Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ectonucleoside triphosphate diphosphohydrolase 3

Gene

ENTPD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a threefold preference for the hydrolysis of ATP over ADP.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei182Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS09682-MONOMER.
BRENDAi3.1.3.31. 2681.
ReactomeiR-HSA-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 3 (EC:3.6.1.5)
Short name:
NTPDase 3
Alternative name(s):
CD39 antigen-like 3
Ecto-ATP diphosphohydrolase 3
Short name:
Ecto-ATPDase 3
Short name:
Ecto-ATPase 3
Ecto-apyrase 3
HB6
Gene namesi
Name:ENTPD3
Synonyms:CD39L3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:3365. ENTPD3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 485ExtracellularSequence analysisAdd BLAST442
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Topological domaini507 – 529CytoplasmicSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67R → G: Increase of activity. 1 Publication1
Mutagenesisi143R → A: Loss of activity. 1 Publication1
Mutagenesisi143R → K: Increase of activity. 1 Publication1
Mutagenesisi146R → N: No effect. 1 Publication1
Mutagenesisi146R → P: Increase of ATPase activity, decrease of ADPase activity. 1 Publication1
Mutagenesisi146R → T: Increase of activity. 1 Publication1
Mutagenesisi182E → D: Complete loss of activity. 1 Publication1
Mutagenesisi182E → Q: Complete loss of activity. 1 Publication1
Mutagenesisi187W → A: Complete loss of activity. 1 Publication1
Mutagenesisi191N → A: Loss of ATPase activity, increase of ADPase activity. 1 Publication1
Mutagenesisi219D → E: Increase of activity. 1 Publication1
Mutagenesisi224S → A: Complete loss of activity. 1 Publication1
Mutagenesisi226Q → A: Loss of activity. 1 Publication1
Mutagenesisi459W → A: Increase of activity, especially the ATP hydrolysis. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000168032.
PharmGKBiPA27800.

Chemistry databases

ChEMBLiCHEMBL5897.

Polymorphism and mutation databases

BioMutaiENTPD3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002099101 – 529Ectonucleoside triphosphate diphosphohydrolase 3Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi92 ↔ 1161 Publication
Glycosylationi149N-linked (GlcNAc...)Sequence analysis1
Glycosylationi238N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi261 ↔ 3081 Publication
Disulfide bondi289 ↔ 3341 Publication
Disulfide bondi347 ↔ 3531 Publication
Glycosylationi381N-linked (GlcNAc...)Sequence analysis1
Glycosylationi392N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi399 ↔ 4221 Publication
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi454N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO75355.
PeptideAtlasiO75355.
PRIDEiO75355.

PTM databases

iPTMnetiO75355.
PhosphoSitePlusiO75355.

Expressioni

Tissue specificityi

Expressed in adult brain, pancreas, spleen and prostate. Moderate or low expression is seen in most tissues. Not expressed in liver and peripheral blood leukocytes.

Gene expression databases

BgeeiENSG00000168032.
CleanExiHS_ENTPD3.
ExpressionAtlasiO75355. baseline and differential.
GenevisibleiO75355. HS.

Organism-specific databases

HPAiHPA068437.
HPA071802.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SYNE4Q8N2053EBI-10187968,EBI-7131783

Protein-protein interaction databases

BioGridi107394. 1 interactor.
IntActiO75355. 2 interactors.
STRINGi9606.ENSP00000301825.

Chemistry databases

BindingDBiO75355.

Structurei

3D structure databases

ProteinModelPortaliO75355.
SMRiO75355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
GeneTreeiENSGT00550000074435.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiO75355.
KOiK01510.
OMAiNPCYPQN.
OrthoDBiEOG091G05FZ.
PhylomeDBiO75355.
TreeFamiTF332859.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75355-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFTVLTRQPC EQAGLKALYR TPTIIALVVL LVSIVVLVSI TVIQIHKQEV
60 70 80 90 100
LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS
110 120 130 140 150
SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE
160 170 180 190 200
TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK
210 220 230 240 250
NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY
260 270 280 290 300
VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM
310 320 330 340 350
GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ
360 370 380 390 400
ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS
410 420 430 440 450
QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK
460 470 480 490 500
EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPPVFVGT LAFFTAAALL
510 520
CLAFLAYLCS ATRRKRHSEH AFDHAVDSD
Length:529
Mass (Da):59,105
Last modified:September 19, 2006 - v2
Checksum:i5043CF0202978B88
GO
Isoform 2 (identifier: O75355-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-529: VGNSSIAWSL...HAFDHAVDSD → E

Note: No experimental confirmation available.
Show »
Length:452
Mass (Da):50,762
Checksum:i28E1A42977EA63DC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07081324I → V.1 PublicationCorresponds to variant rs17852714dbSNPEnsembl.1
Natural variantiVAR_061384264R → Q.Corresponds to variant rs34266806dbSNPEnsembl.1
Natural variantiVAR_027541440E → D.Corresponds to variant rs4470483dbSNPEnsembl.1
Natural variantiVAR_027542496A → V.1 PublicationCorresponds to variant rs1047855dbSNPEnsembl.1
Natural variantiVAR_027543505L → F.Corresponds to variant rs3733167dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054237452 – 529VGNSS…AVDSD → E in isoform 2. 1 PublicationAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039917 mRNA. Translation: AAC39884.1.
AF034840 mRNA. Translation: AAC09236.2.
AK313322 mRNA. Translation: BAG36127.1.
AC104186 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64600.1.
CH471055 Genomic DNA. Translation: EAW64601.1.
BC029869 mRNA. Translation: AAH29869.1.
CCDSiCCDS2691.1. [O75355-1]
CCDS74919.1. [O75355-2]
RefSeqiNP_001239.2. NM_001248.3. [O75355-1]
NP_001278889.1. NM_001291960.1. [O75355-1]
NP_001278890.1. NM_001291961.1. [O75355-2]
UniGeneiHs.441145.

Genome annotation databases

EnsembliENST00000301825; ENSP00000301825; ENSG00000168032. [O75355-1]
ENST00000445129; ENSP00000404671; ENSG00000168032. [O75355-2]
ENST00000456402; ENSP00000401565; ENSG00000168032. [O75355-1]
GeneIDi956.
KEGGihsa:956.
UCSCiuc003ckd.5. human. [O75355-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039917 mRNA. Translation: AAC39884.1.
AF034840 mRNA. Translation: AAC09236.2.
AK313322 mRNA. Translation: BAG36127.1.
AC104186 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64600.1.
CH471055 Genomic DNA. Translation: EAW64601.1.
BC029869 mRNA. Translation: AAH29869.1.
CCDSiCCDS2691.1. [O75355-1]
CCDS74919.1. [O75355-2]
RefSeqiNP_001239.2. NM_001248.3. [O75355-1]
NP_001278889.1. NM_001291960.1. [O75355-1]
NP_001278890.1. NM_001291961.1. [O75355-2]
UniGeneiHs.441145.

3D structure databases

ProteinModelPortaliO75355.
SMRiO75355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107394. 1 interactor.
IntActiO75355. 2 interactors.
STRINGi9606.ENSP00000301825.

Chemistry databases

BindingDBiO75355.
ChEMBLiCHEMBL5897.

PTM databases

iPTMnetiO75355.
PhosphoSitePlusiO75355.

Polymorphism and mutation databases

BioMutaiENTPD3.

Proteomic databases

PaxDbiO75355.
PeptideAtlasiO75355.
PRIDEiO75355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301825; ENSP00000301825; ENSG00000168032. [O75355-1]
ENST00000445129; ENSP00000404671; ENSG00000168032. [O75355-2]
ENST00000456402; ENSP00000401565; ENSG00000168032. [O75355-1]
GeneIDi956.
KEGGihsa:956.
UCSCiuc003ckd.5. human. [O75355-1]

Organism-specific databases

CTDi956.
GeneCardsiENTPD3.
HGNCiHGNC:3365. ENTPD3.
HPAiHPA068437.
HPA071802.
MIMi603161. gene.
neXtProtiNX_O75355.
OpenTargetsiENSG00000168032.
PharmGKBiPA27800.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
GeneTreeiENSGT00550000074435.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiO75355.
KOiK01510.
OMAiNPCYPQN.
OrthoDBiEOG091G05FZ.
PhylomeDBiO75355.
TreeFamiTF332859.

Enzyme and pathway databases

BioCyciZFISH:HS09682-MONOMER.
BRENDAi3.1.3.31. 2681.
ReactomeiR-HSA-8850843. Phosphate bond hydrolysis by NTPDase proteins.

Miscellaneous databases

GeneWikiiENTPD3.
GenomeRNAii956.
PROiO75355.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168032.
CleanExiHS_ENTPD3.
ExpressionAtlasiO75355. baseline and differential.
GenevisibleiO75355. HS.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENTP3_HUMAN
AccessioniPrimary (citable) accession number: O75355
Secondary accession number(s): B2R8D0
, G5E9N0, O60495, Q8N6K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: September 19, 2006
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.