O75529 (TAF5L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L

Alternative name(s):
PCAF-associated factor 65 beta
Short name=PAF65-beta

Gene names

Name:	TAF5L
Synonyms:	PAF65B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex.
Subunit structure	The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Ref.1
Subcellular location	Nucleus Ref.1 Ref.7.
Sequence similarities	Belongs to the WD repeat TAF5 family. Contains 6 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	histone H3 acetylation Inferred from direct assay Ref.7. Source: UniProtKB transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc
Cellular component	STAGA complex Inferred from direct assay Ref.7. Source: UniProtKB transcription factor TFTC complex Inferred from direct assay. Source: UniProtKB
Molecular function	sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc transcription coactivator activity Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75529-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75529-2)

The sequence of this isoform differs from the canonical sequence as follows:
     325-325: D → V
     326-589: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L		PRO_0000051261
Regions
	Repeat	266 – 305	40	WD 1
	Repeat	340 – 379	40	WD 2
	Repeat	382 – 421	40	WD 3
	Repeat	424 – 463	40	WD 4
	Repeat	466 – 505	40	WD 5
	Repeat	508 – 547	40	WD 6
Natural variations
	Alternative sequence	325	1	D → V in isoform 2.		VSP_010156
	Alternative sequence	326 – 589	264	Missing in isoform 2.		VSP_010157

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: BBF0A1CDD17EE22E	FASTA	589	66,155
	10 20 30 40 50 60 MKRVRTEQIQ MAVSCYLKRR QYVDSDGPLK QGLRLSQTAE EMAANLTVQS ESGCANIVSA 70 80 90 100 110 120 APCQAEPQQY EVQFGRLRNF LTDSDSQHSH EVMPLLYPLF VYLHLNLVQN SPKSTVESFY 130 140 150 160 170 180 SRFHGMFLQN ASQKDVIEQL QTTQTIQDIL SNFKLRAFLD NKYVVRLQED SYNYLIRYLQ 190 200 210 220 230 240 SDNNTALCKV LTLHIHLDVQ PAKRTDYQLY ASGSSSRSEN NGLEPPDMPS PILQNEAALE 250 260 270 280 290 300 VLQESIKRVK DGPPSLTTIC FYAFYNTEQL LNTAEISPDS KLLAAGFDNS CIKLWSLRSK 310 320 330 340 350 360 KLKSEPHQVD VSRIHLACDI LEEEDDEDDN AGTEMKILRG HCGPVYSTRF LADSSGLLSC 370 380 390 400 410 420 SEDMSIRYWD LGSFTNTVLY QGHAYPVWDL DISPYSLYFA SGSHDRTARL WSFDRTYPLR 430 440 450 460 470 480 IYAGHLADVD CVKFHPNSNY LATGSTDKTV RLWSAQQGNS VRLFTGHRGP VLSLAFSPNG 490 500 510 520 530 540 KYLASAGEDQ RLKLWDLASG TLYKELRGHT DNITSLTFSP DSGLIASASM DNSVRVWDIR 550 560 570 580 NTYCSAPADG SSSELVGVYT GQMSNVLSVQ FMACNLLLVT GITQENQEH
	Isoform 2 [UniParc]. Checksum: 509F5DFEF8BE696B	FASTA	325	36,991
	10 20 30 40 50 60 MKRVRTEQIQ MAVSCYLKRR QYVDSDGPLK QGLRLSQTAE EMAANLTVQS ESGCANIVSA 70 80 90 100 110 120 APCQAEPQQY EVQFGRLRNF LTDSDSQHSH EVMPLLYPLF VYLHLNLVQN SPKSTVESFY 130 140 150 160 170 180 SRFHGMFLQN ASQKDVIEQL QTTQTIQDIL SNFKLRAFLD NKYVVRLQED SYNYLIRYLQ 190 200 210 220 230 240 SDNNTALCKV LTLHIHLDVQ PAKRTDYQLY ASGSSSRSEN NGLEPPDMPS PILQNEAALE 250 260 270 280 290 300 VLQESIKRVK DGPPSLTTIC FYAFYNTEQL LNTAEISPDS KLLAAGFDNS CIKLWSLRSK 310 320 KLKSEPHQVD VSRIHLACDI LEEEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed: 9674425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 123-134 AND 167-177, SUBUNIT, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Eye.
[5]	"Characterization of 16 novel human genes showing high similarity to yeast sequences." Stanchi F., Bertocco E., Toppo S., Dioguardi R., Simionati B., Cannata N., Zimbello R., Lanfranchi G., Valle G. Yeast 18:69-80(2001) [PubMed: 11124703] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 330-589 (ISOFORM 1). Tissue: Brain.
[6]	"The TAFs in the HAT." Struhl K., Moqtaderi Z. Cell 94:1-4(1998) [PubMed: 9674419] [Abstract] Cited for: REVIEW, PCAF COMPLEX COMPOSITION.
[7]	"Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed: 11564863] [Abstract] Cited for: IDENTIFICATION IN THE STAGA COMPLEX, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[8]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed: 12601814] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069736 mRNA. Translation: AAC39906.1. AL121990 Genomic DNA. Translation: CAI22015.1. AL121990 Genomic DNA. Translation: CAI22016.1. CH471098 Genomic DNA. Translation: EAW69902.1. CH471098 Genomic DNA. Translation: EAW69903.1. BC041094 mRNA. Translation: AAH41094.1. AJ009770 mRNA. Translation: CAA08816.1.
IPI	IPI00004325. IPI00410088.
RefSeq	NP_001020418.1. NM_001025247.1. NP_055224.1. NM_014409.3.
UniGene	Hs.270621.
3D structure databases
ProteinModelPortal	O75529.
SMR	O75529. Positions 51-201, 260-584.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-28147N.
IntAct	O75529. 4 interactions.
STRING	O75529.
Proteomic databases
PRIDE	O75529.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000258281; ENSP00000258281; ENSG00000135801. ENST00000366676; ENSP00000355636; ENSG00000135801.
GeneID	27097.
KEGG	hsa:27097.
UCSC	uc001htq.1. human.
Organism-specific databases
CTD	27097.
GeneCards	GC01M229728.
HGNC	HGNC:17304. TAF5L.
neXtProt	NX_O75529.
PharmGKB	PA38223.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14045.
GeneTree	ENSGT00600000084184.
HOGENOM	HBG559818.
HOVERGEN	HBG050226.
InParanoid	O75529.
OMA	LWSTQQG.
OrthoDB	EOG4NCMCF.
PhylomeDB	O75529.
Gene expression databases
ArrayExpress	O75529.
Bgee	O75529.
CleanEx	HS_TAF5L.
Genevestigator	O75529.
GermOnline	ENSG00000135801. Homo sapiens.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR007582. TFIID-su_WD40-assoc_reg. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K03130.
Pfam	PF04494. TFIID_90kDa. 1 hit. PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	49741.

Entry information

Entry name

TAF5L_HUMAN

Accession

Primary (citable) accession number: O75529
Secondary accession number(s): Q5TDI5, Q5TDI6, Q8IW31

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.