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Protein

Serine/arginine-rich splicing factor 10

Gene

SRSF10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing (PubMed:11684676, PubMed:12419250, PubMed:14765198). Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70 (PubMed:14765198). Required for splicing repression in M-phase cells and after heat shock (PubMed:14765198). Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator (PubMed:12419250).4 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • RS domain binding Source: UniProtKB
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

  • cytosolic transport Source: Ensembl
  • mRNA export from nucleus Source: UniProtKB
  • mRNA splice site selection Source: UniProtKB
  • mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • RNA splicing, via transesterification reactions Source: UniProtKB
  • spliceosomal tri-snRNP complex assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:G66-33709-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 10
Alternative name(s):
40 kDa SR-repressor protein
Short name:
SRrp40
FUS-interacting serine-arginine-rich protein 1
Splicing factor SRp38
Splicing factor, arginine/serine-rich 13A
TLS-associated protein with Ser-Arg repeats
Short name:
TASR
Short name:
TLS-associated protein with SR repeats
TLS-associated serine-arginine protein
Short name:
TLS-associated SR protein
Gene namesi
Name:SRSF10
Synonyms:FUSIP1, FUSIP2, SFRS13A, TASR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:16713. SRSF10.

Subcellular locationi

GO - Cellular componenti

  • axon terminus Source: Ensembl
  • cytoplasm Source: UniProtKB
  • cytosol Source: GOC
  • dendrite Source: Ensembl
  • neuronal cell body Source: Ensembl
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10772.
OpenTargetsiENSG00000188529.
PharmGKBiPA28427.

Polymorphism and mutation databases

BioMutaiSRSF10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815931 – 262Serine/arginine-rich splicing factor 10Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineCombined sources1
Modified residuei106PhosphoserineCombined sources1
Modified residuei108PhosphoserineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Modified residuei158PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Isoform 4 (identifier: O75494-4)
Modified residuei158PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Isoform 3 (identifier: O75494-3)
Modified residuei168PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Fully dephosphorylated in mitosis and partially dephosphorylated on heat shock.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75494.
MaxQBiO75494.
PaxDbiO75494.
PeptideAtlasiO75494.
PRIDEiO75494.
TopDownProteomicsiO75494-1. [O75494-1]
O75494-2. [O75494-2]
O75494-3. [O75494-3]
O75494-4. [O75494-4]
O75494-5. [O75494-5]

PTM databases

iPTMnetiO75494.
PhosphoSitePlusiO75494.
SwissPalmiO75494.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSG00000188529.
ExpressionAtlasiO75494. baseline and differential.
GenevisibleiO75494. HS.

Organism-specific databases

HPAiHPA053805.
HPA053831.

Interactioni

Subunit structurei

The phosphorylated but not the dephosphorylated form interacts with TRA2B/SFRS10 (PubMed:14765198). The dephosphorylated form interacts with SNRNP70 (PubMed:14765198). Isoform 1 interacts with FUS C-terminus (PubMed:9774382). Isoform 3 interacts with FUS C-terminus (PubMed:9774382). Interacts with YTHDC1, leading to inhibit RNA-binding activity of SRSF10 (PubMed:26876937).3 Publications

GO - Molecular functioni

  • RS domain binding Source: UniProtKB
  • unfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115990. 79 interactors.
IntActiO75494. 28 interactors.
MINTiMINT-5002282.
STRINGi9606.ENSP00000420195.

Structurei

3D structure databases

ProteinModelPortaliO75494.
SMRiO75494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 88RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi106 – 260Arg/Ser-rich (RS domain)Add BLAST155

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104403.
HOGENOMiHOG000276233.
HOVERGENiHBG107480.
InParanoidiO75494.
KOiK12900.
OMAiFAYVQYT.
OrthoDBiEOG091G13K0.
PhylomeDBiO75494.
TreeFamiTF351864.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75494-1) [UniParc]FASTAAdd to basket
Also known as: TASR-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRYLRPPNT SLFVRNVADD TRSEDLRREF GRYGPIVDVY VPLDFYTRRP
60 70 80 90 100
RGFAYVQFED VRDAEDALHN LDRKWICGRQ IEIQFAQGDR KTPNQMKAKE
110 120 130 140 150
GRNVYSSSRY DDYDRYRRSR SRSYERRRSR SRSFDYNYRR SYSPRNSRPT
160 170 180 190 200
GRPRRSRSHS DNDRFKHRNR SFSRSKSNSR SRSKSQPKKE MKAKSRSRSA
210 220 230 240 250
SHTKTRGTSK TDSKTHYKSG SRYEKESRKK EPPRSKSQSR SQSRSRSKSR
260
SRSWTSPKSS GH
Length:262
Mass (Da):31,301
Last modified:November 1, 1998 - v1
Checksum:i205F95D36CBBFAB4
GO
Isoform 2 (identifier: O75494-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: Missing.

Show »
Length:261
Mass (Da):31,213
Checksum:iA07499B11D4C7570
GO
Isoform 3 (identifier: O75494-3) [UniParc]FASTAAdd to basket
Also known as: TASR-2, SRp38-2

The sequence of this isoform differs from the canonical sequence as follows:
     165-183: FKHRNRSFSRSKSNSRSRS → PNCSWNTQYSSAYYTSRKI
     184-262: Missing.

Show »
Length:183
Mass (Da):22,222
Checksum:i4AA87CAA9B51A131
GO
Isoform 4 (identifier: O75494-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     164-173: RFKHRNRSFS → SQVSKKKNER
     174-183: Missing.
     184-262: Missing.

Show »
Length:173
Mass (Da):21,000
Checksum:i4AB40FBF24846495
GO
Isoform 5 (identifier: O75494-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-165: NSRPTGRPRRSRSHSDNDRF → KPNCSWNTQYSSAYYTSRKI
     166-173: Missing.
     174-183: Missing.
     184-262: Missing.

Note: No experimental confirmation available.
Show »
Length:165
Mass (Da):20,117
Checksum:i6CB98688C53A9F69
GO
Isoform 6 (identifier: O75494-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-147: Missing.
     165-183: FKHRNRSFSRSKSNSRSRS → PNCSWNTQYSSAYYTSRKI
     184-262: Missing.

Note: No experimental confirmation available.
Show »
Length:182
Mass (Da):22,135
Checksum:iF99356E1D895987D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043697146 – 165NSRPT…DNDRF → KPNCSWNTQYSSAYYTSRKI in isoform 5. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_010421147Missing in isoform 2 and isoform 6. 1 Publication1
Alternative sequenceiVSP_010422164 – 173RFKHRNRSFS → SQVSKKKNER in isoform 4. Curated10
Alternative sequenceiVSP_010424165 – 183FKHRN…SRSRS → PNCSWNTQYSSAYYTSRKI in isoform 3 and isoform 6. 4 PublicationsAdd BLAST19
Alternative sequenceiVSP_043698166 – 173Missing in isoform 5. 1 Publication8
Alternative sequenceiVSP_010423174 – 183Missing in isoform 4 and isoform 5. 1 Publication10
Alternative sequenceiVSP_010425184 – 262Missing in isoform 3, isoform 4, isoform 5 and isoform 6. 4 PublicationsAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047448 mRNA. Translation: AAC70918.1.
AF067730 mRNA. Translation: AAC26727.1.
AF449427 mRNA. Translation: AAL57514.1.
AY150180 mRNA. Translation: AAN65380.1.
AY150181 mRNA. Translation: AAN65381.1.
AF419331 mRNA. Translation: AAL16665.1.
AF419332 Genomic DNA. Translation: AAL16666.1.
AY048592 Genomic DNA. Translation: AAL06098.1.
AY048592 Genomic DNA. Translation: AAL06099.1.
AK001286 mRNA. Translation: BAA91601.1.
AK001656 mRNA. Translation: BAG50956.1.
AK296175 mRNA. Translation: BAG58911.1.
AL590609 Genomic DNA. Translation: CAI14807.1.
AL590609 Genomic DNA. Translation: CAI14803.1.
AL590609 Genomic DNA. Translation: CAI14805.1.
AL590609 Genomic DNA. Translation: CAI14808.1.
BC001107 mRNA. Translation: AAH01107.1.
BC005039 mRNA. Translation: AAH05039.1.
BC010074 mRNA. Translation: AAH10074.1.
CCDSiCCDS30629.1. [O75494-3]
CCDS30630.1. [O75494-1]
CCDS53280.1. [O75494-5]
CCDS53281.1. [O75494-6]
CCDS53282.1. [O75494-2]
CCDS53283.1. [O75494-4]
RefSeqiNP_001177934.1. NM_001191005.2. [O75494-2]
NP_001177935.1. NM_001191006.2. [O75494-4]
NP_001177936.1. NM_001191007.2. [O75494-6]
NP_001177938.1. NM_001191009.2. [O75494-5]
NP_006616.1. NM_006625.5. [O75494-3]
NP_473357.1. NM_054016.3. [O75494-1]
UniGeneiHs.3530.

Genome annotation databases

EnsembliENST00000343255; ENSP00000344149; ENSG00000188529. [O75494-2]
ENST00000344989; ENSP00000342913; ENSG00000188529. [O75494-3]
ENST00000374452; ENSP00000363576; ENSG00000188529. [O75494-4]
ENST00000453840; ENSP00000388991; ENSG00000188529. [O75494-6]
ENST00000484146; ENSP00000419813; ENSG00000188529. [O75494-5]
ENST00000492112; ENSP00000420195; ENSG00000188529. [O75494-1]
GeneIDi10772.
KEGGihsa:10772.
UCSCiuc057dhz.1. human. [O75494-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047448 mRNA. Translation: AAC70918.1.
AF067730 mRNA. Translation: AAC26727.1.
AF449427 mRNA. Translation: AAL57514.1.
AY150180 mRNA. Translation: AAN65380.1.
AY150181 mRNA. Translation: AAN65381.1.
AF419331 mRNA. Translation: AAL16665.1.
AF419332 Genomic DNA. Translation: AAL16666.1.
AY048592 Genomic DNA. Translation: AAL06098.1.
AY048592 Genomic DNA. Translation: AAL06099.1.
AK001286 mRNA. Translation: BAA91601.1.
AK001656 mRNA. Translation: BAG50956.1.
AK296175 mRNA. Translation: BAG58911.1.
AL590609 Genomic DNA. Translation: CAI14807.1.
AL590609 Genomic DNA. Translation: CAI14803.1.
AL590609 Genomic DNA. Translation: CAI14805.1.
AL590609 Genomic DNA. Translation: CAI14808.1.
BC001107 mRNA. Translation: AAH01107.1.
BC005039 mRNA. Translation: AAH05039.1.
BC010074 mRNA. Translation: AAH10074.1.
CCDSiCCDS30629.1. [O75494-3]
CCDS30630.1. [O75494-1]
CCDS53280.1. [O75494-5]
CCDS53281.1. [O75494-6]
CCDS53282.1. [O75494-2]
CCDS53283.1. [O75494-4]
RefSeqiNP_001177934.1. NM_001191005.2. [O75494-2]
NP_001177935.1. NM_001191006.2. [O75494-4]
NP_001177936.1. NM_001191007.2. [O75494-6]
NP_001177938.1. NM_001191009.2. [O75494-5]
NP_006616.1. NM_006625.5. [O75494-3]
NP_473357.1. NM_054016.3. [O75494-1]
UniGeneiHs.3530.

3D structure databases

ProteinModelPortaliO75494.
SMRiO75494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115990. 79 interactors.
IntActiO75494. 28 interactors.
MINTiMINT-5002282.
STRINGi9606.ENSP00000420195.

PTM databases

iPTMnetiO75494.
PhosphoSitePlusiO75494.
SwissPalmiO75494.

Polymorphism and mutation databases

BioMutaiSRSF10.

Proteomic databases

EPDiO75494.
MaxQBiO75494.
PaxDbiO75494.
PeptideAtlasiO75494.
PRIDEiO75494.
TopDownProteomicsiO75494-1. [O75494-1]
O75494-2. [O75494-2]
O75494-3. [O75494-3]
O75494-4. [O75494-4]
O75494-5. [O75494-5]

Protocols and materials databases

DNASUi10772.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343255; ENSP00000344149; ENSG00000188529. [O75494-2]
ENST00000344989; ENSP00000342913; ENSG00000188529. [O75494-3]
ENST00000374452; ENSP00000363576; ENSG00000188529. [O75494-4]
ENST00000453840; ENSP00000388991; ENSG00000188529. [O75494-6]
ENST00000484146; ENSP00000419813; ENSG00000188529. [O75494-5]
ENST00000492112; ENSP00000420195; ENSG00000188529. [O75494-1]
GeneIDi10772.
KEGGihsa:10772.
UCSCiuc057dhz.1. human. [O75494-1]

Organism-specific databases

CTDi10772.
DisGeNETi10772.
GeneCardsiSRSF10.
HGNCiHGNC:16713. SRSF10.
HPAiHPA053805.
HPA053831.
MIMi605221. gene.
neXtProtiNX_O75494.
OpenTargetsiENSG00000188529.
PharmGKBiPA28427.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104403.
HOGENOMiHOG000276233.
HOVERGENiHBG107480.
InParanoidiO75494.
KOiK12900.
OMAiFAYVQYT.
OrthoDBiEOG091G13K0.
PhylomeDBiO75494.
TreeFamiTF351864.

Enzyme and pathway databases

BioCyciZFISH:G66-33709-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

GeneWikiiFUSIP1.
GenomeRNAii10772.
PROiO75494.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188529.
ExpressionAtlasiO75494. baseline and differential.
GenevisibleiO75494. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRS10_HUMAN
AccessioniPrimary (citable) accession number: O75494
Secondary accession number(s): A6NFM6
, A6NI42, A6NIU7, B4DJP9, O60572, Q5JRH9, Q5JRI0, Q5JRI2, Q5JRI3, Q5JRI4, Q96G09, Q96P17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.