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O75446

- SAP30_HUMAN

UniProt

O75446 - SAP30_HUMAN

Protein

Histone deacetylase complex subunit SAP30

Gene

SAP30

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri67 – 11549AtypicalAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. transcription corepressor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    2. regulation of transcription, DNA-templated Source: UniProtKB
    3. skeletal muscle cell differentiation Source: Ensembl
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_200856. NoRC negatively regulates rRNA expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone deacetylase complex subunit SAP30
    Alternative name(s):
    30 kDa Sin3-associated polypeptide
    Sin3 corepressor complex subunit SAP30
    Sin3-associated polypeptide p30
    Gene namesi
    Name:SAP30Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:10532. SAP30.

    Subcellular locationi

    GO - Cellular componenti

    1. histone deacetylase complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi67 – 671C → A: Abolishes zinc-binding. 1 Publication
    Mutagenesisi68 – 681C → A: Retains zinc-binding. 1 Publication
    Mutagenesisi108 – 1081H → A: Retains zinc-binding. 1 Publication
    Mutagenesisi112 – 1121C → A: Abolishes zinc-binding. 1 Publication

    Organism-specific databases

    PharmGKBiPA34941.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220Histone deacetylase complex subunit SAP30PRO_0000097582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei131 – 1311Phosphoserine5 Publications
    Modified residuei138 – 1381Phosphoserine4 Publications
    Modified residuei145 – 1451Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO75446.
    PaxDbiO75446.
    PeptideAtlasiO75446.
    PRIDEiO75446.

    PTM databases

    PhosphoSiteiO75446.

    Expressioni

    Tissue specificityi

    Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.1 Publication

    Gene expression databases

    BgeeiO75446.
    CleanExiHS_SAP30.
    GenevestigatoriO75446.

    Interactioni

    Subunit structurei

    Interacts with SIN3A, SIN3B, HDAC2 and NCOR1. Interacts with SAMS1 By similarity. Component of the histone deacetylase complex that includes at least SIN3A, HDAC1 and HDAC2. Interacts with HDAC1 and HCFC1.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi114346. 33 interactions.
    IntActiO75446. 8 interactions.
    MINTiMINT-133479.
    STRINGi9606.ENSP00000296504.

    Structurei

    Secondary structure

    1
    220
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi64 – 663
    Beta strandi69 – 713
    Helixi87 – 9610
    Beta strandi99 – 1024
    Helixi113 – 1208
    Beta strandi123 – 1253

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KDPNMR-A64-131[»]
    ProteinModelPortaliO75446.
    SMRiO75446. Positions 64-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO75446.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 129129Interaction with NCOR1By similarityAdd
    BLAST
    Regioni130 – 22091Interaction with SIN3ABy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi15 – 5945Ala-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SAP30 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri67 – 11549AtypicalAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG81414.
    HOGENOMiHOG000007811.
    HOVERGENiHBG057907.
    InParanoidiO75446.
    OMAiKIDSGVH.
    OrthoDBiEOG7P5T35.
    PhylomeDBiO75446.
    TreeFamiTF324135.

    Family and domain databases

    InterProiIPR024145. His_deAcase_SAP30/SAP30L.
    IPR025718. SAP30_Sin3-bd.
    IPR025717. SAP30_zn-finger.
    [Graphical view]
    PANTHERiPTHR13286. PTHR13286. 1 hit.
    PfamiPF13867. SAP30_Sin3_bdg. 1 hit.
    PF13866. zf-SAP30. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O75446-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGFTPDEMS RGGDAAAAVA AVVAAAAAAA SAGNGTGAGT GAEVPGAGAV    50
    SAAGPPGAAG PGPGQLCCLR EDGERCGRAA GNASFSKRIQ KSISQKKVKI 100
    ELDKSARHLY ICDYHKNLIQ SVRNRRKRKG SDDDGGDSPV QDIDTPEVDL 150
    YQLQVNTLRR YKRHFKLPTR PGLNKAQLVE IVGCHFRSIP VNEKDTLTYF 200
    IYSVKNDKNK SDLKVDSGVH 220
    Length:220
    Mass (Da):23,306
    Last modified:November 1, 1998 - v1
    Checksum:iFF84E06B17AFCAC2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055993 mRNA. Translation: AAC33316.1.
    CH471056 Genomic DNA. Translation: EAX04755.1.
    CH471056 Genomic DNA. Translation: EAX04756.1.
    BC016757 mRNA. Translation: AAH16757.1.
    CCDSiCCDS3817.1.
    RefSeqiNP_003855.1. NM_003864.3.
    UniGeneiHs.591715.

    Genome annotation databases

    EnsembliENST00000296504; ENSP00000296504; ENSG00000164105.
    GeneIDi8819.
    KEGGihsa:8819.
    UCSCiuc003itd.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055993 mRNA. Translation: AAC33316.1 .
    CH471056 Genomic DNA. Translation: EAX04755.1 .
    CH471056 Genomic DNA. Translation: EAX04756.1 .
    BC016757 mRNA. Translation: AAH16757.1 .
    CCDSi CCDS3817.1.
    RefSeqi NP_003855.1. NM_003864.3.
    UniGenei Hs.591715.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KDP NMR - A 64-131 [» ]
    ProteinModelPortali O75446.
    SMRi O75446. Positions 64-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114346. 33 interactions.
    IntActi O75446. 8 interactions.
    MINTi MINT-133479.
    STRINGi 9606.ENSP00000296504.

    PTM databases

    PhosphoSitei O75446.

    Proteomic databases

    MaxQBi O75446.
    PaxDbi O75446.
    PeptideAtlasi O75446.
    PRIDEi O75446.

    Protocols and materials databases

    DNASUi 8819.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296504 ; ENSP00000296504 ; ENSG00000164105 .
    GeneIDi 8819.
    KEGGi hsa:8819.
    UCSCi uc003itd.3. human.

    Organism-specific databases

    CTDi 8819.
    GeneCardsi GC04P174292.
    HGNCi HGNC:10532. SAP30.
    MIMi 603378. gene.
    neXtProti NX_O75446.
    PharmGKBi PA34941.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG81414.
    HOGENOMi HOG000007811.
    HOVERGENi HBG057907.
    InParanoidi O75446.
    OMAi KIDSGVH.
    OrthoDBi EOG7P5T35.
    PhylomeDBi O75446.
    TreeFami TF324135.

    Enzyme and pathway databases

    Reactomei REACT_200856. NoRC negatively regulates rRNA expression.

    Miscellaneous databases

    EvolutionaryTracei O75446.
    GeneWikii SAP30.
    GenomeRNAii 8819.
    NextBioi 33082.
    PROi O75446.
    SOURCEi Search...

    Gene expression databases

    Bgeei O75446.
    CleanExi HS_SAP30.
    Genevestigatori O75446.

    Family and domain databases

    InterProi IPR024145. His_deAcase_SAP30/SAP30L.
    IPR025718. SAP30_Sin3-bd.
    IPR025717. SAP30_zn-finger.
    [Graphical view ]
    PANTHERi PTHR13286. PTHR13286. 1 hit.
    Pfami PF13867. SAP30_Sin3_bdg. 1 hit.
    PF13866. zf-SAP30. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex."
      Zhang Y., Sun Z.-W., Iratni R., Erdjument-Bromage H., Tempst P., Hampsey M., Reinberg D.
      Mol. Cell 1:1021-1031(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH HDAC1.
      Tissue: Cervix carcinoma.
    2. "SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors."
      Laherty C.D., Billin A.N., Lavinsky R.M., Yochum G.S., Bush A.C., Sun J.-M., Mullen T.-M., Davie J.R., Rose D.W., Glass C.K., Rosenfeld M.G., Ayer D.E., Eisenman R.N.
      Mol. Cell 2:33-42(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: BrainImported.
    5. "Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1."
      Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W.
      Genes Dev. 17:896-911(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HCFC1.
    6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131; SER-138 AND THR-145, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Solution structure of a novel zinc finger motif in the SAP30 polypeptide of the Sin3 corepressor complex and its potential role in nucleic acid recognition."
      He Y., Imhoff R., Sahu A., Radhakrishnan I.
      Nucleic Acids Res. 37:2142-2152(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 64-131, DNA-BINDING, ZINC-FINGER, MUTAGENESIS OF CYS-67; CYS-68; HIS-108 AND CYS-112.

    Entry informationi

    Entry nameiSAP30_HUMAN
    AccessioniPrimary (citable) accession number: O75446
    Secondary accession number(s): D3DP38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3