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Protein

Nucleoside diphosphate kinase 6

Gene

NME6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Inhibitor of p53-induced apoptosis.

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191ATPBy similarity
Binding sitei68 – 681ATPBy similarity
Binding sitei96 – 961ATPBy similarity
Binding sitei102 – 1021ATPBy similarity
Binding sitei116 – 1161ATPBy similarity
Binding sitei126 – 1261ATPBy similarity
Active sitei129 – 1291Pros-phosphohistidine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleoside diphosphate kinase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. CTP biosynthetic process Source: InterPro
  3. GTP biosynthetic process Source: InterPro
  4. negative regulation of cell growth Source: UniProtKB
  5. negative regulation of mitotic nuclear division Source: UniProtKB
  6. UTP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Apoptosis, Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase 6 (EC:2.7.4.6)
Short name:
NDK 6
Short name:
NDP kinase 6
Alternative name(s):
Inhibitor of p53-induced apoptosis-alpha
Short name:
IPIA-alpha
nm23-H6
Gene namesi
Name:NME6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:20567. NME6.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134873104.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186Nucleoside diphosphate kinase 6PRO_0000137127Add
BLAST

Proteomic databases

MaxQBiO75414.
PaxDbiO75414.
PRIDEiO75414.

Expressioni

Tissue specificityi

Expressed at a moderately low level in many tissues. Most abundant in kidney, prostate, ovary, intestine, and spleen.

Gene expression databases

BgeeiO75414.
CleanExiHS_NME6.
ExpressionAtlasiO75414. baseline and differential.
GenevestigatoriO75414.

Organism-specific databases

HPAiHPA017909.

Interactioni

Protein-protein interaction databases

BioGridi115496. 3 interactions.
IntActiO75414. 2 interactions.
MINTiMINT-4656535.
STRINGi9606.ENSP00000416658.

Structurei

3D structure databases

ProteinModelPortaliO75414.
SMRiO75414. Positions 14-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiCOG0105.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224565.
HOVERGENiHBG001619.
InParanoidiO75414.
KOiK00940.
OrthoDBiEOG7QZGCP.
PhylomeDBiO75414.
TreeFamiTF354225.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75414-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASILRSPQA LQLTLALIKP DAVAHPLILE AVHQQILSNK FLIVRMRELL
60 70 80 90 100
WRKEDCQRFY REHEGRFFYQ RLVEFMASGP IRAYILAHKD AIQLWRTLMG
110 120 130 140 150
PTRVFRARHV APDSIRGSFG LTDTRNTTHG SDSVVSASRE IAAFFPDFSE
160 170 180
QRWYEEEEPQ LRCGPVCYSP EGGVHYVAGT GGLGPA
Length:186
Mass (Da):21,142
Last modified:January 10, 2001 - v3
Checksum:iDB8C5E046FACD143
GO
Isoform 2 (identifier: O75414-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-131: Missing.

Show »
Length:119
Mass (Da):13,510
Checksum:i57FB2BF44A5619F5
GO
Isoform 3 (identifier: O75414-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     65-186: GRFFYQRLVE...VAGTGGLGPA → AGQSEPTSLP...AIAQREVSTM

Show »
Length:163
Mass (Da):18,087
Checksum:iA27E767C901CC647
GO

Sequence cautioni

The sequence AAC69439.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH01808.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH12828.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAD96275.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821G → S in BAD96275 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei65 – 186122GRFFY…GLGPA → AGQSEPTSLPTRMPSSSGGR SWDPPECSEHAMWPQILSVG VSASLTPATPPMVRTLWFQP AERLQPSSLTSVNSAGMRRK SPSCAVALCAIAQREVSTM in isoform 3. 1 PublicationVSP_036883Add
BLAST
Alternative sequencei65 – 13167Missing in isoform 2. 1 PublicationVSP_036882Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051941 mRNA. Translation: AAC78463.1.
U90449 mRNA. Translation: AAC69439.1. Different initiation.
AK294809 mRNA. Translation: BAG57928.1.
AK297364 mRNA. Translation: BAG59811.1.
AK222555 mRNA. Translation: BAD96275.1. Different initiation.
AC105267 Genomic DNA. No translation available.
BC001808 mRNA. Translation: AAH01808.1. Different initiation.
BC012828 mRNA. Translation: AAH12828.1. Different initiation.
RefSeqiNP_005784.1. NM_005793.3.
XP_005264849.1. XM_005264792.1. [O75414-1]
XP_005264851.1. XM_005264794.2. [O75414-3]
UniGeneiHs.465558.

Genome annotation databases

EnsembliENST00000415053; ENSP00000399582; ENSG00000172113. [O75414-1]
ENST00000415644; ENSP00000394232; ENSG00000172113. [O75414-2]
ENST00000426689; ENSP00000440286; ENSG00000172113. [O75414-1]
ENST00000435684; ENSP00000393261; ENSG00000172113. [O75414-3]
ENST00000442597; ENSP00000406642; ENSG00000172113. [O75414-1]
ENST00000451657; ENSP00000407933; ENSG00000172113. [O75414-3]
ENST00000452211; ENSP00000392352; ENSG00000172113. [O75414-1]
GeneIDi10201.
KEGGihsa:10201.
UCSCiuc003cso.3. human. [O75414-1]
uc010hju.4. human. [O75414-3]
uc011bbi.2. human. [O75414-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051941 mRNA. Translation: AAC78463.1.
U90449 mRNA. Translation: AAC69439.1. Different initiation.
AK294809 mRNA. Translation: BAG57928.1.
AK297364 mRNA. Translation: BAG59811.1.
AK222555 mRNA. Translation: BAD96275.1. Different initiation.
AC105267 Genomic DNA. No translation available.
BC001808 mRNA. Translation: AAH01808.1. Different initiation.
BC012828 mRNA. Translation: AAH12828.1. Different initiation.
RefSeqiNP_005784.1. NM_005793.3.
XP_005264849.1. XM_005264792.1. [O75414-1]
XP_005264851.1. XM_005264794.2. [O75414-3]
UniGeneiHs.465558.

3D structure databases

ProteinModelPortaliO75414.
SMRiO75414. Positions 14-146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115496. 3 interactions.
IntActiO75414. 2 interactions.
MINTiMINT-4656535.
STRINGi9606.ENSP00000416658.

Proteomic databases

MaxQBiO75414.
PaxDbiO75414.
PRIDEiO75414.

Protocols and materials databases

DNASUi10201.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000415053; ENSP00000399582; ENSG00000172113. [O75414-1]
ENST00000415644; ENSP00000394232; ENSG00000172113. [O75414-2]
ENST00000426689; ENSP00000440286; ENSG00000172113. [O75414-1]
ENST00000435684; ENSP00000393261; ENSG00000172113. [O75414-3]
ENST00000442597; ENSP00000406642; ENSG00000172113. [O75414-1]
ENST00000451657; ENSP00000407933; ENSG00000172113. [O75414-3]
ENST00000452211; ENSP00000392352; ENSG00000172113. [O75414-1]
GeneIDi10201.
KEGGihsa:10201.
UCSCiuc003cso.3. human. [O75414-1]
uc010hju.4. human. [O75414-3]
uc011bbi.2. human. [O75414-2]

Organism-specific databases

CTDi10201.
GeneCardsiGC03M048334.
H-InvDBHIX0019874.
HGNCiHGNC:20567. NME6.
HPAiHPA017909.
MIMi608294. gene.
neXtProtiNX_O75414.
PharmGKBiPA134873104.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0105.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224565.
HOVERGENiHBG001619.
InParanoidiO75414.
KOiK00940.
OrthoDBiEOG7QZGCP.
PhylomeDBiO75414.
TreeFamiTF354225.

Miscellaneous databases

GenomeRNAii10201.
NextBioi38610.
PROiO75414.
SOURCEiSearch...

Gene expression databases

BgeeiO75414.
CleanExiHS_NME6.
ExpressionAtlasiO75414. baseline and differential.
GenevestigatoriO75414.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NME6: a new member of the nm23/nucleoside diphosphate kinase gene family located on human chromosome 3p21.3."
    Mehus J.G., Deloukas P., Lambeth D.O.
    Hum. Genet. 104:454-459(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  2. "Identification of a gene that inhibits p53-induced apoptosis."
    Nakamura H., Tsuiki H., Honda Y., Sasaki J., Masuko N., Akagi K., Saya H.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Brain.
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Coronary artery.
  5. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNDK6_HUMAN
AccessioniPrimary (citable) accession number: O75414
Secondary accession number(s): B4DGW7
, B4DM99, Q53HM5, Q96E73, Q9BQ63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2001
Last sequence update: January 10, 2001
Last modified: March 31, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.