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Protein

Mediator of RNA polymerase II transcription subunit 24

Gene

MED24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.2 Publications

GO - Molecular functioni

  • histone acetyltransferase activity Source: Ensembl
  • ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  • receptor activity Source: UniProtKB
  • RNA polymerase II transcription cofactor activity Source: UniProtKB
  • thyroid hormone receptor binding Source: UniProtKB
  • transcription cofactor activity Source: UniProtKB
  • ubiquitin protein ligase activity Source: Ensembl
  • vitamin D receptor binding Source: UniProtKB

GO - Biological processi

  • androgen receptor signaling pathway Source: UniProtKB
  • intracellular steroid hormone receptor signaling pathway Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • protein heterooligomerization Source: Ensembl
  • stem cell population maintenance Source: Ensembl
  • transcription initiation from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000008838-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 24
Alternative name(s):
Activator-recruited cofactor 100 kDa component
Short name:
ARC100
Cofactor required for Sp1 transcriptional activation subunit 4
Short name:
CRSP complex subunit 4
Mediator complex subunit 24
Thyroid hormone receptor-associated protein 4
Thyroid hormone receptor-associated protein complex 100 kDa component
Short name:
Trap100
Short name:
hTRAP100
Vitamin D3 receptor-interacting protein complex 100 kDa component
Short name:
DRIP100
Gene namesi
Name:MED24
Synonyms:ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:22963. MED24.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9862.
OpenTargetsiENSG00000008838.
PharmGKBiPA162395566.

Polymorphism and mutation databases

BioMutaiMED24.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000655821 – 989Mediator of RNA polymerase II transcription subunit 24Add BLAST989

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei862PhosphoserineCombined sources1
Modified residuei873PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75448.
MaxQBiO75448.
PaxDbiO75448.
PeptideAtlasiO75448.
PRIDEiO75448.

PTM databases

iPTMnetiO75448.
PhosphoSitePlusiO75448.

Expressioni

Tissue specificityi

Ubiquitous. Abundant in skeletal muscle, heart and placenta.1 Publication

Gene expression databases

BgeeiENSG00000008838.
CleanExiHS_MED24.
ExpressionAtlasiO75448. baseline and differential.
GenevisibleiO75448. HS.

Organism-specific databases

HPAiCAB025177.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED16Q9Y2X02EBI-394523,EBI-394541

GO - Molecular functioni

  • thyroid hormone receptor binding Source: UniProtKB
  • vitamin D receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115196. 78 interactors.
DIPiDIP-31462N.
IntActiO75448. 36 interactors.
MINTiMINT-244199.
STRINGi9606.ENSP00000377686.

Structurei

3D structure databases

ProteinModelPortaliO75448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi128 – 132LXXLL motif 15
Motifi344 – 348LXXLL motif 25
Motifi448 – 452LXXLL motif 35
Motifi557 – 561LXXLL motif 45
Motifi788 – 792LXXLL motif 55
Motifi857 – 861LXXLL motif 65

Sequence similaritiesi

Belongs to the Mediator complex subunit 24 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF72. Eukaryota.
ENOG410YPDT. LUCA.
GeneTreeiENSGT00390000016438.
HOGENOMiHOG000293419.
HOVERGENiHBG055588.
InParanoidiO75448.
KOiK15167.
PhylomeDBiO75448.
TreeFamiTF323565.

Family and domain databases

InterProiIPR021429. Mediator_Med24_N.
[Graphical view]
PfamiPF11277. Med24_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75448-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLADALLEQA
60 70 80 90 100
MIGPSPNPLI LSYLKYAISS QMVSYSSVLT AISKFDDFSR DLCVQALLDI
110 120 130 140 150
MDMFCDRLSC HGKAEECIGL CRALLSALHW LLRCTAASAE RLREGLEAGT
160 170 180 190 200
PAAGEKQLAM CLQRLEKTLS STKNRALLHI AKLEEASSWT AIEHSLLKLG
210 220 230 240 250
EILANLSNPQ LRSQAEQCGT LIRSIPTMLS VHAEQMHKTG FPTVHAVILL
260 270 280 290 300
EGTMNLTGET QSLVEQLTMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT
310 320 330 340 350
EELKWTAFTF LKIPQVLVKL KKYSHGDKDF TEDVNCAFEF LLKLTPLLDK
360 370 380 390 400
ADQRCNCDCT NFLLQECGKQ GLLSEASVNN LMAKRKADRE HAPQQKSGEN
410 420 430 440 450
ANIQPNIQLI LRAEPTVTNI LKTMDADHSK SPEGLLGVLG HMLSGKSLDL
460 470 480 490 500
LLAAAAATGK LKSFARKFIN LNEFTTYGSE ESTKPASVRA LLFDISFLML
510 520 530 540 550
CHVAQTYGSE VILSESRTGA EVPFFETWMQ TCMPEEGKIL NPDHPCFRPD
560 570 580 590 600
STKVESLVAL LNNSSEMKLV QMKWHEACLS ISAAILEILN AWENGVLAFE
610 620 630 640 650
SIQKITDNIK GKVCSLAVCA VAWLVAHVRM LGLDEREKSL QMIRQLAGPL
660 670 680 690 700
FSENTLQFYN ERVVIMNSIL ERMCADVLQQ TATQIKFPST GVDTMPYWNL
710 720 730 740 750
LPPKRPIKEV LTDIFAKVLE KGWVDSRSIH IFDTLLHMGG VYWFCNNLIK
760 770 780 790 800
ELLKETRKEH TLRAVELLYS IFCLDMQQVT LVLLGHILPG LLTDSSKWHS
810 820 830 840 850
LMDPPGTALA KLAVWCALSS YSSHKGQAST RQKKRHREDI EDYISLFPLD
860 870 880 890 900
DVQPSKLMRL LSSNEDDANI LSSPTDRSMS SSLSASQLHT VNMRDPLNRV
910 920 930 940 950
LANLFLLISS ILGSRTAGPH TQFVQWFMEE CVDCLEQGGR GSVLQFMPFT
960 970 980
TVSELVKVSA MSSPKVVLAI TDLSLPLGRQ VAAKAIAAL
Length:989
Mass (Da):110,305
Last modified:November 1, 1998 - v1
Checksum:iCCEDE7D4E74D890C
GO
Isoform 2 (identifier: O75448-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-84: Missing.

Show »
Length:976
Mass (Da):108,938
Checksum:iC3628AD29869DF3C
GO

Sequence cautioni

The sequence BAA09479 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20D → Y in BAA09479 (PubMed:8590280).Curated1
Sequence conflicti476Missing AA sequence (PubMed:10235266).Curated1
Sequence conflicti555E → G in AAF78764 (PubMed:9637681).Curated1
Sequence conflicti710V → E in BAG51081 (PubMed:14702039).Curated1
Sequence conflicti741V → A in BAG51081 (PubMed:14702039).Curated1
Sequence conflicti969A → P in BAG51081 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053969204A → T.1 PublicationCorresponds to variant rs34585432dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04112572 – 84Missing in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055995 mRNA. Translation: AAC39855.1.
AF277379 mRNA. Translation: AAF78764.1.
D50920 mRNA. Translation: BAA09479.2. Different initiation.
AK022508 mRNA. Translation: BAG51081.1.
AK291040 mRNA. Translation: BAF83729.1.
AC090844 Genomic DNA. No translation available.
AC102799 Genomic DNA. No translation available.
BC011375 mRNA. Translation: AAH11375.1.
CCDSiCCDS11359.1. [O75448-1]
CCDS42315.1. [O75448-2]
RefSeqiNP_001072986.1. NM_001079518.1. [O75448-2]
NP_001254726.1. NM_001267797.1. [O75448-2]
NP_055630.2. NM_014815.3. [O75448-1]
XP_016880955.1. XM_017025466.1. [O75448-1]
UniGeneiHs.462983.

Genome annotation databases

EnsembliENST00000356271; ENSP00000348610; ENSG00000008838. [O75448-2]
ENST00000394127; ENSP00000377685; ENSG00000008838. [O75448-2]
ENST00000394128; ENSP00000377686; ENSG00000008838. [O75448-1]
GeneIDi9862.
KEGGihsa:9862.
UCSCiuc002htt.4. human. [O75448-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055995 mRNA. Translation: AAC39855.1.
AF277379 mRNA. Translation: AAF78764.1.
D50920 mRNA. Translation: BAA09479.2. Different initiation.
AK022508 mRNA. Translation: BAG51081.1.
AK291040 mRNA. Translation: BAF83729.1.
AC090844 Genomic DNA. No translation available.
AC102799 Genomic DNA. No translation available.
BC011375 mRNA. Translation: AAH11375.1.
CCDSiCCDS11359.1. [O75448-1]
CCDS42315.1. [O75448-2]
RefSeqiNP_001072986.1. NM_001079518.1. [O75448-2]
NP_001254726.1. NM_001267797.1. [O75448-2]
NP_055630.2. NM_014815.3. [O75448-1]
XP_016880955.1. XM_017025466.1. [O75448-1]
UniGeneiHs.462983.

3D structure databases

ProteinModelPortaliO75448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115196. 78 interactors.
DIPiDIP-31462N.
IntActiO75448. 36 interactors.
MINTiMINT-244199.
STRINGi9606.ENSP00000377686.

PTM databases

iPTMnetiO75448.
PhosphoSitePlusiO75448.

Polymorphism and mutation databases

BioMutaiMED24.

Proteomic databases

EPDiO75448.
MaxQBiO75448.
PaxDbiO75448.
PeptideAtlasiO75448.
PRIDEiO75448.

Protocols and materials databases

DNASUi9862.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356271; ENSP00000348610; ENSG00000008838. [O75448-2]
ENST00000394127; ENSP00000377685; ENSG00000008838. [O75448-2]
ENST00000394128; ENSP00000377686; ENSG00000008838. [O75448-1]
GeneIDi9862.
KEGGihsa:9862.
UCSCiuc002htt.4. human. [O75448-1]

Organism-specific databases

CTDi9862.
DisGeNETi9862.
GeneCardsiMED24.
H-InvDBHIX0174136.
HGNCiHGNC:22963. MED24.
HPAiCAB025177.
MIMi607000. gene.
neXtProtiNX_O75448.
OpenTargetsiENSG00000008838.
PharmGKBiPA162395566.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF72. Eukaryota.
ENOG410YPDT. LUCA.
GeneTreeiENSGT00390000016438.
HOGENOMiHOG000293419.
HOVERGENiHBG055588.
InParanoidiO75448.
KOiK15167.
PhylomeDBiO75448.
TreeFamiTF323565.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000008838-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED24. human.
GeneWikiiMED24.
GenomeRNAii9862.
PROiO75448.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000008838.
CleanExiHS_MED24.
ExpressionAtlasiO75448. baseline and differential.
GenevisibleiO75448. HS.

Family and domain databases

InterProiIPR021429. Mediator_Med24_N.
[Graphical view]
PfamiPF11277. Med24_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED24_HUMAN
AccessioniPrimary (citable) accession number: O75448
Secondary accession number(s): A8K4S5
, B3KMR9, Q14143, Q9NNY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2002
Last sequence update: November 1, 1998
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.