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Protein

Src kinase-associated phosphoprotein 2

Gene

SKAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.2 Publications

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: ProtInc

GO - Biological processi

  • B cell activation Source: UniProtKB-KW
  • immune response-activating signal transduction Source: GO_Central
  • negative regulation of cell proliferation Source: Ensembl
  • protein-containing complex assembly Source: ProtInc
  • signal transduction Source: ProtInc

Keywordsi

Biological processB-cell activation

Enzyme and pathway databases

ReactomeiR-HSA-391160 Signal regulatory protein family interactions
SignaLinkiO75563

Names & Taxonomyi

Protein namesi
Recommended name:
Src kinase-associated phosphoprotein 2
Alternative name(s):
Pyk2/RAFTK-associated protein
Retinoic acid-induced protein 70
SKAP55 homolog
Short name:
SKAP-55HOM
Short name:
SKAP-HOM
Src family-associated phosphoprotein 2
Src kinase-associated phosphoprotein 55-related protein
Src-associated adapter protein with PH and SH3 domains
Gene namesi
Name:SKAP2
Synonyms:PRAP, RA70, SAPS, SCAP2, SKAP55R
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000005020.12
HGNCiHGNC:15687 SKAP2
MIMi605215 gene
neXtProtiNX_O75563

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi8935
OpenTargetsiENSG00000005020
PharmGKBiPA162403393

Polymorphism and mutation databases

BioMutaiSKAP2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002701791 – 359Src kinase-associated phosphoprotein 2Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei9PhosphoserineBy similarity1
Modified residuei75Phosphotyrosine1 Publication1
Modified residuei87PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei151PhosphotyrosineBy similarity1
Modified residuei197PhosphotyrosineCombined sources1
Modified residuei223PhosphoserineBy similarity1
Modified residuei261PhosphotyrosineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei286PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in resting platelets. Phosphorylated by FYN on Tyr-261 upon T-cell activation (Probable). Dephosphorylated on Tyr-75 by PTPN22.Curated5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75563
MaxQBiO75563
PaxDbiO75563
PeptideAtlasiO75563
PRIDEiO75563
ProteomicsDBi50086

2D gel databases

OGPiO75563

PTM databases

iPTMnetiO75563
PhosphoSitePlusiO75563

Expressioni

Tissue specificityi

Ubiquitously expressed. Present in platelets (at protein level).3 Publications

Inductioni

By retinoic acid.2 Publications

Gene expression databases

BgeeiENSG00000005020
CleanExiHS_SKAP2
ExpressionAtlasiO75563 baseline and differential
GenevisibleiO75563 HS

Organism-specific databases

HPAiHPA005560
HPA024045

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with PTPNS1. Part of a complex consisting of SKAP2, FYB1 and PTPNS1. Part of a complex consisting of SKAP2, FYB1 and LILRB3. May interact with actin (By similarity). Interacts with FYB1, which is required for SKAP2 protein stability. Interacts with LAT, GRB2, PTK2B and PRAM1. May interact with FYN, HCK and LYN. Interacts with FASLG.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPN22Q9Y2R23EBI-2483161,EBI-1211241

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi114448, 21 interactors
IntActiO75563, 4 interactors
STRINGi9606.ENSP00000005587

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OMHX-ray2.90E/F/G/H71-79[»]
ProteinModelPortaliO75563
SMRiO75563
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 219PHPROSITE-ProRule annotationAdd BLAST104
Domaini297 – 358SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 64HomodimerizationBy similarityAdd BLAST51

Domaini

The SH3 domain interacts with FYB1 and PTK2B.

Sequence similaritiesi

Belongs to the SKAP family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IH8Q Eukaryota
ENOG410ZVZV LUCA
GeneTreeiENSGT00390000017856
HOGENOMiHOG000231109
HOVERGENiHBG052827
InParanoidiO75563
OMAiWDCTGAL
OrthoDBiEOG091G0HBY
PhylomeDBiO75563
TreeFamiTF331055

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR037781 SKAP_fam
PANTHERiPTHR15129 PTHR15129, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

O75563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNPSSTSSP YPLPEEIRNL LADVETFVAD ILKGENLSKK AKEKRESLIK
60 70 80 90 100
KIKDVKSIYL QEFQDKGDAE DGEEYDDPFA GPPDTISLAS ERYDKDDEAP
110 120 130 140 150
SDGAQFPPIA AQDLPFVLKA GYLEKRRKDH SFLGFEWQKR WCALSKTVFY
160 170 180 190 200
YYGSDKDKQQ KGEFAIDGYS VRMNNTLRKD GKKDCCFEIS APDKRIYQFT
210 220 230 240 250
AASPKDAEEW VQQLKFVLQD MESDIIPEDY DERGELYDDV DHPLPISNPL
260 270 280 290 300
TSSQPIDDEI YEELPEEEED SAPVKVEEQR KMSQDSVHHT SGDKSTDYAN
310 320 330 340 350
FYQGLWDCTG AFSDELSFKR GDVIYILSKE YNRYGWWVGE MKGAIGLVPK

AYIMEMYDI
Length:359
Mass (Da):41,217
Last modified:November 1, 1998 - v1
Checksum:i65F266535A89281E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6S → C in BAA36194 (PubMed:9837776).Curated1
Sequence conflicti352Y → H in BAD96605 (Ref. 7) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_029812202A → S3 PublicationsCorresponds to variant dbSNP:rs1129771Ensembl.1
Natural variantiVAR_029813253S → T. Corresponds to variant dbSNP:rs17154402Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014486 mRNA Translation: BAA36194.1
AJ004886 mRNA Translation: CAA06193.1
AF072166 mRNA Translation: AAC39924.1
AF051323 mRNA Translation: AAC99296.1
AK313209 mRNA Translation: BAG36025.1
AK222885 mRNA Translation: BAD96605.1
AC003999 Genomic DNA Translation: AAS02033.1
AC011299 Genomic DNA Translation: AAS07536.1
CH236948 Genomic DNA Translation: EAL24231.1
CH471073 Genomic DNA Translation: EAW93859.1
BC002893 mRNA Translation: AAH02893.1
BC036044 mRNA Translation: AAH36044.1
CCDSiCCDS5400.1
RefSeqiNP_001290397.1, NM_001303468.1
NP_003921.2, NM_003930.4
XP_016868260.1, XM_017012771.1
UniGeneiHs.200770
Hs.610137
Hs.675700

Genome annotation databases

EnsembliENST00000345317; ENSP00000005587; ENSG00000005020
GeneIDi8935
KEGGihsa:8935
UCSCiuc003syc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSKAP2_HUMAN
AccessioniPrimary (citable) accession number: O75563
Secondary accession number(s): A4D173
, Q53GP6, Q75MK6, Q75MZ4, Q9UBZ3, Q9UED8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1998
Last modified: June 20, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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