Reviewed, UniProtKB/Swiss-Prot O75419 (CC45L_HUMAN)

Names and origin

Protein names

Recommended name:
    CDC45-related protein
      Short name=Cdc45
Alternative name(s):
    PORC-PI-1

Gene names

Name:	CDC45L
Synonyms:	CDC45L2
ORF Names:	UNQ374/PRO710

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for initiation of chromosomal DNA replication.
Subunit structure	Associated with ORC2L.
Subcellular location	Cytoplasm. Nucleus.
Tissue specificity	Widely expressed, highest levels are found in adult testis and tyhmus and in fetal liver.
Developmental stage	Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
Sequence similarities	Belongs to the CDC45 family.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	DNA replication checkpoint Ref.2 Traceable author statement. Source: ProtInc DNA replication initiation Ref.2 Traceable author statement. Source: ProtInc cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from direct assay. Source: HPA nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDKN1A	P38936	1	EBI-374969,EBI-375077
CDKN2A	P42771	1	EBI-374969,EBI-375053
MCM10	Q7L590	1	EBI-374969,EBI-374912
ORC3L	Q9UBD5	1	EBI-374969,EBI-374916
ORC5L	O43913	1	EBI-374969,EBI-374928
TOPBP1	Q92547	2	EBI-374969,EBI-308302

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	CDC45-related protein		PRO_0000192815
Amino acid modifications
	Modified residue	130	1	Phosphotyrosine Ref.9
	Modified residue	144	1	Phosphoserine Ref.9
	Modified residue	148	1	Phosphoserine Ref.9
	Modified residue	151	1	Phosphoserine Ref.9
Natural variations
	Natural variant	81	1	V → I: dbSNP rs13447203. Ref.7		VAR_019286
	Natural variant	356	1	M → R: dbSNP rs17209274.		VAR_053026
	Natural variant	376	1	V → M: dbSNP rs13447263. Ref.7		VAR_019287
Experimental info
	Sequence conflict	100	1	T → S in AAQ89330. Ref.5
	Sequence conflict	115	1	I → V in CAA11530. Ref.3
	Sequence conflict	346	1	E → Q in AAC67521. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O75419-1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: AE1BE2C8C8E5F867	FASTA	566	65,569
	10 20 30 40 50 60 MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET 70 80 90 100 110 120 AFLEHKEQFH YFILINCGAN VDLLDILQPD EDTIFFVCDT HRPVNVVNVY NDTQIKLLIK 130 140 150 160 170 180 QDDDLEVPAY EDIFRDEEED EEHSGNDSDG SEPSEKRTRL EEEIVEQTMR RRQRREWEAR 190 200 210 220 230 240 RRDILFDYEQ YEYHGTSSAM VMFELAWMLS KDLNDMLWWA IVGLTDQWVQ DKITQMKYVT 250 260 270 280 290 300 DVGVLQRHVS RHNHRNEDEE NTLSVDCTRI SFEYDLRLVL YQHWSLHDSL CNTSYTAARF 310 320 330 340 350 360 KLWSVHGQKR LQEFLADMGL PLKQVKQKFQ AMDISLKENL REMIEESANK FGMKDMRVQT 370 380 390 400 410 420 FSIHFGFKHK FLASDVVFAT MSLMESPEKD GSGTDHFIQA LDSLSRSNLD KLYHGLELAK 430 440 450 460 470 480 KQLRATQQTI ASCLCTNLVI SQGPFLYCSL MEGTPDVMLF SRPASLSLLS KHLLKSFVCS 490 500 510 520 530 540 TKNRRCKLLP LVMAAPLSME HGTVTVVGIP PETDSSDRKN FFGRAFEKAA ESTSSRMLHN 550 560 HFDLSVIELK AEDRSKFLDA LISLLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk." Mimura S., Takisawa H. EMBO J. 17:5699-5707(1998) [PubMed: 9755170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The human homolog of Saccharomyces cerevisiae CDC45." Saha P., Thome K.C., Yamaguchi R., Hou Z.-H., Weremowicz S., Dutta A. J. Biol. Chem. 273:18205-18209(1998) [PubMed: 9660782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Direct selection of conserved cDNAs from the DiGeorge critical region: isolation of a novel CDC45-like gene." McKie J.M., Wadey R.B., Sutherland H.F., Taylor C.L., Scambler P.J. Genome Res. 8:834-841(1998) [PubMed: 9724329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Characterization of CDC45L: a gene in the 22q11.2 deletion region expressed during murine and human development." Shaikh T.H., Gottlieb S., Sellinger B., Chen F., Roe B.A., Oakey R.J., Emanuel B.S., Budarf M.L. Mamm. Genome 10:322-326(1999) [PubMed: 10051334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	NIEHS SNPs program Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-81 AND MET-376.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Muscle.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-130; SER-144; SER-148 AND SER-151, MASS SPECTROMETRY.
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF062495 mRNA. Translation: AAC67521.1. AF053074 mRNA. Translation: AAC27289.1. AJ223728 mRNA. Translation: CAA11530.1. AF081535 mRNA. Translation: AAD08998.1. AY358971 mRNA. Translation: AAQ89330.1. BT006792 mRNA. Translation: AAP35438.1. AY572790 Genomic DNA. Translation: AAS66985.1. BC006232 mRNA. Translation: AAH06232.1. BC010022 mRNA. Translation: AAH10022.1.
IPI	IPI00025695.
RefSeq	NP_003495.1.
UniGene	Hs.474217
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O75419. 10 interactions.
PTM databases
PhosphoSite	O75419.
Proteomic databases
PeptideAtlas	O75419.
PRIDE	O75419.
Genome annotation databases
Ensembl	ENSG00000093009. Homo sapiens. [Contig view]
GeneID	8318.
KEGG	hsa:8318.
Organism-specific databases
GeneCards	GC22P017841.
H-InvDB	HIX0016238.
HGNC	HGNC:1739. CDC45L.
HPA	CAB009459. HPA000614.
MIM	603465. gene.
PharmGKB	PA371.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O75419.
HOVERGEN	O75419.
OMA	O75419. FKHKFLA.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	O75419.
Bgee	O75419.
CleanEx	HS_CDC45L.
GermOnline	ENSG00000093009. Homo sapiens.
Family and domain databases
InterPro	IPR003874. CDC45-like. [Graphical view]
PANTHER	PTHR10507. CDC45_like. 1 hit.
Pfam	PF02724. CDC45. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	31151.
SOURCE	Search...

Entry information

Entry name

CC45L_HUMAN

Accession

Primary (citable) accession number: O75419
Secondary accession number(s): O60856, Q6UW54, Q9UP68

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1998
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)