O75575 (RPC9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase III subunit RPC9
Short name=RNA polymerase III subunit C9

Alternative name(s):
Calcitonin gene-related peptide-receptor component protein
Short name=CGRP-RCP
Short name=CGRP-receptor component protein
Short name=CGRPRCP
Short name=HsC17

Gene names

Name:

CRCP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity. Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues.
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. Interacts with POLR3H/RPC8. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex. Ref.9 Ref.10
Subcellular location	Nucleus By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Tissue specificity	Ubiquitous. Most prevalent in testis. Ref.2
Sequence similarities	Belongs to the eukaryotic RPC9 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Antiviral defense Immunity Innate immunity Transcription
Cellular component	Cell membrane DNA-directed RNA polymerase Membrane Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acrosome reaction Non-traceable author statement Ref.2. Source: UniProtKB defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW neuropeptide signaling pathway Inferred from electronic annotation. Source: Compara transcription from RNA polymerase III promoter Inferred from direct assay Ref.10. Source: UniProtKB
Cellular_component	DNA polymerase III complex Inferred from direct assay Ref.10. Source: UniProtKB acrosomal vesicle Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA-directed RNA polymerase activity Inferred from direct assay Ref.10. Source: UniProtKB calcitonin gene-related polypeptide receptor activity Inferred from electronic annotation. Source: Compara calcitonin receptor activity Non-traceable author statement PubMed 8622957. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75575-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75575-2)

The sequence of this isoform differs from the canonical sequence as follows:
     14-46: Missing.

Isoform 3 (identifier: O75575-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MEVKDANSALLSN → MQRGDPGEHLGARSWHLGAVGGGGDSCEVKDANSALLSN
     14-46: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: O75575-4)

The sequence of this isoform differs from the canonical sequence as follows:
     4-80: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	DNA-directed RNA polymerase III subunit RPC9		PRO_0000079335
Natural variations
	Alternative sequence	1 – 13	13	MEVKD…ALLSN → MQRGDPGEHLGARSWHLGAV GGGGDSCEVKDANSALLSN in isoform 3.		VSP_043249
	Alternative sequence	4 – 80	77	Missing in isoform 4.		VSP_046273
	Alternative sequence	14 – 46	33	Missing in isoform 2 and isoform 3.		VSP_007538

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 75943BA57A63AF3D	FASTA	148	16,871
	10 20 30 40 50 60 MEVKDANSAL LSNYEVFQLL TDLKEQRKES GKNKHSSGQQ NLNTITYETL KYISKTPCRH 70 80 90 100 110 120 QSPEIVREFL TALKSHKLTK AEKLQLLNHR PVTAVEIQLM VEESEERLTE EQIEALLHTV 130 140 TSILPAEPEA EQKKNTNSNV AMDEEDPA
	Isoform 2 [UniParc]. Checksum: 60F5C22410882C26	FASTA	115	13,054
	10 20 30 40 50 60 MEVKDANSAL LSNYETLKYI SKTPCRHQSP EIVREFLTAL KSHKLTKAEK LQLLNHRPVT 70 80 90 100 110 AVEIQLMVEE SEERLTEEQI EALLHTVTSI LPAEPEAEQK KNTNSNVAMD EEDPA
	Isoform 3 [UniParc]. Checksum: 0A92F564E3A59C9A	FASTA	141	15,613
	10 20 30 40 50 60 MQRGDPGEHL GARSWHLGAV GGGGDSCEVK DANSALLSNY ETLKYISKTP CRHQSPEIVR 70 80 90 100 110 120 EFLTALKSHK LTKAEKLQLL NHRPVTAVEI QLMVEESEER LTEEQIEALL HTVTSILPAE 130 140 PEAEQKKNTN SNVAMDEEDP A
	Isoform 4 [UniParc]. Checksum: 9CF80A5EFAA7B63A	FASTA	71	8,011
	10 20 30 40 50 60 MEVAEKLQLL NHRPVTAVEI QLMVEESEER LTEEQIEALL HTVTSILPAE PEAEQKKNTN 70 SNVAMDEEDP A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human CGRP-receptor component protein (RCP) ORF." Luebke A.E., Dahl G.P., Dickerson I.M. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Testes exhibit elevated expression of calcitonin gene-related peptide receptor component protein." Balkan W., Oates E.L., Howard G.A., Roos B.A. Endocrinology 140:1459-1469(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Adrenal gland, Brain and Kidney.
[4]	"Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." Kimura K., Wakamatsu A., Suzuki Y., Ota T., Nishikawa T., Yamashita R., Yamamoto J., Sekine M., Tsuritani K., Wakaguri H., Ishii S., Sugiyama T., Saito K., Isono Y., Irie R., Kushida N., Yoneyama T., Otsuka R. , Kanda K., Yokoi T., Kondo H., Wagatsuma M., Murakawa K., Ishida S., Ishibashi T., Takahashi-Fujii A., Tanase T., Nagai K., Kikuchi H., Nakai K., Isogai T., Sugano S. Genome Res. 16:55-65(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Fetal muscle.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Adipose tissue.
[6]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Testis.
[9]	"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits." Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N. Mol. Cell. Biol. 22:8044-8055(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY, INTERACTION WITH RPC8.
[10]	"An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP." Siaut M., Zaros C., Levivier E., Ferri M.L., Court M., Werner M., Callebaut I., Thuriaux P., Sentenac A., Conesa C. Mol. Cell. Biol. 23:195-205(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF073792 mRNA. Translation: AAC25992.1. U51134 mRNA. Translation: AAD05036.1. AK290553 mRNA. Translation: BAF83242.1. AK303737 mRNA. Translation: BAG64710.1. AK311829 mRNA. Translation: BAG34771.1. BP233211 mRNA. No translation available. BX647867 mRNA. Translation: CAI46069.1. AC068533 Genomic DNA. No translation available. CH471140 Genomic DNA. Translation: EAX07941.1. BC040107 mRNA. Translation: AAH40107.1. BC105808 mRNA. Translation: AAI05809.1.
IPI	IPI00004477. IPI00335708. IPI00759701. IPI00927312.
RefSeq	NP_001035737.1. NM_001040647.1. NP_001035738.1. NM_001040648.1. NP_001135886.1. NM_001142414.1. NP_055293.1. NM_014478.4.
UniGene	Hs.300684.
3D structure databases
ProteinModelPortal	O75575.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75575. 2 interactions.
STRING	9606.ENSP00000378736.
PTM databases
PhosphoSite	O75575.
Proteomic databases
PaxDb	O75575.
PeptideAtlas	O75575.
PRIDE	O75575.
Protocols and materials databases
DNASU	27297.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338592; ENSP00000340044; ENSG00000241258. ENST00000395326; ENSP00000378736; ENSG00000241258. ENST00000398684; ENSP00000381674; ENSG00000241258. ENST00000431089; ENSP00000388653; ENSG00000241258.
GeneID	27297.
KEGG	hsa:27297.
UCSC	uc003tus.3. human. uc003tut.3. human.
Organism-specific databases
CTD	27297.
GeneCards	GC07P065578.
HGNC	HGNC:17888. CRCP.
HPA	HPA007216.
MIM	606121. gene.
neXtProt	NX_O75575.
PharmGKB	PA164718123.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG252343.
HOGENOM	HOG000244081.
HOVERGEN	HBG023697.
OMA	KHSAGQQ.
PhylomeDB	O75575.
Gene expression databases
ArrayExpress	O75575.
Bgee	O75575.
Genevestigator	O75575.
GermOnline	ENSG00000126522. Homo sapiens.
Family and domain databases
InterPro	IPR010997. HRDC-like. IPR005574. RNA_pol_II_Rpb4. IPR006590. RNA_pol_II_Rpb4_core. [Graphical view]
Pfam	PF03874. RNA_pol_Rpb4. 1 hit. [Graphical view]
SMART	SM00657. RPOL4c. 1 hit. [Graphical view]
SUPFAM	SSF47819. HRDC_like. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	27297.
NextBio	50270.
SOURCE	Search...

Entry information

Entry name

RPC9_HUMAN

Accession

Primary (citable) accession number: O75575
Secondary accession number(s): A8MUZ4 , A8MW23, B2R4H4, B4E198, Q3KRA3, Q5HYF1, Q8IXL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.