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O75570 (RF1M_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 1, mitochondrial

Short name=MRF-1
Short name=MtRF-1
Gene names
Name:MTRF1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA. Ref.4

Subcellular location

Mitochondrion Ref.4.

Post-translational modification

Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals By similarity.

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   Coding sequence diversityPolymorphism
   DomainTransit peptide
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of translational termination

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentmitochondrion

Traceable author statement Ref.1. Source: ProtInc

   Molecular_functiontranslation release factor activity

Traceable author statement Ref.1. Source: ProtInc

translation release factor activity, codon specific

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 445Peptide chain release factor 1, mitochondrialPRO_0000030333

Amino acid modifications

Modified residue3131N5-methylglutamine By similarity

Natural variations

Natural variant21N → S.
Corresponds to variant rs9532758 [ dbSNP | Ensembl ].
VAR_024603
Natural variant3241L → V.
Corresponds to variant rs9566725 [ dbSNP | Ensembl ].
VAR_034447
Natural variant4071I → V.
Corresponds to variant rs9315812 [ dbSNP | Ensembl ].
VAR_051789

Experimental info

Sequence conflict631H → L in AAD12759. Ref.1
Sequence conflict711K → N in AAD12759. Ref.1
Sequence conflict1021N → S in AAD12759. Ref.1
Sequence conflict1231I → T in AAD12759. Ref.1
Sequence conflict1341E → G in AAD12759. Ref.1
Sequence conflict1431Q → R in AAD12759. Ref.1
Sequence conflict1491Q → L in AAD12759. Ref.1
Sequence conflict2241L → P in AAD12759. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O75570 [UniParc].

Last modified April 12, 2005. Version 2.
Checksum: 3DC637D90F04C6F7

FASTA44552,306
        10         20         30         40         50         60 
MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR 

        70         80         90        100        110        120 
YCHQDTKMLW KHKALQKYME NLSKEYQTLE QCLQHIPVNE ENRRSLNRRH AELAPLAAIY 

       130        140        150        160        170        180 
QEIQETEQAI EELESMCKSL NKQDEKQLQE LALEERQTID QKINMLYNEL FQSLVPKEKY 

       190        200        210        220        230        240 
DKNDVILEVT AGRTTGGDIC QQFTREIFDM YQNYSCYKHW QFELLNYTPA DYGGLHHAAA 

       250        260        270        280        290        300 
RISGDGVYKH LKYEGGIHRV QRIPEVGLSS RMQRIHTGTM SVIVLPQPDE VDVKLDPKDL 

       310        320        330        340        350        360 
RIDTFRAKGA GGQHVNKTDS AVRLVHIPTG LVVECQQERS QIKNKEIAFR VLRARLYQQI 

       370        380        390        400        410        420 
IEKDKRQQQS ARKLQVGTRA QSERIRTYNF TQDRVSDHRI AYEVRDIKEF LCGGKGLDQL 

       430        440 
IQRLLQSADE EAIAELLDEH LKSAK 

« Hide

References

« Hide 'large scale' references
[1]"Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor."
Zhang Y., Spremulli L.L.
Biochim. Biophys. Acta 1443:245-250(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG."
Soleimanpour-Lichaei H.R., Kuehl I., Gaisne M., Passos J.F., Wydro M., Rorbach J., Temperley R., Bonnefoy N., Tate W., Lightowlers R., Chrzanowska-Lightowlers Z.
Mol. Cell 27:745-757(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PROBABLE FUNCTION AS A RELEASE FACTOR, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF072934 mRNA. Translation: AAD12759.1.
AL354696 Genomic DNA. Translation: CAI16615.1.
AL354696 Genomic DNA. Translation: CAI16617.1.
BC042196 mRNA. Translation: AAH42196.1.
CCDSCCDS9378.1.
RefSeqNP_004285.2. NM_004294.2.
XP_005266656.1. XM_005266599.1.
XP_006719960.1. XM_006719897.1.
XP_006719961.1. XM_006719898.1.
XP_006719962.1. XM_006719899.1.
XP_006719963.1. XM_006719900.1.
UniGeneHs.382176.

3D structure databases

ProteinModelPortalO75570.
SMRO75570. Positions 104-438.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114978. 1 interaction.
IntActO75570. 1 interaction.
STRING9606.ENSP00000368790.

PTM databases

PhosphoSiteO75570.

Proteomic databases

MaxQBO75570.
PaxDbO75570.
PRIDEO75570.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000379477; ENSP00000368790; ENSG00000120662.
ENST00000379480; ENSP00000368793; ENSG00000120662.
GeneID9617.
KEGGhsa:9617.
UCSCuc001uxx.3. human.

Organism-specific databases

CTD9617.
GeneCardsGC13M041790.
HGNCHGNC:7469. MTRF1.
HPAHPA043316.
MIM604601. gene.
neXtProtNX_O75570.
PharmGKBPA31273.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0216.
HOGENOMHOG000074815.
HOVERGENHBG017071.
InParanoidO75570.
KOK02835.
OrthoDBEOG7DRJ33.
PhylomeDBO75570.
TreeFamTF313720.

Gene expression databases

ArrayExpressO75570.
BgeeO75570.
CleanExHS_MTRF1.
GenevestigatorO75570.

Family and domain databases

Gene3D3.30.160.20. 1 hit.
InterProIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTSM00937. PCRF. 1 hit.
[Graphical view]
PROSITEPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMTRF1.
GenomeRNAi9617.
NextBio36079.
PROO75570.
SOURCESearch...

Entry information

Entry nameRF1M_HUMAN
AccessionPrimary (citable) accession number: O75570
Secondary accession number(s): Q5T6Y5, Q8IUQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 12, 2005
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM