Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O75452

- RDH16_HUMAN

UniProt

O75452 - RDH16_HUMAN

Protein

Retinol dehydrogenase 16

Gene

RDH16

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (23 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Oxidoreductase with a preference for NAD. Oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. Has higher activity towards CRBP-bound retinol than with free retinol. Oxidizes 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction.3 Publications

    Enzyme regulationi

    Inhibited by citral, perillyl alcohol, geraniol, farnesol and geranyl geraniol.1 Publication

    Kineticsi

    1. KM=0.14 µM for androsterone1 Publication
    2. KM=0.22 µM for androstanediol1 Publication
    3. KM=0.80 µM for dihydrotestosterone1 Publication
    4. KM=0.13 µM for NAD1 Publication
    5. KM=190 µM for NADP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei164 – 1641SubstrateBy similarity
    Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 5725NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. electron carrier activity Source: UniProtKB
    2. retinol dehydrogenase activity Source: ProtInc

    GO - Biological processi

    1. lipid metabolic process Source: ProtInc

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:ENSG00000139547-MONOMER.
    ReactomeiREACT_160156. The canonical retinoid cycle in rods (twilight vision).
    SABIO-RKO75452.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Retinol dehydrogenase 16 (EC:1.1.-.-)
    Alternative name(s):
    Microsomal NAD(+)-dependent retinol dehydrogenase 4
    Short name:
    RoDH-4
    Sterol/retinol dehydrogenase
    Gene namesi
    Name:RDH16
    Synonyms:RODH4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:29674. RDH16.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intracellular membrane-bounded organelle Source: ProtInc

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671089.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Retinol dehydrogenase 16PRO_0000307693Add
    BLAST

    Post-translational modificationi

    Not N-glycosylated.

    Proteomic databases

    PaxDbiO75452.
    PRIDEiO75452.

    PTM databases

    PhosphoSiteiO75452.

    Expressioni

    Tissue specificityi

    Highly expressed in adult liver (at protein level). Detected in endometrium, liver and foreskin. Detected in the spineous layers of adult skin, and at lower levels in basal and granular skin layers. Detected in fetal liver and lung.3 Publications

    Inductioni

    Transiently up-regulated by retinoic acid.1 Publication

    Gene expression databases

    BgeeiO75452.
    CleanExiHS_RDH16.
    GenevestigatoriO75452.

    Organism-specific databases

    HPAiHPA038518.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000381206.

    Structurei

    3D structure databases

    ProteinModelPortaliO75452.
    SMRiO75452. Positions 32-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 288288CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini310 – 3178LumenalSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei289 – 30921Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1028.
    HOVERGENiHBG005482.
    InParanoidiO75452.
    KOiK11154.
    OMAiVKECVRD.
    OrthoDBiEOG7FXZZX.
    PhylomeDBiO75452.
    TreeFamiTF325617.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O75452-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLYLAVFVG LYYLLHWYRE RQVLSHLRDK YVFITGCDSG FGKLLARQLD    50
    ARGLRVLAAC LTEKGAEQLR GQTSDRLETV TLDVTKTESV AAAAQWVKEC 100
    VRDKGLWGLV NNAGISLPTA PNELLTKQDF VTILDVNLLG VIDVTLSLLP 150
    LVRRARGRVV NVSSVMGRVS LFGGGYCISK YGVEAFSDSL RRELSYFGVK 200
    VAMIEPGYFK TAVTSKERFL KSFLEIWDRS SPEVKEAYGE KFVADYKKSA 250
    EQMEQKCTQD LSLVTNCMEH ALIACHPRTR YSAGWDAKLL YLPMSYMPTF 300
    LVDAIMYWVS PSPAKAL 317
    Length:317
    Mass (Da):35,673
    Last modified:October 23, 2007 - v2
    Checksum:iF9208C22B0515CFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti130 – 1312FV → LL in AAC72923. (PubMed:10329026)Curated
    Sequence conflicti147 – 1471S → N in AAC72923. (PubMed:10329026)Curated
    Sequence conflicti154 – 1541R → K in AAC39922. (PubMed:9677409)Curated
    Sequence conflicti163 – 1631S → F in AAC72923. (PubMed:10329026)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057034 mRNA. Translation: AAC39922.1.
    AF086735 mRNA. Translation: AAC72923.1.
    CCDSiCCDS41797.1.
    RefSeqiNP_003699.3. NM_003708.3.
    UniGeneiHs.134958.

    Genome annotation databases

    EnsembliENST00000398138; ENSP00000381206; ENSG00000139547.
    GeneIDi8608.
    KEGGihsa:8608.
    UCSCiuc001smi.4. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057034 mRNA. Translation: AAC39922.1 .
    AF086735 mRNA. Translation: AAC72923.1 .
    CCDSi CCDS41797.1.
    RefSeqi NP_003699.3. NM_003708.3.
    UniGenei Hs.134958.

    3D structure databases

    ProteinModelPortali O75452.
    SMRi O75452. Positions 32-281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000381206.

    PTM databases

    PhosphoSitei O75452.

    Proteomic databases

    PaxDbi O75452.
    PRIDEi O75452.

    Protocols and materials databases

    DNASUi 8608.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000398138 ; ENSP00000381206 ; ENSG00000139547 .
    GeneIDi 8608.
    KEGGi hsa:8608.
    UCSCi uc001smi.4. human.

    Organism-specific databases

    CTDi 8608.
    GeneCardsi GC12M057346.
    H-InvDB HIX0036688.
    HGNCi HGNC:29674. RDH16.
    HPAi HPA038518.
    neXtProti NX_O75452.
    PharmGKBi PA142671089.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1028.
    HOVERGENi HBG005482.
    InParanoidi O75452.
    KOi K11154.
    OMAi VKECVRD.
    OrthoDBi EOG7FXZZX.
    PhylomeDBi O75452.
    TreeFami TF325617.

    Enzyme and pathway databases

    BioCyci MetaCyc:ENSG00000139547-MONOMER.
    Reactomei REACT_160156. The canonical retinoid cycle in rods (twilight vision).
    SABIO-RK O75452.

    Miscellaneous databases

    GenomeRNAii 8608.
    NextBioi 32255.
    PROi O75452.

    Gene expression databases

    Bgeei O75452.
    CleanExi HS_RDH16.
    Genevestigatori O75452.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and characterization of a new human microsomal NAD+-dependent dehydrogenase that oxidizes all-trans-retinol and 3alpha-hydroxysteroids."
      Gough W.H., VanOoteghem S., Sint T., Kedishvili N.Y.
      J. Biol. Chem. 273:19778-19785(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "Cloning and characterization of retinol dehydrogenase transcripts expressed in human epidermal keratinocytes."
      Jurukovski V., Markova N.G., Karaman-Jurukovska N., Randolph R.K., Su J., Napoli J.L., Simon M.
      Mol. Genet. Metab. 67:62-73(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
      Tissue: Keratinocyte.
    3. "Expression of a retinol dehydrogenase (hRoDH-4), a member of the retinol/steroid dehydrogenase family implicated in retinoic acid biosynthesis, in normal and neoplastic endometria."
      Cain J.M., Zaino R., Shearer D., Bennett R.A., Olt G., Weisz J.
      Am. J. Obstet. Gynecol. 186:675-683(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4."
      Lapshina E.A., Belyaeva O.V., Chumakova O.V., Kedishvili N.Y.
      Biochemistry 42:776-784(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, LACK OF GLYCOSYLATION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiRDH16_HUMAN
    AccessioniPrimary (citable) accession number: O75452
    Secondary accession number(s): Q9UNV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3