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Protein

Tripartite motif-containing protein 3

Gene

TRIM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in vesicular trafficking via its association with the CART complex (PubMed:15772161). The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Positively regulates motility of microtubule-dependent motor protein KIF21B (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 63RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri110 – 151B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • protein C-terminus binding Source: ProtInc
  • ubiquitin protein ligase activity Source: Ensembl
  • zinc ion binding Source: InterPro

GO - Biological processi

  • nervous system development Source: ProtInc
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-877300. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 3
Alternative name(s):
Brain-expressed RING finger protein
RING finger protein 22
RING finger protein 97
Gene namesi
Name:TRIM3
Synonyms:BERP, RNF22, RNF97
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:10064. TRIM3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • dendrite Source: UniProtKB-SubCell
  • early endosome Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell projection, Cytoplasm, Endosome, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10612.
OpenTargetsiENSG00000110171.
PharmGKBiPA35534.

Polymorphism and mutation databases

BioMutaiTRIM3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000561972 – 744Tripartite motif-containing protein 3Add BLAST743

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO75382.
MaxQBiO75382.
PaxDbiO75382.
PeptideAtlasiO75382.
PRIDEiO75382.

PTM databases

iPTMnetiO75382.
PhosphoSitePlusiO75382.

Expressioni

Tissue specificityi

Expressed in brain, heart, uterus and testis.

Gene expression databases

BgeeiENSG00000110171.
CleanExiHS_TRIM3.
ExpressionAtlasiO75382. baseline and differential.
GenevisibleiO75382. HS.

Organism-specific databases

HPAiHPA043396.
HPA048233.

Interactioni

Subunit structurei

Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3 (PubMed:15772161). Interacts with ZFYVE28/LST2 (PubMed:19460345). Interacts with KIF21B (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein C-terminus binding Source: ProtInc

Protein-protein interaction databases

BioGridi115858. 21 interactors.
IntActiO75382. 22 interactors.
STRINGi9606.ENSP00000340797.

Structurei

3D structure databases

ProteinModelPortaliO75382.
SMRiO75382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati317 – 418FilaminAdd BLAST102
Repeati473 – 516NHL 1Add BLAST44
Repeati520 – 563NHL 2Add BLAST44
Repeati564 – 605NHL 3Add BLAST42
Repeati609 – 652NHL 4Add BLAST44
Repeati656 – 699NHL 5Add BLAST44
Repeati700 – 743NHL 6Add BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 290Interaction with KIF21BBy similarityAdd BLAST289

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili153 – 224Sequence analysisAdd BLAST72

Domaini

The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 63RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri110 – 151B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITF9. Eukaryota.
ENOG410ZAM4. LUCA.
GeneTreeiENSGT00870000136392.
HOGENOMiHOG000220817.
HOVERGENiHBG054843.
InParanoidiO75382.
KOiK11997.
OMAiCLDRYRC.
OrthoDBiEOG091G06IK.
PhylomeDBiO75382.
TreeFamiTF331018.

Family and domain databases

CDDicd00162. RING. 1 hit.
Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
SMARTiView protein in SMART
SM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiView protein in PROSITE
PS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: O75382-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKREDSPGP EVQPMDKQFL VCSICLDRYQ CPKVLPCLHT FCERCLQNYI
60 70 80 90 100
PAQSLTLSCP VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPDGAHDPE
110 120 130 140 150
DPHPLSVVAG RPLSCPNHEG KTMEFYCEAC ETAMCGECRA GEHREHGTVL
160 170 180 190 200
LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI ALVGGISQQL QERKAEALAQ
210 220 230 240 250
ISAAFEDLEQ ALQQRKQALV SDLETICGAK QKVLQSQLDT LRQGQEHIGS
260 270 280 290 300
SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL
310 320 330 340 350
EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK
360 370 380 390 400
DGRLVRTGSA ELRAEITGPD GTRLPVPVVD HKNGTYELVY TARTEGELLL
410 420 430 440 450
SVLLYGQPVR GSPFRVRALR PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV
460 470 480 490 500
RRPSSMYSTG GKRKDNPIED ELVFRVGSRG REKGEFTNLQ GVSAASSGRI
510 520 530 540 550
VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA VDTNGDIIVA
560 570 580 590 600
DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF
610 620 630 640 650
TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS
660 670 680 690 700
ADGEFLFKFG SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG
710 720 730 740
SFLSYINTSA EPLYGPQGLA LTSDGHVVVA DAGNHCFKAY RYLQ
Length:744
Mass (Da):80,830
Last modified:November 25, 2008 - v2
Checksum:iFF9829CB52314DD2
GO
Isoform Beta (identifier: O75382-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-156: Missing.

Show »
Length:733
Mass (Da):79,610
Checksum:iA31439D4CDEBA6B7
GO
Isoform Gamma (identifier: O75382-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-302: Missing.

Show »
Length:665
Mass (Da):72,101
Checksum:i640D87E904DBDA4E
GO
Isoform 4 (identifier: O75382-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-119: Missing.
     120-120: G → M

Note: No experimental confirmation available.
Show »
Length:625
Mass (Da):67,726
Checksum:i8813BB2C040EB51C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113L → F in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti113L → F in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti113L → F in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti113L → F in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti236S → T in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti236S → T in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti236S → T in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti347T → A in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti347T → A in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti347T → A in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti347T → A in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti450V → L in BAH12882 (PubMed:14702039).Curated1
Sequence conflicti631N → S in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti631N → S in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti631N → S in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti631N → S in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti636I → V in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti636I → V in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti636I → V in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti636I → V in AAG53476 (PubMed:11331580).Curated1
Sequence conflicti646V → E in AAC24809 (PubMed:11170753).Curated1
Sequence conflicti646V → E in AAG53474 (PubMed:11331580).Curated1
Sequence conflicti646V → E in AAG53475 (PubMed:11331580).Curated1
Sequence conflicti646V → E in AAG53476 (PubMed:11331580).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052124298L → R. Corresponds to variant dbSNP:rs10128723Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0447801 – 119Missing in isoform 4. 1 PublicationAdd BLAST119
Alternative sequenceiVSP_044781120G → M in isoform 4. 1 Publication1
Alternative sequenceiVSP_005758146 – 156Missing in isoform Beta. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_005759224 – 302Missing in isoform Gamma. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045239 mRNA. Translation: AAC24809.1.
AF220020 mRNA. Translation: AAG53474.1.
AF220021 mRNA. Translation: AAG53475.1.
AF220022 mRNA. Translation: AAG53476.1.
AK291396 mRNA. Translation: BAF84085.1.
AK298843 mRNA. Translation: BAH12882.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68709.1.
BC096827 mRNA. Translation: AAH96827.1.
CCDSiCCDS58115.1. [O75382-4]
CCDS7764.1. [O75382-1]
RefSeqiNP_001234935.1. NM_001248006.1. [O75382-1]
NP_001234936.1. NM_001248007.1. [O75382-4]
NP_006449.2. NM_006458.3. [O75382-1]
NP_150594.2. NM_033278.3. [O75382-1]
XP_011518146.1. XM_011519844.1. [O75382-1]
XP_011518147.1. XM_011519845.2. [O75382-4]
XP_016872587.1. XM_017017098.1. [O75382-1]
XP_016872588.1. XM_017017099.1. [O75382-4]
UniGeneiHs.591992.

Genome annotation databases

EnsembliENST00000345851; ENSP00000340797; ENSG00000110171. [O75382-1]
ENST00000359518; ENSP00000352508; ENSG00000110171. [O75382-1]
ENST00000525074; ENSP00000433102; ENSG00000110171. [O75382-1]
ENST00000536344; ENSP00000445460; ENSG00000110171. [O75382-4]
GeneIDi10612.
KEGGihsa:10612.
UCSCiuc001mdh.4. human. [O75382-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRIM3_HUMAN
AccessioniPrimary (citable) accession number: O75382
Secondary accession number(s): B7Z5E6
, F5H2Q8, Q4V9L4, Q9C038, Q9C039
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: November 25, 2008
Last modified: June 7, 2017
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families