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Protein

Protein tyrosine phosphatase type IVA 3

Gene

PTP4A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Enhances cell proliferation, cell motility and invasive activity, and promotes cancer metastasis. May be involved in the progression of cardiac hypertrophy by inhibiting intracellular calcium mobilization in response to angiotensin II.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Inhibited by sodium orthovanadate and peroxovanadium compounds, and by pentamidine.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72Proton donorCurated1
Active sitei104Phosphocysteine intermediate1
Binding sitei110Substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:HS00003-MONOMER.
SIGNORiO75365.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein tyrosine phosphatase type IVA 3 (EC:3.1.3.48)
Alternative name(s):
PRL-R
Protein-tyrosine phosphatase 4a3
Protein-tyrosine phosphatase of regenerating liver 3
Short name:
PRL-3
Gene namesi
Name:PTP4A3
Synonyms:PRL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:9636. PTP4A3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49C → A: No effect on enzymatic activity. 1 Publication1
Mutagenesisi71D → A: No effect on enzymatic activity. 1 Publication1
Mutagenesisi72D → A: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi104C → A or S: 95% loss of enzymatic activity. 2 Publications1
Mutagenesisi104C → S: Reduces migration-promoting activity. 2 Publications1
Mutagenesisi111A → S: Enhances catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi11156.
OpenTargetsiENSG00000184489.
ENSG00000275575.
PharmGKBiPA33979.

Chemistry databases

ChEMBLiCHEMBL4162.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947881 – 170Protein tyrosine phosphatase type IVA 3Add BLAST170
PropeptideiPRO_0000396735171 – 173Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 1041 Publication
Modified residuei170Cysteine methyl esterBy similarity1
Lipidationi170S-farnesyl cysteineBy similarity1

Post-translational modificationi

Farnesylated. Farnesylation is required for membrane targeting (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiO75365.
PaxDbiO75365.
PeptideAtlasiO75365.
PRIDEiO75365.

PTM databases

DEPODiO75365.
iPTMnetiO75365.
PhosphoSitePlusiO75365.

Expressioni

Tissue specificityi

Mainly expressed in cardiomyocytes and skeletal muscle; also found in pancreas. Consistently overexpressed in colon cancer metastasis.1 Publication

Gene expression databases

BgeeiENSG00000184489.
CleanExiHS_PTP4A3.
ExpressionAtlasiO75365. baseline and differential.
GenevisibleiO75365. HS.

Interactioni

Subunit structurei

Interacts with tubulin.1 Publication

Protein-protein interaction databases

BioGridi116327. 39 interactors.
IntActiO75365. 4 interactors.
STRINGi9606.ENSP00000332274.

Chemistry databases

BindingDBiO75365.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 14Combined sources5
Beta strandi17 – 22Combined sources6
Beta strandi23 – 26Combined sources4
Turni27 – 29Combined sources3
Helixi30 – 40Combined sources11
Beta strandi45 – 49Combined sources5
Helixi55 – 61Combined sources7
Beta strandi65 – 68Combined sources4
Beta strandi69 – 71Combined sources3
Helixi79 – 94Combined sources16
Beta strandi99 – 103Combined sources5
Beta strandi107 – 110Combined sources4
Helixi111 – 119Combined sources9
Helixi120 – 122Combined sources3
Helixi125 – 135Combined sources11
Beta strandi137 – 139Combined sources3
Helixi144 – 152Combined sources9
Turni156 – 158Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R6HNMR-A1-169[»]
1V3ANMR-A1-173[»]
2MBCNMR-A1-162[»]
DisProtiDP00254.
ProteinModelPortaliO75365.
SMRiO75365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO75365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 148Tyrosine-protein phosphataseAdd BLAST67

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2836. Eukaryota.
ENOG4111I7J. LUCA.
GeneTreeiENSGT00390000009788.
HOGENOMiHOG000231265.
HOVERGENiHBG071295.
InParanoidiO75365.
KOiK18041.
OMAiKAKFCDD.
OrthoDBiEOG091G0MYM.
PhylomeDBiO75365.
TreeFamiTF313384.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O75365-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARMNRPAPV EVSYKHMRFL ITHNPTNATL STFIEDLKKY GATTVVRVCE
60 70 80 90 100
VTYDKTPLEK DGITVVDWPF DDGAPPPGKV VEDWLSLVKA KFCEAPGSCV
110 120 130 140 150
AVHCVAGLGR APVLVALALI ESGMKYEDAI QFIRQKRRGA INSKQLTYLE
160 170
KYRPKQRLRF KDPHTHKTRC CVM
Length:173
Mass (Da):19,535
Last modified:July 5, 2005 - v2
Checksum:i15DF01999A9A3573
GO
Isoform 2 (identifier: O75365-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-135: Missing.

Note: Unstructured and inactive.
Show »
Length:148
Mass (Da):16,777
Checksum:iE9B7DB82575DF7CD
GO
Isoform 3 (identifier: O75365-3) [UniParc]FASTAAdd to basket
Also known as: short

The sequence of this isoform differs from the canonical sequence as follows:
     39-124: Missing.

Show »
Length:87
Mass (Da):10,494
Checksum:iDE4A4D64102DC8CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140A → R in AAC29314 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01440639 – 124Missing in isoform 3. 1 PublicationAdd BLAST86
Alternative sequenceiVSP_014407111 – 135Missing in isoform 2. 2 PublicationsAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041434 mRNA. Translation: AAC29314.1.
AJ276554 mRNA. Translation: CAC81757.1.
BT007303 mRNA. Translation: AAP35967.1.
BC003105 mRNA. Translation: AAH03105.1.
U87168 mRNA. Translation: AAB47560.1.
CCDSiCCDS6382.1. [O75365-2]
CCDS6383.1. [O75365-1]
RefSeqiNP_009010.2. NM_007079.3. [O75365-2]
NP_116000.1. NM_032611.2. [O75365-1]
XP_005250821.1. XM_005250764.2. [O75365-1]
XP_011515105.1. XM_011516803.1. [O75365-1]
XP_011515107.1. XM_011516805.1. [O75365-1]
XP_016868487.1. XM_017012998.1. [O75365-2]
UniGeneiHs.43666.
Hs.744870.

Genome annotation databases

EnsembliENST00000329397; ENSP00000332274; ENSG00000184489. [O75365-1]
ENST00000349124; ENSP00000331730; ENSG00000184489. [O75365-2]
ENST00000520105; ENSP00000428758; ENSG00000184489. [O75365-2]
ENST00000521578; ENSP00000428976; ENSG00000184489. [O75365-1]
ENST00000614325; ENSP00000481205; ENSG00000275575. [O75365-2]
ENST00000622569; ENSP00000484500; ENSG00000275575. [O75365-1]
ENST00000633518; ENSP00000487595; ENSG00000275575. [O75365-1]
ENST00000633621; ENSP00000487610; ENSG00000275575. [O75365-2]
GeneIDi11156.
KEGGihsa:11156.
UCSCiuc003ywg.2. human. [O75365-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041434 mRNA. Translation: AAC29314.1.
AJ276554 mRNA. Translation: CAC81757.1.
BT007303 mRNA. Translation: AAP35967.1.
BC003105 mRNA. Translation: AAH03105.1.
U87168 mRNA. Translation: AAB47560.1.
CCDSiCCDS6382.1. [O75365-2]
CCDS6383.1. [O75365-1]
RefSeqiNP_009010.2. NM_007079.3. [O75365-2]
NP_116000.1. NM_032611.2. [O75365-1]
XP_005250821.1. XM_005250764.2. [O75365-1]
XP_011515105.1. XM_011516803.1. [O75365-1]
XP_011515107.1. XM_011516805.1. [O75365-1]
XP_016868487.1. XM_017012998.1. [O75365-2]
UniGeneiHs.43666.
Hs.744870.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R6HNMR-A1-169[»]
1V3ANMR-A1-173[»]
2MBCNMR-A1-162[»]
DisProtiDP00254.
ProteinModelPortaliO75365.
SMRiO75365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116327. 39 interactors.
IntActiO75365. 4 interactors.
STRINGi9606.ENSP00000332274.

Chemistry databases

BindingDBiO75365.
ChEMBLiCHEMBL4162.

PTM databases

DEPODiO75365.
iPTMnetiO75365.
PhosphoSitePlusiO75365.

Proteomic databases

MaxQBiO75365.
PaxDbiO75365.
PeptideAtlasiO75365.
PRIDEiO75365.

Protocols and materials databases

DNASUi11156.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329397; ENSP00000332274; ENSG00000184489. [O75365-1]
ENST00000349124; ENSP00000331730; ENSG00000184489. [O75365-2]
ENST00000520105; ENSP00000428758; ENSG00000184489. [O75365-2]
ENST00000521578; ENSP00000428976; ENSG00000184489. [O75365-1]
ENST00000614325; ENSP00000481205; ENSG00000275575. [O75365-2]
ENST00000622569; ENSP00000484500; ENSG00000275575. [O75365-1]
ENST00000633518; ENSP00000487595; ENSG00000275575. [O75365-1]
ENST00000633621; ENSP00000487610; ENSG00000275575. [O75365-2]
GeneIDi11156.
KEGGihsa:11156.
UCSCiuc003ywg.2. human. [O75365-1]

Organism-specific databases

CTDi11156.
DisGeNETi11156.
GeneCardsiPTP4A3.
HGNCiHGNC:9636. PTP4A3.
MIMi606449. gene.
neXtProtiNX_O75365.
OpenTargetsiENSG00000184489.
ENSG00000275575.
PharmGKBiPA33979.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2836. Eukaryota.
ENOG4111I7J. LUCA.
GeneTreeiENSGT00390000009788.
HOGENOMiHOG000231265.
HOVERGENiHBG071295.
InParanoidiO75365.
KOiK18041.
OMAiKAKFCDD.
OrthoDBiEOG091G0MYM.
PhylomeDBiO75365.
TreeFamiTF313384.

Enzyme and pathway databases

BioCyciZFISH:HS00003-MONOMER.
SIGNORiO75365.

Miscellaneous databases

ChiTaRSiPTP4A3. human.
EvolutionaryTraceiO75365.
GeneWikiiPTP4A3.
GenomeRNAii11156.
PROiO75365.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184489.
CleanExiHS_PTP4A3.
ExpressionAtlasiO75365. baseline and differential.
GenevisibleiO75365. HS.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTP4A3_HUMAN
AccessioniPrimary (citable) accession number: O75365
Secondary accession number(s): Q8IVN5, Q99849, Q9BTW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.