Reviewed,
UniProtKB/Swiss-Prot O75385 (ULK1_HUMAN)
Last modified
June 16, 2009.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase ULK1 EC=2.7.11.1 Alternative name(s): Unc-51-like kinase 1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1050 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Interacts with GABARAP and GABARAPL2. Ref.5 |
| Tissue specificity | Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Polymorphism |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | protein localization Inferred from mutant phenotype. Source: UniProtKB regulation of autophagyInferred from mutant phenotype. Source: UniProtKB |
| Cellular component | autophagic vacuole Inferred from direct assay. Source: UniProtKB cytosol Ref.5Inferred from direct assay. Source: UniProtKB |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.5Inferred from physical interaction. Source: UniProtKB protein serine/threonine kinase activity Ref.1Non-traceable author statement. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1050 | 1050 | Serine/threonine-protein kinase ULK1 | PRO_0000086780 | |||||
Regions | |||||||||
| Domain | 16 – 278 | 263 | Protein kinase | ||||||
| Nucleotide binding | 22 – 30 | 9 | ATP By similarity | ||||||
| Region | 287 – 416 | 130 | Interaction with GABARAP and GABARAPL2 | ||||||
| Compositional bias | 297 – 310 | 14 | Poly-Ser | ||||||
Sites | |||||||||
| Active site | 138 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 46 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 450 | 1 | Phosphoserine Ref.9 Ref.10 | ||||||
| Modified residue | 456 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 477 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 479 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 556 | 1 | Phosphoserine Ref.10 Ref.6 | ||||||
| Modified residue | 625 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 638 | 1 | Phosphoserine Ref.7 Ref.8 | ||||||
Natural variations | |||||||||
| Natural variant | 290 | 1 | V → M in an ovarian mucinous carcinoma sample; somatic mutation. Ref.11 | VAR_041274 | |||||
| Natural variant | 298 | 1 | S → L: dbSNP rs56364352. Ref.11 | VAR_041275 | |||||
| Natural variant | 478 | 1 | P → L: dbSNP rs12827141. Ref.11 | VAR_041276 | |||||
| Natural variant | 503 | 1 | T → M: dbSNP rs55824543. Ref.11 | VAR_041277 | |||||
| Natural variant | 665 | 1 | S → L: dbSNP rs55815560. Ref.11 | VAR_041278 | |||||
| Natural variant | 714 | 1 | P → L: dbSNP rs11546871. Ref.11 | VAR_041279 | |||||
| Natural variant | 784 | 1 | S → C in a lung adenocarcinoma sample; somatic mutation. Ref.11 | VAR_041280 | |||||
| Natural variant | 816 | 1 | A → T: dbSNP rs11609348. Ref.4 | VAR_054892 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human ULK1, a novel serine/threonine kinase related to UNC-51 kinase of Caenorhabditis elegans: cDNA cloning, expression, and chromosomal assignment." Kuroyanagi H., Yan J., Seki N., Yamanouchi Y., Suzuki Y., Takano T., Muramatsu M., Shirasawa T. Genomics 51:76-85(1998) [PubMed: 9693035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [3] | "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION. |
| [4] | "The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. Gibbs R.A.Nature 440:346-351(2006) [PubMed: 16541075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-816. |
| [5] | "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation." Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A. Brain Res. Mol. Brain Res. 85:1-12(2000) [PubMed: 11146101] [Abstract] Cited for: INTERACTION WITH GABARAP AND GABARAPL2. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-556, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H. Mol. Cell. Proteomics 6:537-547(2007) [PubMed: 17192257] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638, MASS SPECTROMETRY. |
| [8] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-638, MASS SPECTROMETRY. |
| [9] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; SER-477 AND SER-479, MASS SPECTROMETRY. |
| [10] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-450; THR-456 AND SER-556, MASS SPECTROMETRY. |
| [11] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-290; LEU-298; LEU-478; MET-503; LEU-665; LEU-714 AND CYS-784. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF045458 mRNA. Translation: AAC32326.1. AB018265 mRNA. Translation: BAA34442.2. Different initiation. AC131009 Genomic DNA. No translation available. | |
| IPI | IPI00289357. |
| RefSeq | NP_003556.1. |
| UniGene | Hs.47061 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1KV1 based on UniProtKB Q16539. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O75385. 2 interactions. |
PTM databases | |
| PhosphoSite | O75385. |
Proteomic databases | |
| PRIDE | O75385. |
Genome annotation databases | |
| Ensembl | ENSG00000177169. Homo sapiens. [Contig view] |
| GeneID | 8408. |
| KEGG | hsa:8408. |
Organism-specific databases | |
| GeneCards | GC12P130945. |
| H-InvDB | HIX0019606. |
| HGNC | HGNC:12558. ULK1. |
| MIM | 603168. gene. |
| PharmGKB | PA37198. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | O75385. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 247. |
Gene expression databases | |
| ArrayExpress | O75385. |
| Bgee | O75385. |
| CleanEx | HS_ULK1. |
| GermOnline | ENSG00000177169. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR016237. Ser/Thr_prot_kin_STPK_Ulk-1/2. IPR013336. Ser/Thr_prot_kinase_Unc-51/Ulk. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| PANTHER | PTHR22982:SF38. Ser/Thr_prot_kinase_Unc-51. 1 hit. |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PIRSF | PIRSF000580. Ser/Thr_PK_STPK_ULK-1/2. 1 hit. |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 31476. |
| SOURCE | Search... |
Entry information
| Entry name | ULK1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O75385 Secondary accession number(s): Q9UQ28 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 12 Human chromosome 12: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


