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O75382 (TRIM3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tripartite motif-containing protein 3
Alternative name(s):
Brain-expressed RING finger protein
RING finger protein 22
RING finger protein 97
Gene names
Name:TRIM3
Synonyms:BERP, RNF22, RNF97
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Subunit structure

Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) By similarity. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with ZFYVE28/LST2. Ref.7 Ref.10

Subcellular location

Cytoplasm By similarity. Early endosome Ref.7.

Tissue specificity

Expressed in brain, heart, uterus and testis.

Domain

The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades By similarity.

Sequence similarities

Belongs to the TRIM/RBCC family.

Contains 1 B box-type zinc finger.

Contains 1 filamin repeat.

Contains 6 NHL repeats.

Contains 1 RING-type zinc finger.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: O75382-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: O75382-2)

The sequence of this isoform differs from the canonical sequence as follows:
     146-156: Missing.
Isoform Gamma (identifier: O75382-3)

The sequence of this isoform differs from the canonical sequence as follows:
     224-302: Missing.
Isoform 4 (identifier: O75382-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-119: Missing.
     120-120: G → M
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 744743Tripartite motif-containing protein 3
PRO_0000056197

Regions

Repeat317 – 418102Filamin
Repeat473 – 51644NHL 1
Repeat520 – 56344NHL 2
Repeat564 – 60542NHL 3
Repeat609 – 65244NHL 4
Repeat656 – 69944NHL 5
Repeat700 – 74344NHL 6
Zinc finger22 – 6342RING-type
Zinc finger110 – 15142B box-type
Coiled coil153 – 22472 Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.9
Modified residue71Phosphoserine By similarity

Natural variations

Alternative sequence1 – 119119Missing in isoform 4.
VSP_044780
Alternative sequence1201G → M in isoform 4.
VSP_044781
Alternative sequence146 – 15611Missing in isoform Beta.
VSP_005758
Alternative sequence224 – 30279Missing in isoform Gamma.
VSP_005759
Natural variant2981L → R.
Corresponds to variant rs10128723 [ dbSNP | Ensembl ].
VAR_052124

Experimental info

Sequence conflict1131L → F in AAC24809. Ref.1
Sequence conflict1131L → F in AAG53474. Ref.2
Sequence conflict1131L → F in AAG53475. Ref.2
Sequence conflict1131L → F in AAG53476. Ref.2
Sequence conflict2361S → T in AAC24809. Ref.1
Sequence conflict2361S → T in AAG53474. Ref.2
Sequence conflict2361S → T in AAG53475. Ref.2
Sequence conflict3471T → A in AAC24809. Ref.1
Sequence conflict3471T → A in AAG53474. Ref.2
Sequence conflict3471T → A in AAG53475. Ref.2
Sequence conflict3471T → A in AAG53476. Ref.2
Sequence conflict4501V → L in BAH12882. Ref.3
Sequence conflict6311N → S in AAC24809. Ref.1
Sequence conflict6311N → S in AAG53474. Ref.2
Sequence conflict6311N → S in AAG53475. Ref.2
Sequence conflict6311N → S in AAG53476. Ref.2
Sequence conflict6361I → V in AAC24809. Ref.1
Sequence conflict6361I → V in AAG53474. Ref.2
Sequence conflict6361I → V in AAG53475. Ref.2
Sequence conflict6361I → V in AAG53476. Ref.2
Sequence conflict6461V → E in AAC24809. Ref.1
Sequence conflict6461V → E in AAG53474. Ref.2
Sequence conflict6461V → E in AAG53475. Ref.2
Sequence conflict6461V → E in AAG53476. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: FF9829CB52314DD2

FASTA74480,830
        10         20         30         40         50         60 
MAKREDSPGP EVQPMDKQFL VCSICLDRYQ CPKVLPCLHT FCERCLQNYI PAQSLTLSCP 

        70         80         90        100        110        120 
VCRQTSILPE QGVSALQNNF FISSLMEAMQ QAPDGAHDPE DPHPLSVVAG RPLSCPNHEG 

       130        140        150        160        170        180 
KTMEFYCEAC ETAMCGECRA GEHREHGTVL LRDVVEQHKA ALQRQLEAVR GRLPQLSAAI 

       190        200        210        220        230        240 
ALVGGISQQL QERKAEALAQ ISAAFEDLEQ ALQQRKQALV SDLETICGAK QKVLQSQLDT 

       250        260        270        280        290        300 
LRQGQEHIGS SCSFAEQALR LGSAPEVLLV RKHMRERLAA LAAQAFPERP HENAQLELVL 

       310        320        330        340        350        360 
EVDGLRRSVL NLGALLTTSA TAHETVATGE GLRQALVGQP ASLTVTTKDK DGRLVRTGSA 

       370        380        390        400        410        420 
ELRAEITGPD GTRLPVPVVD HKNGTYELVY TARTEGELLL SVLLYGQPVR GSPFRVRALR 

       430        440        450        460        470        480 
PGDLPPSPDD VKRRVKSPGG PGSHVRQKAV RRPSSMYSTG GKRKDNPIED ELVFRVGSRG 

       490        500        510        520        530        540 
REKGEFTNLQ GVSAASSGRI VVADSNNQCI QVFSNEGQFK FRFGVRGRSP GQLQRPTGVA 

       550        560        570        580        590        600 
VDTNGDIIVA DYDNRWVSIF SPEGKFKTKI GAGRLMGPKG VAVDRNGHII VVDNKSCCVF 

       610        620        630        640        650        660 
TFQPNGKLVG RFGGRGATDR HFAGPHFVAV NNKNEIVVTD FHNHSVKVYS ADGEFLFKFG 

       670        680        690        700        710        720 
SHGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDSSG SFLSYINTSA EPLYGPQGLA 

       730        740 
LTSDGHVVVA DAGNHCFKAY RYLQ 

« Hide

Isoform Beta [UniParc].

Checksum: A31439D4CDEBA6B7
Show »

FASTA73379,610
Isoform Gamma [UniParc].

Checksum: 640D87E904DBDA4E
Show »

FASTA66572,101
Isoform 4 [UniParc].

Checksum: 8813BB2C040EB51C
Show »

FASTA62567,726

References

« Hide 'large scale' references
[1]"Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5."
El-Husseini A.E.-D., Fretier P., Vincent S.R.
Genomics 71:363-367(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA).
Tissue: Brain.
[2]"The tripartite motif family identifies cell compartments."
Reymond A., Meroni G., Fantozzi A., Merla G., Cairo S., Luzi L., Riganelli D., Zanaria E., Messali S., Cainarca S., Guffanti A., Minucci S., Pelicci P.G., Ballabio A.
EMBO J. 20:2140-2151(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA; BETA AND GAMMA).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS ALPHA AND 4).
Tissue: Brain.
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA).
Tissue: Cervix.
[7]"CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling."
Yan Q., Sun W., Kujala P., Lotfi Y., Vida T.A., Bean A.J.
Mol. Biol. Cell 16:2470-2482(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CART COMPLEX, SUBCELLULAR LOCATION.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[10]"Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling."
Mosesson Y., Chetrit D., Schley L., Berghoff J., Ziv T., Carvalho S., Milanezi F., Admon A., Schmitt F., Ehrlich M., Yarden Y.
Dev. Cell 16:687-698(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZFYVE28.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF045239 mRNA. Translation: AAC24809.1.
AF220020 mRNA. Translation: AAG53474.1.
AF220021 mRNA. Translation: AAG53475.1.
AF220022 mRNA. Translation: AAG53476.1.
AK291396 mRNA. Translation: BAF84085.1.
AK298843 mRNA. Translation: BAH12882.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68709.1.
BC096827 mRNA. Translation: AAH96827.1.
CCDSCCDS58115.1. [O75382-4]
CCDS7764.1. [O75382-1]
RefSeqNP_001234935.1. NM_001248006.1. [O75382-1]
NP_001234936.1. NM_001248007.1. [O75382-4]
NP_006449.2. NM_006458.3. [O75382-1]
NP_150594.2. NM_033278.3. [O75382-1]
UniGeneHs.591992.

3D structure databases

ProteinModelPortalO75382.
SMRO75382. Positions 18-63, 290-420, 475-744.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115858. 10 interactions.
IntActO75382. 3 interactions.
STRING9606.ENSP00000340797.

PTM databases

PhosphoSiteO75382.

Proteomic databases

MaxQBO75382.
PaxDbO75382.
PRIDEO75382.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000345851; ENSP00000340797; ENSG00000110171. [O75382-1]
ENST00000359518; ENSP00000352508; ENSG00000110171. [O75382-1]
ENST00000525074; ENSP00000433102; ENSG00000110171. [O75382-1]
ENST00000536344; ENSP00000445460; ENSG00000110171. [O75382-4]
GeneID10612.
KEGGhsa:10612.
UCSCuc001mdh.3. human. [O75382-1]

Organism-specific databases

CTD10612.
GeneCardsGC11M006469.
HGNCHGNC:10064. TRIM3.
HPAHPA043396.
HPA048233.
MIM605493. gene.
neXtProtNX_O75382.
PharmGKBPA35534.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG3391.
HOGENOMHOG000220817.
HOVERGENHBG054843.
InParanoidO75382.
KOK11997.
OMAINTTAEP.
OrthoDBEOG7H4DST.
PhylomeDBO75382.
TreeFamTF331018.

Gene expression databases

ArrayExpressO75382.
BgeeO75382.
CleanExHS_TRIM3.
GenevestigatorO75382.

Family and domain databases

Gene3D2.120.10.30. 1 hit.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000315. Znf_B-box.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTSM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF81296. SSF81296. 1 hit.
PROSITEPS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTRIM3.
GenomeRNAi10612.
NextBio40314.
PROO75382.
SOURCESearch...

Entry information

Entry nameTRIM3_HUMAN
AccessionPrimary (citable) accession number: O75382
Secondary accession number(s): B7Z5E6 expand/collapse secondary AC list , F5H2Q8, Q4V9L4, Q9C038, Q9C039
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: November 25, 2008
Last modified: July 9, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM