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Protein

Katanin p60 ATPase-containing subunit A1

Gene

KATNA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Enzyme regulationi

ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi249 – 256ATPUniRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB
  • microtubule-severing ATPase activity Source: UniProtKB-EC
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS03490-MONOMER.
BRENDAi3.6.4.3. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p60 ATPase-containing subunit A1UniRule annotation (EC:3.6.4.3UniRule annotation)
Short name:
Katanin p60 subunit A1UniRule annotation
Alternative name(s):
p60 kataninUniRule annotation
Gene namesi
Name:KATNA1UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:6216. KATNA1.

Subcellular locationi

  • Cytoplasm
  • Midbody
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation
  • Cytoplasmcytoskeletonspindle pole

  • Note: Predominantly cytoplasmic. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.

GO - Cellular componenti

  • centrosome Source: UniProtKB-HAMAP
  • cytoplasm Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • microtubule organizing center Source: HPA
  • midbody Source: UniProtKB
  • nucleus Source: HPA
  • spindle Source: UniProtKB
  • spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi255K → A: Abolishes ATP dependent microtubule severing activity and localization to spindle poles. 2 Publications1
Mutagenesisi308D → N: Abolishes ATP dependent microtubule severing activity and localization to spindle poles; when associated with N-309. 1 Publication1
Mutagenesisi309E → N: Abolishes ATP dependent microtubule severing activity and localization to spindle poles; when associated with N-308. 1 Publication1

Organism-specific databases

DisGeNETi11104.
OpenTargetsiENSG00000186625.
PharmGKBiPA30017.

Polymorphism and mutation databases

BioMutaiKATNA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845941 – 491Katanin p60 ATPase-containing subunit A1Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42Phosphoserine; by DYRK2UniRule annotation1 Publication1
Modified residuei109Phosphoserine; by DYRK2UniRule annotation1 Publication1
Modified residuei133Phosphothreonine; by DYRK2UniRule annotation1 Publication1
Modified residuei170PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.UniRule annotation2 Publications
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.UniRule annotation1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75449.
MaxQBiO75449.
PaxDbiO75449.
PeptideAtlasiO75449.
PRIDEiO75449.

PTM databases

iPTMnetiO75449.
PhosphoSitePlusiO75449.

Expressioni

Gene expression databases

BgeeiENSG00000186625.
CleanExiHS_KATNA1.
ExpressionAtlasiO75449. baseline and differential.
GenevisibleiO75449. HS.

Organism-specific databases

HPAiHPA036207.

Interactioni

Subunit structurei

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules (By similarity). Interacts with KATNB1, which may serve as a targeting subunit. Interacts with dynein and NDEL1 (By similarity). Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42.UniRule annotation2 Publications

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi116285. 49 interactors.
IntActiO75449. 24 interactors.
STRINGi9606.ENSP00000335106.

Structurei

3D structure databases

ProteinModelPortaliO75449.
SMRiO75449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 185Interaction with microtubulesAdd BLAST185
Regioni1 – 75Interaction with dynein and NDEL1By similarityAdd BLAST75
Regioni1 – 29Interaction with KATNB11 PublicationAdd BLAST29

Domaini

The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0738. Eukaryota.
ENOG410XPN7. LUCA.
GeneTreeiENSGT00550000074466.
HOGENOMiHOG000225142.
HOVERGENiHBG057074.
InParanoidiO75449.
KOiK07767.
OMAiSQYSDPK.
OrthoDBiEOG091G0Q8J.
PhylomeDBiO75449.
TreeFamiTF323170.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03023. Katanin_p60_A1. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75449-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLMISENV KLAREYALLG NYDSAMVYYQ GVLDQMNKYL YSVKDTYLQQ
60 70 80 90 100
KWQQVWQEIN VEAKHVKDIM KTLESFKLDS TPLKAAQHDL PASEGEVWSM
110 120 130 140 150
PVPVERRPSP GPRKRQSSQY SDPKSHGNRP STTVRVHRSS AQNVHNDRGK
160 170 180 190 200
AVRCREKKEQ NKGREEKNKS PAAVTEPETN KFDSTGYDKD LVEALERDII
210 220 230 240 250
SQNPNVRWDD IADLVEAKKL LKEAVVLPMW MPEFFKGIRR PWKGVLMVGP
260 270 280 290 300
PGTGKTLLAK AVATECKTTF FNVSSSTLTS KYRGESEKLV RLLFEMARFY
310 320 330 340 350
SPATIFIDEI DSICSRRGTS EEHEASRRVK AELLVQMDGV GGTSENDDPS
360 370 380 390 400
KMVMVLAATN FPWDIDEALR RRLEKRIYIP LPSAKGREEL LRISLRELEL
410 420 430 440 450
ADDVDLASIA ENMEGYSGAD ITNVCRDASL MAMRRRIEGL TPEEIRNLSK
460 470 480 490
EEMHMPTTME DFEMALKKVS KSVSAADIER YEKWIFEFGS C
Length:491
Mass (Da):55,965
Last modified:November 1, 1998 - v1
Checksum:iF431594F478491DD
GO
Isoform 2 (identifier: O75449-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-243: Missing.
     384-387: AKGR → GMRP
     388-491: Missing.

Note: No experimental confirmation available.
Show »
Length:311
Mass (Da):35,319
Checksum:i9DF635C4E51147C7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012948168 – 243Missing in isoform 2. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_012949384 – 387AKGR → GMRP in isoform 2. 1 Publication4
Alternative sequenceiVSP_012950388 – 491Missing in isoform 2. 1 PublicationAdd BLAST104

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056022 mRNA. Translation: AAC25114.1.
AL078581, BX276089 Genomic DNA. Translation: CAI19504.1.
AL078581, BX276089 Genomic DNA. Translation: CAI19505.1.
BX276089, AL078581 Genomic DNA. Translation: CAI16431.1.
BX276089, AL078581 Genomic DNA. Translation: CAI16432.1.
CH471051 Genomic DNA. Translation: EAW47793.1.
CH471051 Genomic DNA. Translation: EAW47795.1.
BC050428 mRNA. Translation: AAH50428.1.
CCDSiCCDS5217.1. [O75449-1]
CCDS56456.1. [O75449-2]
RefSeqiNP_001191005.1. NM_001204076.1. [O75449-2]
NP_008975.1. NM_007044.3. [O75449-1]
XP_005266861.1. XM_005266804.2. [O75449-1]
XP_016865696.1. XM_017010207.1. [O75449-1]
XP_016865699.1. XM_017010210.1. [O75449-1]
UniGeneiHs.450175.

Genome annotation databases

EnsembliENST00000335643; ENSP00000335180; ENSG00000186625. [O75449-2]
ENST00000335647; ENSP00000335106; ENSG00000186625. [O75449-1]
ENST00000367411; ENSP00000356381; ENSG00000186625. [O75449-1]
GeneIDi11104.
KEGGihsa:11104.
UCSCiuc003qmr.3. human. [O75449-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056022 mRNA. Translation: AAC25114.1.
AL078581, BX276089 Genomic DNA. Translation: CAI19504.1.
AL078581, BX276089 Genomic DNA. Translation: CAI19505.1.
BX276089, AL078581 Genomic DNA. Translation: CAI16431.1.
BX276089, AL078581 Genomic DNA. Translation: CAI16432.1.
CH471051 Genomic DNA. Translation: EAW47793.1.
CH471051 Genomic DNA. Translation: EAW47795.1.
BC050428 mRNA. Translation: AAH50428.1.
CCDSiCCDS5217.1. [O75449-1]
CCDS56456.1. [O75449-2]
RefSeqiNP_001191005.1. NM_001204076.1. [O75449-2]
NP_008975.1. NM_007044.3. [O75449-1]
XP_005266861.1. XM_005266804.2. [O75449-1]
XP_016865696.1. XM_017010207.1. [O75449-1]
XP_016865699.1. XM_017010210.1. [O75449-1]
UniGeneiHs.450175.

3D structure databases

ProteinModelPortaliO75449.
SMRiO75449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116285. 49 interactors.
IntActiO75449. 24 interactors.
STRINGi9606.ENSP00000335106.

PTM databases

iPTMnetiO75449.
PhosphoSitePlusiO75449.

Polymorphism and mutation databases

BioMutaiKATNA1.

Proteomic databases

EPDiO75449.
MaxQBiO75449.
PaxDbiO75449.
PeptideAtlasiO75449.
PRIDEiO75449.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335643; ENSP00000335180; ENSG00000186625. [O75449-2]
ENST00000335647; ENSP00000335106; ENSG00000186625. [O75449-1]
ENST00000367411; ENSP00000356381; ENSG00000186625. [O75449-1]
GeneIDi11104.
KEGGihsa:11104.
UCSCiuc003qmr.3. human. [O75449-1]

Organism-specific databases

CTDi11104.
DisGeNETi11104.
GeneCardsiKATNA1.
HGNCiHGNC:6216. KATNA1.
HPAiHPA036207.
MIMi606696. gene.
neXtProtiNX_O75449.
OpenTargetsiENSG00000186625.
PharmGKBiPA30017.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0738. Eukaryota.
ENOG410XPN7. LUCA.
GeneTreeiENSGT00550000074466.
HOGENOMiHOG000225142.
HOVERGENiHBG057074.
InParanoidiO75449.
KOiK07767.
OMAiSQYSDPK.
OrthoDBiEOG091G0Q8J.
PhylomeDBiO75449.
TreeFamiTF323170.

Enzyme and pathway databases

BioCyciZFISH:HS03490-MONOMER.
BRENDAi3.6.4.3. 2681.

Miscellaneous databases

ChiTaRSiKATNA1. human.
GeneWikiiKATNA1.
GenomeRNAii11104.
PROiO75449.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186625.
CleanExiHS_KATNA1.
ExpressionAtlasiO75449. baseline and differential.
GenevisibleiO75449. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03023. Katanin_p60_A1. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKTNA1_HUMAN
AccessioniPrimary (citable) accession number: O75449
Secondary accession number(s): E1P5A3
, Q5TFA8, Q5TFA9, Q86VN2, Q9NU52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.