O75362 (ZN217_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 217

Gene names

Name:	ZNF217
Synonyms:	ZABC1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1048 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'. Ref.6 Ref.7 Ref.8 Ref.11
Subunit structure	Component of a histone deacetylase complex that contains HDAC2, KDM1A, CTBP1 and ZNF217. May be a component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST, PHF21A/BHC80, ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with CTBP1 and CTBP2. Ref.5 Ref.8 Ref.11
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-templated Inferred from direct assay Ref.11. Source: UniProtKB regulation of transcription, DNA-templated Inferred from direct assay Ref.8. Source: UniProtKB transcription, DNA-templated Traceable author statement Ref.1. Source: ProtInc
Cellular_component	histone deacetylase complex Inferred from direct assay Ref.8. Source: UniProtKB nucleoplasm Inferred from direct assay Ref.8. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.11. Source: UniProtKB sequence-specific DNA binding transcription factor activity Traceable author statement Ref.1. Source: ProtInc transcription regulatory region DNA binding Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1048

1048

Zinc finger protein 217

PRO_0000047460

Regions

Zinc finger

65 – 88

24

C2H2-type 1

Zinc finger

128 – 150

23

C2H2-type 2

Zinc finger

156 – 178

23

C2H2-type 3

Zinc finger

216 – 238

23

C2H2-type 4

Zinc finger

375 – 397

23

C2H2-type 5

Zinc finger

471 – 493

23

C2H2-type 6

Zinc finger

499 – 521

23

C2H2-type 7

Amino acid modifications

Modified residue

321

1

Phosphoserine Ref.9

Modified residue

322

1

Phosphothreonine Ref.9

Modified residue

407

1

Phosphoserine Ref.10

Modified residue

648

1

Phosphothreonine Ref.9

Modified residue

662

1

Phosphoserine Ref.9

Modified residue

795

1

Phosphoserine Ref.10

Natural variations

Natural variant

323

1

D → N in a colorectal cancer sample; somatic mutation. Ref.12

VAR_035572

Natural variant

739

1

V → I.
Corresponds to variant rs6063966 [ dbSNP | Ensembl ].

VAR_052795

Natural variant

889

1

D → G.
Corresponds to variant rs34323943 [ dbSNP | Ensembl ].

VAR_061939

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

1048

Helix Strand Turn

Details...

Turn

474 – 476

3

Helix

483 – 494

12

Beta strand

502 – 505

4

Beta strand

507 – 510

4

Helix

511 – 521

11

Sequences

	Sequence		Length	Mass (Da)
	O75362 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 797FC620817D1E1F	FASTA	1,048	115,272
	10 20 30 40 50 60 MQSKVTGNMP TQSLLMYMDG PEVIGSSLGS PMEMEDALSM KGTAVVPFRA TQEKNVIQIE 70 80 90 100 110 120 GYMPLDCMFC SQTFTHSEDL NKHVLMQHRP TLCEPAVLRV EAEYLSPLDK SQVRTEPPKE 130 140 150 160 170 180 KNCKENEFSC EVCGQTFRVA FDVEIHMRTH KDSFTYGCNM CGRRFKEPWF LKNHMRTHNG 190 200 210 220 230 240 KSGARSKLQQ GLESSPATIN EVVQVHAAES ISSPYKICMV CGFLFPNKES LIEHRKVHTK 250 260 270 280 290 300 KTAFGTSSAQ TDSPQGGMPS SREDFLQLFN LRPKSHPETG KKPVRCIPQL DPFTTFQAWQ 310 320 330 340 350 360 LATKGKVAIC QEVKESGQEG STDNDDSSSE KELGETNKGS CAGLSQEKEK CKHSHGEAPS 370 380 390 400 410 420 VDADPKLPSS KEKPTHCSEC GKAFRTYHQL VLHSRVHKKD RRAGAESPTM SVDGRQPGTC 430 440 450 460 470 480 SPDLAAPLDE NGAVDRGEGG SEDGSEDGLP EGIHLDKNDD GGKIKHLTSS RECSYCGKFF 490 500 510 520 530 540 RSNYYLNIHL RTHTGEKPYK CEFCEYAAAQ KTSLRYHLER HHKEKQTDVA AEVKNDGKNQ 550 560 570 580 590 600 DTEDALLTAD SAQTKNLKRF FDGAKDVTGS PPAKQLKEMP SVFQNVLGSA VLSPAHKDTQ 610 620 630 640 650 660 DFHKNAADDS ADKVNKNPTP AYLDLLKKRS AVETQANNLI CRTKADVTPP PDGSTTHNLE 670 680 690 700 710 720 VSPKEKQTET AADCRYRPSV DCHEKPLNLS VGALHNCPAI SLSKSLIPSI TCPFCTFKTF 730 740 750 760 770 780 YPEVLMMHQR LEHKYNPDVH KNCRNKSLLR SRRTGCPPAL LGKDVPPLSS FCKPKPKSAF 790 800 810 820 830 840 PAQSKSLPSA KGKQSPPGPG KAPLTSGIDS STLAPSNLKS HRPQQNVGVQ GAATRQQQSE 850 860 870 880 890 900 MFPKTSVSPA PDKTKRPETK LKPLPVAPSQ PTLGSSNING SIDYPAKNDS PWAPPGRDYF 910 920 930 940 950 960 CNRSASNTAA EFGEPLPKRL KSSVVALDVD QPGANYRRGY DLPKYHMVRG ITSLLPQDCV 970 980 990 1000 1010 1020 YPSQALPPKP RFLSSSEVDS PNVLTVQKPY GGSGPLYTCV PAGSPASSST LEGKRPVSYQ 1030 1040 HLSNSMAQKR NYENFIGNAH YRPNDKKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma." Collins C., Rommens J.M., Kowbel D., Godfrey T., Tanner M., Hwang S.-I., Polikoff D., Nonet G., Cochran J., Myambo K., Jay K.E., Froula J., Cloutier T., Kuo W.-L., Yaswen P., Dairkee S., Giovanola J., Hutchinson G.B. , Isola J., Kallioniemi O.-P., Palazzolo M., Martin C., Ericsson C., Pinkel D., Albertson D., Li W.-B., Gray J.W. Proc. Natl. Acad. Sci. U.S.A. 95:8703-8708(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[5]	"A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes." Hakimi M.-A., Dong Y., Lane W.S., Speicher D.W., Shiekhattar R. J. Biol. Chem. 278:7234-7239(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE BHC COMPLEX WITH HDAC1; HDAC2; HMG20B; KDM1A; RCOR1; PHF21A; ZMYM2; ZMYM3; KIAA0182 AND GTF2I.
[6]	"ZNF217 suppresses cell death associated with chemotherapy and telomere dysfunction." Huang G., Krig S., Kowbel D., Xu H., Hyun B., Volik S., Feuerstein B., Mills G.B., Stokoe D., Yaswen P., Collins C. Hum. Mol. Genet. 14:3219-3225(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Identification of genes directly regulated by the oncogene ZNF217 using chromatin immunoprecipitation (ChIP)-chip assays." Krig S.R., Jin V.X., Bieda M.C., O'Geen H., Yaswen P., Green R., Farnham P.J. J. Biol. Chem. 282:9703-9712(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Genome analysis identifies the p15ink4b tumor suppressor as a direct target of the ZNF217/CoREST complex." Thillainadesan G., Isovic M., Loney E., Andrews J., Tini M., Torchia J. Mol. Cell. Biol. 28:6066-6077(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, IDENTIFICATION IN A COMPLEX WITH HDAC2; KDM1A AND CTBP1, FUNCTION.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321; THR-322; THR-648 AND SER-662, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407 AND SER-795, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Specific recognition of ZNF217 and other zinc finger proteins at a surface groove of C-terminal binding proteins." Quinlan K.G.R., Nardini M., Verger A., Francescato P., Yaswen P., Corda D., Bolognesi M., Crossley M. Mol. Cell. Biol. 26:8159-8172(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 752-760 IN COMPLEX WITH CTBP1, INTERACTION WITH CTBP1 AND CTBP2, FUNCTION.
[12]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-323.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF041259 mRNA. Translation: AAC39895.1.
AL157838 Genomic DNA. Translation: CAC08433.1.
CH471077 Genomic DNA. Translation: EAW75580.1.
BC113427 mRNA. Translation: AAI13428.1.

CCDS

CCDS13443.1.

RefSeq

NP_006517.1. NM_006526.2.

UniGene

Hs.155040.
Hs.711564.

3D structure databases

PDBe
RCSB-PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HU2	X-ray	2.85	B	752-760	[»]
3UK3	X-ray	2.10	C/D	469-523	[»]
4F2J	X-ray	2.64	C	469-523	[»]
4IS1	X-ray	2.10	C/D	469-523	[»]

ProteinModelPortal

O75362.

SMR

O75362. Positions 58-93, 114-268, 368-403, 469-551.

ModBase

Search...

MobiDB

Search...

Protein-protein interaction databases

BioGrid

113547. 12 interactions.

IntAct

O75362. 3 interactions.

MINT

MINT-7970236.

STRING

9606.ENSP00000304308.

PTM databases

PhosphoSite

O75362.

Proteomic databases

MaxQB

O75362.

PaxDb

O75362.

PRIDE

O75362.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000302342; ENSP00000304308; ENSG00000171940.
ENST00000371471; ENSP00000360526; ENSG00000171940.

GeneID

7764.

KEGG

hsa:7764.

UCSC

uc002xwq.4. human.

Organism-specific databases

CTD

7764.

GeneCards

GC20M052183.

HGNC

HGNC:13009. ZNF217.

HPA

HPA051857.

MIM

602967. gene.

neXtProt

NX_O75362.

PharmGKB

PA37588.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG319981.

HOGENOM

HOG000276541.

HOVERGEN

HBG061769.

InParanoid

O75362.

OMA

TGNMPTQ.

OrthoDB

EOG7CNZFG.

PhylomeDB

O75362.

TreeFam

TF332241.

Gene expression databases

ArrayExpress

O75362.

Bgee

O75362.

CleanEx

HS_ZNF217.

Genevestigator

O75362.

Family and domain databases

Gene3D

3.30.160.60. 4 hits.

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Pfam

PF00096. zf-C2H2. 2 hits.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 8 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 6 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

ZNF217. human.

EvolutionaryTrace

O75362.

GeneWiki

ZNF217.

GenomeRNAi

7764.

NextBio

30059.

PRO

O75362.

SOURCE

Search...

Entry information

Entry name

ZN217_HUMAN

Accession

Primary (citable) accession number: O75362
Secondary accession number(s): E1P5Y6, Q14DB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1998
Last modified:	July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.