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Protein

Transcriptional adapter 3

Gene

TADA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.2 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor binding Source: UniProtKB
  • ligand-dependent nuclear receptor transcription coactivator activity Source: Ensembl
  • protein domain specific binding Source: UniProtKB
  • thiol-dependent ubiquitinyl hydrolase activity Source: Reactome
  • transcription coactivator activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • histone H3 acetylation Source: UniProtKB
  • histone H4 acetylation Source: GO_Central
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • mitotic cell cycle Source: Ensembl
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • protein deubiquitination Source: Reactome
  • regulation of histone deacetylation Source: Ensembl
  • regulation of protein phosphorylation Source: Ensembl
  • regulation of protein stability Source: Ensembl
  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
  • regulation of tubulin deacetylation Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214847. HATs acetylate histones.
R-HSA-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional adapter 3
Alternative name(s):
ADA3 homolog
Short name:
hADA3
STAF54
Transcriptional adapter 3-like
Short name:
ADA3-like protein
Gene namesi
Name:TADA3
Synonyms:ADA3, TADA3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:19422. TADA3.

Subcellular locationi

GO - Cellular componenti

  • Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
  • intracellular Source: LIFEdb
  • mitotic spindle Source: Ensembl
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • SAGA complex Source: GO_Central
  • STAGA complex Source: UniProtKB
  • transcription factor TFTC complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10474.
OpenTargetsiENSG00000171148.
PharmGKBiPA165698494.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000724161 – 432Transcriptional adapter 3Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei280PhosphoserineCombined sources1
Modified residuei298PhosphoserineCombined sources1
Modified residuei418N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO75528.
MaxQBiO75528.
PaxDbiO75528.
PeptideAtlasiO75528.
PRIDEiO75528.

PTM databases

iPTMnetiO75528.
PhosphoSitePlusiO75528.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSG00000171148.
CleanExiHS_TADA3L.
ExpressionAtlasiO75528. baseline and differential.
GenevisibleiO75528. HS.

Organism-specific databases

HPAiHPA042250.

Interactioni

Subunit structurei

The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Interacts directly with TADA2L and PCAF and also with the high-risk HPV oncoprotein E6. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Component of the TFTC-HAT complex. Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1.6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ligand-dependent nuclear receptor binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115737. 67 interactors.
IntActiO75528. 34 interactors.
MINTiMINT-221730.
STRINGi9606.ENSP00000307684.

Structurei

3D structure databases

ProteinModelPortaliO75528.
SMRiO75528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili40 – 69Sequence analysisAdd BLAST30
Coiled coili367 – 407Sequence analysisAdd BLAST41

Sequence similaritiesi

Belongs to the NGG1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4191. Eukaryota.
ENOG410XQUH. LUCA.
GeneTreeiENSGT00390000008947.
HOGENOMiHOG000007362.
HOVERGENiHBG055283.
InParanoidiO75528.
KOiK11315.
OMAiVPGSPPC.
OrthoDBiEOG091G0DWR.
PhylomeDBiO75528.
TreeFamiTF323397.

Family and domain databases

InterProiView protein in InterPro
IPR019340. Histone_AcTrfase_su3.
PfamiView protein in Pfam
PF10198. Ada3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75528-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL
60 70 80 90 100
ETLLSSASRR LRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK
110 120 130 140 150
PKKQKLEGKA GHGPGPGPGR PKSKNLQPKI QEYEFTDDPI DVPRIPKNDA
160 170 180 190 200
PNRFWASVEP YCADITSEEV RTLEELLKPP EDEAEHYKIP PLGKHYSQRW
210 220 230 240 250
AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDAL LKKSEAQHEQ
260 270 280 290 300
PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPR
310 320 330 340 350
NQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ
360 370 380 390 400
AELKALSAHN RTKKHDLLRL AKEEVSRQEL RQRVRMADNE VMDAFRKIMA
410 420 430
ARQKKRTPTK KEKDQAWKTL KERESILKLL DG
Length:432
Mass (Da):48,902
Last modified:November 1, 1998 - v1
Checksum:iC86153CFA83F9226
GO
Isoform 2 (identifier: O75528-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-432: Missing.

Show »
Length:369
Mass (Da):41,393
Checksum:i782715A6300779A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168E → G in AK000228 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009739370 – 432Missing in isoform 2. 3 PublicationsAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069733 mRNA. Translation: AAC39903.1.
AL117487 mRNA. Translation: CAB55957.1.
AK000228 mRNA. No translation available.
CR533543 mRNA. Translation: CAG38574.1.
CH471055 Genomic DNA. Translation: EAW63994.1.
BC009240 mRNA. Translation: AAH09240.1.
BC013433 mRNA. Translation: AAH13433.1.
CCDSiCCDS2583.1. [O75528-1]
CCDS2584.1. [O75528-2]
PIRiT17267.
RefSeqiNP_001265199.1. NM_001278270.1. [O75528-1]
NP_006345.1. NM_006354.3. [O75528-1]
NP_597814.1. NM_133480.2. [O75528-2]
UniGeneiHs.386390.

Genome annotation databases

EnsembliENST00000301964; ENSP00000307684; ENSG00000171148. [O75528-1]
ENST00000343450; ENSP00000343649; ENSG00000171148. [O75528-2]
ENST00000440161; ENSP00000393471; ENSG00000171148. [O75528-1]
GeneIDi10474.
KEGGihsa:10474.
UCSCiuc003bsx.3. human. [O75528-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTADA3_HUMAN
AccessioniPrimary (citable) accession number: O75528
Secondary accession number(s): Q6FI83, Q9UFS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: November 1, 1998
Last modified: June 7, 2017
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families