O75570 (RF1M_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1, mitochondrial
Short name=MRF-1
Short name=MtRF-1

Gene names

Name:

MTRF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA. Ref.4
Subcellular location	Mitochondrion Ref.4.
Post-translational modification	Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals By similarity.
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of translational termination Traceable author statement Ref.1. Source: ProtInc
Cellular_component	mitochondrion Traceable author statement Ref.1. Source: ProtInc
Molecular_function	translation release factor activity Traceable author statement Ref.1. Source: ProtInc translation release factor activity, codon specific Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 445		Peptide chain release factor 1, mitochondrial		PRO_0000030333
Amino acid modifications
	Modified residue	313	1	N5-methylglutamine By similarity
Natural variations
	Natural variant	2	1	N → S. Corresponds to variant rs9532758 [ dbSNP | Ensembl ].		VAR_024603
	Natural variant	324	1	L → V. Corresponds to variant rs9566725 [ dbSNP | Ensembl ].		VAR_034447
	Natural variant	407	1	I → V. Corresponds to variant rs9315812 [ dbSNP | Ensembl ].		VAR_051789
Experimental info
	Sequence conflict	63	1	H → L in AAD12759. Ref.1
	Sequence conflict	71	1	K → N in AAD12759. Ref.1
	Sequence conflict	102	1	N → S in AAD12759. Ref.1
	Sequence conflict	123	1	I → T in AAD12759. Ref.1
	Sequence conflict	134	1	E → G in AAD12759. Ref.1
	Sequence conflict	143	1	Q → R in AAD12759. Ref.1
	Sequence conflict	149	1	Q → L in AAD12759. Ref.1
	Sequence conflict	224	1	L → P in AAD12759. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O75570 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: 3DC637D90F04C6F7	FASTA	445	52,306
	10 20 30 40 50 60 MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR 70 80 90 100 110 120 YCHQDTKMLW KHKALQKYME NLSKEYQTLE QCLQHIPVNE ENRRSLNRRH AELAPLAAIY 130 140 150 160 170 180 QEIQETEQAI EELESMCKSL NKQDEKQLQE LALEERQTID QKINMLYNEL FQSLVPKEKY 190 200 210 220 230 240 DKNDVILEVT AGRTTGGDIC QQFTREIFDM YQNYSCYKHW QFELLNYTPA DYGGLHHAAA 250 260 270 280 290 300 RISGDGVYKH LKYEGGIHRV QRIPEVGLSS RMQRIHTGTM SVIVLPQPDE VDVKLDPKDL 310 320 330 340 350 360 RIDTFRAKGA GGQHVNKTDS AVRLVHIPTG LVVECQQERS QIKNKEIAFR VLRARLYQQI 370 380 390 400 410 420 IEKDKRQQQS ARKLQVGTRA QSERIRTYNF TQDRVSDHRI AYEVRDIKEF LCGGKGLDQL 430 440 IQRLLQSADE EAIAELLDEH LKSAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor." Zhang Y., Spremulli L.L. Biochim. Biophys. Acta 1443:245-250(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG." Soleimanpour-Lichaei H.R., Kuehl I., Gaisne M., Passos J.F., Wydro M., Rorbach J., Temperley R., Bonnefoy N., Tate W., Lightowlers R., Chrzanowska-Lightowlers Z. Mol. Cell 27:745-757(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROBABLE FUNCTION AS A RELEASE FACTOR, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF072934 mRNA. Translation: AAD12759.1. AL354696 Genomic DNA. Translation: CAI16615.1. AL354696 Genomic DNA. Translation: CAI16617.1. BC042196 mRNA. Translation: AAH42196.1.
IPI	IPI00026157.
RefSeq	NP_004285.2. NM_004294.2.
UniGene	Hs.382176.
3D structure databases
ProteinModelPortal	O75570.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75570. 1 interaction.
STRING	9606.ENSP00000368790.
PTM databases
PhosphoSite	O75570.
Proteomic databases
PaxDb	O75570.
PRIDE	O75570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379477; ENSP00000368790; ENSG00000120662. ENST00000379480; ENSP00000368793; ENSG00000120662. ENST00000452359; ENSP00000399279; ENSG00000120662.
GeneID	9617.
KEGG	hsa:9617.
UCSC	uc001uxx.3. human.
Organism-specific databases
CTD	9617.
GeneCards	GC13M041790.
HGNC	HGNC:7469. MTRF1.
HPA	HPA043316.
MIM	604601. gene.
neXtProt	NX_O75570.
PharmGKB	PA31273.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0216.
HOGENOM	HOG000074815.
HOVERGEN	HBG017071.
InParanoid	O75570.
KO	K02835.
OrthoDB	EOG48KRBQ.
PhylomeDB	O75570.
Gene expression databases
ArrayExpress	O75570.
Bgee	O75570.
CleanEx	HS_MTRF1.
Genevestigator	O75570.
GermOnline	ENSG00000120662. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
InterPro	IPR014720. dsRNA-bd-like_dom. IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9617.
NextBio	36079.
SOURCE	Search...

Entry information

Entry name

RF1M_HUMAN

Accession

Primary (citable) accession number: O75570
Secondary accession number(s): Q5T6Y5, Q8IUQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	April 12, 2005
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.