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O75452

- RDH16_HUMAN

UniProt

O75452 - RDH16_HUMAN

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Protein

Retinol dehydrogenase 16

Gene

RDH16

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Oxidoreductase with a preference for NAD. Oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. Has higher activity towards CRBP-bound retinol than with free retinol. Oxidizes 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction.3 Publications

Enzyme regulationi

Inhibited by citral, perillyl alcohol, geraniol, farnesol and geranyl geraniol.1 Publication

Kineticsi

  1. KM=0.14 µM for androsterone1 Publication
  2. KM=0.22 µM for androstanediol1 Publication
  3. KM=0.80 µM for dihydrotestosterone1 Publication
  4. KM=0.13 µM for NAD1 Publication
  5. KM=190 µM for NADP1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641SubstrateBy similarity
Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 5725NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. electron carrier activity Source: UniProtKB
  2. retinol dehydrogenase activity Source: ProtInc

GO - Biological processi

  1. lipid metabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000139547-MONOMER.
ReactomeiREACT_160156. The canonical retinoid cycle in rods (twilight vision).
SABIO-RKO75452.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinol dehydrogenase 16 (EC:1.1.-.-)
Alternative name(s):
Microsomal NAD(+)-dependent retinol dehydrogenase 4
Short name:
RoDH-4
Sterol/retinol dehydrogenase
Gene namesi
Name:RDH16
Synonyms:RODH4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:29674. RDH16.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. intracellular membrane-bounded organelle Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671089.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Retinol dehydrogenase 16PRO_0000307693Add
BLAST

Post-translational modificationi

Not N-glycosylated.

Proteomic databases

PaxDbiO75452.
PRIDEiO75452.

PTM databases

PhosphoSiteiO75452.

Expressioni

Tissue specificityi

Highly expressed in adult liver (at protein level). Detected in endometrium, liver and foreskin. Detected in the spineous layers of adult skin, and at lower levels in basal and granular skin layers. Detected in fetal liver and lung.3 Publications

Inductioni

Transiently up-regulated by retinoic acid.1 Publication

Gene expression databases

BgeeiO75452.
CleanExiHS_RDH16.
GenevestigatoriO75452.

Organism-specific databases

HPAiHPA038518.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000381206.

Structurei

3D structure databases

ProteinModelPortaliO75452.
SMRiO75452. Positions 32-281.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 288288CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini310 – 3178LumenalSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei289 – 30921Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00760000118798.
HOVERGENiHBG005482.
InParanoidiO75452.
KOiK11154.
OMAiVKECVRD.
OrthoDBiEOG7FXZZX.
PhylomeDBiO75452.
TreeFamiTF325617.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75452-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWLYLAVFVG LYYLLHWYRE RQVLSHLRDK YVFITGCDSG FGKLLARQLD
60 70 80 90 100
ARGLRVLAAC LTEKGAEQLR GQTSDRLETV TLDVTKTESV AAAAQWVKEC
110 120 130 140 150
VRDKGLWGLV NNAGISLPTA PNELLTKQDF VTILDVNLLG VIDVTLSLLP
160 170 180 190 200
LVRRARGRVV NVSSVMGRVS LFGGGYCISK YGVEAFSDSL RRELSYFGVK
210 220 230 240 250
VAMIEPGYFK TAVTSKERFL KSFLEIWDRS SPEVKEAYGE KFVADYKKSA
260 270 280 290 300
EQMEQKCTQD LSLVTNCMEH ALIACHPRTR YSAGWDAKLL YLPMSYMPTF
310
LVDAIMYWVS PSPAKAL
Length:317
Mass (Da):35,673
Last modified:October 23, 2007 - v2
Checksum:iF9208C22B0515CFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1312FV → LL in AAC72923. (PubMed:10329026)Curated
Sequence conflicti147 – 1471S → N in AAC72923. (PubMed:10329026)Curated
Sequence conflicti154 – 1541R → K in AAC39922. (PubMed:9677409)Curated
Sequence conflicti163 – 1631S → F in AAC72923. (PubMed:10329026)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF057034 mRNA. Translation: AAC39922.1.
AF086735 mRNA. Translation: AAC72923.1.
CCDSiCCDS41797.1.
RefSeqiNP_003699.3. NM_003708.3.
UniGeneiHs.134958.

Genome annotation databases

EnsembliENST00000398138; ENSP00000381206; ENSG00000139547.
GeneIDi8608.
KEGGihsa:8608.
UCSCiuc001smi.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF057034 mRNA. Translation: AAC39922.1 .
AF086735 mRNA. Translation: AAC72923.1 .
CCDSi CCDS41797.1.
RefSeqi NP_003699.3. NM_003708.3.
UniGenei Hs.134958.

3D structure databases

ProteinModelPortali O75452.
SMRi O75452. Positions 32-281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000381206.

PTM databases

PhosphoSitei O75452.

Proteomic databases

PaxDbi O75452.
PRIDEi O75452.

Protocols and materials databases

DNASUi 8608.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000398138 ; ENSP00000381206 ; ENSG00000139547 .
GeneIDi 8608.
KEGGi hsa:8608.
UCSCi uc001smi.4. human.

Organism-specific databases

CTDi 8608.
GeneCardsi GC12M057346.
H-InvDB HIX0036688.
HGNCi HGNC:29674. RDH16.
HPAi HPA038518.
neXtProti NX_O75452.
PharmGKBi PA142671089.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1028.
GeneTreei ENSGT00760000118798.
HOVERGENi HBG005482.
InParanoidi O75452.
KOi K11154.
OMAi VKECVRD.
OrthoDBi EOG7FXZZX.
PhylomeDBi O75452.
TreeFami TF325617.

Enzyme and pathway databases

BioCyci MetaCyc:ENSG00000139547-MONOMER.
Reactomei REACT_160156. The canonical retinoid cycle in rods (twilight vision).
SABIO-RK O75452.

Miscellaneous databases

GenomeRNAii 8608.
NextBioi 32255.
PROi O75452.

Gene expression databases

Bgeei O75452.
CleanExi HS_RDH16.
Genevestigatori O75452.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning and characterization of a new human microsomal NAD+-dependent dehydrogenase that oxidizes all-trans-retinol and 3alpha-hydroxysteroids."
    Gough W.H., VanOoteghem S., Sint T., Kedishvili N.Y.
    J. Biol. Chem. 273:19778-19785(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "Cloning and characterization of retinol dehydrogenase transcripts expressed in human epidermal keratinocytes."
    Jurukovski V., Markova N.G., Karaman-Jurukovska N., Randolph R.K., Su J., Napoli J.L., Simon M.
    Mol. Genet. Metab. 67:62-73(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
    Tissue: Keratinocyte.
  3. "Expression of a retinol dehydrogenase (hRoDH-4), a member of the retinol/steroid dehydrogenase family implicated in retinoic acid biosynthesis, in normal and neoplastic endometria."
    Cain J.M., Zaino R., Shearer D., Bennett R.A., Olt G., Weisz J.
    Am. J. Obstet. Gynecol. 186:675-683(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4."
    Lapshina E.A., Belyaeva O.V., Chumakova O.V., Kedishvili N.Y.
    Biochemistry 42:776-784(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, LACK OF GLYCOSYLATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRDH16_HUMAN
AccessioniPrimary (citable) accession number: O75452
Secondary accession number(s): Q9UNV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3