Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sperm-associated antigen 7

Gene

SPAG7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 7
Gene namesi
Name:SPAG7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:11216. SPAG7.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36052.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 227226Sperm-associated antigen 7PRO_0000072097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine3 Publications
Modified residuei114 – 1141Phosphoserine1 Publication
Modified residuei158 – 1581Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO75391.
PaxDbiO75391.
PRIDEiO75391.

PTM databases

PhosphoSiteiO75391.

Expressioni

Tissue specificityi

Detected in fetal brain.1 Publication

Gene expression databases

BgeeiO75391.
CleanExiHS_SPAG7.
ExpressionAtlasiO75391. baseline and differential.
GenevestigatoriO75391.

Organism-specific databases

HPAiHPA024032.
HPA052394.

Interactioni

Protein-protein interaction databases

BioGridi114924. 3 interactions.
IntActiO75391. 1 interaction.
MINTiMINT-1033754.
STRINGi9606.ENSP00000206020.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 6117Combined sources
Beta strandi67 – 693Combined sources
Helixi77 – 8812Combined sources
Beta strandi91 – 955Combined sources
Beta strandi97 – 1004Combined sources
Beta strandi103 – 1075Combined sources
Helixi115 – 1239Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CPMNMR-A44-124[»]
ProteinModelPortaliO75391.
SMRiO75391. Positions 44-124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75391.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 10964R3HPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 5117Nuclear localization signalSequence AnalysisAdd
BLAST
Motifi122 – 13918Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 R3H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG75013.
GeneTreeiENSGT00390000001520.
HOGENOMiHOG000234363.
HOVERGENiHBG066267.
InParanoidiO75391.
OMAiEESRYVM.
PhylomeDBiO75391.
TreeFamiTF323631.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
InterProiIPR001374. R3H_ss-bd.
IPR017330. Sperm-assoc_antigen_PAG7.
[Graphical view]
PfamiPF01424. R3H. 1 hit.
[Graphical view]
PIRSFiPIRSF037943. Sperm-assoc_antigen_PAG7. 1 hit.
SMARTiSM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O75391-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADLLGSILS SMEKPPSLGD QETRRKAREQ AARLKKLQEQ EKQQKVEFRK
60 70 80 90 100
RMEKEVSDFI QDSGQIKKKF QPMNKIERSI LHDVVEVAGL TSFSFGEDDD
110 120 130 140 150
CRYVMIFKKE FAPSDEELDS YRRGEEWDPQ KAEEKRKLKE LAQRQEEEAA
160 170 180 190 200
QQGPVVVSPA SDYKDKYSHL IGKGAAKDAA HMLQANKTYG CVPVANKRDT
210 220
RSIEEAMNEI RAKKRLRQSG EELPPTS
Length:227
Mass (Da):26,034
Last modified:March 29, 2005 - v2
Checksum:i429566BE9B276EE4
GO

Sequence cautioni

The sequence AAC39888.1 differs from that shown. Reason: Frameshift at position 227. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047437 mRNA. Translation: AAC39888.1. Frameshift.
BC011934 mRNA. Translation: AAH11934.1.
CCDSiCCDS42240.1.
RefSeqiNP_004881.2. NM_004890.2.
UniGeneiHs.90436.

Genome annotation databases

EnsembliENST00000206020; ENSP00000206020; ENSG00000091640.
GeneIDi9552.
KEGGihsa:9552.
UCSCiuc002gae.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047437 mRNA. Translation: AAC39888.1. Frameshift.
BC011934 mRNA. Translation: AAH11934.1.
CCDSiCCDS42240.1.
RefSeqiNP_004881.2. NM_004890.2.
UniGeneiHs.90436.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CPMNMR-A44-124[»]
ProteinModelPortaliO75391.
SMRiO75391. Positions 44-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114924. 3 interactions.
IntActiO75391. 1 interaction.
MINTiMINT-1033754.
STRINGi9606.ENSP00000206020.

PTM databases

PhosphoSiteiO75391.

Proteomic databases

MaxQBiO75391.
PaxDbiO75391.
PRIDEiO75391.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000206020; ENSP00000206020; ENSG00000091640.
GeneIDi9552.
KEGGihsa:9552.
UCSCiuc002gae.3. human.

Organism-specific databases

CTDi9552.
GeneCardsiGC17M004862.
HGNCiHGNC:11216. SPAG7.
HPAiHPA024032.
HPA052394.
MIMi610056. gene.
neXtProtiNX_O75391.
PharmGKBiPA36052.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG75013.
GeneTreeiENSGT00390000001520.
HOGENOMiHOG000234363.
HOVERGENiHBG066267.
InParanoidiO75391.
OMAiEESRYVM.
PhylomeDBiO75391.
TreeFamiTF323631.

Miscellaneous databases

ChiTaRSiSPAG7. human.
EvolutionaryTraceiO75391.
GeneWikiiSPAG7.
GenomeRNAii9552.
NextBioi35821.
PROiO75391.
SOURCEiSearch...

Gene expression databases

BgeeiO75391.
CleanExiHS_SPAG7.
ExpressionAtlasiO75391. baseline and differential.
GenevestigatoriO75391.

Family and domain databases

Gene3Di3.30.1370.50. 1 hit.
InterProiIPR001374. R3H_ss-bd.
IPR017330. Sperm-assoc_antigen_PAG7.
[Graphical view]
PfamiPF01424. R3H. 1 hit.
[Graphical view]
PIRSFiPIRSF037943. Sperm-assoc_antigen_PAG7. 1 hit.
SMARTiSM00393. R3H. 1 hit.
[Graphical view]
SUPFAMiSSF82708. SSF82708. 1 hit.
PROSITEiPS51061. R3H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
    Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.
    Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  3. "Cloning and characterization of a fox sperm protein FSA-1."
    Beaton S., Cleary A., ten Have J., Bradley M.P.
    Reprod. Fertil. Dev. 6:761-770(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Solution structure of the R3H domain of human sperm-associated antigen 7."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 44-124.

Entry informationi

Entry nameiSPAG7_HUMAN
AccessioniPrimary (citable) accession number: O75391
Secondary accession number(s): Q96EU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: January 7, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.