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O75570

- RF1M_HUMAN

UniProt

O75570 - RF1M_HUMAN

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Protein

Peptide chain release factor 1, mitochondrial

Gene

MTRF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.

GO - Molecular functioni

  1. translation release factor activity Source: ProtInc
  2. translation release factor activity, codon specific Source: InterPro

GO - Biological processi

  1. regulation of translational termination Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1, mitochondrial
Short name:
MRF-1
Short name:
MtRF-1
Gene namesi
Name:MTRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 13

Organism-specific databases

HGNCiHGNC:7469. MTRF1.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31273.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 445Peptide chain release factor 1, mitochondrialPRO_0000030333
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei313 – 3131N5-methylglutamineBy similarity

Post-translational modificationi

Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals.By similarity

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiO75570.
PaxDbiO75570.
PRIDEiO75570.

PTM databases

PhosphoSiteiO75570.

Expressioni

Gene expression databases

BgeeiO75570.
CleanExiHS_MTRF1.
ExpressionAtlasiO75570. baseline and differential.
GenevestigatoriO75570.

Organism-specific databases

HPAiHPA043316.

Interactioni

Protein-protein interaction databases

BioGridi114978. 1 interaction.
IntActiO75570. 1 interaction.
STRINGi9606.ENSP00000368790.

Structurei

3D structure databases

ProteinModelPortaliO75570.
SMRiO75570. Positions 104-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0216.
GeneTreeiENSGT00390000011879.
HOGENOMiHOG000074815.
HOVERGENiHBG017071.
InParanoidiO75570.
KOiK02835.
OrthoDBiEOG7DRJ33.
PhylomeDBiO75570.
TreeFamiTF313720.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O75570-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL
60 70 80 90 100
HLLSKNWSRR YCHQDTKMLW KHKALQKYME NLSKEYQTLE QCLQHIPVNE
110 120 130 140 150
ENRRSLNRRH AELAPLAAIY QEIQETEQAI EELESMCKSL NKQDEKQLQE
160 170 180 190 200
LALEERQTID QKINMLYNEL FQSLVPKEKY DKNDVILEVT AGRTTGGDIC
210 220 230 240 250
QQFTREIFDM YQNYSCYKHW QFELLNYTPA DYGGLHHAAA RISGDGVYKH
260 270 280 290 300
LKYEGGIHRV QRIPEVGLSS RMQRIHTGTM SVIVLPQPDE VDVKLDPKDL
310 320 330 340 350
RIDTFRAKGA GGQHVNKTDS AVRLVHIPTG LVVECQQERS QIKNKEIAFR
360 370 380 390 400
VLRARLYQQI IEKDKRQQQS ARKLQVGTRA QSERIRTYNF TQDRVSDHRI
410 420 430 440
AYEVRDIKEF LCGGKGLDQL IQRLLQSADE EAIAELLDEH LKSAK
Length:445
Mass (Da):52,306
Last modified:April 12, 2005 - v2
Checksum:i3DC637D90F04C6F7
GO
Isoform 2 (identifier: O75570-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGNGEVVLFSDAEM
     409-445: EFLCGGKGLD...LLDEHLKSAK → AQSHSTGGSR...FYVVGRAWIS

Note: No experimental confirmation available.

Show »
Length:468
Mass (Da):54,622
Checksum:iC344E292BE468528
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631H → L in AAD12759. (PubMed:9838146)Curated
Sequence conflicti71 – 711K → N in AAD12759. (PubMed:9838146)Curated
Sequence conflicti102 – 1021N → S in AAD12759. (PubMed:9838146)Curated
Sequence conflicti123 – 1231I → T in AAD12759. (PubMed:9838146)Curated
Sequence conflicti134 – 1341E → G in AAD12759. (PubMed:9838146)Curated
Sequence conflicti143 – 1431Q → R in AAD12759. (PubMed:9838146)Curated
Sequence conflicti149 – 1491Q → L in AAD12759. (PubMed:9838146)Curated
Sequence conflicti224 – 2241L → P in AAD12759. (PubMed:9838146)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21N → S.
Corresponds to variant rs9532758 [ dbSNP | Ensembl ].
VAR_024603
Natural varianti324 – 3241L → V.
Corresponds to variant rs9566725 [ dbSNP | Ensembl ].
VAR_034447
Natural varianti407 – 4071I → V.
Corresponds to variant rs9315812 [ dbSNP | Ensembl ].
VAR_051789

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MGNGEVVLFSDAEM in isoform 2. 1 PublicationVSP_055858
Alternative sequencei409 – 44537EFLCG…LKSAK → AQSHSTGGSRDPAHSTFLSL DSVRSPGILIMTSSVRNFYV VGRAWIS in isoform 2. 1 PublicationVSP_055859Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF072934 mRNA. Translation: AAD12759.1.
AK294343 mRNA. Translation: BAG57612.1.
AL354696 Genomic DNA. Translation: CAI16615.1.
AL354696 Genomic DNA. Translation: CAI16617.1.
BC042196 mRNA. Translation: AAH42196.1.
CCDSiCCDS9378.1. [O75570-1]
RefSeqiNP_004285.2. NM_004294.2. [O75570-1]
XP_005266656.1. XM_005266599.1. [O75570-1]
XP_006719960.1. XM_006719897.1. [O75570-1]
XP_006719961.1. XM_006719898.1. [O75570-1]
XP_006719962.1. XM_006719899.1. [O75570-1]
XP_006719963.1. XM_006719900.1. [O75570-1]
UniGeneiHs.382176.

Genome annotation databases

EnsembliENST00000379477; ENSP00000368790; ENSG00000120662. [O75570-1]
ENST00000379480; ENSP00000368793; ENSG00000120662. [O75570-1]
GeneIDi9617.
KEGGihsa:9617.
UCSCiuc001uxx.3. human. [O75570-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF072934 mRNA. Translation: AAD12759.1 .
AK294343 mRNA. Translation: BAG57612.1 .
AL354696 Genomic DNA. Translation: CAI16615.1 .
AL354696 Genomic DNA. Translation: CAI16617.1 .
BC042196 mRNA. Translation: AAH42196.1 .
CCDSi CCDS9378.1. [O75570-1 ]
RefSeqi NP_004285.2. NM_004294.2. [O75570-1 ]
XP_005266656.1. XM_005266599.1. [O75570-1 ]
XP_006719960.1. XM_006719897.1. [O75570-1 ]
XP_006719961.1. XM_006719898.1. [O75570-1 ]
XP_006719962.1. XM_006719899.1. [O75570-1 ]
XP_006719963.1. XM_006719900.1. [O75570-1 ]
UniGenei Hs.382176.

3D structure databases

ProteinModelPortali O75570.
SMRi O75570. Positions 104-438.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114978. 1 interaction.
IntActi O75570. 1 interaction.
STRINGi 9606.ENSP00000368790.

PTM databases

PhosphoSitei O75570.

Proteomic databases

MaxQBi O75570.
PaxDbi O75570.
PRIDEi O75570.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000379477 ; ENSP00000368790 ; ENSG00000120662 . [O75570-1 ]
ENST00000379480 ; ENSP00000368793 ; ENSG00000120662 . [O75570-1 ]
GeneIDi 9617.
KEGGi hsa:9617.
UCSCi uc001uxx.3. human. [O75570-1 ]

Organism-specific databases

CTDi 9617.
GeneCardsi GC13M041790.
HGNCi HGNC:7469. MTRF1.
HPAi HPA043316.
MIMi 604601. gene.
neXtProti NX_O75570.
PharmGKBi PA31273.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0216.
GeneTreei ENSGT00390000011879.
HOGENOMi HOG000074815.
HOVERGENi HBG017071.
InParanoidi O75570.
KOi K02835.
OrthoDBi EOG7DRJ33.
PhylomeDBi O75570.
TreeFami TF313720.

Miscellaneous databases

GeneWikii MTRF1.
GenomeRNAii 9617.
NextBioi 35471628.
PROi O75570.
SOURCEi Search...

Gene expression databases

Bgeei O75570.
CleanExi HS_MTRF1.
ExpressionAtlasi O75570. baseline and differential.
Genevestigatori O75570.

Family and domain databases

Gene3Di 3.30.160.20. 1 hit.
InterProi IPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
[Graphical view ]
Pfami PF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view ]
SMARTi SM00937. PCRF. 1 hit.
[Graphical view ]
PROSITEi PS00745. RF_PROK_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor."
    Zhang Y., Spremulli L.L.
    Biochim. Biophys. Acta 1443:245-250(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Amygdala.
  3. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "mtRF1a is a human mitochondrial translation release factor decoding the major termination codons UAA and UAG."
    Soleimanpour-Lichaei H.R., Kuehl I., Gaisne M., Passos J.F., Wydro M., Rorbach J., Temperley R., Bonnefoy N., Tate W., Lightowlers R., Chrzanowska-Lightowlers Z.
    Mol. Cell 27:745-757(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE FUNCTION AS A RELEASE FACTOR, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRF1M_HUMAN
AccessioniPrimary (citable) accession number: O75570
Secondary accession number(s): B4DG01, Q5T6Y5, Q8IUQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 12, 2005
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3