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Protein

Vesicle-associated membrane protein 4

Gene

VAMP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.

GO - Molecular functioni

GO - Biological processi

  • exocytosis Source: GO_Central
  • Golgi ribbon formation Source: UniProtKB
  • microtubule cytoskeleton organization Source: UniProtKB
  • SNARE complex assembly Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000117533-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.
SIGNORiO75379.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 4
Short name:
VAMP-4
Gene namesi
Name:VAMP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:12645. VAMP4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 115CytoplasmicSequence analysisAdd BLAST115
Transmembranei116 – 136Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini137 – 141VesicularSequence analysis5

GO - Cellular componenti

  • cell surface Source: BHF-UCL
  • endosome Source: ProtInc
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: ProtInc
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: ProtInc
  • SNARE complex Source: GO_Central
  • trans-Golgi network membrane Source: Reactome
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8674.
OpenTargetsiENSG00000117533.
PharmGKBiPA37269.

Polymorphism and mutation databases

BioMutaiVAMP4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067311 – 141Vesicle-associated membrane protein 4Add BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineBy similarity1
Modified residuei30PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO75379.
PaxDbiO75379.
PeptideAtlasiO75379.
PRIDEiO75379.

PTM databases

iPTMnetiO75379.
PhosphoSitePlusiO75379.
SwissPalmiO75379.

Expressioni

Gene expression databases

BgeeiENSG00000117533.
CleanExiHS_VAMP4.
ExpressionAtlasiO75379. baseline and differential.
GenevisibleiO75379. HS.

Organism-specific databases

HPAiHPA050418.

Interactioni

Subunit structurei

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2L13Q9BXK53EBI-10187996,EBI-747430
CCDC155Q8N6L03EBI-744953,EBI-749265
SNAP29O957213EBI-10187996,EBI-490676
SNAP47Q5SQN12EBI-744953,EBI-10244848
STX4Q128466EBI-744953,EBI-744942

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114222. 48 interactors.
DIPiDIP-44226N.
IntActiO75379. 22 interactors.
MINTiMINT-1469104.
STRINGi9606.ENSP00000236192.

Structurei

3D structure databases

ProteinModelPortaliO75379.
SMRiO75379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 112v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG061695.
InParanoidiO75379.
KOiK08513.
OMAiWRGCKMK.
OrthoDBiEOG091G0MPE.
PhylomeDBiO75379.
TreeFamiTF313666.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75379-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN
60 70 80 90 100
DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN
110 120 130 140
RSKQLRRQMW WRGCKIKAIM ALVAAILLLV IIILIVMKYR T
Length:141
Mass (Da):16,397
Last modified:April 3, 2002 - v2
Checksum:i556CC682D26CF91E
GO
Isoform 2 (identifier: O75379-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-39: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):16,241
Checksum:iA2D4E66093D57837
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68Q → P in AAC24032 (PubMed:9553086).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00632639Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044310 mRNA. Translation: AAC24032.1.
AL035296 mRNA. Translation: CAA22896.1.
Z98751 Genomic DNA. Translation: CAM28272.1.
CH471067 Genomic DNA. Translation: EAW90904.1.
BC005974 mRNA. Translation: AAH05974.1.
BC007019 mRNA. Translation: AAH07019.1.
BC031264 mRNA. Translation: AAH31264.1.
CCDSiCCDS1298.1. [O75379-1]
CCDS53430.1. [O75379-2]
RefSeqiNP_001172056.1. NM_001185127.1. [O75379-2]
NP_003753.2. NM_003762.4. [O75379-1]
UniGeneiHs.6651.

Genome annotation databases

EnsembliENST00000236192; ENSP00000236192; ENSG00000117533. [O75379-1]
ENST00000367740; ENSP00000356714; ENSG00000117533. [O75379-2]
ENST00000474047; ENSP00000435933; ENSG00000117533. [O75379-1]
GeneIDi8674.
KEGGihsa:8674.
UCSCiuc001ghy.3. human. [O75379-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044310 mRNA. Translation: AAC24032.1.
AL035296 mRNA. Translation: CAA22896.1.
Z98751 Genomic DNA. Translation: CAM28272.1.
CH471067 Genomic DNA. Translation: EAW90904.1.
BC005974 mRNA. Translation: AAH05974.1.
BC007019 mRNA. Translation: AAH07019.1.
BC031264 mRNA. Translation: AAH31264.1.
CCDSiCCDS1298.1. [O75379-1]
CCDS53430.1. [O75379-2]
RefSeqiNP_001172056.1. NM_001185127.1. [O75379-2]
NP_003753.2. NM_003762.4. [O75379-1]
UniGeneiHs.6651.

3D structure databases

ProteinModelPortaliO75379.
SMRiO75379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114222. 48 interactors.
DIPiDIP-44226N.
IntActiO75379. 22 interactors.
MINTiMINT-1469104.
STRINGi9606.ENSP00000236192.

PTM databases

iPTMnetiO75379.
PhosphoSitePlusiO75379.
SwissPalmiO75379.

Polymorphism and mutation databases

BioMutaiVAMP4.

Proteomic databases

EPDiO75379.
PaxDbiO75379.
PeptideAtlasiO75379.
PRIDEiO75379.

Protocols and materials databases

DNASUi8674.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000236192; ENSP00000236192; ENSG00000117533. [O75379-1]
ENST00000367740; ENSP00000356714; ENSG00000117533. [O75379-2]
ENST00000474047; ENSP00000435933; ENSG00000117533. [O75379-1]
GeneIDi8674.
KEGGihsa:8674.
UCSCiuc001ghy.3. human. [O75379-1]

Organism-specific databases

CTDi8674.
DisGeNETi8674.
GeneCardsiVAMP4.
HGNCiHGNC:12645. VAMP4.
HPAiHPA050418.
MIMi606909. gene.
neXtProtiNX_O75379.
OpenTargetsiENSG00000117533.
PharmGKBiPA37269.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG061695.
InParanoidiO75379.
KOiK08513.
OMAiWRGCKMK.
OrthoDBiEOG091G0MPE.
PhylomeDBiO75379.
TreeFamiTF313666.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000117533-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.
SIGNORiO75379.

Miscellaneous databases

GeneWikiiVAMP4.
GenomeRNAii8674.
PROiO75379.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117533.
CleanExiHS_VAMP4.
ExpressionAtlasiO75379. baseline and differential.
GenevisibleiO75379. HS.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP4_HUMAN
AccessioniPrimary (citable) accession number: O75379
Secondary accession number(s): A2IDD8
, Q96IY9, Q96J20, Q9UEL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 3, 2002
Last modified: November 30, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.