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Protein

PERQ amino acid-rich with GYF domain-containing protein 1

Gene

GIGYF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1 (By similarity).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
PERQ amino acid-rich with GYF domain-containing protein 1
Alternative name(s):
GRB10-interacting GYF protein 1
Gene namesi
Name:GIGYF1
Synonyms:CDS2, PERQ1
ORF Names:PP3360
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:9126. GIGYF1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162389554.

Polymorphism and mutation databases

BioMutaiGIGYF1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035PERQ amino acid-rich with GYF domain-containing protein 1PRO_0000058314Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241PhosphoserineCombined sources
Modified residuei28 – 281PhosphoserineCombined sources
Modified residuei137 – 1371PhosphoserineCombined sources
Modified residuei230 – 2301PhosphoserineCombined sources
Modified residuei341 – 3411PhosphoserineBy similarity
Modified residuei638 – 6381PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75420.
MaxQBiO75420.
PaxDbiO75420.
PRIDEiO75420.

PTM databases

iPTMnetiO75420.
PhosphoSiteiO75420.

Expressioni

Gene expression databases

BgeeiO75420.
CleanExiHS_CDS2.
HS_GIGYF1.
GenevisibleiO75420. HS.

Organism-specific databases

HPAiHPA020999.
HPA023995.

Interactioni

Subunit structurei

Interacts with GRB10. This transient binding is increased under IGF1 stimulation and leads to recruitment of GIGYF1/GRB10 complex to IGF1 receptor (By similarity).By similarity

Protein-protein interaction databases

BioGridi122218. 16 interactions.
IntActiO75420. 14 interactions.
MINTiMINT-2875799.
STRINGi9606.ENSP00000275732.

Structurei

3D structure databases

ProteinModelPortaliO75420.
SMRiO75420. Positions 477-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini474 – 52249GYFPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi203 – 2108Poly-Glu
Compositional biasi266 – 2716Poly-Gly
Compositional biasi335 – 3395Poly-Glu
Compositional biasi548 – 61770Gln-richAdd
BLAST
Compositional biasi596 – 6027Poly-Pro
Compositional biasi603 – 6086Poly-Gln
Compositional biasi969 – 9757Poly-Gln

Domaini

The GYF domain interacts with GRB10.By similarity

Sequence similaritiesi

Belongs to the PERQ family.Curated
Contains 1 GYF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1862. Eukaryota.
ENOG4111AQ6. LUCA.
GeneTreeiENSGT00660000095550.
HOGENOMiHOG000290720.
HOVERGENiHBG082121.
InParanoidiO75420.
KOiK18730.
OMAiWDVHTSA.
OrthoDBiEOG7RNK1K.
PhylomeDBiO75420.
TreeFamiTF325513.

Family and domain databases

Gene3Di3.30.1490.40. 1 hit.
InterProiIPR003169. GYF.
[Graphical view]
PfamiPF02213. GYF. 1 hit.
[Graphical view]
SMARTiSM00444. GYF. 1 hit.
[Graphical view]
SUPFAMiSSF55277. SSF55277. 1 hit.
PROSITEiPS50829. GYF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAETLNFGP EWLRALSGGG SVASPPPSPA MPKYKLADYR YGREEMLALY
60 70 80 90 100
VKENKVPEEL QDKEFAAVLQ DEPLQPLALE PLTEEEQRNF SLSVNSVAVL
110 120 130 140 150
RLMGKGAGPP LAGTSRGRGS TRSRGRGRGD SCFYQRSIEE GDGAFGRSPR
160 170 180 190 200
EIQRSQSWDD RGERRFEKSA RRDGARCGFE EGGAGPRKEH ARSDSENWRS
210 220 230 240 250
LREEQEEEEE GSWRLGAGPR RDGDRWRSAS PDGGPRSAGW REHGERRRKF
260 270 280 290 300
EFDLRGDRGG CGEEEGRGGG GSSHLRRCRA PEGFEEDKDG LPEWCLDDED
310 320 330 340 350
EEMGTFDASG AFLPLKKGPK EPIPEEQELD FQGLEEEEEP SEGLEEEGPE
360 370 380 390 400
AGGKELTPLP PQEEKSSSPS PLPTLGPLWG TNGDGDETAE KEPPAAEDDI
410 420 430 440 450
RGIQLSPGVG SSAGPPGDLE DDEGLKHLQQ EAEKLVASLQ DSSLEEEQFT
460 470 480 490 500
AAMQTQGLRH SAAATALPLS HGAARKWFYK DPQGEIQGPF TTQEMAEWFQ
510 520 530 540 550
AGYFSMSLLV KRGCDEGFQP LGEVIKMWGR VPFAPGPSPP PLLGNMDQER
560 570 580 590 600
LKKQQELAAA ALYQQLQHQQ FLQLVSSRQL PQCALREKAA LGDLTPPPPP
610 620 630 640 650
PPQQQQQQLT AFLQQLQALK PPRGGDQNLL PTMSRSLSVP DSGRLWDVHT
660 670 680 690 700
SASSQSGGEA SLWDIPINSS TQGPILEQLQ LQHKFQERRE VELRAKREEE
710 720 730 740 750
ERKRREEKRR QQQQEEQKRR QEEEELFRRK HVRQQELLLK LLQQQQAVPV
760 770 780 790 800
PPAPSSPPPL WAGLAKQGLS MKTLLELQLE GERQLHKQPP PREPARAQAP
810 820 830 840 850
NHRVQLGGLG TAPLNQWVSE AGPLWGGPDK SGGGSSGLGL WEDTPKSGGS
860 870 880 890 900
LVRGLGLKNS RSSPSLSDSY SHLSGRPIRK KTEEEEKLLK LLQGIPRPQD
910 920 930 940 950
GFTQWCEQML HTLSATGSLD VPMAVAILKE VESPYDVHDY IRSCLGDTLE
960 970 980 990 1000
AKEFAKQFLE RRAKQKASQQ RQQQQEAWLS SASLQTAFQA NHSTKLGPGE
1010 1020 1030
GSKAKRRALM LHSDPSILGY SLHGSSGEIE SVDDY
Length:1,035
Mass (Da):114,601
Last modified:December 12, 2006 - v2
Checksum:i7DACB6F80EBEA7D0
GO

Sequence cautioni

The sequence AAC78792.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAL55738.1 differs from that shown. Reason: Frameshift at positions 784 and 856. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti729 – 7302RK → DE in AAL55738 (PubMed:15498874).Curated
Sequence conflicti835 – 8351S → T in AAL55738 (PubMed:15498874).Curated
Sequence conflicti851 – 8511L → V in AAL55738 (PubMed:15498874).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053356 Genomic DNA. Translation: AAC78792.1. Sequence problems.
AY176044 mRNA. Translation: AAO46888.1.
AF289554 mRNA. Translation: AAL55738.1. Frameshift.
CCDSiCCDS34708.1.
RefSeqiNP_072096.2. NM_022574.4.
XP_005250589.1. XM_005250532.1.
UniGeneiHs.414396.

Genome annotation databases

EnsembliENST00000275732; ENSP00000275732; ENSG00000146830.
GeneIDi64599.
KEGGihsa:64599.
UCSCiuc003uwg.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053356 Genomic DNA. Translation: AAC78792.1. Sequence problems.
AY176044 mRNA. Translation: AAO46888.1.
AF289554 mRNA. Translation: AAL55738.1. Frameshift.
CCDSiCCDS34708.1.
RefSeqiNP_072096.2. NM_022574.4.
XP_005250589.1. XM_005250532.1.
UniGeneiHs.414396.

3D structure databases

ProteinModelPortaliO75420.
SMRiO75420. Positions 477-525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122218. 16 interactions.
IntActiO75420. 14 interactions.
MINTiMINT-2875799.
STRINGi9606.ENSP00000275732.

PTM databases

iPTMnetiO75420.
PhosphoSiteiO75420.

Polymorphism and mutation databases

BioMutaiGIGYF1.

Proteomic databases

EPDiO75420.
MaxQBiO75420.
PaxDbiO75420.
PRIDEiO75420.

Protocols and materials databases

DNASUi64599.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000275732; ENSP00000275732; ENSG00000146830.
GeneIDi64599.
KEGGihsa:64599.
UCSCiuc003uwg.2. human.

Organism-specific databases

CTDi64599.
GeneCardsiGIGYF1.
HGNCiHGNC:9126. GIGYF1.
HPAiHPA020999.
HPA023995.
MIMi612064. gene.
neXtProtiNX_O75420.
PharmGKBiPA162389554.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1862. Eukaryota.
ENOG4111AQ6. LUCA.
GeneTreeiENSGT00660000095550.
HOGENOMiHOG000290720.
HOVERGENiHBG082121.
InParanoidiO75420.
KOiK18730.
OMAiWDVHTSA.
OrthoDBiEOG7RNK1K.
PhylomeDBiO75420.
TreeFamiTF325513.

Miscellaneous databases

ChiTaRSiGIGYF1. human.
GenomeRNAii64599.
NextBioi66561.
PROiO75420.
SOURCEiSearch...

Gene expression databases

BgeeiO75420.
CleanExiHS_CDS2.
HS_GIGYF1.
GenevisibleiO75420. HS.

Family and domain databases

Gene3Di3.30.1490.40. 1 hit.
InterProiIPR003169. GYF.
[Graphical view]
PfamiPF02213. GYF. 1 hit.
[Graphical view]
SMARTiSM00444. GYF. 1 hit.
[Graphical view]
SUPFAMiSSF55277. SSF55277. 1 hit.
PROSITEiPS50829. GYF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes."
    Gloeckner G., Scherer S., Schattevoy R., Boright A.P., Weber J., Tsui L.-C., Rosenthal A.
    Genome Res. 8:1060-1073(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two novel proteins that are linked to insulin-like growth factor (IGF-I) receptors by the Grb10 adapter and modulate IGF-I signaling."
    Giovannone B., Lee E., Laviola L., Giorgino F., Cleveland K.A., Smith R.J.
    J. Biol. Chem. 278:31564-31573(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Placenta.
  3. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 729-1035.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24 AND SER-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPERQ1_HUMAN
AccessioniPrimary (citable) accession number: O75420
Secondary accession number(s): Q6Y7W7, Q8WZ38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: December 12, 2006
Last modified: May 11, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.