O75528 (TADA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional adapter 3

Alternative name(s):
ADA3 homolog
Short name=hADA3
STAF54
Transcriptional adapter 3-like
Short name=ADA3-like protein

Gene names

Name:	TADA3
Synonyms:	ADA3, TADA3L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Ref.9 Ref.12
Subunit structure	The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Interacts directly with TADA2L and PCAF and also with the high-risk HPV oncoprotein E6. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Component of the TFTC-HAT complex. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Ref.1 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12
Subcellular location	Nucleus Ref.1 Ref.7.
Tissue specificity	Ubiquitously expressed.
Sequence similarities	Belongs to the NGG1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H3 acetylation Inferred from direct assay Ref.7. Source: UniProtKB intracellular estrogen receptor signaling pathway Traceable author statement PubMed 20413580. Source: UniProtKB mitosis Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 20413580. Source: UniProtKB regulation of histone deacetylation Inferred from electronic annotation. Source: Compara regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara regulation of protein stability Inferred from electronic annotation. Source: Compara regulation of transcription from RNA polymerase II promoter Traceable author statement Ref.1. Source: ProtInc regulation of tubulin deacetylation Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Ada2/Gcn5/Ada3 transcription activator complex Inferred from direct assay PubMed 18838386. Source: BHF-UCL STAGA complex Inferred from direct assay Ref.7. Source: UniProtKB mitotic spindle Inferred from electronic annotation. Source: Compara transcription factor TFTC complex Inferred from direct assay PubMed 10373431. Source: UniProtKB
Molecular_function	ligand-dependent nuclear receptor transcription coactivator activity Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Traceable author statement Ref.1. Source: ProtInc transcription coactivator activity Inferred from direct assay Ref.7PubMed 20413580. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PIAS4	Q8N2W9	2	EBI-473249,EBI-473160
USP5	P45974	2	EBI-473249,EBI-741277

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75528-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75528-2)

The sequence of this isoform differs from the canonical sequence as follows:
     370-432: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Transcriptional adapter 3		PRO_0000072416
Regions
	Coiled coil	40 – 69	30	Potential
	Coiled coil	367 – 407	41	Potential
Amino acid modifications
	Modified residue	418	1	N6-acetyllysine Ref.13
Natural variations
	Alternative sequence	370 – 432	63	Missing in isoform 2.		VSP_009739
Experimental info
	Sequence conflict	168	1	E → G in AK000228. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C86153CFA83F9226	FASTA	432	48,902
	10 20 30 40 50 60 MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL ETLLSSASRR 70 80 90 100 110 120 LRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK PKKQKLEGKA GHGPGPGPGR 130 140 150 160 170 180 PKSKNLQPKI QEYEFTDDPI DVPRIPKNDA PNRFWASVEP YCADITSEEV RTLEELLKPP 190 200 210 220 230 240 EDEAEHYKIP PLGKHYSQRW AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDAL 250 260 270 280 290 300 LKKSEAQHEQ PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPR 310 320 330 340 350 360 NQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ AELKALSAHN 370 380 390 400 410 420 RTKKHDLLRL AKEEVSRQEL RQRVRMADNE VMDAFRKIMA ARQKKRTPTK KEKDQAWKTL 430 KERESILKLL DG
	Isoform 2 [UniParc]. Checksum: 782715A6300779A4	FASTA	369	41,393
	10 20 30 40 50 60 MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL ETLLSSASRR 70 80 90 100 110 120 LRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK PKKQKLEGKA GHGPGPGPGR 130 140 150 160 170 180 PKSKNLQPKI QEYEFTDDPI DVPRIPKNDA PNRFWASVEP YCADITSEEV RTLEELLKPP 190 200 210 220 230 240 EDEAEHYKIP PLGKHYSQRW AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDAL 250 260 270 280 290 300 LKKSEAQHEQ PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPR 310 320 330 340 350 360 NQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ AELKALSAHN RTKKHDLLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH PCAF; TAF5L; TAF6L; TAF9; TAF10 AND TAF12.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon mucosa.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Muscle and Pancreas.
[7]	"Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE STAGA COMPLEX, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[8]	"Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3." Kumar A., Zhao Y., Meng G., Zeng M., Srinivasan S., Delmolino L.M., Gao Q., Dimri G., Weber G.F., Wazer D.E., Band H., Band V. Mol. Cell. Biol. 22:5801-5812(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53 AND THE HIGH-RISK HPV ONCOPROTEIN E6.
[9]	"hADA3 is required for p53 activity." Wang T., Kobayashi T., Takimoto R., Denes A.E., Snyder E.L., el-Deiry W.S., Brachmann R.K. EMBO J. 20:6404-6413(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TP53.
[10]	"The TAFs in the HAT." Struhl K., Moqtaderi Z. Cell 94:1-4(1998) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW, PCAF COMPLEX COMPOSITION.
[11]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX.
[12]	"The double-histone-acetyltransferase complex ATAC is essential for mammalian development." Guelman S., Kozuka K., Mao Y., Pham V., Solloway M.J., Wang J., Wu J., Lill J.R., Zha J. Mol. Cell. Biol. 29:1176-1188(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN ATAC COMPLEX.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-418, MASS SPECTROMETRY.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069733 mRNA. Translation: AAC39903.1. AL117487 mRNA. Translation: CAB55957.1. AK000228 mRNA. No translation available. CR533543 mRNA. Translation: CAG38574.1. CH471055 Genomic DNA. Translation: EAW63994.1. BC009240 mRNA. Translation: AAH09240.1. BC013433 mRNA. Translation: AAH13433.1.
IPI	IPI00220486. IPI00375534.
PIR	T17267.
RefSeq	NP_006345.1. NM_006354.2. NP_597814.1. NM_133480.1.
UniGene	Hs.386390.
3D structure databases
ProteinModelPortal	O75528.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75528. 19 interactions.
MINT	MINT-221730.
STRING	9606.ENSP00000307684.
PTM databases
PhosphoSite	O75528.
Proteomic databases
PaxDb	O75528.
PRIDE	O75528.
Protocols and materials databases
DNASU	10474.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301964; ENSP00000307684; ENSG00000171148. ENST00000343450; ENSP00000343649; ENSG00000171148. ENST00000440161; ENSP00000393471; ENSG00000171148.
GeneID	10474.
KEGG	hsa:10474.
UCSC	uc003bsw.1. human.
Organism-specific databases
CTD	10474.
GeneCards	GC03M009797.
HGNC	HGNC:19422. TADA3.
HPA	HPA042250.
MIM	602945. gene.
neXtProt	NX_O75528.
PharmGKB	PA165698494.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG265778.
HOGENOM	HOG000007362.
HOVERGEN	HBG055283.
InParanoid	O75528.
KO	K11315.
OMA	QDKKGDK.
OrthoDB	EOG4ZW5B5.
PhylomeDB	O75528.
Gene expression databases
ArrayExpress	O75528.
Bgee	O75528.
CleanEx	HS_TADA3L.
Genevestigator	O75528.
GermOnline	ENSG00000171148. Homo sapiens.
Family and domain databases
InterPro	IPR019340. Histone_AcTrfase_su3. [Graphical view]
Pfam	PF10198. Ada3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TADA3. human.
GenomeRNAi	10474.
NextBio	39724.
SOURCE	Search...

Entry information

Entry name

TADA3_HUMAN

Accession

Primary (citable) accession number: O75528
Secondary accession number(s): Q6FI83, Q9UFS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	November 1, 1998
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.