ID   RF1M_HUMAN              Reviewed;         445 AA.
AC   O75570; B4DG01; Q5T6Y5; Q8IUQ6;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 2.
DT   27-MAR-2024, entry version 180.
DE   RecName: Full=Peptide chain release factor 1, mitochondrial {ECO:0000305};
DE            Short=MRF-1;
DE            Short=MtRF-1 {ECO:0000303|PubMed:36302763, ECO:0000303|PubMed:36596788, ECO:0000303|PubMed:37141370};
DE   Flags: Precursor;
GN   Name=MTRF1 {ECO:0000303|PubMed:36302763, ECO:0000303|PubMed:36596788,
GN   ECO:0000303|PubMed:37141370, ECO:0000312|HGNC:HGNC:7469};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=9838146; DOI=10.1016/s0167-4781(98)00223-1;
RA   Zhang Y., Spremulli L.L.;
RT   "Identification and cloning of human mitochondrial translational release
RT   factor 1 and the ribosome recycling factor.";
RL   Biochim. Biophys. Acta 1443:245-250(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Amygdala;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057823; DOI=10.1038/nature02379;
RA   Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA   Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA   Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA   Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA   Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA   Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA   Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA   Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA   Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA   Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA   Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA   Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA   Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA   Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA   Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA   Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA   Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA   Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA   Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA   Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA   Rogers J., Ross M.T.;
RT   "The DNA sequence and analysis of human chromosome 13.";
RL   Nature 428:522-528(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PROBABLE FUNCTION AS A RELEASE FACTOR, AND SUBCELLULAR LOCATION.
RX   PubMed=17803939; DOI=10.1016/j.molcel.2007.06.031;
RA   Soleimanpour-Lichaei H.R., Kuehl I., Gaisne M., Passos J.F., Wydro M.,
RA   Rorbach J., Temperley R., Bonnefoy N., Tate W., Lightowlers R.,
RA   Chrzanowska-Lightowlers Z.;
RT   "mtRF1a is a human mitochondrial translation release factor decoding the
RT   major termination codons UAA and UAG.";
RL   Mol. Cell 27:745-757(2007).
RN   [6]
RP   CAUTION.
RX   PubMed=22569235; DOI=10.1186/1745-6150-7-14;
RA   Huynen M.A., Duarte I., Chrzanowska-Lightowlers Z.M., Nabuurs S.B.;
RT   "Structure based hypothesis of a mitochondrial ribosome rescue mechanism.";
RL   Biol. Direct 7:14-14(2012).
RN   [7]
RP   FUNCTION.
RX   PubMed=36302763; DOI=10.1038/s41467-022-34088-w;
RA   Nadler F., Lavdovskaia E., Krempler A., Cruz-Zaragoza L.D., Dennerlein S.,
RA   Richter-Dennerlein R.;
RT   "Human mtRF1 terminates COX1 translation and its ablation induces
RT   mitochondrial ribosome-associated quality control.";
RL   Nat. Commun. 13:6406-6406(2022).
RN   [8]
RP   METHYLATION AT GLN-313.
RX   PubMed=35260756; DOI=10.1038/s41598-022-08061-y;
RA   Fang Q., Kimura Y., Shimazu T., Suzuki T., Yamada A., Dohmae N.,
RA   Iwasaki S., Shinkai Y.;
RT   "Mammalian HEMK1 methylates glutamine residue of the GGQ motif of
RT   mitochondrial release factors.";
RL   Sci. Rep. 12:4104-4104(2022).
RN   [9]
RP   FUNCTION, AND MUTAGENESIS OF 311-GLY-GLY-312.
RX   PubMed=36596788; DOI=10.1038/s41467-022-35684-6;
RA   Krueger A., Remes C., Shiriaev D.I., Liu Y., Spaahr H., Wibom R.,
RA   Atanassov I., Nguyen M.D., Cooperman B.S., Rorbach J.;
RT   "Human mitochondria require mtRF1 for translation termination at non-
RT   canonical stop codons.";
RL   Nat. Commun. 14:30-30(2023).
RN   [10] {ECO:0007744|PDB:8OIN, ECO:0007744|PDB:8OIP}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.5 ANGSTROMS) IN COMPLEX WITH
RP   MITOCHONDRIAL RIBOSOME, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=37141370; DOI=10.1126/science.adf9890;
RA   Saurer M., Leibundgut M., Nadimpalli H.P., Scaiola A., Schoenhut T.,
RA   Lee R.G., Siira S.J., Rackham O., Dreos R., Lenarcic T., Kummer E.,
RA   Gatfield D., Filipovska A., Ban N.;
RT   "Molecular basis of translation termination at noncanonical stop codons in
RT   human mitochondria.";
RL   Science 380:531-536(2023).
CC   -!- FUNCTION: Mitochondrial peptide chain release factor that directs the
CC       termination of translation in response to the peptide chain non-
CC       canonical stop codons AGG and AGA (PubMed:36302763, PubMed:36596788,
CC       PubMed:37141370). Non-canonical termination codons AGG and AGA are
CC       found at the end of MT-CO1/COX1 and MT-ND6/ND6 open reading frames,
CC       respectively (PubMed:37141370). Recognizes non-canonical stop codons
CC       via a network of interactions between the codon, MTRF1 and the
CC       ribosomal RNA (rRNA): in contrast to other translation release factors,
CC       which identify the codon in the A-site via direct interactions of amino
CC       acid side chains with the bases, MTRF1 repositions the first 2 bases of
CC       the stop codon to use an intricate network of interactions that
CC       includes residues of the release factor, the rRNA of the small
CC       ribosomal subunit, as well as neighboring bases of the mRNA
CC       (PubMed:37141370). {ECO:0000269|PubMed:36302763,
CC       ECO:0000269|PubMed:36596788, ECO:0000269|PubMed:37141370}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:17803939,
CC       ECO:0000269|PubMed:37141370}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O75570-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O75570-2; Sequence=VSP_055858, VSP_055859;
CC   -!- DOMAIN: The GGQ domain interacts with the peptidyltransferase center
CC       (PTC) of the large ribosomal subunit to trigger nascent chain
CC       hydrolysis. {ECO:0000250|UniProtKB:Q9H3J6}.
CC   -!- PTM: Methylation of glutamine in the GGQ triplet by HEMK1 is conserved
CC       from bacteria to mammals. {ECO:0000269|PubMed:35260756}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought to not act as a peptide chain release
CC       factor because of a sequence insertion in the stop codon-recognition
CC       domain that would prevent interactions with the mRNA (PubMed:22569235).
CC       However, it was later shown to specifically direct the termination of
CC       translation in response to non-canonical termination stop codons AGG
CC       and AGA (PubMed:36302763, PubMed:36596788, PubMed:37141370).
CC       {ECO:0000269|PubMed:36302763, ECO:0000269|PubMed:36596788,
CC       ECO:0000269|PubMed:37141370, ECO:0000305|PubMed:22569235}.
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DR   EMBL; AF072934; AAD12759.1; -; mRNA.
DR   EMBL; AK294343; BAG57612.1; -; mRNA.
DR   EMBL; AL354696; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC042196; AAH42196.1; -; mRNA.
DR   CCDS; CCDS9378.1; -. [O75570-1]
DR   RefSeq; NP_004285.2; NM_004294.2. [O75570-1]
DR   RefSeq; XP_005266656.1; XM_005266599.2.
DR   RefSeq; XP_006719960.1; XM_006719897.2.
DR   RefSeq; XP_006719961.1; XM_006719898.2.
DR   RefSeq; XP_006719963.1; XM_006719900.2.
DR   RefSeq; XP_011533620.1; XM_011535318.2.
DR   RefSeq; XP_011533622.1; XM_011535320.2.
DR   RefSeq; XP_011533623.1; XM_011535321.2.
DR   RefSeq; XP_011533625.1; XM_011535323.2.
DR   RefSeq; XP_011533627.1; XM_011535325.2.
DR   RefSeq; XP_016876351.1; XM_017020862.1.
DR   RefSeq; XP_016876352.1; XM_017020863.1.
DR   RefSeq; XP_016876353.1; XM_017020864.1.
DR   RefSeq; XP_016876354.1; XM_017020865.1.
DR   RefSeq; XP_016876355.1; XM_017020866.1. [O75570-1]
DR   RefSeq; XP_016876356.1; XM_017020867.1.
DR   RefSeq; XP_016876357.1; XM_017020868.1.
DR   RefSeq; XP_016876358.1; XM_017020869.1.
DR   RefSeq; XP_016876362.1; XM_017020873.1.
DR   RefSeq; XP_016876363.1; XM_017020874.1.
DR   RefSeq; XP_016876364.1; XM_017020875.1.
DR   PDB; 8OIN; EM; 3.60 A; Aa=1-445.
DR   PDB; 8OIP; EM; 3.60 A; Aa=1-445.
DR   PDB; 8OIQ; EM; 3.50 A; Aa=1-445.
DR   PDB; 8OIR; EM; 3.10 A; Aa=1-445.
DR   PDB; 8OIS; EM; 3.00 A; Aa=1-445.
DR   PDB; 8OIT; EM; 2.90 A; Aa=1-445.
DR   PDBsum; 8OIN; -.
DR   PDBsum; 8OIP; -.
DR   PDBsum; 8OIQ; -.
DR   PDBsum; 8OIR; -.
DR   PDBsum; 8OIS; -.
DR   PDBsum; 8OIT; -.
DR   AlphaFoldDB; O75570; -.
DR   EMDB; EMD-16894; -.
DR   EMDB; EMD-16895; -.
DR   EMDB; EMD-16896; -.
DR   EMDB; EMD-16897; -.
DR   EMDB; EMD-16898; -.
DR   EMDB; EMD-16899; -.
DR   SMR; O75570; -.
DR   BioGRID; 114978; 243.
DR   IntAct; O75570; 3.
DR   STRING; 9606.ENSP00000368793; -.
DR   iPTMnet; O75570; -.
DR   PhosphoSitePlus; O75570; -.
DR   SwissPalm; O75570; -.
DR   BioMuta; MTRF1; -.
DR   EPD; O75570; -.
DR   jPOST; O75570; -.
DR   MassIVE; O75570; -.
DR   MaxQB; O75570; -.
DR   PaxDb; 9606-ENSP00000368793; -.
DR   PeptideAtlas; O75570; -.
DR   ProteomicsDB; 4093; -.
DR   ProteomicsDB; 50091; -. [O75570-1]
DR   Pumba; O75570; -.
DR   Antibodypedia; 23394; 181 antibodies from 22 providers.
DR   DNASU; 9617; -.
DR   Ensembl; ENST00000379477.5; ENSP00000368790.1; ENSG00000120662.16. [O75570-1]
DR   Ensembl; ENST00000379480.9; ENSP00000368793.3; ENSG00000120662.16. [O75570-1]
DR   GeneID; 9617; -.
DR   KEGG; hsa:9617; -.
DR   MANE-Select; ENST00000379480.9; ENSP00000368793.3; NM_004294.4; NP_004285.2.
DR   UCSC; uc001uxx.4; human. [O75570-1]
DR   AGR; HGNC:7469; -.
DR   CTD; 9617; -.
DR   DisGeNET; 9617; -.
DR   GeneCards; MTRF1; -.
DR   HGNC; HGNC:7469; MTRF1.
DR   HPA; ENSG00000120662; Low tissue specificity.
DR   MIM; 604601; gene.
DR   neXtProt; NX_O75570; -.
DR   OpenTargets; ENSG00000120662; -.
DR   PharmGKB; PA31273; -.
DR   VEuPathDB; HostDB:ENSG00000120662; -.
DR   eggNOG; KOG2726; Eukaryota.
DR   GeneTree; ENSGT00940000156877; -.
DR   HOGENOM; CLU_036856_0_3_1; -.
DR   InParanoid; O75570; -.
DR   OMA; ECQQSRS; -.
DR   OrthoDB; 131884at2759; -.
DR   PhylomeDB; O75570; -.
DR   TreeFam; TF313720; -.
DR   PathwayCommons; O75570; -.
DR   SignaLink; O75570; -.
DR   BioGRID-ORCS; 9617; 47 hits in 1159 CRISPR screens.
DR   ChiTaRS; MTRF1; human.
DR   GeneWiki; MTRF1; -.
DR   GenomeRNAi; 9617; -.
DR   Pharos; O75570; Tbio.
DR   PRO; PR:O75570; -.
DR   Proteomes; UP000005640; Chromosome 13.
DR   RNAct; O75570; Protein.
DR   Bgee; ENSG00000120662; Expressed in body of pancreas and 188 other cell types or tissues.
DR   ExpressionAtlas; O75570; baseline and differential.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0003747; F:translation release factor activity; TAS:ProtInc.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IDA:UniProtKB.
DR   GO; GO:0070126; P:mitochondrial translational termination; IDA:UniProtKB.
DR   Gene3D; 3.30.160.20; -; 1.
DR   Gene3D; 3.30.70.1660; -; 2.
DR   Gene3D; 6.10.140.1950; -; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   PANTHER; PTHR43804; LD18447P; 1.
DR   PANTHER; PTHR43804:SF1; PEPTIDE CHAIN RELEASE FACTOR 1, MITOCHONDRIAL; 1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; Release factor; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
DR   Genevisible; O75570; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Methylation; Mitochondrion;
KW   Protein biosynthesis; Reference proteome; Transit peptide.
FT   TRANSIT         1..61
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           62..445
FT                   /note="Peptide chain release factor 1, mitochondrial"
FT                   /id="PRO_0000030333"
FT   REGION          297..361
FT                   /note="GGQ domain"
FT                   /evidence="ECO:0000250|UniProtKB:Q80VP5"
FT   MOTIF           311..313
FT                   /note="GGQ"
FT                   /evidence="ECO:0000269|PubMed:37141370"
FT   MOD_RES         313
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000269|PubMed:35260756"
FT   VAR_SEQ         1
FT                   /note="M -> MGNGEVVLFSDAEM (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_055858"
FT   VAR_SEQ         409..445
FT                   /note="EFLCGGKGLDQLIQRLLQSADEEAIAELLDEHLKSAK -> AQSHSTGGSRD
FT                   PAHSTFLSLDSVRSPGILIMTSSVRNFYVVGRAWIS (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_055859"
FT   VARIANT         2
FT                   /note="N -> S (in dbSNP:rs9532758)"
FT                   /id="VAR_024603"
FT   VARIANT         324
FT                   /note="L -> V (in dbSNP:rs9566725)"
FT                   /id="VAR_034447"
FT   VARIANT         407
FT                   /note="I -> V (in dbSNP:rs9315812)"
FT                   /id="VAR_051789"
FT   MUTAGEN         311..312
FT                   /note="GG->AA: Impaired mitochondrial peptide chain release
FT                   factor activity."
FT                   /evidence="ECO:0000269|PubMed:36596788"
FT   CONFLICT        63
FT                   /note="H -> L (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        71
FT                   /note="K -> N (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        102
FT                   /note="N -> S (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        123
FT                   /note="I -> T (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        134
FT                   /note="E -> G (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        143
FT                   /note="Q -> R (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        149
FT                   /note="Q -> L (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        224
FT                   /note="L -> P (in Ref. 1; AAD12759)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   445 AA;  52306 MW;  3DC637D90F04C6F7 CRC64;
     MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR
     YCHQDTKMLW KHKALQKYME NLSKEYQTLE QCLQHIPVNE ENRRSLNRRH AELAPLAAIY
     QEIQETEQAI EELESMCKSL NKQDEKQLQE LALEERQTID QKINMLYNEL FQSLVPKEKY
     DKNDVILEVT AGRTTGGDIC QQFTREIFDM YQNYSCYKHW QFELLNYTPA DYGGLHHAAA
     RISGDGVYKH LKYEGGIHRV QRIPEVGLSS RMQRIHTGTM SVIVLPQPDE VDVKLDPKDL
     RIDTFRAKGA GGQHVNKTDS AVRLVHIPTG LVVECQQERS QIKNKEIAFR VLRARLYQQI
     IEKDKRQQQS ARKLQVGTRA QSERIRTYNF TQDRVSDHRI AYEVRDIKEF LCGGKGLDQL
     IQRLLQSADE EAIAELLDEH LKSAK
//