMDisulfide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbranchiomotor neuron axon guidance

Inferred from electronic annotation. Source: Ensembl

central nervous system projection neuron axonogenesis

Inferred from electronic annotation. Source: Ensembl

regulation of cytoskeleton organization

Inferred from electronic annotation. Source: Ensembl

regulation of protein localization

Inferred from electronic annotation. Source: Ensembl

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentaxon

Inferred from electronic annotation. Source: Ensembl

microtubule cytoskeleton

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 22357847PubMed 24136289. Source: IntAct

ubiquitin-protein transferase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Q99IB83EBI-1043774,EBI-6927928From a different organism.
Rae1Q3SWS82EBI-1043774,EBI-6920222From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: O75592-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O75592-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3901-3903: Missing.
Note: Derived from EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 46404640E3 ubiquitin-protein ligase MYCBP2
PRO_0000055963

Regions

Repeat562 – 61756RCC1 1
Repeat661 – 71757RCC1 2
Repeat869 – 91951RCC1 3
Repeat920 – 97051RCC1 4
Repeat972 – 102857RCC1 5
Repeat2303 – 2405103Filamin
Domain3681 – 3859179DOC
Zinc finger4390 – 444152RING-type; atypical
Zinc finger4544 – 459451B box-type
Region1197 – 1348152PHR domain 1 By similarity
Region1688 – 1846159PHR domain 2 By similarity
Compositional bias65 – 8824Lys-rich
Compositional bias728 – 77649Cys-rich
Compositional bias2682 – 2806125Ser-rich
Compositional bias3156 – 317722Lys-rich
Compositional bias3224 – 325532Gly-rich

Amino acid modifications

Modified residue1401Phosphoserine Ref.12
Modified residue1431Phosphoserine Ref.12
Modified residue1451Phosphoserine Ref.12
Modified residue26451Phosphothreonine Ref.12 Ref.16 Ref.17
Modified residue27511Phosphoserine Ref.12
Modified residue28821Phosphoserine Ref.16
Modified residue30521Phosphoserine Ref.9
Modified residue34401Phosphoserine Ref.12
Modified residue34671Phosphoserine Ref.11 Ref.12 Ref.15 Ref.16 Ref.17
Disulfide bond1710 ↔ 1825 By similarity

Natural variations

Alternative sequence3901 – 39033Missing in isoform 2.
VSP_014183
Natural variant18811A → S.
Corresponds to variant rs35887505 [ dbSNP | Ensembl ].
VAR_052086
Natural variant25881V → M.
Corresponds to variant rs9574002 [ dbSNP | Ensembl ].
VAR_030070

Experimental info

Sequence conflict231R → P in AAC39928. Ref.1
Sequence conflict2021G → V in AAC39928. Ref.1
Sequence conflict4721G → R in AAC39928. Ref.1
Sequence conflict5801T → I in AAC39928. Ref.1
Sequence conflict865 – 8662QL → HV in AAC39928. Ref.1
Sequence conflict1474 – 14752GV → PL in AAC39928. Ref.1
Sequence conflict21011K → M in AAC39928. Ref.1
Sequence conflict31631E → GR in AAC39928. Ref.1
Sequence conflict36071A → R in AAC39928. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 0557ADF6D26472D6

FASTA4,640510,084
        10         20         30         40         50         60 
MPVPDGSVAA AGLGLGLPAA DSRGHYQLLL SGRALADRYR RIYTAALNDR DQGGGSAGHP 

        70         80         90        100        110        120 
ASRNKKILNK KKLKRKQKSK SKVKTRSKSE NLENTVIIPD IKLHSNPSAF NIYCNVRHCV 

       130        140        150        160        170        180 
LEWQKKEISL AAASKNSVQS GESDSDEEEE SKEPPIKLPK IIEVGLCEVF ELIKETRFSH 

       190        200        210        220        230        240 
PSLCLRSLQA LLNVLQGQQP EGLQSEPPEV LESLFQLLLE ITVRSTGMND STGQSLTALS 

       250        260        270        280        290        300 
CACLFSLVAS WGETGRTLQA ISAILTNNGS HACQTIQVPT ILNSLQRSVQ AVLVGKIQIQ 

       310        320        330        340        350        360 
DWFSNGIKKA ALMHKWPLKE ISVDEDDQCL LQNDGFFLYL LCKDGLYKIG SGYSGTVRGH 

       370        380        390        400        410        420 
IYNSTSRIRN RKEKKSWLGY AQGYLLYRDV NNHSMTAIRI SPETLEQDGT VMLPDCHTEG 

       430        440        450        460        470        480 
QNILFTDGEY INQIAASRDD GFVVRIFATS TEPVLQQELQ LKLARKCLHA CGISLFDLEK 

       490        500        510        520        530        540 
DLHIISTGFD EESAILGAGR EFALMKTANG KIYYTGKYQS LGIKQGGPSA GKWVELPITK 

       550        560        570        580        590        600 
SPKIVHFSVG HDGSHALLVA EDGSIFFTGS ASKGEDGEST KSRRQSKPYK PKKIIKMEGK 

       610        620        630        640        650        660 
IVVYTACNNG SSSVISKDGE LYMFGKDAIY SDSSSLVTDL KGHFVTQVAM GKAHTCVLMK 

       670        680        690        700        710        720 
NGEVWTFGVN NKGQCGRDTG AMNQGGKGFG VENMATAMDE DLEEELDEKD EKSMMCPPGM 

       730        740        750        760        770        780 
HKWKLEQCMV CTVCGDCTGY GASCVSSGRP DRVPGGICGC GSGESGCAVC GCCKACAREL 

       790        800        810        820        830        840 
DGQEARQRGI LDAVKEMIPL DLLLAVPVPG VNIEEHLQLR QEEKRQRVIR RHRLEEGRGP 

       850        860        870        880        890        900 
LVFAGPIFMN HREQALARLR SHPAQLKHKR DKHKDGSGER GEKDASKITT YPPGSVRFDC 

       910        920        930        940        950        960 
ELRAVQVSCG FHHSVVLMEN GDVYTFGYGQ HGQLGHGDVN SRGCPTLVQA LPGPSTQVTA 

       970        980        990       1000       1010       1020 
GSNHTAVLLM DGQVFTFGSF SKGQLGRPIL DVPYWNAKPA PMPNIGSKYG RKATWIGASG 

      1030       1040       1050       1060       1070       1080 
DQTFLRIDEA LINSHVLATS EIFASKHIIG LVPASISEPP PFKCLLINKV DGSCKTFNDS 

      1090       1100       1110       1120       1130       1140 
EQEDLQGFGV CLDPVYDVIW RFRPNTRELW CYNAVVADAR LPSAADMQSR CSILSPELAL 

      1150       1160       1170       1180       1190       1200 
PTGSRALTTR SHAALHILGC LDTLAAMQDL KMGVASTEEE TQAVMKVYSK EDYSVVNRFE 

      1210       1220       1230       1240       1250       1260 
SHGGGWGYSA HSVEAIRFSA DTDILLGGLG LFGGRGEYTA KIKLFELGPD GGDHETDGDL 

      1270       1280       1290       1300       1310       1320 
LAETDVLAYD CAAREKYAMM FDEPVLLQAG WWYVAWARVS GPSSDCGSHG QASITTDDGV 

      1330       1340       1350       1360       1370       1380 
VFQFKSSKKS NNGTDVNAGQ IPQLLYRLPT SDGSASKGKQ QTSEPVHILK RSFARTVSVE 

      1390       1400       1410       1420       1430       1440 
CFESLLSILH WSWTTLVLGV EELRGLKGFQ FTATLLDLER LRFVGTCCLR LLRVYTCEIY 

      1450       1460       1470       1480       1490       1500 
PVSATGKAVV EETSKLAECI GKTRTLLRKI LSEGVDHCMV KLDNDPQGYL SQPLSLLEAV 

      1510       1520       1530       1540       1550       1560 
LQECHNTFTA CFHSFYPTPA LQWACLCDLL NCLDQDIQEA NFKTSSSRLL AAVMSALCHT 

      1570       1580       1590       1600       1610       1620 
SVKLTSIFPI AYDGEVLLRS IVKQVSTEND STLVHRFPLL VAHMEKLSQS EENISGMTSF 

      1630       1640       1650       1660       1670       1680 
REVLEKMLVI VVLPVRNSLR RENELFSSHL VSNTCGLLAS IVSELTASAL GSEVDGLNSL 

      1690       1700       1710       1720       1730       1740 
HSVKASANRF TKTSQGRSWN TGNGSPDAIC FSVDKPGIVV VGFSVYGGGG IHEYELEVLV 

      1750       1760       1770       1780       1790       1800 
DDSEHAGDST HSHRWTSLEL VKGTYTTDDS PSDIAEIRLD KVVPLKENVK YAVRLRNYGS 

      1810       1820       1830       1840       1850       1860 
RTANGDGGMT TVQCPDGVTF TFSTCSLSSN GTNQTRGQIP QILYYRSEFD GDLQSQLLSK 

      1870       1880       1890       1900       1910       1920 
ANEEDKNCSR ALSVVSTVVR ASKDLLHRAL AVDADDIPEL LSSSSLFSML LPLIIAYIGP 

      1930       1940       1950       1960       1970       1980 
VAAAIPKVAV EVFGLVQQLL PSVAILNQKY APPAFNPNQS TDSTTGNQPE QGLSACTTSS 

      1990       2000       2010       2020       2030       2040 
HYAVIESEHP YKPACVMHYK VTFPECVRWM TIEFDPQCGT AQSEDVLRLL IPVRTVQNSG 

      2050       2060       2070       2080       2090       2100 
YGPKLTSVHE NLNSWIELKK FSGSSGWPTM VLVLPGNEAL FSLETASDYV KDDKASFYGF 

      2110       2120       2130       2140       2150       2160 
KCFAIGYEFS PGPDEGVIQL EKELANLGGV CAAALMKKDL ALPIGNELEE DLEILEEAAL 

      2170       2180       2190       2200       2210       2220 
QVCKTHSGIL GKGLALSHSP TILEALEGNL PLQIQSNEQS FLDDFIACVP GSSGGRLARW 

      2230       2240       2250       2260       2270       2280 
LQPDSYADPQ KTSLILNKDD IRCGWPTTIT VQTKDQYGDV VHVPNMKVEV KAVPVSQKKM 

      2290       2300       2310       2320       2330       2340 
SLQQDQAKKP QRIPGSPAVT AASSNTDMTY GGLASPKLDV SYEPMIVKEA RYIAITMMKV 

      2350       2360       2370       2380       2390       2400 
YENYSFEELR FASPTPKRPS ENMLIRVNND GTYCANWTPG AIGLYTLHVT IDGIEIDAGL 

      2410       2420       2430       2440       2450       2460 
EVKVKDPPKG MIPPGTQLVK PKSEPQPNKV RKFVAKDSAG LRIRSHPSLQ SEQIGIVKVN 

      2470       2480       2490       2500       2510       2520 
GTITFIDEIH NDDGVWLRLN DETIKKYVPN MNGYTEAWCL SFNQHLGKSL LVPVDESKTN 

      2530       2540       2550       2560       2570       2580 
TDDFFKDINS CCPQEATMQE QDMPFLRGGP GMYKVVKTGP SGHNIRSCPN LRGIPIGMLV 

      2590       2600       2610       2620       2630       2640 
LGNKVKAVGE VTNSEGTWVQ LDQNSMVEFC ESDEGEAWSL ARDRGGNQYL RHEDEQALLD 

      2650       2660       2670       2680       2690       2700 
QNSQTPPPSP FSVQAFNKGA SCSAQGFDYG LGNSKGDRGN ISTSSKPAST SGKSELSSKH 

      2710       2720       2730       2740       2750       2760 
SRSLKPDGRM SRTTADQKKP RGTESLSASE SLILKSDAAK LRSDSHSRSL SPNHNTLQTL 

      2770       2780       2790       2800       2810       2820 
KSDGRMPSSS RAESPGPGSR LSSPKPKTLP ANRSSPSGAS SPRSSSPHDK NLPQKSTAPV 

      2830       2840       2850       2860       2870       2880 
KTKLDPPRER SKSDSYTLDP DTLRKKKMPL TEPLRGRSTS PKPKSVPKDS TDSPGSENRA 

      2890       2900       2910       2920       2930       2940 
PSPHVVQENL HSEVVEVCTS STLKTNSLTD STCDDSSEFK SVDEGSNKVH FSIGKAPLKD 

      2950       2960       2970       2980       2990       3000 
EQEMRASPKI SRKCANRHTR PKKEKSSFLF KGDGSKPLEP AKQAMSPSVA ECARAVFASF 

      3010       3020       3030       3040       3050       3060 
LWHEGIVHDA MACSSFLKFH PELSKEHAPI RSSLNSQQPT EEKETKLKNR HSLEISSALN 

      3070       3080       3090       3100       3110       3120 
MFNIAPHGPD ISKMGSINKN KVLSMLKEPP LHEKCEDGKT ETTFEMSMHN TMKSKSPLPL 

      3130       3140       3150       3160       3170       3180 
TLQHLVAFWE DISLATIKAA SQNMIFPSPG SCAVLKKKEC EKENKKSKKE KKKKEKAEVR 

      3190       3200       3210       3220       3230       3240 
PRGNLFGEMA QLAVGGPEKD TICELCGESH PYPVTYHMRQ AHPGCGRYAG GQGYNSIGHF 

      3250       3260       3270       3280       3290       3300 
CGGWAGNCGD GGIGGSTWYL VCDRCREKYL REKQAAAREK VKQSRRKPMQ VKTPRALPTM 

      3310       3320       3330       3340       3350       3360 
EAHQVIKANA LFLLSLSSAA EPSILCYHPA KPFQSQLPSV KEGISEDLPV KMPCLYLQTL 

      3370       3380       3390       3400       3410       3420 
ARHHHENFVG YQDDNLFQDE MRYLRSTSVP APYISVTPDA SPNVFEEPES NMKSMPPSLE 

      3430       3440       3450       3460       3470       3480 
TSPITDTDLA KRTVFQRSYS VVASEYDKQH SILPARVKAI PRRRVNSGDT EVGSSLLRHP 

      3490       3500       3510       3520       3530       3540 
SPELSRLISA HSSLSKGERN FQWPVLAFVI QHHDLEGLEI AMKQALRKSA CRVFAMEAFN 

      3550       3560       3570       3580       3590       3600 
WLLCNVIQTT SLHDILWHFV ASLTPAPVEP EEEEDEENKT SKENSEQEKD TRVCEHPLSD 

      3610       3620       3630       3640       3650       3660 
IVIAGEAAHP LPHTFHRLLQ TISDLMMSLP SGSSLQQMAL RCWSLKFKQS DHQFLHQSNV 

      3670       3680       3690       3700       3710       3720 
FHHINNILSK SDDGDSEESF SISIQSGFEA MSQELCIVMC LKDLTSIVDI KTSSRPAMIG 

      3730       3740       3750       3760       3770       3780 
SLTDGSTETF WESGDEDKNK TKNITINCVK GINARYVSVH VDNSRDLGNK VTSMTFLTGK 

      3790       3800       3810       3820       3830       3840 
AVEDLCRIKQ VDLDSRHIGW VTSELPGGDN HIIKIELKGP ENTLRVRQVK VLGWKDGEST 

      3850       3860       3870       3880       3890       3900 
KIAGQISASV AQQRNCEAET LRVFRLITSQ VFGKLISGDA EPTPEQEEKA LLSSPEGEEK 

      3910       3920       3930       3940       3950       3960 
VYNATSDADL KEHMVGIIFS RSKLTNLQKQ VCAHIVQAIR MEATRVREEW EHAISSKENA 

      3970       3980       3990       4000       4010       4020 
NSQPNDEDAS SDAYCFELLS MVLALSGSNV GRQYLAQQLT LLQDLFSLLH TASPRVQRQV 

      4030       4040       4050       4060       4070       4080 
TSLLRRVLPE VTPSRLASII GVKSLPPADI SDIIHSTEKG DWNKLGILDM FLGCIAKALT 

      4090       4100       4110       4120       4130       4140 
VQLKAKGTTI TGTAGTTVGK GVTTVTLPMI FNSSYLRRGE SHWWMKGSTP TQISEIIIKL 

      4150       4160       4170       4180       4190       4200 
IKDMAAGHLS EAWSRVTKNA IAETIIALTK MEEEFRSPVR CIATTRLWLA LASLCVLDQD 

      4210       4220       4230       4240       4250       4260 
HVDRLSSGRW MGKDGQQKQM PMCDNHDDGE TAAIILCNVC GNLCTDCDRF LHLHRRTKTH 

      4270       4280       4290       4300       4310       4320 
QRQVFKEEEE AIKVDLHEGC GRTKLFWLMA LADSKTMKAM VEFREHTGKP TTSSSEACRF 

      4330       4340       4350       4360       4370       4380 
CGSRSGTELS AVGSVCSDAD CQEYAKIACS KTHPCGHPCG GVKNEEHCLP CLHGCDKSAT 

      4390       4400       4410       4420       4430       4440 
SLKQDADDMC MICFTEALSA APAIQLDCSH IFHLQCCRRV LENRWLGPRI TFGFISCPIC 

      4450       4460       4470       4480       4490       4500 
KNKINHIVLK DLLDPIKELY EDVRRKALMR LEYEGLHKSE AITTPGVRFY NDPAGYAMNR 

      4510       4520       4530       4540       4550       4560 
YAYYVCYKCR KAYFGGEARC DAEAGRGDDY DPRELICGAC SDVSRAQMCP KHGTDFLEYK 

      4570       4580       4590       4600       4610       4620 
CRYCCSVAVF FCFGTTHFCN ACHDDFQRMT SIPKEELPHC PAGPKGKQLE GTECPLHVVH 

      4630       4640 
PPTGEEFALG CGVCRNAHTF 

« Hide

Isoform 2 [UniParc].

Checksum: A54457A00B327ADA
Show »

FASTA4,637509,708

References

« Hide 'large scale' references
[1]"Identification of a large Myc-binding protein that contains RCC1-like repeats."
Guo Q., Xie J., Dang C.V., Liu E.T., Bishop J.M.
Proc. Natl. Acad. Sci. U.S.A. 95:9172-9177(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYC, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3431-4640 (ISOFORM 1).
Tissue: Brain.
[5]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3789-4640 (ISOFORM 1).
Tissue: Brain.
[7]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4367-4640.
Tissue: Umbilical cord blood.
[8]"Pam and its ortholog highwire interact with and may negatively regulate the TSC1.TSC2 complex."
Murthy V., Han S., Beauchamp R.L., Smith N., Haddad L.A., Ito N., Ramesh V.
J. Biol. Chem. 279:1351-1358(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TSC2.
[9]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3052, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Pam (Protein associated with Myc) functions as an E3 ubiquitin ligase and regulates TSC/mTOR signaling."
Han S., Witt R.M., Santos T.M., Polizzano C., Sabatini B.L., Ramesh V.
Cell. Signal. 20:1084-1091(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, AUTOUBIQUITINATION.
[11]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-143; SER-145; THR-2645; SER-2751; SER-3440 AND SER-3467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development."
Saiga T., Fukuda T., Matsumoto M., Tada H., Okano H.J., Okano H., Nakayama K.I.
Mol. Cell. Biol. 29:3529-3543(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH FBXO45.
[15]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2645; SER-2882 AND SER-3467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[17]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2645 AND SER-3467, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[18]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[19]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF075587 mRNA. Translation: AAC39928.1.
AL159154 expand/collapse EMBL AC list , AL159158, AL359257, AC001226 Genomic DNA. Translation: CAH73736.1.
AL159158 expand/collapse EMBL AC list , AL159154, AL359257, AC001226 Genomic DNA. Translation: CAI16842.1.
AL359257 expand/collapse EMBL AC list , AL159158, AL159154, AC001226 Genomic DNA. Translation: CAI39759.1.
AL359257, AC001226 Genomic DNA. Translation: CAI39758.1. Sequence problems.
CH471093 Genomic DNA. Translation: EAW80565.1.
AB020723 mRNA. Translation: BAA74939.1.
BC037971 mRNA. Translation: AAH37971.1. Different initiation.
AF083244 mRNA. Translation: AAD39842.1. Sequence problems.
RefSeqNP_055872.4. NM_015057.4.
UniGeneHs.591221.

3D structure databases

ProteinModelPortalO75592.
SMRO75592. Positions 496-677, 880-1022, 1189-1349, 1688-1847, 2366-2397, 4383-4447.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116709. 24 interactions.
DIPDIP-28142N.
IntActO75592. 35 interactions.
MINTMINT-1632199.
STRING9606.ENSP00000349892.

PTM databases

PhosphoSiteO75592.

Proteomic databases

MaxQBO75592.
PaxDbO75592.
PRIDEO75592.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000357337; ENSP00000349892; ENSG00000005810. [O75592-1]
ENST00000544440; ENSP00000444596; ENSG00000005810. [O75592-1]
GeneID23077.
KEGGhsa:23077.
UCSCuc010aev.3. human. [O75592-1]

Organism-specific databases

CTD23077.
GeneCardsGC13M077618.
HGNCHGNC:23386. MYCBP2.
HPAHPA039945.
MIM610392. gene.
neXtProtNX_O75592.
PharmGKBPA134871126.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240798.
HOVERGENHBG053153.
InParanoidO75592.
KOK10693.
OrthoDBEOG75B84C.
PhylomeDBO75592.
TreeFamTF313151.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressO75592.
BgeeO75592.
CleanExHS_MYCBP2.
HS_PAM.
GenevestigatorO75592.

Family and domain databases

Gene3D2.130.10.30. 2 hits.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
3.30.40.10. 1 hit.
InterProIPR004939. APC_su10/DOC_dom.
IPR017868. Filamin/ABP280_repeat-like.
IPR008979. Galactose-bd-like.
IPR027047. Highwire/Pam/Rpm-1.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR012983. PHR.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
IPR003646. SH3-like_bac-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERPTHR12846:SF14. PTHR12846:SF14. 1 hit.
PfamPF00630. Filamin. 1 hit.
PF08005. PHR. 2 hits.
PF00415. RCC1. 1 hit.
PF08239. SH3_3. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
PRINTSPR00633. RCCNDNSATION.
SMARTSM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF50985. SSF50985. 2 hits.
SSF81296. SSF81296. 2 hits.
PROSITEPS51284. DOC. 1 hit.
PS50194. FILAMIN_REPEAT. 1 hit.
PS00626. RCC1_2. 2 hits.
PS50012. RCC1_3. 3 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMYCBP2. human.
GeneWikiMYCBP2.
GenomeRNAi23077.
NextBio44191.
PROO75592.
SOURCESearch...

Entry information

Entry nameMYCB2_HUMAN
AccessionPrimary (citable) accession number: O75592
Secondary accession number(s): A6NJC6 expand/collapse secondary AC list , Q5JSX8, Q5VZN6, Q6PIB6, Q9UQ11, Q9Y6E4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM