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Protein

Glypican-4

Gene

GPC4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • anatomical structure morphogenesis Source: ProtInc
  • cell proliferation Source: ProtInc
  • glycosaminoglycan biosynthetic process Source: Reactome
  • glycosaminoglycan catabolic process Source: Reactome
  • glycosaminoglycan metabolic process Source: Reactome
  • retinoid metabolic process Source: Reactome
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000076716-MONOMER.
ReactomeiR-HSA-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
R-HSA-2022928. HS-GAG biosynthesis.
R-HSA-2024096. HS-GAG degradation.
R-HSA-3560783. Defective B4GALT7 causes EDS, progeroid type.
R-HSA-3560801. Defective B3GAT3 causes JDSSDHD.
R-HSA-3656237. Defective EXT2 causes exostoses 2.
R-HSA-3656253. Defective EXT1 causes exostoses 1, TRPS2 and CHDS.
R-HSA-4420332. Defective B3GALT6 causes EDSP2 and SEMDJL1.
R-HSA-975634. Retinoid metabolism and transport.
SIGNORiO75487.

Names & Taxonomyi

Protein namesi
Recommended name:
Glypican-4
Alternative name(s):
K-glypican
Cleaved into the following chain:
Gene namesi
Name:GPC4
ORF Names:UNQ474/PRO937
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:4452. GPC4.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB-SubCell
  • Golgi lumen Source: Reactome
  • integral component of plasma membrane Source: ProtInc
  • lysosomal lumen Source: Reactome
  • nucleus Source: UniProtKB
  • plasma membrane Source: Reactome
  • proteinaceous extracellular matrix Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi2239.
MalaCardsiGPC4.
OpenTargetsiENSG00000076716.
Orphaneti373. Simpson-Golabi-Behmel syndrome.
PharmGKBiPA28833.

Polymorphism and mutation databases

BioMutaiGPC4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001231519 – 529Glypican-4Add BLAST511
ChainiPRO_000033384719 – ?Secreted glypican-4
PropeptideiPRO_0000012316530 – 556Removed in mature formSequence analysisAdd BLAST27

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei357PhosphoserineCombined sources1
Glycosylationi494O-linked (Xyl...) (glycosaminoglycan)Sequence analysis1
Glycosylationi498O-linked (Xyl...) (glycosaminoglycan)Sequence analysis1
Glycosylationi500O-linked (Xyl...) (glycosaminoglycan)Sequence analysis1
Glycosylationi514N-linked (GlcNAc...)Sequence analysis1
Lipidationi529GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Heparan sulfate, Lipoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

EPDiO75487.
MaxQBiO75487.
PaxDbiO75487.
PeptideAtlasiO75487.
PRIDEiO75487.

PTM databases

iPTMnetiO75487.
PhosphoSitePlusiO75487.

Expressioni

Gene expression databases

BgeeiENSG00000076716.
CleanExiHS_GPC4.
GenevisibleiO75487. HS.

Organism-specific databases

HPAiHPA030836.

Interactioni

Protein-protein interaction databases

BioGridi108530. 19 interactors.
IntActiO75487. 11 interactors.
STRINGi9606.ENSP00000359864.

Structurei

3D structure databases

ProteinModelPortaliO75487.
SMRiO75487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glypican family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3821. Eukaryota.
ENOG410XST2. LUCA.
GeneTreeiENSGT00550000074430.
HOVERGENiHBG003464.
InParanoidiO75487.
KOiK08110.
OMAiDENYMRC.
OrthoDBiEOG091G06T6.
PhylomeDBiO75487.
TreeFamiTF105317.

Family and domain databases

InterProiIPR001863. Glypican.
IPR031180. Glypican-4.
IPR019803. Glypican_CS.
[Graphical view]
PANTHERiPTHR10822. PTHR10822. 1 hit.
PTHR10822:SF25. PTHR10822:SF25. 1 hit.
PfamiPF01153. Glypican. 1 hit.
[Graphical view]
PROSITEiPS01207. GLYPICAN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75487-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARFGLPALL CTLAVLSAAL LAAELKSKSC SEVRRLYVSK GFNKNDAPLH
60 70 80 90 100
EINGDHLKIC PQGSTCCSQE MEEKYSLQSK DDFKSVVSEQ CNHLQAVFAS
110 120 130 140 150
RYKKFDEFFK ELLENAEKSL NDMFVKTYGH LYMQNSELFK DLFVELKRYY
160 170 180 190 200
VVGNVNLEEM LNDFWARLLE RMFRLVNSQY HFTDEYLECV SKYTEQLKPF
210 220 230 240 250
GDVPRKLKLQ VTRAFVAART FAQGLAVAGD VVSKVSVVNP TAQCTHALLK
260 270 280 290 300
MIYCSHCRGL VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE
310 320 330 340 350
RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG
360 370 380 390 400
RISRSISESA FSARFRPHHP EERPTTAAGT SLDRLVTDVK EKLKQAKKFW
410 420 430 440 450
SSLPSNVCND ERMAAGNGNE DDCWNGKGKS RYLFAVTGNG LANQGNNPEV
460 470 480 490 500
QVDTSKPDIL ILRQIMALRV MTSKMKNAYN GNDVDFFDIS DESSGEGSGS
510 520 530 540 550
GCEYQQCPSE FDYNATDHAG KSANEKADSA GVRPGAQAYL LTVFCILFLV

MQREWR
Length:556
Mass (Da):62,412
Last modified:May 16, 2003 - v4
Checksum:i827E07FAA0BD8188
GO
Isoform 2 (identifier: O75487-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.

Note: No experimental confirmation available.
Show »
Length:486
Mass (Da):54,910
Checksum:iB83D796B5B026A96
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_016191391E → D.4 PublicationsCorresponds to variant rs1129980dbSNPEnsembl.1
Natural variantiVAR_016192442A → V.3 PublicationsCorresponds to variant rs1048369dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0565701 – 70Missing in isoform 2. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030186 mRNA. Translation: AAC69991.1.
AF064826 mRNA. Translation: AAC31899.1.
AY358507 mRNA. Translation: AAQ88871.1.
AY052833 mRNA. Translation: AAL11018.1.
AK304207 mRNA. Translation: BAG65082.1.
AK312605 mRNA. Translation: BAG35494.1.
AL034400, AL109623 Genomic DNA. Translation: CAI42076.1.
AL109623, AL034400 Genomic DNA. Translation: CAB86664.2.
CH471107 Genomic DNA. Translation: EAX11772.1.
BC017166 mRNA. Translation: AAH17166.1.
CCDSiCCDS14637.1. [O75487-1]
RefSeqiNP_001439.2. NM_001448.2. [O75487-1]
UniGeneiHs.58367.

Genome annotation databases

EnsembliENST00000370828; ENSP00000359864; ENSG00000076716. [O75487-1]
GeneIDi2239.
KEGGihsa:2239.
UCSCiuc004exc.2. human. [O75487-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030186 mRNA. Translation: AAC69991.1.
AF064826 mRNA. Translation: AAC31899.1.
AY358507 mRNA. Translation: AAQ88871.1.
AY052833 mRNA. Translation: AAL11018.1.
AK304207 mRNA. Translation: BAG65082.1.
AK312605 mRNA. Translation: BAG35494.1.
AL034400, AL109623 Genomic DNA. Translation: CAI42076.1.
AL109623, AL034400 Genomic DNA. Translation: CAB86664.2.
CH471107 Genomic DNA. Translation: EAX11772.1.
BC017166 mRNA. Translation: AAH17166.1.
CCDSiCCDS14637.1. [O75487-1]
RefSeqiNP_001439.2. NM_001448.2. [O75487-1]
UniGeneiHs.58367.

3D structure databases

ProteinModelPortaliO75487.
SMRiO75487.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108530. 19 interactors.
IntActiO75487. 11 interactors.
STRINGi9606.ENSP00000359864.

PTM databases

iPTMnetiO75487.
PhosphoSitePlusiO75487.

Polymorphism and mutation databases

BioMutaiGPC4.

Proteomic databases

EPDiO75487.
MaxQBiO75487.
PaxDbiO75487.
PeptideAtlasiO75487.
PRIDEiO75487.

Protocols and materials databases

DNASUi2239.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370828; ENSP00000359864; ENSG00000076716. [O75487-1]
GeneIDi2239.
KEGGihsa:2239.
UCSCiuc004exc.2. human. [O75487-1]

Organism-specific databases

CTDi2239.
DisGeNETi2239.
GeneCardsiGPC4.
GeneReviewsiGPC4.
HGNCiHGNC:4452. GPC4.
HPAiHPA030836.
MalaCardsiGPC4.
MIMi300168. gene.
neXtProtiNX_O75487.
OpenTargetsiENSG00000076716.
Orphaneti373. Simpson-Golabi-Behmel syndrome.
PharmGKBiPA28833.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3821. Eukaryota.
ENOG410XST2. LUCA.
GeneTreeiENSGT00550000074430.
HOVERGENiHBG003464.
InParanoidiO75487.
KOiK08110.
OMAiDENYMRC.
OrthoDBiEOG091G06T6.
PhylomeDBiO75487.
TreeFamiTF105317.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000076716-MONOMER.
ReactomeiR-HSA-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
R-HSA-2022928. HS-GAG biosynthesis.
R-HSA-2024096. HS-GAG degradation.
R-HSA-3560783. Defective B4GALT7 causes EDS, progeroid type.
R-HSA-3560801. Defective B3GAT3 causes JDSSDHD.
R-HSA-3656237. Defective EXT2 causes exostoses 2.
R-HSA-3656253. Defective EXT1 causes exostoses 1, TRPS2 and CHDS.
R-HSA-4420332. Defective B3GALT6 causes EDSP2 and SEMDJL1.
R-HSA-975634. Retinoid metabolism and transport.
SIGNORiO75487.

Miscellaneous databases

ChiTaRSiGPC4. human.
GeneWikiiGlypican_4.
GenomeRNAii2239.
PROiO75487.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000076716.
CleanExiHS_GPC4.
GenevisibleiO75487. HS.

Family and domain databases

InterProiIPR001863. Glypican.
IPR031180. Glypican-4.
IPR019803. Glypican_CS.
[Graphical view]
PANTHERiPTHR10822. PTHR10822. 1 hit.
PTHR10822:SF25. PTHR10822:SF25. 1 hit.
PfamiPF01153. Glypican. 1 hit.
[Graphical view]
PROSITEiPS01207. GLYPICAN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPC4_HUMAN
AccessioniPrimary (citable) accession number: O75487
Secondary accession number(s): B2R6J7
, B4E2C0, Q6ZMA6, Q96L43, Q9NU08, Q9UJN1, Q9UPD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 16, 2003
Last modified: November 30, 2016
This is version 151 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.