O75414 (NDK6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 6
Short name=NDK 6
Short name=NDP kinase 6
EC=2.7.4.6

Alternative name(s):
Inhibitor of p53-induced apoptosis-alpha
Short name=IPIA-alpha
nm23-H6

Gene names

Name:

NME6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Inhibitor of p53-induced apoptosis.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Tissue specificity	Expressed at a moderately low level in many tissues. Most abundant in kidney, prostate, ovary, intestine, and spleen.
Sequence similarities	Belongs to the NDK family.
Sequence caution	The sequence AAC69439.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH01808.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH12828.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD96275.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis Nucleotide metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Non-traceable author statement. Source: UniProtKB negative regulation of cell growth Inferred from direct assay PubMed 10618642. Source: UniProtKB negative regulation of mitosis Inferred from direct assay PubMed 10618642. Source: UniProtKB nucleoside triphosphate biosynthetic process Non-traceable author statement. Source: UniProtKB
Cellular_component	mitochondrion Inferred from direct assay PubMed 10618642. Source: UniProtKB
Molecular_function	ATP binding Non-traceable author statement. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from direct assay PubMed 10618642. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75414-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75414-2)

The sequence of this isoform differs from the canonical sequence as follows:
     65-131: Missing.

Isoform 3 (identifier: O75414-3)

The sequence of this isoform differs from the canonical sequence as follows:
     65-186: GRFFYQRLVE...VAGTGGLGPA → AGQSEPTSLP...AIAQREVSTM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Nucleoside diphosphate kinase 6		PRO_0000137127
Sites
	Active site	129	1	Pros-phosphohistidine intermediate By similarity
	Binding site	19	1	ATP By similarity
	Binding site	68	1	ATP By similarity
	Binding site	96	1	ATP By similarity
	Binding site	102	1	ATP By similarity
	Binding site	116	1	ATP By similarity
	Binding site	126	1	ATP By similarity
Natural variations
	Alternative sequence	65 – 186	122	GRFFY…GLGPA → AGQSEPTSLPTRMPSSSGGR SWDPPECSEHAMWPQILSVG VSASLTPATPPMVRTLWFQP AERLQPSSLTSVNSAGMRRK SPSCAVALCAIAQREVSTM in isoform 3.		VSP_036883
	Alternative sequence	65 – 131	67	Missing in isoform 2.		VSP_036882
Experimental info
	Sequence conflict	182	1	G → S in BAD96275. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2001. Version 3. Checksum: DB8C5E046FACD143	FASTA	186	21,142
	10 20 30 40 50 60 MASILRSPQA LQLTLALIKP DAVAHPLILE AVHQQILSNK FLIVRMRELL WRKEDCQRFY 70 80 90 100 110 120 REHEGRFFYQ RLVEFMASGP IRAYILAHKD AIQLWRTLMG PTRVFRARHV APDSIRGSFG 130 140 150 160 170 180 LTDTRNTTHG SDSVVSASRE IAAFFPDFSE QRWYEEEEPQ LRCGPVCYSP EGGVHYVAGT GGLGPA
	Isoform 2 [UniParc]. Checksum: 57FB2BF44A5619F5	FASTA	119	13,510
	10 20 30 40 50 60 MASILRSPQA LQLTLALIKP DAVAHPLILE AVHQQILSNK FLIVRMRELL WRKEDCQRFY 70 80 90 100 110 REHEDSVVSA SREIAAFFPD FSEQRWYEEE EPQLRCGPVC YSPEGGVHYV AGTGGLGPA
	Isoform 3 [UniParc]. Checksum: A27E767C901CC647	FASTA	163	18,087
	10 20 30 40 50 60 MASILRSPQA LQLTLALIKP DAVAHPLILE AVHQQILSNK FLIVRMRELL WRKEDCQRFY 70 80 90 100 110 120 REHEAGQSEP TSLPTRMPSS SGGRSWDPPE CSEHAMWPQI LSVGVSASLT PATPPMVRTL 130 140 150 160 WFQPAERLQP SSLTSVNSAG MRRKSPSCAV ALCAIAQREV STM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NME6: a new member of the nm23/nucleoside diphosphate kinase gene family located on human chromosome 3p21.3." Mehus J.G., Deloukas P., Lambeth D.O. Hum. Genet. 104:454-459(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[2]	"Identification of a gene that inhibits p53-induced apoptosis." Nakamura H., Tsuiki H., Honda Y., Sasaki J., Masuko N., Akagi K., Saya H. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Brain.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Coronary artery.
[5]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF051941 mRNA. Translation: AAC78463.1. U90449 mRNA. Translation: AAC69439.1. Different initiation. AK294809 mRNA. Translation: BAG57928.1. AK297364 mRNA. Translation: BAG59811.1. AK222555 mRNA. Translation: BAD96275.1. Different initiation. AC105267 Genomic DNA. No translation available. BC001808 mRNA. Translation: AAH01808.1. Different initiation. BC012828 mRNA. Translation: AAH12828.1. Different initiation.
IPI	IPI00218214. IPI00910570. IPI00910598.
RefSeq	NP_005784.1. NM_005793.3.
UniGene	Hs.465558.
3D structure databases
ProteinModelPortal	O75414.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75414. 2 interactions.
STRING	9606.ENSP00000416658.
Proteomic databases
PaxDb	O75414.
PRIDE	O75414.
Protocols and materials databases
DNASU	10201.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000415053; ENSP00000399582; ENSG00000172113. ENST00000415644; ENSP00000394232; ENSG00000172113. ENST00000421967; ENSP00000416658; ENSG00000172113. ENST00000426689; ENSP00000440286; ENSG00000172113. ENST00000426723; ENSP00000391499; ENSG00000172113. ENST00000435684; ENSP00000393261; ENSG00000172113. ENST00000442597; ENSP00000406642; ENSG00000172113. ENST00000450160; ENSP00000398070; ENSG00000172113. ENST00000451657; ENSP00000407933; ENSG00000172113. ENST00000452211; ENSP00000392352; ENSG00000172113.
GeneID	10201.
KEGG	hsa:10201.
UCSC	uc003cso.3. human. uc011bbh.2. human. uc011bbi.2. human.
Organism-specific databases
CTD	10201.
GeneCards	GC03M048334.
H-InvDB	HIX0019874.
HGNC	HGNC:20567. NME6.
HPA	HPA017909.
MIM	608294. gene.
neXtProt	NX_O75414.
PharmGKB	PA134873104.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0105.
HOGENOM	HOG000224565.
HOVERGEN	HBG001619.
InParanoid	O75414.
KO	K00940.
OrthoDB	EOG4SQWXQ.
Gene expression databases
ArrayExpress	O75414.
Bgee	O75414.
CleanEx	HS_NME6.
Genevestigator	O75414.
GermOnline	ENSG00000172113. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.141. 1 hit.
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
PANTHER	PTHR11349. PTHR11349. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4480.
GenomeRNAi	10201.
NextBio	38610.
SOURCE	Search...

Entry information

Entry name

NDK6_HUMAN

Accession

Primary (citable) accession number: O75414
Secondary accession number(s): B4DGW7 , B4DM99, Q53HM5, Q96E73, Q9BQ63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.