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O75391 (SPAG7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sperm-associated antigen 7
Gene names
Name:SPAG7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Nucleus Potential.

Tissue specificity

Detected in fetal brain. Ref.3

Sequence similarities

Contains 1 R3H domain.

Sequence caution

The sequence AAC39888.1 differs from that shown. Reason: Frameshift at position 227.

Ontologies

Keywords
   Cellular componentNucleus
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 227226Sperm-associated antigen 7
PRO_0000072097

Regions

Domain46 – 10964R3H
Motif35 – 5117Nuclear localization signal Potential
Motif122 – 13918Nuclear localization signal Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.5 Ref.8 Ref.9
Modified residue1141Phosphoserine Ref.4
Modified residue1581Phosphoserine Ref.4 Ref.6

Secondary structure

............... 227
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O75391 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: 429566BE9B276EE4

FASTA22726,034
        10         20         30         40         50         60 
MADLLGSILS SMEKPPSLGD QETRRKAREQ AARLKKLQEQ EKQQKVEFRK RMEKEVSDFI 

        70         80         90        100        110        120 
QDSGQIKKKF QPMNKIERSI LHDVVEVAGL TSFSFGEDDD CRYVMIFKKE FAPSDEELDS 

       130        140        150        160        170        180 
YRRGEEWDPQ KAEEKRKLKE LAQRQEEEAA QQGPVVVSPA SDYKDKYSHL IGKGAAKDAA 

       190        200        210        220 
HMLQANKTYG CVPVANKRDT RSIEEAMNEI RAKKRLRQSG EELPPTS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.
Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Umbilical cord blood.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Uterus.
[3]"Cloning and characterization of a fox sperm protein FSA-1."
Beaton S., Cleary A., ten Have J., Bradley M.P.
Reprod. Fertil. Dev. 6:761-770(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Solution structure of the R3H domain of human sperm-associated antigen 7."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 44-124.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF047437 mRNA. Translation: AAC39888.1. Frameshift.
BC011934 mRNA. Translation: AAH11934.1.
CCDSCCDS42240.1.
RefSeqNP_004881.2. NM_004890.2.
UniGeneHs.90436.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CPMNMR-A44-124[»]
ProteinModelPortalO75391.
SMRO75391. Positions 44-124.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114924. 2 interactions.
IntActO75391. 1 interaction.
MINTMINT-1033754.
STRING9606.ENSP00000206020.

PTM databases

PhosphoSiteO75391.

Proteomic databases

MaxQBO75391.
PaxDbO75391.
PRIDEO75391.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000206020; ENSP00000206020; ENSG00000091640.
GeneID9552.
KEGGhsa:9552.
UCSCuc002gae.3. human.

Organism-specific databases

CTD9552.
GeneCardsGC17M004862.
HGNCHGNC:11216. SPAG7.
HPAHPA024032.
HPA052394.
MIM610056. gene.
neXtProtNX_O75391.
PharmGKBPA36052.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG75013.
HOGENOMHOG000234363.
HOVERGENHBG066267.
InParanoidO75391.
OMAEESRYVM.
PhylomeDBO75391.
TreeFamTF323631.

Gene expression databases

ArrayExpressO75391.
BgeeO75391.
CleanExHS_SPAG7.
GenevestigatorO75391.

Family and domain databases

Gene3D3.30.1370.50. 1 hit.
InterProIPR001374. R3H_ss-bd.
IPR017330. Sperm-assoc_antigen_PAG7.
[Graphical view]
PfamPF01424. R3H. 1 hit.
[Graphical view]
PIRSFPIRSF037943. Sperm-assoc_antigen_PAG7. 1 hit.
SMARTSM00393. R3H. 1 hit.
[Graphical view]
SUPFAMSSF82708. SSF82708. 1 hit.
PROSITEPS51061. R3H. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO75391.
GeneWikiSPAG7.
GenomeRNAi9552.
NextBio35821.
PROO75391.
SOURCESearch...

Entry information

Entry nameSPAG7_HUMAN
AccessionPrimary (citable) accession number: O75391
Secondary accession number(s): Q96EU5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM