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O75449

- KTNA1_HUMAN

UniProt

O75449 - KTNA1_HUMAN

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Protein
Katanin p60 ATPase-containing subunit A1
Gene
KATNA1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth By similarity.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Enzyme regulationi

ATPase activity is stimulated by microtubules, which promote homooligomerization By similarity. ATP dependent microtubule severing is stimulated by interaction with KATNB1.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2568ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. microtubule binding Source: UniProtKB
  3. microtubule-severing ATPase activity Source: UniProtKB-EC
  4. protein binding Source: UniProtKB
  5. protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  1. cytoplasmic microtubule organization Source: Ensembl
  2. microtubule bundle formation Source: Ensembl
  3. microtubule severing Source: UniProtKB-HAMAP
  4. mitotic interphase Source: UniProtKB
  5. mitotic nuclear division Source: UniProtKB-KW
  6. negative regulation of neuron projection development Source: Ensembl
  7. neuron migration Source: Ensembl
  8. protein localization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p60 ATPase-containing subunit A1 (EC:3.6.4.3)
Short name:
Katanin p60 subunit A1
Alternative name(s):
p60 katanin
Gene namesi
Name:KATNA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:6216. KATNA1.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle. Cytoplasmcytoskeletonspindle pole. Midbody. Cytoplasmcytoskeleton
Note: Predominantly cytoplasmic. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.2 Publications

GO - Cellular componenti

  1. axon Source: Ensembl
  2. centrosome Source: UniProtKB-HAMAP
  3. cytoplasm Source: UniProtKB
  4. growth cone Source: Ensembl
  5. lipid particle Source: Ensembl
  6. microtubule Source: UniProtKB-KW
  7. midbody Source: UniProtKB
  8. neuronal cell body Source: Ensembl
  9. nucleus Source: Ensembl
  10. plasma membrane Source: Ensembl
  11. spindle Source: UniProtKB
  12. spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi255 – 2551K → A: Abolishes ATP dependent microtubule severing activity and localization to spindle poles. 2 Publications
Mutagenesisi308 – 3081D → N: Abolishes ATP dependent microtubule severing activity and localization to spindle poles; when associated with N-309. 1 Publication
Mutagenesisi309 – 3091E → N: Abolishes ATP dependent microtubule severing activity and localization to spindle poles; when associated with N-308. 1 Publication

Organism-specific databases

PharmGKBiPA30017.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Katanin p60 ATPase-containing subunit A1UniRule annotation
PRO_0000084594Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421Phosphoserine; by DYRK21 Publication
Modified residuei109 – 1091Phosphoserine; by DYRK21 Publication
Modified residuei133 – 1331Phosphothreonine; by DYRK21 Publication
Modified residuei170 – 1701Phosphoserine2 Publications

Post-translational modificationi

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.UniRule annotation
Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted to proteasomal degradation. Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO75449.
PaxDbiO75449.
PRIDEiO75449.

PTM databases

PhosphoSiteiO75449.

Expressioni

Gene expression databases

ArrayExpressiO75449.
BgeeiO75449.
CleanExiHS_KATNA1.
GenevestigatoriO75449.

Organism-specific databases

HPAiHPA036207.

Interactioni

Subunit structurei

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules By similarity. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with dynein and NDEL1 By similarity. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42.2 Publications

Protein-protein interaction databases

BioGridi116285. 21 interactions.
STRINGi9606.ENSP00000335106.

Structurei

3D structure databases

ProteinModelPortaliO75449.
SMRiO75449. Positions 1-72, 166-472.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 185185Interaction with microtubulesUniRule annotation
Add
BLAST
Regioni1 – 7575Interaction with dynein and NDEL1 By similarity
Add
BLAST
Regioni1 – 2929Interaction with KATNB1UniRule annotation
Add
BLAST

Domaini

The N-terminus is sufficient for interaction with microtubules. However, high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000225142.
HOVERGENiHBG057074.
InParanoidiO75449.
KOiK07767.
OMAiSQYSDPK.
OrthoDBiEOG7SFHWZ.
PhylomeDBiO75449.
TreeFamiTF323170.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03023. Katanin_p60_A1.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O75449-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSLLMISENV KLAREYALLG NYDSAMVYYQ GVLDQMNKYL YSVKDTYLQQ    50
KWQQVWQEIN VEAKHVKDIM KTLESFKLDS TPLKAAQHDL PASEGEVWSM 100
PVPVERRPSP GPRKRQSSQY SDPKSHGNRP STTVRVHRSS AQNVHNDRGK 150
AVRCREKKEQ NKGREEKNKS PAAVTEPETN KFDSTGYDKD LVEALERDII 200
SQNPNVRWDD IADLVEAKKL LKEAVVLPMW MPEFFKGIRR PWKGVLMVGP 250
PGTGKTLLAK AVATECKTTF FNVSSSTLTS KYRGESEKLV RLLFEMARFY 300
SPATIFIDEI DSICSRRGTS EEHEASRRVK AELLVQMDGV GGTSENDDPS 350
KMVMVLAATN FPWDIDEALR RRLEKRIYIP LPSAKGREEL LRISLRELEL 400
ADDVDLASIA ENMEGYSGAD ITNVCRDASL MAMRRRIEGL TPEEIRNLSK 450
EEMHMPTTME DFEMALKKVS KSVSAADIER YEKWIFEFGS C 491
Length:491
Mass (Da):55,965
Last modified:November 1, 1998 - v1
Checksum:iF431594F478491DD
GO
Isoform 2 (identifier: O75449-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: Missing.
     384-387: AKGR → GMRP
     388-491: Missing.

Note: No experimental confirmation available.

Show »
Length:311
Mass (Da):35,319
Checksum:i9DF635C4E51147C7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 24376Missing in isoform 2.
VSP_012948Add
BLAST
Alternative sequencei384 – 3874AKGR → GMRP in isoform 2.
VSP_012949
Alternative sequencei388 – 491104Missing in isoform 2.
VSP_012950Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF056022 mRNA. Translation: AAC25114.1.
AL078581, BX276089 Genomic DNA. Translation: CAI19504.1.
AL078581, BX276089 Genomic DNA. Translation: CAI19505.1.
BX276089, AL078581 Genomic DNA. Translation: CAI16431.1.
BX276089, AL078581 Genomic DNA. Translation: CAI16432.1.
CH471051 Genomic DNA. Translation: EAW47793.1.
CH471051 Genomic DNA. Translation: EAW47795.1.
BC050428 mRNA. Translation: AAH50428.1.
CCDSiCCDS5217.1. [O75449-1]
CCDS56456.1. [O75449-2]
RefSeqiNP_001191005.1. NM_001204076.1. [O75449-2]
NP_008975.1. NM_007044.3. [O75449-1]
XP_005266861.1. XM_005266804.1. [O75449-1]
UniGeneiHs.450175.

Genome annotation databases

EnsembliENST00000335643; ENSP00000335180; ENSG00000186625. [O75449-2]
ENST00000335647; ENSP00000335106; ENSG00000186625. [O75449-1]
ENST00000367411; ENSP00000356381; ENSG00000186625. [O75449-1]
GeneIDi11104.
KEGGihsa:11104.
UCSCiuc003qmr.2. human. [O75449-1]
uc003qmt.3. human. [O75449-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF056022 mRNA. Translation: AAC25114.1 .
AL078581 , BX276089 Genomic DNA. Translation: CAI19504.1 .
AL078581 , BX276089 Genomic DNA. Translation: CAI19505.1 .
BX276089 , AL078581 Genomic DNA. Translation: CAI16431.1 .
BX276089 , AL078581 Genomic DNA. Translation: CAI16432.1 .
CH471051 Genomic DNA. Translation: EAW47793.1 .
CH471051 Genomic DNA. Translation: EAW47795.1 .
BC050428 mRNA. Translation: AAH50428.1 .
CCDSi CCDS5217.1. [O75449-1 ]
CCDS56456.1. [O75449-2 ]
RefSeqi NP_001191005.1. NM_001204076.1. [O75449-2 ]
NP_008975.1. NM_007044.3. [O75449-1 ]
XP_005266861.1. XM_005266804.1. [O75449-1 ]
UniGenei Hs.450175.

3D structure databases

ProteinModelPortali O75449.
SMRi O75449. Positions 1-72, 166-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116285. 21 interactions.
STRINGi 9606.ENSP00000335106.

PTM databases

PhosphoSitei O75449.

Proteomic databases

MaxQBi O75449.
PaxDbi O75449.
PRIDEi O75449.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000335643 ; ENSP00000335180 ; ENSG00000186625 . [O75449-2 ]
ENST00000335647 ; ENSP00000335106 ; ENSG00000186625 . [O75449-1 ]
ENST00000367411 ; ENSP00000356381 ; ENSG00000186625 . [O75449-1 ]
GeneIDi 11104.
KEGGi hsa:11104.
UCSCi uc003qmr.2. human. [O75449-1 ]
uc003qmt.3. human. [O75449-2 ]

Organism-specific databases

CTDi 11104.
GeneCardsi GC06M149957.
HGNCi HGNC:6216. KATNA1.
HPAi HPA036207.
MIMi 606696. gene.
neXtProti NX_O75449.
PharmGKBi PA30017.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0464.
HOGENOMi HOG000225142.
HOVERGENi HBG057074.
InParanoidi O75449.
KOi K07767.
OMAi SQYSDPK.
OrthoDBi EOG7SFHWZ.
PhylomeDBi O75449.
TreeFami TF323170.

Miscellaneous databases

ChiTaRSi KATNA1. human.
GeneWikii KATNA1.
GenomeRNAii 11104.
NextBioi 42220.
PROi O75449.
SOURCEi Search...

Gene expression databases

ArrayExpressi O75449.
Bgeei O75449.
CleanExi HS_KATNA1.
Genevestigatori O75449.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_03023. Katanin_p60_A1.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Katanin is responsible for the M-phase microtubule-severing activity in Xenopus eggs."
    McNally F.J., Thomas S.
    Mol. Biol. Cell 9:1847-1861(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
  2. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  5. "Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin."
    McNally K.P., Bazirgan O.A., McNally F.J.
    J. Cell Sci. 113:1623-1633(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KATNB1, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-255; ASP-308 AND GLU-309.
  6. "Katanin inhibition prevents the redistribution of gamma-tubulin at mitosis."
    Buster D., McNally K., McNally F.J.
    J. Cell Sci. 115:1083-1092(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-255.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cells."
    Cummings C.M., Bentley C.A., Perdue S.A., Baas P.W., Singer J.D.
    J. Biol. Chem. 284:11663-11675(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION BY THE BCR(KLHL42) COMPLEX.
  9. "Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase."
    Maddika S., Chen J.
    Nat. Cell Biol. 11:409-419(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-42; SER-109 AND THR-133 BY DYRK2, FUNCTION AS KATNA1 KINASE, INTERACTION WITH EDVP COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKTNA1_HUMAN
AccessioniPrimary (citable) accession number: O75449
Secondary accession number(s): E1P5A3
, Q5TFA8, Q5TFA9, Q86VN2, Q9NU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1998
Last modified: September 3, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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