O75478 (TAD2A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional adapter 2-alpha

Alternative name(s):
Transcriptional adapter 2-like
Short name=ADA2-like protein

Gene names

Name:	TADA2A
Synonyms:	TADA2L
ORF Names:	KL04P

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a distant site By similarity. Binds double-stranded DNA. Binds dinucleosomes, probably at the linker region between neighboring nucleosomes. Plays a role in chromatin remodeling. Ref.9
Subunit structure	Interacts with GCN5 and NR3C1. Associated with the P/CAF protein in the PCAF complex. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Ref.7
Subcellular location	Nucleus. Chromosome.
Tissue specificity	Expressed in all tissues, but most abundantly in testis.
Sequence similarities	Contains 1 SANT domain. Contains 1 SWIRM domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histone H3 acetylation Inferred from direct assay Ref.6. Source: UniProtKB transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc
Cellular component	PCAF complex Inferred from direct assay Ref.6. Source: UniProtKB chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc transcription cofactor activity Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75478-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75478-2)

The sequence of this isoform differs from the canonical sequence as follows:
     275-305: LEFELRREIKRLQEYRTAGITNFCSARTYDH → CRWFLSLEQYLCVYIYINRRDNGVFYVKFYK
     306-443: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 443

443

Transcriptional adapter 2-alpha

PRO_0000197083

Regions

Domain

70 – 122

53

SANT

Domain

356 – 443

88

SWIRM

DNA binding

426 – 435

10

By similarity

Compositional bias

17 – 45

29

Cys-rich

Natural variations

Alternative sequence

275 – 305

31

LEFEL…RTYDH → CRWFLSLEQYLCVYIYINRR DNGVFYVKFYK in isoform 2.

VSP_040347

Alternative sequence

306 – 443

138

Missing in isoform 2.

VSP_040348

Natural variant

6

1

P → S. Ref.1 Ref.2 Ref.3 Ref.5
Corresponds to variant rs7211875 [ dbSNP | Ensembl ].

VAR_047466

Natural variant

115

1

M → V. Ref.3
Corresponds to variant rs1054865 [ dbSNP | Ensembl ].

VAR_047467

Natural variant

351

1

I → M.
Corresponds to variant rs2522969 [ dbSNP | Ensembl ].

VAR_047468

Experimental info

Sequence conflict

117

1

H → Y in AAH01172. Ref.5

Sequence conflict

153

1

P → L in AAC39902. Ref.6

Sequence conflict

185

1

W → R in AAC26659. Ref.2

Sequence conflict

304

1

D → N in AAC26659. Ref.2

Sequence conflict

342

1

D → G in AAC26659. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

443

Helix Strand Turn

Details...

Beta strand

73 – 75

3

Helix

77 – 89

13

Helix

95 – 102

8

Helix

107 – 117

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: A4E1D87B1B3F6BA5	FASTA	443	51,506
	10 20 30 40 50 60 MDRLGPFSND PSDKPPCRGC SSYLMEPYIK CAECGPPPFF LCLQCFTRGF EYKKHQSDHT 70 80 90 100 110 120 YEIMTSDFPV LDPSWTAQEE MALLEAVMDC GFGNWQDVAN QMCTKTKEEC EKHYMKHFIN 130 140 150 160 170 180 NPLFASTLLN LKQAEEAKTA DTAIPFHSTD DPPRPTFDSL LSRDMAGYMP ARADFIEEFD 190 200 210 220 230 240 NYAEWDLRDI DFVEDDSDIL HALKMAVVDI YHSRLKERQR RKKIIRDHGL INLRKFQLME 250 260 270 280 290 300 RRYPKEVQDL YETMRRFARI VGPVEHDKFI ESHALEFELR REIKRLQEYR TAGITNFCSA 310 320 330 340 350 360 RTYDHLKKTR EEERLKRTML SEVLQYIQDS SACQQWLRRQ ADIDSGLSPS IPMASNSGRR 370 380 390 400 410 420 SAPPLNLTGL PGTEKLNEKE KELCQMVRLV PGAYLEYKSA LLNECNKQGG LRLAQARALI 430 440 KIDVNKTRKI YDFLIREGYI TKG
	Isoform 2 [UniParc]. Checksum: 51386043D3BB294C	FASTA	305	36,054
	10 20 30 40 50 60 MDRLGPFSND PSDKPPCRGC SSYLMEPYIK CAECGPPPFF LCLQCFTRGF EYKKHQSDHT 70 80 90 100 110 120 YEIMTSDFPV LDPSWTAQEE MALLEAVMDC GFGNWQDVAN QMCTKTKEEC EKHYMKHFIN 130 140 150 160 170 180 NPLFASTLLN LKQAEEAKTA DTAIPFHSTD DPPRPTFDSL LSRDMAGYMP ARADFIEEFD 190 200 210 220 230 240 NYAEWDLRDI DFVEDDSDIL HALKMAVVDI YHSRLKERQR RKKIIRDHGL INLRKFQLME 250 260 270 280 290 300 RRYPKEVQDL YETMRRFARI VGPVEHDKFI ESHACRWFLS LEQYLCVYIY INRRDNGVFY VKFYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of human proteins functionally conserved with the yeast putative adaptors ADA2 and GCN5." Candau R., Moore P.A., Wang L., Barlev N., Ying C.Y., Rosen C.A., Berger S.L. Mol. Cell. Biol. 16:593-602(1996) [PubMed: 8552087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, VARIANT SER-6. Tissue: Testis.
[2]	"A novel gene from human dendritic cell." Huang X., Li N., Zhao Z., Zhu X., Cao X. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-6. Tissue: Dendritic cell.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-6 AND VAL-115.
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-6. Tissue: Eye and Muscle.
[6]	"Histone-like TAFs within the PCAF histone acetylase complex." Ogryzko V.V., Kotani T., Zhang X., Schiltz R.L., Howard T., Yang X.-J., Howard B.H., Qin J., Nakatani Y. Cell 94:35-44(1998) [PubMed: 9674425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 81-443, MASS SPECTROMETRY.
[7]	"Role of the Ada adaptor complex in gene activation by the glucocorticoid receptor." Henriksson A., Almloef T., Ford J., McEwan I.J., Gustafsson J.-A., Wright A.P.H. Mol. Cell. Biol. 17:3065-3073(1997) [PubMed: 9154805] [Abstract] Cited for: INTERACTION WITH NR3C1.
[8]	"The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily." Vassilev A., Yamauchi J., Kotani T., Prives C., Avantaggiati M.L., Qin J., Nakatani Y. Mol. Cell 2:869-875(1998) [PubMed: 9885574] [Abstract] Cited for: IDENTIFICATION IN THE PCAF COMPLEX WITH TRRAP; TADA3L; TAF5L; SUPT3H; TAF6L; TAF10; TAF12 AND TAF9.
[9]	"The double-histone-acetyltransferase complex ATAC is essential for mammalian development." Guelman S., Kozuka K., Mao Y., Pham V., Solloway M.J., Wang J., Wu J., Lill J.R., Zha J. Mol. Cell. Biol. 29:1176-1188(2009) [PubMed: 19103755] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN ATAC COMPLEX.
[10]	"Solution structure of the Myb-like DNA binding domain of human transcriptional adaptor 2-like, isoform B." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 72-118.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF064094 mRNA. Translation: AAC26659.1.
AK022767 mRNA. Translation: BAG51111.1.
AC004099 Genomic DNA. No translation available.
AC068400 Genomic DNA. No translation available.
AC068447 Genomic DNA. No translation available.
BC001172 mRNA. Translation: AAH01172.1.
BC011753 mRNA. Translation: AAH11753.1.
AF069732 mRNA. Translation: AAC39902.1.

IPI

IPI00289581.
IPI00306347.

RefSeq

NP_001159577.1. NM_001166105.1.
NP_001479.3. NM_001488.3.
NP_597683.2. NM_133439.2.

UniGene

Hs.500066.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X41	NMR	-	A	72-116	[»]

ProteinModelPortal

O75478.

SMR

O75478. Positions 13-65, 72-118, 354-443.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-28151N.

IntAct

O75478. 7 interactions.

MINT

MINT-1447145.

STRING

O75478.

Proteomic databases

PRIDE

O75478.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000225396; ENSP00000225396; ENSG00000108264.
ENST00000394395; ENSP00000377918; ENSG00000108264.
ENST00000417170; ENSP00000406699; ENSG00000108264.

GeneID

6871.

KEGG

hsa:6871.

UCSC

uc002hnt.1. human.

Organism-specific databases

CTD

6871.

GeneCards

GC17P035767.

H-InvDB

HIX0013744.

HGNC

HGNC:11531. TADA2A.

MIM

602276. gene.

neXtProt

NX_O75478.

PharmGKB

PA165433076.
PA36306.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08219.

GeneTree

ENSGT00530000063657.

HOGENOM

HBG714058.

HOVERGEN

HBG057413.

InParanoid

O75478.

OMA

DPFDKPP.

OrthoDB

EOG4BK53W.

PhylomeDB

O75478.

Gene expression databases

ArrayExpress

O75478.

Bgee

O75478.

CleanEx

HS_TADA2L.

Genevestigator

O75478.

GermOnline

ENSG00000108264. Homo sapiens.

Family and domain databases

InterPro

IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR014778. Myb_DNA-bd.
IPR001005. SANT_DNA-bd.
IPR017884. SANT_eukarya.
IPR007526. SWIRM.
IPR016827. Transcriptional_adaptor_2.
IPR000433. Znf_ZZ.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

KO

K11314.

Pfam

PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
[Graphical view]

PIRSF

PIRSF025024. Transcriptional_adaptor_2. 1 hit.

SMART

SM00717. SANT. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 2 hits.

PROSITE

PS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

26821.

SOURCE

Search...

Entry information

Entry name

TAD2A_HUMAN

Accession

Primary (citable) accession number: O75478
Secondary accession number(s): A8MVD0 , B3KMU9, Q9BVJ0, Q9UCW2, Q9UP49

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 11, 2011
Last modified:	January 25, 2012
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.