Reviewed,
UniProtKB/Swiss-Prot O75528 (TAD3L_HUMAN)
Last modified
June 16, 2009.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Transcriptional adapter 3-like Short name=ADA3-like protein Alternative name(s): ADA3 homolog Short name=hADA3 STAF54 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 432 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Ref.7 |
| Subunit structure | The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Interacts directly with TADA2L and PCAF and also with the high-risk HPV oncoprotein E6. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Component of the TFTC-HAT complex. Ref.7 Ref.1 Ref.6 |
| Subcellular location | |
| Tissue specificity | Ubiquitously expressed. |
| Sequence similarities | Belongs to the NGG1 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing |
| Domain | Coiled coil |
| Gene Ontology (GO) | |
| Biological process | histone H3 acetylation Ref.5 Inferred from direct assay. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Ref.1Traceable author statement. Source: ProtInc transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | STAGA complex Ref.5 Inferred from direct assay. Source: UniProtKB transcription factor TFTC complexInferred from direct assay. Source: UniProtKB |
| Molecular function | transcription coactivator activity Ref.5 Inferred from direct assay. Source: UniProtKB transcription factor activity Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PIAS4 | Q8N2W9 | 1 | EBI-473249,EBI-473160 | |
| USP5 | P45974 | 1 | EBI-473249,EBI-741277 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: O75528-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O75528-2) The sequence of this isoform differs from the canonical sequence as follows: 370-432: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 432 | 432 | Transcriptional adapter 3-like | PRO_0000072416 | |||||
Regions | |||||||||
| Coiled coil | 40 – 69 | 30 | Potential | ||||||
| Coiled coil | 367 – 407 | 41 | Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 370 – 432 | 63 | Missing in isoform 2. | VSP_009739 | |||||
Experimental info | |||||||||
| Sequence conflict | 168 | 1 | E → G in AK000228. Ref.3 | ||||||
Sequences
| ||||||||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed: 9674425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH PCAF; TAF5L; TAF6L; TAF9; TAF10 AND TAF12. |
| [2] | "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A.Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon mucosa. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Muscle and Pancreas. |
| [5] | "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed: 11564863] [Abstract] Cited for: IDENTIFICATION IN THE STAGA COMPLEX, SUBCELLULAR LOCATION, MASS SPECTROMETRY. |
| [6] | "Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3." Kumar A., Zhao Y., Meng G., Zeng M., Srinivasan S., Delmolino L.M., Gao Q., Dimri G., Weber G.F., Wazer D.E., Band H., Band V. Mol. Cell. Biol. 22:5801-5812(2002) [PubMed: 12138191] [Abstract] Cited for: INTERACTION WITH TP53 AND THE HIGH-RISK HPV ONCOPROTEIN E6. |
| [7] | "hADA3 is required for p53 activity." Wang T., Kobayashi T., Takimoto R., Denes A.E., Snyder E.L., el-Deiry W.S., Brachmann R.K. EMBO J. 20:6404-6413(2001) [PubMed: 11707411] [Abstract] Cited for: FUNCTION, INTERACTION WITH TP53. |
| [8] | "The TAFs in the HAT." Struhl K., Moqtaderi Z. Cell 94:1-4(1998) [PubMed: 9674419] [Abstract] Cited for: REVIEW, PCAF COMPLEX COMPOSITION. |
| [9] | "Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed: 12601814] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF069733 mRNA. Translation: AAC39903.1. AL117487 mRNA. Translation: CAB55957.1. AK000228 mRNA. No translation available. BC009240 mRNA. Translation: AAH09240.1. BC013433 mRNA. Translation: AAH13433.1. | |
| IPI | IPI00220486. IPI00375534. |
| PIR | T17267. |
| RefSeq | NP_006345.1. NP_597814.1. |
| UniGene | Hs.386390 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O75528. 17 interactions. |
PTM databases | |
| PhosphoSite | O75528. |
Proteomic databases | |
| PRIDE | O75528. |
Genome annotation databases | |
| Ensembl | ENSG00000171148. Homo sapiens. [Contig view] |
| GeneID | 10474. |
| KEGG | hsa:10474. |
Organism-specific databases | |
| GeneCards | GC03M009796. |
| HGNC | HGNC:19422. TADA3L. |
| MIM | 602945. gene. |
| PharmGKB | PA134910395. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | O75528. |
| HOVERGEN | O75528. |
| OMA | O75528. GCPFGPL. |
Gene expression databases | |
| ArrayExpress | O75528. |
| Bgee | O75528. |
| CleanEx | HS_TADA3L. |
| GermOnline | ENSG00000171148. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR019340. Histone_AcTrfase_su3. [Graphical view] |
| Pfam | PF10198. Ada3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 39724. |
| SOURCE | Search... |
Entry information
| Entry name | TAD3L_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O75528 Secondary accession number(s): Q9UFS2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


