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O75528

- TADA3_HUMAN

UniProt

O75528 - TADA3_HUMAN

Protein

Transcriptional adapter 3

Gene

TADA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.2 Publications

    GO - Molecular functioni

    1. ligand-dependent nuclear receptor binding Source: UniProtKB
    2. ligand-dependent nuclear receptor transcription coactivator activity Source: Ensembl
    3. protein binding Source: IntAct
    4. protein domain specific binding Source: UniProtKB
    5. sequence-specific DNA binding transcription factor activity Source: ProtInc
    6. transcription coactivator activity Source: UniProtKB

    GO - Biological processi

    1. chromatin organization Source: Reactome
    2. histone H3 acetylation Source: UniProtKB
    3. intracellular estrogen receptor signaling pathway Source: UniProtKB
    4. mitotic nuclear division Source: Ensembl
    5. positive regulation of gene expression Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. regulation of histone deacetylation Source: Ensembl
    8. regulation of protein phosphorylation Source: Ensembl
    9. regulation of protein stability Source: Ensembl
    10. regulation of transcription from RNA polymerase II promoter Source: ProtInc
    11. regulation of tubulin deacetylation Source: Ensembl
    12. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_172610. HATs acetylate histones.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional adapter 3
    Alternative name(s):
    ADA3 homolog
    Short name:
    hADA3
    STAF54
    Transcriptional adapter 3-like
    Short name:
    ADA3-like protein
    Gene namesi
    Name:TADA3
    Synonyms:ADA3, TADA3L
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:19422. TADA3.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. Ada2/Gcn5/Ada3 transcription activator complex Source: BHF-UCL
    2. intracellular Source: LIFEdb
    3. mitotic spindle Source: Ensembl
    4. nucleus Source: HPA
    5. STAGA complex Source: UniProtKB
    6. transcription factor TFTC complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA165698494.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 432432Transcriptional adapter 3PRO_0000072416Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei418 – 4181N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO75528.
    PaxDbiO75528.
    PRIDEiO75528.

    PTM databases

    PhosphoSiteiO75528.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Gene expression databases

    ArrayExpressiO75528.
    BgeeiO75528.
    CleanExiHS_TADA3L.
    GenevestigatoriO75528.

    Organism-specific databases

    HPAiHPA042250.

    Interactioni

    Subunit structurei

    The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Interacts directly with TADA2L and PCAF and also with the high-risk HPV oncoprotein E6. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Component of the TFTC-HAT complex. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PIAS4Q8N2W92EBI-473249,EBI-473160
    USP5P459742EBI-473249,EBI-741277

    Protein-protein interaction databases

    BioGridi115737. 40 interactions.
    IntActiO75528. 19 interactions.
    MINTiMINT-221730.
    STRINGi9606.ENSP00000307684.

    Structurei

    3D structure databases

    ProteinModelPortaliO75528.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili40 – 6930Sequence AnalysisAdd
    BLAST
    Coiled coili367 – 40741Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NGG1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG265778.
    HOGENOMiHOG000007362.
    HOVERGENiHBG055283.
    InParanoidiO75528.
    KOiK11315.
    OMAiQDKKGDK.
    OrthoDBiEOG79PJP8.
    PhylomeDBiO75528.
    TreeFamiTF323397.

    Family and domain databases

    InterProiIPR019340. Histone_AcTrfase_su3.
    [Graphical view]
    PfamiPF10198. Ada3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O75528-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSELKDCPLQ FHDFKSVDHL KVCPRYTAVL ARSEDDGIGI EELDTLQLEL    50
    ETLLSSASRR LRVLEAETQI LTDWQDKKGD RRFLKLGRDH ELGAPPKHGK 100
    PKKQKLEGKA GHGPGPGPGR PKSKNLQPKI QEYEFTDDPI DVPRIPKNDA 150
    PNRFWASVEP YCADITSEEV RTLEELLKPP EDEAEHYKIP PLGKHYSQRW 200
    AQEDLLEEQK DGARAAAVAD KKKGLMGPLT ELDTKDVDAL LKKSEAQHEQ 250
    PEDGCPFGAL TQRLLQALVE ENIISPMEDS PIPDMSGKES GADGASTSPR 300
    NQNKPFSVPH TKSLESRIKE ELIAQGLLES EDRPAEDSED EVLAELRKRQ 350
    AELKALSAHN RTKKHDLLRL AKEEVSRQEL RQRVRMADNE VMDAFRKIMA 400
    ARQKKRTPTK KEKDQAWKTL KERESILKLL DG 432
    Length:432
    Mass (Da):48,902
    Last modified:November 1, 1998 - v1
    Checksum:iC86153CFA83F9226
    GO
    Isoform 2 (identifier: O75528-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         370-432: Missing.

    Show »
    Length:369
    Mass (Da):41,393
    Checksum:i782715A6300779A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti168 – 1681E → G in AK000228. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei370 – 43263Missing in isoform 2. 3 PublicationsVSP_009739Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069733 mRNA. Translation: AAC39903.1.
    AL117487 mRNA. Translation: CAB55957.1.
    AK000228 mRNA. No translation available.
    CR533543 mRNA. Translation: CAG38574.1.
    CH471055 Genomic DNA. Translation: EAW63994.1.
    BC009240 mRNA. Translation: AAH09240.1.
    BC013433 mRNA. Translation: AAH13433.1.
    CCDSiCCDS2583.1. [O75528-1]
    CCDS2584.1. [O75528-2]
    PIRiT17267.
    RefSeqiNP_001265199.1. NM_001278270.1. [O75528-1]
    NP_006345.1. NM_006354.3. [O75528-1]
    NP_597814.1. NM_133480.2. [O75528-2]
    UniGeneiHs.386390.

    Genome annotation databases

    EnsembliENST00000301964; ENSP00000307684; ENSG00000171148. [O75528-1]
    ENST00000343450; ENSP00000343649; ENSG00000171148. [O75528-2]
    ENST00000440161; ENSP00000393471; ENSG00000171148. [O75528-1]
    GeneIDi10474.
    KEGGihsa:10474.
    UCSCiuc003bsw.2. human. [O75528-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069733 mRNA. Translation: AAC39903.1 .
    AL117487 mRNA. Translation: CAB55957.1 .
    AK000228 mRNA. No translation available.
    CR533543 mRNA. Translation: CAG38574.1 .
    CH471055 Genomic DNA. Translation: EAW63994.1 .
    BC009240 mRNA. Translation: AAH09240.1 .
    BC013433 mRNA. Translation: AAH13433.1 .
    CCDSi CCDS2583.1. [O75528-1 ]
    CCDS2584.1. [O75528-2 ]
    PIRi T17267.
    RefSeqi NP_001265199.1. NM_001278270.1. [O75528-1 ]
    NP_006345.1. NM_006354.3. [O75528-1 ]
    NP_597814.1. NM_133480.2. [O75528-2 ]
    UniGenei Hs.386390.

    3D structure databases

    ProteinModelPortali O75528.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115737. 40 interactions.
    IntActi O75528. 19 interactions.
    MINTi MINT-221730.
    STRINGi 9606.ENSP00000307684.

    PTM databases

    PhosphoSitei O75528.

    Proteomic databases

    MaxQBi O75528.
    PaxDbi O75528.
    PRIDEi O75528.

    Protocols and materials databases

    DNASUi 10474.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000301964 ; ENSP00000307684 ; ENSG00000171148 . [O75528-1 ]
    ENST00000343450 ; ENSP00000343649 ; ENSG00000171148 . [O75528-2 ]
    ENST00000440161 ; ENSP00000393471 ; ENSG00000171148 . [O75528-1 ]
    GeneIDi 10474.
    KEGGi hsa:10474.
    UCSCi uc003bsw.2. human. [O75528-1 ]

    Organism-specific databases

    CTDi 10474.
    GeneCardsi GC03M009797.
    HGNCi HGNC:19422. TADA3.
    HPAi HPA042250.
    MIMi 602945. gene.
    neXtProti NX_O75528.
    PharmGKBi PA165698494.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG265778.
    HOGENOMi HOG000007362.
    HOVERGENi HBG055283.
    InParanoidi O75528.
    KOi K11315.
    OMAi QDKKGDK.
    OrthoDBi EOG79PJP8.
    PhylomeDBi O75528.
    TreeFami TF323397.

    Enzyme and pathway databases

    Reactomei REACT_172610. HATs acetylate histones.

    Miscellaneous databases

    ChiTaRSi TADA3. human.
    GeneWikii TADA3L.
    GenomeRNAii 10474.
    NextBioi 39724.
    PROi O75528.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75528.
    Bgeei O75528.
    CleanExi HS_TADA3L.
    Genevestigatori O75528.

    Family and domain databases

    InterProi IPR019340. Histone_AcTrfase_su3.
    [Graphical view ]
    Pfami PF10198. Ada3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH PCAF; TAF5L; TAF6L; TAF9; TAF10 AND TAF12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Testis.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon mucosa.
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Muscle and Pancreas.
    7. "Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo."
      Martinez E., Palhan V.B., Tjernberg A., Lymar E.S., Gamper A.M., Kundu T.K., Chait B.T., Roeder R.G.
      Mol. Cell. Biol. 21:6782-6795(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE STAGA COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3."
      Kumar A., Zhao Y., Meng G., Zeng M., Srinivasan S., Delmolino L.M., Gao Q., Dimri G., Weber G.F., Wazer D.E., Band H., Band V.
      Mol. Cell. Biol. 22:5801-5812(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TP53 AND THE HIGH-RISK HPV ONCOPROTEIN E6.
    9. Cited for: FUNCTION, INTERACTION WITH TP53.
    10. Cited for: REVIEW, PCAF COMPLEX COMPOSITION.
    11. "Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis."
      Cavusoglu N., Brand M., Tora L., van Dorsselaer A.
      Proteomics 3:217-223(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX.
    12. "The double-histone-acetyltransferase complex ATAC is essential for mammalian development."
      Guelman S., Kozuka K., Mao Y., Pham V., Solloway M.J., Wang J., Wu J., Lill J.R., Zha J.
      Mol. Cell. Biol. 29:1176-1188(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN ATAC COMPLEX.
    13. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-418, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiTADA3_HUMAN
    AccessioniPrimary (citable) accession number: O75528
    Secondary accession number(s): Q6FI83, Q9UFS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3