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Protein

Src kinase-associated phosphoprotein 2

Gene

SKAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.2 Publications

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: ProtInc

GO - Biological processi

  • B cell activation Source: UniProtKB-KW
  • immune response-activating signal transduction Source: GO_Central
  • negative regulation of cell proliferation Source: Ensembl
  • protein complex assembly Source: ProtInc
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

B-cell activation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000005020-MONOMER.
ReactomeiR-HSA-391160. Signal regulatory protein (SIRP) family interactions.
SignaLinkiO75563.

Names & Taxonomyi

Protein namesi
Recommended name:
Src kinase-associated phosphoprotein 2
Alternative name(s):
Pyk2/RAFTK-associated protein
Retinoic acid-induced protein 70
SKAP55 homolog
Short name:
SKAP-55HOM
Short name:
SKAP-HOM
Src family-associated phosphoprotein 2
Src kinase-associated phosphoprotein 55-related protein
Src-associated adapter protein with PH and SH3 domains
Gene namesi
Name:SKAP2
Synonyms:PRAP, RA70, SAPS, SCAP2, SKAP55R
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:15687. SKAP2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • nucleoplasm Source: HPA
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi8935.
OpenTargetsiENSG00000005020.
PharmGKBiPA162403393.

Polymorphism and mutation databases

BioMutaiSKAP2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002701791 – 359Src kinase-associated phosphoprotein 2Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei9PhosphoserineBy similarity1
Modified residuei75Phosphotyrosine1 Publication1
Modified residuei87PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei151PhosphotyrosineBy similarity1
Modified residuei197PhosphotyrosineCombined sources1
Modified residuei223PhosphoserineBy similarity1
Modified residuei261PhosphotyrosineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei286PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in resting platelets. Phosphorylated by FYN on Tyr-261 upon T-cell activation (Probable). Dephosphorylated on Tyr-75 by PTPN22.Curated5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75563.
MaxQBiO75563.
PaxDbiO75563.
PeptideAtlasiO75563.
PRIDEiO75563.

2D gel databases

OGPiO75563.

PTM databases

iPTMnetiO75563.
PhosphoSitePlusiO75563.

Expressioni

Tissue specificityi

Ubiquitously expressed. Present in platelets (at protein level).3 Publications

Inductioni

By retinoic acid.2 Publications

Gene expression databases

BgeeiENSG00000005020.
CleanExiHS_SKAP2.
ExpressionAtlasiO75563. baseline and differential.
GenevisibleiO75563. HS.

Organism-specific databases

HPAiHPA005560.
HPA024045.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with PTPNS1. Part of a complex consisting of SKAP2, FYB and PTPNS1. Part of a complex consisting of SKAP2, FYB and LILRB3. May interact with actin (By similarity). Interacts with FYB, which is required for SKAP2 protein stability. Interacts with LAT, GRB2, PTK2B and PRAM1. May interact with FYN, HCK and LYN. Interacts with FASLG.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPN22Q9Y2R23EBI-2483161,EBI-1211241

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi114448. 15 interactors.
IntActiO75563. 4 interactors.
STRINGi9606.ENSP00000005587.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OMHX-ray2.90E/F/G/H71-79[»]
ProteinModelPortaliO75563.
SMRiO75563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 219PHPROSITE-ProRule annotationAdd BLAST104
Domaini297 – 358SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 64HomodimerizationBy similarityAdd BLAST51

Domaini

The SH3 domain interacts with FYB and PTK2B.

Sequence similaritiesi

Belongs to the SKAP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IH8Q. Eukaryota.
ENOG410ZVZV. LUCA.
GeneTreeiENSGT00390000017856.
HOGENOMiHOG000231109.
HOVERGENiHBG052827.
InParanoidiO75563.
OMAiCFEICAP.
OrthoDBiEOG091G0HBY.
PhylomeDBiO75563.
TreeFamiTF331055.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNPSSTSSP YPLPEEIRNL LADVETFVAD ILKGENLSKK AKEKRESLIK
60 70 80 90 100
KIKDVKSIYL QEFQDKGDAE DGEEYDDPFA GPPDTISLAS ERYDKDDEAP
110 120 130 140 150
SDGAQFPPIA AQDLPFVLKA GYLEKRRKDH SFLGFEWQKR WCALSKTVFY
160 170 180 190 200
YYGSDKDKQQ KGEFAIDGYS VRMNNTLRKD GKKDCCFEIS APDKRIYQFT
210 220 230 240 250
AASPKDAEEW VQQLKFVLQD MESDIIPEDY DERGELYDDV DHPLPISNPL
260 270 280 290 300
TSSQPIDDEI YEELPEEEED SAPVKVEEQR KMSQDSVHHT SGDKSTDYAN
310 320 330 340 350
FYQGLWDCTG AFSDELSFKR GDVIYILSKE YNRYGWWVGE MKGAIGLVPK

AYIMEMYDI
Length:359
Mass (Da):41,217
Last modified:November 1, 1998 - v1
Checksum:i65F266535A89281E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6S → C in BAA36194 (PubMed:9837776).Curated1
Sequence conflicti352Y → H in BAD96605 (Ref. 7) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029812202A → S.3 PublicationsCorresponds to variant rs1129771dbSNPEnsembl.1
Natural variantiVAR_029813253S → T.Corresponds to variant rs17154402dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014486 mRNA. Translation: BAA36194.1.
AJ004886 mRNA. Translation: CAA06193.1.
AF072166 mRNA. Translation: AAC39924.1.
AF051323 mRNA. Translation: AAC99296.1.
AK313209 mRNA. Translation: BAG36025.1.
AK222885 mRNA. Translation: BAD96605.1.
AC003999 Genomic DNA. Translation: AAS02033.1.
AC011299 Genomic DNA. Translation: AAS07536.1.
CH236948 Genomic DNA. Translation: EAL24231.1.
CH471073 Genomic DNA. Translation: EAW93859.1.
BC002893 mRNA. Translation: AAH02893.1.
BC036044 mRNA. Translation: AAH36044.1.
CCDSiCCDS5400.1.
RefSeqiNP_001290397.1. NM_001303468.1.
NP_003921.2. NM_003930.4.
XP_016868260.1. XM_017012771.1.
UniGeneiHs.200770.
Hs.610137.
Hs.675700.

Genome annotation databases

EnsembliENST00000345317; ENSP00000005587; ENSG00000005020.
GeneIDi8935.
KEGGihsa:8935.
UCSCiuc003syc.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014486 mRNA. Translation: BAA36194.1.
AJ004886 mRNA. Translation: CAA06193.1.
AF072166 mRNA. Translation: AAC39924.1.
AF051323 mRNA. Translation: AAC99296.1.
AK313209 mRNA. Translation: BAG36025.1.
AK222885 mRNA. Translation: BAD96605.1.
AC003999 Genomic DNA. Translation: AAS02033.1.
AC011299 Genomic DNA. Translation: AAS07536.1.
CH236948 Genomic DNA. Translation: EAL24231.1.
CH471073 Genomic DNA. Translation: EAW93859.1.
BC002893 mRNA. Translation: AAH02893.1.
BC036044 mRNA. Translation: AAH36044.1.
CCDSiCCDS5400.1.
RefSeqiNP_001290397.1. NM_001303468.1.
NP_003921.2. NM_003930.4.
XP_016868260.1. XM_017012771.1.
UniGeneiHs.200770.
Hs.610137.
Hs.675700.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OMHX-ray2.90E/F/G/H71-79[»]
ProteinModelPortaliO75563.
SMRiO75563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114448. 15 interactors.
IntActiO75563. 4 interactors.
STRINGi9606.ENSP00000005587.

PTM databases

iPTMnetiO75563.
PhosphoSitePlusiO75563.

Polymorphism and mutation databases

BioMutaiSKAP2.

2D gel databases

OGPiO75563.

Proteomic databases

EPDiO75563.
MaxQBiO75563.
PaxDbiO75563.
PeptideAtlasiO75563.
PRIDEiO75563.

Protocols and materials databases

DNASUi8935.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000345317; ENSP00000005587; ENSG00000005020.
GeneIDi8935.
KEGGihsa:8935.
UCSCiuc003syc.4. human.

Organism-specific databases

CTDi8935.
DisGeNETi8935.
GeneCardsiSKAP2.
H-InvDBHIX0025350.
HGNCiHGNC:15687. SKAP2.
HPAiHPA005560.
HPA024045.
MIMi605215. gene.
neXtProtiNX_O75563.
OpenTargetsiENSG00000005020.
PharmGKBiPA162403393.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IH8Q. Eukaryota.
ENOG410ZVZV. LUCA.
GeneTreeiENSGT00390000017856.
HOGENOMiHOG000231109.
HOVERGENiHBG052827.
InParanoidiO75563.
OMAiCFEICAP.
OrthoDBiEOG091G0HBY.
PhylomeDBiO75563.
TreeFamiTF331055.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000005020-MONOMER.
ReactomeiR-HSA-391160. Signal regulatory protein (SIRP) family interactions.
SignaLinkiO75563.

Miscellaneous databases

ChiTaRSiSKAP2. human.
GeneWikiiSKAP2.
GenomeRNAii8935.
PROiO75563.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000005020.
CleanExiHS_SKAP2.
ExpressionAtlasiO75563. baseline and differential.
GenevisibleiO75563. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKAP2_HUMAN
AccessioniPrimary (citable) accession number: O75563
Secondary accession number(s): A4D173
, Q53GP6, Q75MK6, Q75MZ4, Q9UBZ3, Q9UED8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.