Reviewed, UniProtKB/Swiss-Prot O75528 (TAD3L_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcriptional adapter 3-like
      Short name=ADA3-like protein
Alternative name(s):
    ADA3 homolog
      Short name=hADA3
    STAF54

Gene names

Name:	TADA3L
Synonyms:	ADA3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Ref.7
Subunit structure	The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Interacts directly with TADA2L and PCAF and also with the high-risk HPV oncoprotein E6. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Component of the TFTC-HAT complex. Ref.7 Ref.1 Ref.6
Subcellular location	Nucleus. Ref.1 Ref.5
Tissue specificity	Ubiquitously expressed.
Sequence similarities	Belongs to the NGG1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Gene Ontology (GO)
Biological process	histone H3 acetylation Ref.5 Inferred from direct assay. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	STAGA complex Ref.5 Inferred from direct assay. Source: UniProtKB transcription factor TFTC complex Inferred from direct assay. Source: UniProtKB
Molecular function	transcription coactivator activity Ref.5 Inferred from direct assay. Source: UniProtKB transcription factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PIAS4	Q8N2W9	1	EBI-473249,EBI-473160
USP5	P45974	1	EBI-473249,EBI-741277

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75528-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75528-2)

The sequence of this isoform differs from the canonical sequence as follows:
     370-432: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Transcriptional adapter 3-like		PRO_0000072416
Regions
	Coiled coil	40 – 69	30	Potential
	Coiled coil	367 – 407	41	Potential
Natural variations
	Alternative sequence	370 – 432	63	Missing in isoform 2.		VSP_009739
Experimental info
	Sequence conflict	168	1	E → G in AK000228. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C86153CFA83F9226	FASTA	432	48,902
	10 20 30 40 50 60 MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL ETLLSSASRR 70 80 90 100 110 120 LRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK PKKQKLEGKA GHGPGPGPGR 130 140 150 160 170 180 PKSKNLQPKI QEYEFTDDPI DVPRIPKNDA PNRFWASVEP YCADITSEEV RTLEELLKPP 190 200 210 220 230 240 EDEAEHYKIP PLGKHYSQRW AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDAL 250 260 270 280 290 300 LKKSEAQHEQ PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPR 310 320 330 340 350 360 NQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ AELKALSAHN 370 380 390 400 410 420 RTKKHDLLRL AKEEVSRQEL RQRVRMADNE VMDAFRKIMA ARQKKRTPTK KEKDQAWKTL 430 KERESILKLL DG
	Isoform 2. Checksum: 782715A6300779A4	FASTA	369	41,393
	10 20 30 40 50 60 MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL ETLLSSASRR 70 80 90 100 110 120 LRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK PKKQKLEGKA GHGPGPGPGR 130 140 150 160 170 180 PKSKNLQPKI QEYEFTDDPI DVPRIPKNDA PNRFWASVEP YCADITSEEV RTLEELLKPP 190 200 210 220 230 240 EDEAEHYKIP PLGKHYSQRW AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDAL 250 260 270 280 290 300 LKKSEAQHEQ PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPR 310 320 330 340 350 360 NQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ AELKALSAHN RTKKHDLLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed: 9674425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH PCAF; TAF5L; TAF6L; TAF9; TAF10 AND TAF12.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon mucosa.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Muscle and Pancreas.
[5]	"Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo." Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G. Mol. Cell. Biol. 21:6782-6795(2001) [PubMed: 11564863] [Abstract] Cited for: IDENTIFICATION IN THE STAGA COMPLEX, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[6]	"Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3." Kumar A., Zhao Y., Meng G., Zeng M., Srinivasan S., Delmolino L.M., Gao Q., Dimri G., Weber G.F., Wazer D.E., Band H., Band V. Mol. Cell. Biol. 22:5801-5812(2002) [PubMed: 12138191] [Abstract] Cited for: INTERACTION WITH TP53 AND THE HIGH-RISK HPV ONCOPROTEIN E6.
[7]	"hADA3 is required for p53 activity." Wang T., Kobayashi T., Takimoto R., Denes A.E., Snyder E.L., el-Deiry W.S., Brachmann R.K. EMBO J. 20:6404-6413(2001) [PubMed: 11707411] [Abstract] Cited for: FUNCTION, INTERACTION WITH TP53.
[8]	"The TAFs in the HAT." Struhl K., Moqtaderi Z. Cell 94:1-4(1998) [PubMed: 9674419] [Abstract] Cited for: REVIEW, PCAF COMPLEX COMPOSITION.
[9]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed: 12601814] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF069733 mRNA. Translation: AAC39903.1. AL117487 mRNA. Translation: CAB55957.1. AK000228 mRNA. No translation available. BC009240 mRNA. Translation: AAH09240.1. BC013433 mRNA. Translation: AAH13433.1.
IPI	IPI00220486. IPI00375534.
PIR	T17267.
RefSeq	NP_006345.1. NP_597814.1.
UniGene	Hs.386390
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O75528. 17 interactions.
PTM databases
PhosphoSite	O75528.
Proteomic databases
PRIDE	O75528.
Genome annotation databases
Ensembl	ENSG00000171148. Homo sapiens. [Contig view]
GeneID	10474.
KEGG	hsa:10474.
Organism-specific databases
GeneCards	GC03M009796.
HGNC	HGNC:19422. TADA3L.
MIM	602945. gene.
PharmGKB	PA134910395.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75528.
HOVERGEN	O75528.
OMA	O75528. GCPFGPL.
Gene expression databases
ArrayExpress	O75528.
Bgee	O75528.
CleanEx	HS_TADA3L.
GermOnline	ENSG00000171148. Homo sapiens.
Family and domain databases
InterPro	IPR019340. Histone_AcTrfase_su3. [Graphical view]
Pfam	PF10198. Ada3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	39724.
SOURCE	Search...

Entry information

Entry name

TAD3L_HUMAN

Accession

Primary (citable) accession number: O75528
Secondary accession number(s): Q9UFS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)