O75391 (SPAG7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sperm-associated antigen 7

Gene names

Name:

SPAG7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus Potential.
Tissue specificity	Detected in fetal brain. Ref.3
Sequence similarities	Contains 1 R3H domain.
Sequence caution	The sequence AAC39888.1 differs from that shown. Reason: Frameshift at position 227.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 227

227

Sperm-associated antigen 7

PRO_0000072097

Regions

Domain

46 – 109

64

R3H

Motif

35 – 51

17

Nuclear localization signal Potential

Motif

122 – 139

18

Nuclear localization signal Potential

Amino acid modifications

Modified residue

114

1

Phosphoserine Ref.4

Modified residue

158

1

Phosphoserine Ref.4 Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

227

Helix Strand Turn

Details...

Helix

45 – 61

17

Beta strand

67 – 69

3

Helix

77 – 88

12

Beta strand

91 – 95

5

Beta strand

97 – 100

4

Beta strand

103 – 107

5

Helix

115 – 123

9

Sequences

	Sequence		Length	Mass (Da)
	O75391 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 429566BE9B276EE4	FASTA	227	26,034
	10 20 30 40 50 60 MADLLGSILS SMEKPPSLGD QETRRKAREQ AARLKKLQEQ EKQQKVEFRK RMEKEVSDFI 70 80 90 100 110 120 QDSGQIKKKF QPMNKIERSI LHDVVEVAGL TSFSFGEDDD CRYVMIFKKE FAPSDEELDS 130 140 150 160 170 180 YRRGEEWDPQ KAEEKRKLKE LAQRQEEEAA QQGPVVVSPA SDYKDKYSHL IGKGAAKDAA 190 200 210 220 HMLQANKTYG CVPVANKRDT RSIEEAMNEI RAKKRLRQSG EELPPTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	"Cloning and characterization of a fox sperm protein FSA-1." Beaton S., Cleary A., ten Have J., Bradley M.P. Reprod. Fertil. Dev. 6:761-770(1994) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-158, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Solution structure of the R3H domain of human sperm-associated antigen 7." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 44-124.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF047437 mRNA. Translation: AAC39888.1. Frameshift.
BC011934 mRNA. Translation: AAH11934.1.

IPI

IPI00006863.

RefSeq

NP_004881.2. NM_004890.2.

UniGene

Hs.90436.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CPM	NMR	-	A	44-124	[»]

ProteinModelPortal

O75391.

ModBase

Search...

Protein-protein interaction databases

IntAct

O75391. 1 interaction.

MINT

MINT-1033754.

STRING

9606.ENSP00000206020.

PTM databases

PhosphoSite

O75391.

Proteomic databases

PaxDb

O75391.

PRIDE

O75391.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000206020; ENSP00000206020; ENSG00000091640.

GeneID

9552.

KEGG

hsa:9552.

UCSC

uc002gae.3. human.

Organism-specific databases

CTD

9552.

GeneCards

GC17M004862.

HGNC

HGNC:11216. SPAG7.

HPA

HPA024032.

MIM

610056. gene.

neXtProt

NX_O75391.

PharmGKB

PA36052.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG75013.

HOGENOM

HOG000234363.

HOVERGEN

HBG066267.

InParanoid

O75391.

OMA

EESRYVM.

OrthoDB

EOG4GF3G4.

Gene expression databases

ArrayExpress

O75391.

Bgee

O75391.

CleanEx

HS_SPAG7.

Genevestigator

O75391.

GermOnline

ENSG00000091640. Homo sapiens.

Family and domain databases

InterPro

IPR001374. R3H_ss-bd.
IPR017330. Sperm-assoc_antigen_PAG7.
[Graphical view]

Pfam

PF01424. R3H. 1 hit.
[Graphical view]

PIRSF

PIRSF037943. Sperm-assoc_antigen_PAG7. 1 hit.

SMART

SM00393. R3H. 1 hit.
[Graphical view]

PROSITE

PS51061. R3H. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O75391.

GenomeRNAi

9552.

NextBio

35821.

SOURCE

Search...

Entry information

Entry name

SPAG7_HUMAN

Accession

Primary (citable) accession number: O75391
Secondary accession number(s): Q96EU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.