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O75355

- ENTP3_HUMAN

UniProt

O75355 - ENTP3_HUMAN

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Protein

Ectonucleoside triphosphate diphosphohydrolase 3

Gene
ENTPD3, CD39L3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a threefold preference for the hydrolysis of ATP over ADP.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Ca2+ or Mg2+.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei182 – 1821Proton acceptor By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. nucleoside-diphosphatase activity Source: Ensembl
  3. nucleoside-triphosphatase activity Source: Ensembl

GO - Biological processi

  1. nucleoside diphosphate catabolic process Source: Ensembl
  2. nucleoside triphosphate catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 3 (EC:3.6.1.5)
Short name:
NTPDase 3
Alternative name(s):
CD39 antigen-like 3
Ecto-ATP diphosphohydrolase 3
Short name:
Ecto-ATPDase 3
Short name:
Ecto-ATPase 3
Ecto-apyrase 3
HB6
Gene namesi
Name:ENTPD3
Synonyms:CD39L3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:3365. ENTPD3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei23 – 4321Helical; Reviewed predictionAdd
BLAST
Topological domaini44 – 485442Extracellular Reviewed predictionAdd
BLAST
Transmembranei486 – 50621Helical; Reviewed predictionAdd
BLAST
Topological domaini507 – 52923Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671R → G: Increase of activity. 1 Publication
Mutagenesisi143 – 1431R → A: Loss of activity. 1 Publication
Mutagenesisi143 – 1431R → K: Increase of activity. 1 Publication
Mutagenesisi146 – 1461R → N: No effect. 1 Publication
Mutagenesisi146 – 1461R → P: Increase of ATPase activity, decrease of ADPase activity. 1 Publication
Mutagenesisi146 – 1461R → T: Increase of activity. 1 Publication
Mutagenesisi182 – 1821E → D: Complete loss of activity. 1 Publication
Mutagenesisi182 – 1821E → Q: Complete loss of activity. 1 Publication
Mutagenesisi187 – 1871W → A: Complete loss of activity. 1 Publication
Mutagenesisi191 – 1911N → A: Loss of ATPase activity, increase of ADPase activity. 1 Publication
Mutagenesisi219 – 2191D → E: Increase of activity. 1 Publication
Mutagenesisi224 – 2241S → A: Complete loss of activity. 1 Publication
Mutagenesisi226 – 2261Q → A: Loss of activity. 1 Publication
Mutagenesisi459 – 4591W → A: Increase of activity, especially the ATP hydrolysis. 1 Publication

Organism-specific databases

PharmGKBiPA27800.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 529529Ectonucleoside triphosphate diphosphohydrolase 3PRO_0000209910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi81 – 811N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi92 ↔ 1161 Publication
Glycosylationi149 – 1491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi238 – 2381N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi261 ↔ 3081 Publication
Disulfide bondi289 ↔ 3341 Publication
Disulfide bondi347 ↔ 3531 Publication
Glycosylationi381 – 3811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi392 – 3921N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi399 ↔ 4221 Publication
Glycosylationi402 – 4021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi454 – 4541N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO75355.
PRIDEiO75355.

PTM databases

PhosphoSiteiO75355.

Expressioni

Tissue specificityi

Expressed in adult brain, pancreas, spleen and prostate. Moderate or low expression is seen in most tissues. Not expressed in liver and peripheral blood leukocytes.

Gene expression databases

ArrayExpressiO75355.
BgeeiO75355.
CleanExiHS_ENTPD3.
GenevestigatoriO75355.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000301825.

Structurei

3D structure databases

ProteinModelPortaliO75355.
SMRiO75355. Positions 55-469.

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5371.
HOGENOMiHOG000059572.
HOVERGENiHBG018982.
InParanoidiO75355.
KOiK01510.
OMAiIMQVSLY.
OrthoDBiEOG754HPX.
PhylomeDBiO75355.
TreeFamiTF332859.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 1 hit.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O75355-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MFTVLTRQPC EQAGLKALYR TPTIIALVVL LVSIVVLVSI TVIQIHKQEV    50
LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS 100
SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE 150
TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK 200
NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY 250
VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM 300
GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ 350
ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS 400
QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK 450
EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPPVFVGT LAFFTAAALL 500
CLAFLAYLCS ATRRKRHSEH AFDHAVDSD 529
Length:529
Mass (Da):59,105
Last modified:September 19, 2006 - v2
Checksum:i5043CF0202978B88
GO
Isoform 2 (identifier: O75355-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     452-529: VGNSSIAWSL...HAFDHAVDSD → E

Note: No experimental confirmation available.

Show »
Length:452
Mass (Da):50,762
Checksum:i28E1A42977EA63DC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241I → V.1 Publication
Corresponds to variant rs17852714 [ dbSNP | Ensembl ].
VAR_070813
Natural varianti264 – 2641R → Q.
Corresponds to variant rs34266806 [ dbSNP | Ensembl ].
VAR_061384
Natural varianti440 – 4401E → D.
Corresponds to variant rs4470483 [ dbSNP | Ensembl ].
VAR_027541
Natural varianti496 – 4961A → V.1 Publication
Corresponds to variant rs1047855 [ dbSNP | Ensembl ].
VAR_027542
Natural varianti505 – 5051L → F.
Corresponds to variant rs3733167 [ dbSNP | Ensembl ].
VAR_027543

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei452 – 52978VGNSS…AVDSD → E in isoform 2. VSP_054237Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039917 mRNA. Translation: AAC39884.1.
AF034840 mRNA. Translation: AAC09236.2.
AK313322 mRNA. Translation: BAG36127.1.
AC104186 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64600.1.
CH471055 Genomic DNA. Translation: EAW64601.1.
BC029869 mRNA. Translation: AAH29869.1.
CCDSiCCDS2691.1. [O75355-1]
RefSeqiNP_001239.2. NM_001248.3. [O75355-1]
UniGeneiHs.441145.

Genome annotation databases

EnsembliENST00000301825; ENSP00000301825; ENSG00000168032.
ENST00000445129; ENSP00000404671; ENSG00000168032.
ENST00000456402; ENSP00000401565; ENSG00000168032.
GeneIDi956.
KEGGihsa:956.
UCSCiuc003ckd.4. human. [O75355-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039917 mRNA. Translation: AAC39884.1 .
AF034840 mRNA. Translation: AAC09236.2 .
AK313322 mRNA. Translation: BAG36127.1 .
AC104186 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64600.1 .
CH471055 Genomic DNA. Translation: EAW64601.1 .
BC029869 mRNA. Translation: AAH29869.1 .
CCDSi CCDS2691.1. [O75355-1 ]
RefSeqi NP_001239.2. NM_001248.3. [O75355-1 ]
UniGenei Hs.441145.

3D structure databases

ProteinModelPortali O75355.
SMRi O75355. Positions 55-469.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000301825.

Chemistry

BindingDBi O75355.
ChEMBLi CHEMBL5897.

PTM databases

PhosphoSitei O75355.

Proteomic databases

PaxDbi O75355.
PRIDEi O75355.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000301825 ; ENSP00000301825 ; ENSG00000168032 .
ENST00000445129 ; ENSP00000404671 ; ENSG00000168032 .
ENST00000456402 ; ENSP00000401565 ; ENSG00000168032 .
GeneIDi 956.
KEGGi hsa:956.
UCSCi uc003ckd.4. human. [O75355-1 ]

Organism-specific databases

CTDi 956.
GeneCardsi GC03P040403.
HGNCi HGNC:3365. ENTPD3.
MIMi 603161. gene.
neXtProti NX_O75355.
PharmGKBi PA27800.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5371.
HOGENOMi HOG000059572.
HOVERGENi HBG018982.
InParanoidi O75355.
KOi K01510.
OMAi IMQVSLY.
OrthoDBi EOG754HPX.
PhylomeDBi O75355.
TreeFami TF332859.

Miscellaneous databases

GeneWikii ENTPD3.
GenomeRNAii 956.
NextBioi 35518201.
PROi O75355.
SOURCEi Search...

Gene expression databases

ArrayExpressi O75355.
Bgeei O75355.
CleanExi HS_ENTPD3.
Genevestigatori O75355.

Family and domain databases

InterProi IPR000407. GDA1_CD39_NTPase.
[Graphical view ]
PANTHERi PTHR11782. PTHR11782. 1 hit.
Pfami PF01150. GDA1_CD39. 1 hit.
[Graphical view ]
PROSITEi PS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CD39-like gene family: identification of three new human members (CD39L2, CD39L3, and CD39L4), their murine homologues, and a member of the gene family from Drosophila melanogaster."
    Chadwick B.P., Frischauf A.-M.
    Genomics 50:357-367(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT VAL-496.
    Tissue: Keratinocyte.
  2. "Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases."
    Smith T.M., Kirley T.L.
    Biochim. Biophys. Acta 1386:65-78(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
    Tissue: Brain.
  3. Smith T.M., Kirley T.L.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Esophagus.
  5. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT VAL-24.
    Tissue: Brain.
  8. "Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase."
    Smith T.M., Lewis Carl S.A., Kirley T.L.
    Biochemistry 38:5849-5857(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TRP-187; ASP-219 AND TRP-459.
  9. "Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions."
    Yang F., Hicks-Berger C.A., Smith T.M., Kirley T.L.
    Biochemistry 40:3943-3950(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-67; ARG-143; ARG-146; GLU-182; ASN-191; SER-224 AND GLN-226.
  10. "Characterization of disulfide bonds in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): implications for NTPDase structural modeling."
    Ivanenkov V.V., Meller J., Kirley T.L.
    Biochemistry 44:8998-9012(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiENTP3_HUMAN
AccessioniPrimary (citable) accession number: O75355
Secondary accession number(s): B2R8D0
, G5E9N0, O60495, Q8N6K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: September 19, 2006
Last modified: September 3, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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