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O75570

- RF1M_HUMAN

UniProt

O75570 - RF1M_HUMAN

Protein

Peptide chain release factor 1, mitochondrial

Gene

MTRF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.

    GO - Molecular functioni

    1. translation release factor activity Source: ProtInc
    2. translation release factor activity, codon specific Source: InterPro

    GO - Biological processi

    1. regulation of translational termination Source: ProtInc

    Keywords - Biological processi

    Protein biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide chain release factor 1, mitochondrial
    Short name:
    MRF-1
    Short name:
    MtRF-1
    Gene namesi
    Name:MTRF1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:7469. MTRF1.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: InterPro
    2. mitochondrion Source: ProtInc

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31273.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 445Peptide chain release factor 1, mitochondrialPRO_0000030333
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei313 – 3131N5-methylglutamineBy similarity

    Post-translational modificationi

    Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals.By similarity

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiO75570.
    PaxDbiO75570.
    PRIDEiO75570.

    PTM databases

    PhosphoSiteiO75570.

    Expressioni

    Gene expression databases

    ArrayExpressiO75570.
    BgeeiO75570.
    CleanExiHS_MTRF1.
    GenevestigatoriO75570.

    Organism-specific databases

    HPAiHPA043316.

    Interactioni

    Protein-protein interaction databases

    BioGridi114978. 1 interaction.
    IntActiO75570. 1 interaction.
    STRINGi9606.ENSP00000368790.

    Structurei

    3D structure databases

    ProteinModelPortaliO75570.
    SMRiO75570. Positions 104-438.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0216.
    HOGENOMiHOG000074815.
    HOVERGENiHBG017071.
    InParanoidiO75570.
    KOiK02835.
    OrthoDBiEOG7DRJ33.
    PhylomeDBiO75570.
    TreeFamiTF313720.

    Family and domain databases

    Gene3Di3.30.160.20. 1 hit.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR005139. PCRF.
    IPR000352. Pep_chain_release_fac_I_II.
    [Graphical view]
    PfamiPF03462. PCRF. 1 hit.
    PF00472. RF-1. 1 hit.
    [Graphical view]
    SMARTiSM00937. PCRF. 1 hit.
    [Graphical view]
    PROSITEiPS00745. RF_PROK_I. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O75570-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL    50
    HLLSKNWSRR YCHQDTKMLW KHKALQKYME NLSKEYQTLE QCLQHIPVNE 100
    ENRRSLNRRH AELAPLAAIY QEIQETEQAI EELESMCKSL NKQDEKQLQE 150
    LALEERQTID QKINMLYNEL FQSLVPKEKY DKNDVILEVT AGRTTGGDIC 200
    QQFTREIFDM YQNYSCYKHW QFELLNYTPA DYGGLHHAAA RISGDGVYKH 250
    LKYEGGIHRV QRIPEVGLSS RMQRIHTGTM SVIVLPQPDE VDVKLDPKDL 300
    RIDTFRAKGA GGQHVNKTDS AVRLVHIPTG LVVECQQERS QIKNKEIAFR 350
    VLRARLYQQI IEKDKRQQQS ARKLQVGTRA QSERIRTYNF TQDRVSDHRI 400
    AYEVRDIKEF LCGGKGLDQL IQRLLQSADE EAIAELLDEH LKSAK 445
    Length:445
    Mass (Da):52,306
    Last modified:April 12, 2005 - v2
    Checksum:i3DC637D90F04C6F7
    GO
    Isoform 2 (identifier: O75570-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MGNGEVVLFSDAEM
         409-445: EFLCGGKGLD...LLDEHLKSAK → AQSHSTGGSR...FYVVGRAWIS

    Note: No experimental confirmation available.

    Show »
    Length:468
    Mass (Da):54,622
    Checksum:iC344E292BE468528
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti63 – 631H → L in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti71 – 711K → N in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti102 – 1021N → S in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti123 – 1231I → T in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti134 – 1341E → G in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti143 – 1431Q → R in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti149 – 1491Q → L in AAD12759. (PubMed:9838146)Curated
    Sequence conflicti224 – 2241L → P in AAD12759. (PubMed:9838146)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti2 – 21N → S.
    Corresponds to variant rs9532758 [ dbSNP | Ensembl ].
    VAR_024603
    Natural varianti324 – 3241L → V.
    Corresponds to variant rs9566725 [ dbSNP | Ensembl ].
    VAR_034447
    Natural varianti407 – 4071I → V.
    Corresponds to variant rs9315812 [ dbSNP | Ensembl ].
    VAR_051789

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MGNGEVVLFSDAEM in isoform 2. 1 PublicationVSP_055858
    Alternative sequencei409 – 44537EFLCG…LKSAK → AQSHSTGGSRDPAHSTFLSL DSVRSPGILIMTSSVRNFYV VGRAWIS in isoform 2. 1 PublicationVSP_055859Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF072934 mRNA. Translation: AAD12759.1.
    AK294343 mRNA. Translation: BAG57612.1.
    AL354696 Genomic DNA. Translation: CAI16615.1.
    AL354696 Genomic DNA. Translation: CAI16617.1.
    BC042196 mRNA. Translation: AAH42196.1.
    CCDSiCCDS9378.1.
    RefSeqiNP_004285.2. NM_004294.2.
    XP_005266656.1. XM_005266599.1.
    XP_006719960.1. XM_006719897.1.
    XP_006719961.1. XM_006719898.1.
    XP_006719962.1. XM_006719899.1.
    XP_006719963.1. XM_006719900.1.
    UniGeneiHs.382176.

    Genome annotation databases

    EnsembliENST00000379477; ENSP00000368790; ENSG00000120662. [O75570-1]
    ENST00000379480; ENSP00000368793; ENSG00000120662. [O75570-1]
    ENST00000430347; ENSP00000400031; ENSG00000120662. [O75570-2]
    GeneIDi9617.
    KEGGihsa:9617.
    UCSCiuc001uxx.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF072934 mRNA. Translation: AAD12759.1 .
    AK294343 mRNA. Translation: BAG57612.1 .
    AL354696 Genomic DNA. Translation: CAI16615.1 .
    AL354696 Genomic DNA. Translation: CAI16617.1 .
    BC042196 mRNA. Translation: AAH42196.1 .
    CCDSi CCDS9378.1.
    RefSeqi NP_004285.2. NM_004294.2.
    XP_005266656.1. XM_005266599.1.
    XP_006719960.1. XM_006719897.1.
    XP_006719961.1. XM_006719898.1.
    XP_006719962.1. XM_006719899.1.
    XP_006719963.1. XM_006719900.1.
    UniGenei Hs.382176.

    3D structure databases

    ProteinModelPortali O75570.
    SMRi O75570. Positions 104-438.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114978. 1 interaction.
    IntActi O75570. 1 interaction.
    STRINGi 9606.ENSP00000368790.

    PTM databases

    PhosphoSitei O75570.

    Proteomic databases

    MaxQBi O75570.
    PaxDbi O75570.
    PRIDEi O75570.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000379477 ; ENSP00000368790 ; ENSG00000120662 . [O75570-1 ]
    ENST00000379480 ; ENSP00000368793 ; ENSG00000120662 . [O75570-1 ]
    ENST00000430347 ; ENSP00000400031 ; ENSG00000120662 . [O75570-2 ]
    GeneIDi 9617.
    KEGGi hsa:9617.
    UCSCi uc001uxx.3. human.

    Organism-specific databases

    CTDi 9617.
    GeneCardsi GC13M041790.
    HGNCi HGNC:7469. MTRF1.
    HPAi HPA043316.
    MIMi 604601. gene.
    neXtProti NX_O75570.
    PharmGKBi PA31273.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0216.
    HOGENOMi HOG000074815.
    HOVERGENi HBG017071.
    InParanoidi O75570.
    KOi K02835.
    OrthoDBi EOG7DRJ33.
    PhylomeDBi O75570.
    TreeFami TF313720.

    Miscellaneous databases

    GeneWikii MTRF1.
    GenomeRNAii 9617.
    NextBioi 36079.
    PROi O75570.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75570.
    Bgeei O75570.
    CleanExi HS_MTRF1.
    Genevestigatori O75570.

    Family and domain databases

    Gene3Di 3.30.160.20. 1 hit.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR005139. PCRF.
    IPR000352. Pep_chain_release_fac_I_II.
    [Graphical view ]
    Pfami PF03462. PCRF. 1 hit.
    PF00472. RF-1. 1 hit.
    [Graphical view ]
    SMARTi SM00937. PCRF. 1 hit.
    [Graphical view ]
    PROSITEi PS00745. RF_PROK_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor."
      Zhang Y., Spremulli L.L.
      Biochim. Biophys. Acta 1443:245-250(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Amygdala.
    3. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    5. "mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG."
      Soleimanpour-Lichaei H.R., Kuehl I., Gaisne M., Passos J.F., Wydro M., Rorbach J., Temperley R., Bonnefoy N., Tate W., Lightowlers R., Chrzanowska-Lightowlers Z.
      Mol. Cell 27:745-757(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROBABLE FUNCTION AS A RELEASE FACTOR, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiRF1M_HUMAN
    AccessioniPrimary (citable) accession number: O75570
    Secondary accession number(s): B4DG01, Q5T6Y5, Q8IUQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3