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Protein

Transcription initiation protein SPT3 homolog

Gene

SUPT3H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcriptional activator.1 Publication

GO - Molecular functioni

  • DNA binding Source: GO_Central
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

  • histone deubiquitination Source: UniProtKB
  • histone H3 acetylation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
  • transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172371-MONOMER.
ReactomeiR-HSA-3214847. HATs acetylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation protein SPT3 homolog
Alternative name(s):
SPT3-like protein
Gene namesi
Name:SUPT3H
Synonyms:SPT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:11466. SUPT3H.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • SAGA complex Source: GO_Central
  • STAGA complex Source: UniProtKB
  • transcription factor TFTC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8464.
OpenTargetsiENSG00000196284.
PharmGKBiPA36252.

Polymorphism and mutation databases

BioMutaiSUPT3H.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000723131 – 399Transcription initiation protein SPT3 homologAdd BLAST399

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Isoform 2 (identifier: O75486-2)
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiO75486.
MaxQBiO75486.
PaxDbiO75486.
PeptideAtlasiO75486.
PRIDEiO75486.

PTM databases

iPTMnetiO75486.
PhosphoSitePlusiO75486.

Expressioni

Tissue specificityi

Expressed in all tissues tested including pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Gene expression databases

BgeeiENSG00000196284.
CleanExiHS_SUPT3H.
ExpressionAtlasiO75486. baseline and differential.
GenevisibleiO75486. HS.

Organism-specific databases

HPAiHPA024371.

Interactioni

Subunit structurei

Component of the PCAF complex, at least composed of TADA2L/ADA2, SUPT3H, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. Associates with TAFII31 and GCN5L2. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22.4 Publications

Protein-protein interaction databases

BioGridi114042. 35 interactors.
DIPiDIP-28148N.
IntActiO75486. 6 interactors.
STRINGi9606.ENSP00000360515.

Structurei

3D structure databases

ProteinModelPortaliO75486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SPT3 family.Curated

Phylogenomic databases

eggNOGiKOG3902. Eukaryota.
ENOG410Z86C. LUCA.
GeneTreeiENSGT00390000010738.
HOGENOMiHOG000294119.
HOVERGENiHBG058560.
InParanoidiO75486.
KOiK11313.
OMAiHPVKIAR.
OrthoDBiEOG091G0NTZ.
PhylomeDBiO75486.
TreeFamiTF323574.

Family and domain databases

CDDicd07978. TAF13. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003195. TFIID_TAF13.
[Graphical view]
PANTHERiPTHR11380. PTHR11380. 1 hit.
PfamiPF02269. TFIID-18kDa. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75486-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYSPRGSQGR GTAEATANSP SPPIAPSHSR VTFSLSTLHT LSPPPRPFPS
60 70 80 90 100
VSRAAAQKPH HLHPHILLAG SAAVPPRVLK AEMNNTAASP MSTATSSSGR
110 120 130 140 150
STGKSISFAT ELQSMMYSLG DARRPLHETA VLVEDVVHTQ LINLLQQAAE
160 170 180 190 200
VSQLRGARVI TPEDLLFLMR KDKKKLRRLL KYMFIRDYKS KIVKGIDEDD
210 220 230 240 250
LLEDKLSGSN NANKRQKIAQ DFLNSIDQTG ELLAMFEDDE IDEVKQERME
260 270 280 290 300
RAERQTRIMD SAQYAEFCES RQLSFSKKAS KFRDWLDCSS MEIKPNVVAM
310 320 330 340 350
EILAYLAYET VAQLVDLALL VRQDMVTKAG DPFSHAISAT FIQYHNSAES
360 370 380 390
TAACGVEAHS DAIQPCHIRE AIRRYSHRIG PLSPFTNAYR RNGMAFLAC
Length:399
Mass (Da):44,362
Last modified:May 15, 2002 - v2
Checksum:i06CD35E28A301F35
GO
Isoform 2 (identifier: O75486-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Show »
Length:317
Mass (Da):35,793
Checksum:iAF339954A57DB5A6
GO
Isoform 3 (identifier: O75486-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: MYSPRGSQGR...SFATELQSMM → MVVFGTTFFN...ILPLLNDSGR

Show »
Length:328
Mass (Da):37,427
Checksum:i3E2B1193CD0C5393
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124 – 128RPLHE → SLFMR in AAC70014 (PubMed:9787080).Curated5
Sequence conflicti255 – 257QTR → HNS in AAC70014 (PubMed:9787080).Curated3
Sequence conflicti306L → S in BAG36602 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057000324D → A.Corresponds to variant rs16872923dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0358781 – 116MYSPR…LQSMM → MVVFGTTFFNFDSFIGKTDS IEIIGKSVFPYCYHNILPLL NDSGR in isoform 3. 1 PublicationAdd BLAST116
Alternative sequenceiVSP_0039751 – 82Missing in isoform 2. 3 PublicationsAdd BLAST82

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064804 mRNA. Translation: AAC70014.1.
AF069734 mRNA. Translation: AAC39904.1.
AF073930 mRNA. Translation: AAC36098.1.
AK313874 mRNA. Translation: BAG36602.1.
AL096865
, AL138880, AL161905, AL360272 Genomic DNA. Translation: CAI19927.1.
AL138880
, AL096865, AL161905, AL360272 Genomic DNA. Translation: CAI14627.1.
AL161905
, AL096865, AL138880, AL360272 Genomic DNA. Translation: CAI15666.1.
AL360272
, AL096865, AL138880, AL161905 Genomic DNA. Translation: CAH70848.1.
AL360272
, AL138880, AL161905, AL499610 Genomic DNA. Translation: CAH70846.1.
AL499610
, AL138880, AL161905, AL360272 Genomic DNA. Translation: CAH73344.1.
AL138880
, AL161905, AL360272, AL499610 Genomic DNA. Translation: CAI14625.1.
AL161905
, AL138880, AL360272, AL499610 Genomic DNA. Translation: CAI15664.1.
CH471081 Genomic DNA. Translation: EAX04274.1.
CH471081 Genomic DNA. Translation: EAX04275.1.
BC050384 mRNA. Translation: AAH50384.1.
CCDSiCCDS34465.1. [O75486-2]
CCDS34466.1. [O75486-3]
RefSeqiNP_003590.1. NM_003599.3. [O75486-2]
NP_852001.1. NM_181356.2. [O75486-3]
XP_016866860.1. XM_017011371.1. [O75486-2]
XP_016866861.1. XM_017011372.1. [O75486-2]
UniGeneiHs.368325.

Genome annotation databases

EnsembliENST00000371459; ENSP00000360514; ENSG00000196284. [O75486-2]
ENST00000371460; ENSP00000360515; ENSG00000196284. [O75486-3]
ENST00000475057; ENSP00000436411; ENSG00000196284. [O75486-2]
GeneIDi8464.
KEGGihsa:8464.
UCSCiuc003oxn.3. human. [O75486-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064804 mRNA. Translation: AAC70014.1.
AF069734 mRNA. Translation: AAC39904.1.
AF073930 mRNA. Translation: AAC36098.1.
AK313874 mRNA. Translation: BAG36602.1.
AL096865
, AL138880, AL161905, AL360272 Genomic DNA. Translation: CAI19927.1.
AL138880
, AL096865, AL161905, AL360272 Genomic DNA. Translation: CAI14627.1.
AL161905
, AL096865, AL138880, AL360272 Genomic DNA. Translation: CAI15666.1.
AL360272
, AL096865, AL138880, AL161905 Genomic DNA. Translation: CAH70848.1.
AL360272
, AL138880, AL161905, AL499610 Genomic DNA. Translation: CAH70846.1.
AL499610
, AL138880, AL161905, AL360272 Genomic DNA. Translation: CAH73344.1.
AL138880
, AL161905, AL360272, AL499610 Genomic DNA. Translation: CAI14625.1.
AL161905
, AL138880, AL360272, AL499610 Genomic DNA. Translation: CAI15664.1.
CH471081 Genomic DNA. Translation: EAX04274.1.
CH471081 Genomic DNA. Translation: EAX04275.1.
BC050384 mRNA. Translation: AAH50384.1.
CCDSiCCDS34465.1. [O75486-2]
CCDS34466.1. [O75486-3]
RefSeqiNP_003590.1. NM_003599.3. [O75486-2]
NP_852001.1. NM_181356.2. [O75486-3]
XP_016866860.1. XM_017011371.1. [O75486-2]
XP_016866861.1. XM_017011372.1. [O75486-2]
UniGeneiHs.368325.

3D structure databases

ProteinModelPortaliO75486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114042. 35 interactors.
DIPiDIP-28148N.
IntActiO75486. 6 interactors.
STRINGi9606.ENSP00000360515.

PTM databases

iPTMnetiO75486.
PhosphoSitePlusiO75486.

Polymorphism and mutation databases

BioMutaiSUPT3H.

Proteomic databases

EPDiO75486.
MaxQBiO75486.
PaxDbiO75486.
PeptideAtlasiO75486.
PRIDEiO75486.

Protocols and materials databases

DNASUi8464.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371459; ENSP00000360514; ENSG00000196284. [O75486-2]
ENST00000371460; ENSP00000360515; ENSG00000196284. [O75486-3]
ENST00000475057; ENSP00000436411; ENSG00000196284. [O75486-2]
GeneIDi8464.
KEGGihsa:8464.
UCSCiuc003oxn.3. human. [O75486-1]

Organism-specific databases

CTDi8464.
DisGeNETi8464.
GeneCardsiSUPT3H.
HGNCiHGNC:11466. SUPT3H.
HPAiHPA024371.
MIMi602947. gene.
neXtProtiNX_O75486.
OpenTargetsiENSG00000196284.
PharmGKBiPA36252.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3902. Eukaryota.
ENOG410Z86C. LUCA.
GeneTreeiENSGT00390000010738.
HOGENOMiHOG000294119.
HOVERGENiHBG058560.
InParanoidiO75486.
KOiK11313.
OMAiHPVKIAR.
OrthoDBiEOG091G0NTZ.
PhylomeDBiO75486.
TreeFamiTF323574.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172371-MONOMER.
ReactomeiR-HSA-3214847. HATs acetylate histones.

Miscellaneous databases

ChiTaRSiSUPT3H. human.
GeneWikiiTranscription_initiation_protein_SPT3_homolog.
GenomeRNAii8464.
PROiO75486.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196284.
CleanExiHS_SUPT3H.
ExpressionAtlasiO75486. baseline and differential.
GenevisibleiO75486. HS.

Family and domain databases

CDDicd07978. TAF13. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR003195. TFIID_TAF13.
[Graphical view]
PANTHERiPTHR11380. PTHR11380. 1 hit.
PfamiPF02269. TFIID-18kDa. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSUPT3_HUMAN
AccessioniPrimary (citable) accession number: O75486
Secondary accession number(s): A6NKG9
, B2R9Q5, O76066, Q5TAV9, Q86VN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: May 15, 2002
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.