O75396 (SC22B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-trafficking protein SEC22b

Alternative name(s):
ER-Golgi SNARE of 24 kDa
Short name=ERS-24
Short name=ERS24
SEC22 vesicle-trafficking protein homolog B
SEC22 vesicle-trafficking protein-like 1

Gene names

Name:	SEC22B
Synonyms:	SEC22L1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER. Ref.6
Subunit structure	Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex.
Subcellular location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein By similarity. Golgi apparatus membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.7
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.8 Ref.9 Ref.10 Ref.12
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.
Caution	The reference genome displays a polymorphic premature stop codon in position 39.
Sequence caution	The sequence AL359758 differs from that shown. Reason: Erroneous termination at position 39. Translated as Gln.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Traceable author statement. Source: ProtInc protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum-Golgi intermediate compartment membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 215

214

Vesicle-trafficking protein SEC22b

PRO_0000206770

Regions

Topological domain

2 – 194

193

Cytoplasmic Potential

Transmembrane

195 – 215

21

Helical; Anchor for type IV membrane protein; Potential

Domain

6 – 119

114

Longin

Domain

134 – 194

61

v-SNARE coiled-coil homology

Amino acid modifications

Modified residue

35

1

Phosphoserine Ref.8

Modified residue

38

1

N6-acetyllysine Ref.11

Modified residue

48

1

Phosphoserine Ref.9

Modified residue

137

1

Phosphoserine Ref.9 Ref.10 Ref.12

Modified residue

168

1

Phosphoserine Ref.12

Natural variations

Natural variant

71

1

D → Y. Ref.1 Ref.2 Ref.4
Corresponds to variant rs2596331 [ dbSNP | Ensembl ].

VAR_060311

Natural variant

82

1

T → K. Ref.1 Ref.2 Ref.4
Corresponds to variant rs2794053 [ dbSNP | Ensembl ].

VAR_060312

Natural variant

108

1

R → Q.
Corresponds to variant rs2655551 [ dbSNP | Ensembl ].

VAR_057343

Natural variant

130

1

C → R. Ref.1 Ref.2 Ref.4
Corresponds to variant rs2590131 [ dbSNP | Ensembl ].

VAR_060313

Natural variant

190

1

H → R. Ref.1 Ref.2 Ref.4
Corresponds to variant rs2655557 [ dbSNP | Ensembl ].

VAR_060314

Natural variant

214

1

W → C.
Corresponds to variant rs7534444 [ dbSNP | Ensembl ].

VAR_057344

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

215

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Turn

10 – 12

3

Beta strand

15 – 19

5

Helix

30 – 43

14

Beta strand

50 – 56

7

Beta strand

59 – 66

8

Beta strand

69 – 76

8

Helix

81 – 99

19

Turn

100 – 105

6

Turn

109 – 112

4

Helix

113 – 115

3

Helix

116 – 123

8

Turn

124 – 126

3

Beta strand

150 – 152

3

Helix

153 – 156

4

Sequences

	Sequence		Length	Mass (Da)
	O75396 [UniParc]. Last modified January 11, 2011. Version 4. Checksum: 9FA59C20F2D3F690	FASTA	215	24,593
	10 20 30 40 50 60 MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT RCTLEAGAMT 70 80 90 100 110 120 FHYIIEQGVC DLVLCEAAFP KTLAFAYLED LHSEFDEQHG KKVPTVSRPY SFIEFDTFIQ 130 140 150 160 170 180 KTKKLYIDSC ARRNLGSINT ELQDVQRIMV ANIEEVLQRG EALSALDSKA NNLSSLSKKY 190 200 210 RQDAKYLNMH STYAKLAAVA VFFIMLIVYV RFWWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed: 9653160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS TYR-71; LYS-82; ARG-130 AND ARG-190. Tissue: Umbilical cord blood.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS TYR-71; LYS-82; ARG-130 AND ARG-190. Tissue: Caudate nucleus.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS TYR-71; LYS-82; ARG-130 AND ARG-190. Tissue: Skin.
[5]	Bienvenut W.V., Claeys D. Submitted (NOV-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-9; 29-38 AND 134-147, CLEAVAGE OF INITIATOR METHIONINE, MASS SPECTROMETRY. Tissue: Platelet.
[6]	"Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K., Nagahama M., Tani K., Yamamoto A., Tagaya M. EMBO J. 23:3216-3226(2004) [PubMed: 15272311] [Abstract] Cited for: FUNCTION, INTERACTION WITH BNIP1; STX18 AND USE1L.
[7]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48 AND SER-137, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-38, MASS SPECTROMETRY.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137 AND SER-168, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF047442 mRNA. Translation: AAC39893.1.
AK289875 mRNA. Translation: BAF82564.1.
AL359758 Genomic DNA. No translation available.
BX537145 Genomic DNA. No translation available.
BC001364 mRNA. Translation: AAH01364.1.

IPI

IPI00006865.

RefSeq

NP_004883.2. NM_004892.4.

UniGene

Hs.632438.
Hs.715002.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NUP	X-ray	2.80	C	1-195	[»]
2NUT	X-ray	2.30	C	1-195	[»]
3EGD	X-ray	2.70	C	1-157	[»]
3EGX	X-ray	3.30	C	1-157	[»]

ProteinModelPortal

O75396.

SMR

O75396. Positions 1-186.

ModBase

Search...

Protein-protein interaction databases

IntAct

O75396. 4 interactions.

STRING

O75396.

PTM databases

PhosphoSite

O75396.

Proteomic databases

PeptideAtlas

O75396.

PRIDE

O75396.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

9554.

KEGG

hsa:9554.

Organism-specific databases

CTD

9554.

GeneCards

GC01P145096.

H-InvDB

HIX0023504.

HGNC

HGNC:10700. SEC22B.

MIM

604029. gene.

neXtProt

NX_O75396.

PharmGKB

PA35623.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

HBG327843.

HOVERGEN

HBG052748.

OrthoDB

EOG4XPQGX.

PhylomeDB

O75396.

Gene expression databases

ArrayExpress

O75396.

CleanEx

HS_SEC22B.

Genevestigator

O75396.

Family and domain databases

InterPro

IPR010908. Longin.
IPR011012. Longin-like.
IPR001388. Synaptobrevin.
[Graphical view]

Gene3D

G3DSA:3.30.450.50. Longin. 1 hit.

KO

K08517.

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PRINTS

PR00219. SYNAPTOBREVN.

SUPFAM

SSF64356. Longin_like. 1 hit.

PROSITE

PS50859. LONGIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

35831.

SOURCE

Search...

Entry information

Entry name

SC22B_HUMAN

Accession

Primary (citable) accession number: O75396
Secondary accession number(s): A8K1G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	January 11, 2011
Last modified:	December 14, 2011
This is version 105 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.