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Protein

Guanylate cyclase soluble subunit beta-2

Gene

GUCY1B2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Cofactori

hemeBy similarityNote: Binds 1 or 2 heme groups per heterodimer.By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Iron (heme proximal ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2. 2681.
ReactomeiR-HSA-392154. Nitric oxide stimulates guanylate cyclase.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit beta-2 (EC:4.6.1.2)
Short name:
GCS-beta-2
Gene namesi
Name:GUCY1B2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:4686. GUCY1B2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2111348.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 617617Guanylate cyclase soluble subunit beta-2PRO_0000074119Add
BLAST

Proteomic databases

PeptideAtlasiO75343.
PRIDEiO75343.

PTM databases

iPTMnetiO75343.
PhosphoSiteiO75343.

Expressioni

Gene expression databases

CleanExiHS_GUCY1B2.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Structurei

3D structure databases

ProteinModelPortaliO75343.
SMRiO75343. Positions 3-77, 319-377, 384-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini391 – 519129Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG051715.
InParanoidiO75343.
PhylomeDBiO75343.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGYDRMLRT LGGNLMEFIE NLDALHSYLA LSYQEMNAPS FRVERGADGK
60 70 80 90 100
MFLHYYSDRS GLCHIVPGII EAVAKDFFDI DVIMDILDMN EEVERTGKKE
110 120 130 140 150
HVVFLIVQKA HRKMRKTKPK RLQDSQGMER DQEALQAAFL KMKEKYLNVS
160 170 180 190 200
ACPVKKSHWD VVRSIVMFGK GHLMNTFEPI YPERLWIEEK TFCNAFPFHI
210 220 230 240 250
VFDESLQVKQ ARVNIQKYVP GLQTQNIQLD EYFSIIHPQV TFNIFSIRRF
260 270 280 290 300
INSQFVLKTR REMMPVAWQS RTTLKLQGQM IWMESMWCMV YLCSPKLRSL
310 320 330 340 350
QELEELNMHL SDIAPNDTTR DLILLNQQRL AEIELSNQLE RKKEELQVLS
360 370 380 390 400
KHLAIEKKKT ETLLYAMLPK HVANQLREGK KVAAGEFKSC TILFSDVVTF
410 420 430 440 450
TNICTACEPI QIVNVLNSMY SKFDRLTSVH AVYKVETIGD AYMVVGGVPV
460 470 480 490 500
PIGNHAQRVA NFALGMRISA KEVTNPVTGE PIQLRVGIHT GPVLADVVGD
510 520 530 540 550
KMPRYCLFGD TVNTASRMES HGLPNKVHLS PTAYRALKNQ GFKIIERGEI
560 570 580 590 600
EVKGKGRMTT YFLIQNLNAT EDEIMGRSKT PVDHKGSTQK ASLPTTKLQG
610
SVQPSCPEHS SLASWLL
Length:617
Mass (Da):70,368
Last modified:July 11, 2001 - v2
Checksum:i1B6C965CAB9A5E3E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551Y → C.
Corresponds to variant rs9568497 [ dbSNP | Ensembl ].
VAR_024469
Natural varianti128 – 1281M → I.
Corresponds to variant rs11841997 [ dbSNP | Ensembl ].
VAR_024470
Natural varianti316 – 3161N → H.
Corresponds to variant rs1328361 [ dbSNP | Ensembl ].
VAR_022131

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038499 mRNA. Translation: AAD09440.2.
AF218383 Genomic DNA. Translation: AAF66105.1.
UniGeneiHs.411573.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038499 mRNA. Translation: AAD09440.2.
AF218383 Genomic DNA. Translation: AAF66105.1.
UniGeneiHs.411573.

3D structure databases

ProteinModelPortaliO75343.
SMRiO75343. Positions 3-77, 319-377, 384-563.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2111348.

PTM databases

iPTMnetiO75343.
PhosphoSiteiO75343.

Proteomic databases

PeptideAtlasiO75343.
PRIDEiO75343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GeneCardsiGUCY1B2.
H-InvDBHIX0037298.
HGNCiHGNC:4686. GUCY1B2.
MIMi603695. gene.
neXtProtiNX_O75343.
GenAtlasiSearch...

Phylogenomic databases

HOVERGENiHBG051715.
InParanoidiO75343.
PhylomeDBiO75343.

Enzyme and pathway databases

BRENDAi4.6.1.2. 2681.
ReactomeiR-HSA-392154. Nitric oxide stimulates guanylate cyclase.

Miscellaneous databases

PROiO75343.
SOURCEiSearch...

Gene expression databases

CleanExiHS_GUCY1B2.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCYB2_HUMAN
AccessioniPrimary (citable) accession number: O75343
Secondary accession number(s): Q9NZ64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 11, 2001
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.