O75335 (LIPA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Liprin-alpha-4

Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-4
Short name=PTPRF-interacting protein alpha-4

Gene names

Name:	PPFIA4
Synonyms:	KIAA0897

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates By similarity.
Subunit structure	Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Binds RIMS1, RIMS2, GIT1, KIF1A and GRIP1 By similarity.
Subcellular location	Cytoplasm By similarity. Cell surface By similarity. Note: Colocalizes with PTPRF at the cell surface By similarity.
Tissue specificity	Expressed only in the heart, brain, and skeletal muscle. Ref.4
Domain	The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS By similarity.
Sequence similarities	Belongs to the liprin family. Liprin-alpha subfamily. Contains 3 SAM (sterile alpha motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell communication Non-traceable author statement. Source: UniProtKB
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75335-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75335-2)

The sequence of this isoform differs from the canonical sequence as follows:
     444-452: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Liprin-alpha-4		PRO_0000191032
Regions
	Domain	345 – 411	67	SAM 1
	Domain	460 – 524	65	SAM 2
	Domain	548 – 617	70	SAM 3
	Coiled coil	82 – 128	47	Potential
	Coiled coil	518 – 547	30	Potential
Natural variations
	Alternative sequence	444 – 452	9	Missing in isoform 2.		VSP_009393
Experimental info
	Sequence conflict	650	1	G → A in AAC26102. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 16, 2004. Version 2. Checksum: C35D54A8719A16FC	FASTA	701	78,078
	10 20 30 40 50 60 MGSAADVRFS LGTTTHAPPG VHRRYSALRE ESAKDWETSP LPGMLAPAAG PAFDSDPEIS 70 80 90 100 110 120 DVDEDEPGGL VGSADVVSPS GHSDAQTLAM MLQEQLDAIN EEIRMIQEEK ESTELRAEEI 130 140 150 160 170 180 ETRVTSGSME ALNLKQLRKR GSIPTSLTAL SLASASPPLS GRSTPKLTSR SAAQDLDRMG 190 200 210 220 230 240 VMTLPSDLRK HRRKLLSPVS REENREDKAT IKCETSPPSS PRTLRLEKLG HPALSQEEGK 250 260 270 280 290 300 SALEDQGSNP SSSNSSQDSL HKGAKRKGIK SSIGRLFGKK EKGRLIQLSR DGATGHVLLT 310 320 330 340 350 360 DSEFSMQEPM VPAKLGTQAE KDRRLKKKHQ LLEDARRKGM PFAQWDGPTV VSWLELWVGM 370 380 390 400 410 420 PAWYVAACRA NVKSGAIMSA LSDTEIQREI GISNALHRLK LRLAIQEMVS LTSPSAPPTS 430 440 450 460 470 480 RTSSGNVWVT HEEMETLETS TKTDSEEGSW AQTLAYGDMN HEWIGNEWLP SLGLPQYRSY 490 500 510 520 530 540 FMECLVDARM LDHLTKKDLR VHLKMVDSFH RTSLQYGIMC LKRLNYDRKE LEKRREESQH 550 560 570 580 590 600 EIKDVLVWTN DQVVHWVQSI GLRDYAGNLH ESGVHGALLA LDENFDHNTL ALILQIPTQN 610 620 630 640 650 660 TQARQVMERE FNNLLALGTD RKLDDGDDKV FRRAPSWRKR FRPREHHGRG GMLSASAETL 670 680 690 700 PAGFRVSTLG TLQPPPAPPK KIMPEAHSHY LYGHMLSAFR D
	Isoform 2 [UniParc]. Checksum: 06995B02FADD56B4	FASTA	692	77,088
	10 20 30 40 50 60 MGSAADVRFS LGTTTHAPPG VHRRYSALRE ESAKDWETSP LPGMLAPAAG PAFDSDPEIS 70 80 90 100 110 120 DVDEDEPGGL VGSADVVSPS GHSDAQTLAM MLQEQLDAIN EEIRMIQEEK ESTELRAEEI 130 140 150 160 170 180 ETRVTSGSME ALNLKQLRKR GSIPTSLTAL SLASASPPLS GRSTPKLTSR SAAQDLDRMG 190 200 210 220 230 240 VMTLPSDLRK HRRKLLSPVS REENREDKAT IKCETSPPSS PRTLRLEKLG HPALSQEEGK 250 260 270 280 290 300 SALEDQGSNP SSSNSSQDSL HKGAKRKGIK SSIGRLFGKK EKGRLIQLSR DGATGHVLLT 310 320 330 340 350 360 DSEFSMQEPM VPAKLGTQAE KDRRLKKKHQ LLEDARRKGM PFAQWDGPTV VSWLELWVGM 370 380 390 400 410 420 PAWYVAACRA NVKSGAIMSA LSDTEIQREI GISNALHRLK LRLAIQEMVS LTSPSAPPTS 430 440 450 460 470 480 RTSSGNVWVT HEEMETLETS TKTTLAYGDM NHEWIGNEWL PSLGLPQYRS YFMECLVDAR 490 500 510 520 530 540 MLDHLTKKDL RVHLKMVDSF HRTSLQYGIM CLKRLNYDRK ELEKRREESQ HEIKDVLVWT 550 560 570 580 590 600 NDQVVHWVQS IGLRDYAGNL HESGVHGALL ALDENFDHNT LALILQIPTQ NTQARQVMER 610 620 630 640 650 660 EFNNLLALGT DRKLDDGDDK VFRRAPSWRK RFRPREHHGR GGMLSASAET LPAGFRVSTL 670 680 690 GTLQPPPAPP KKIMPEAHSH YLYGHMLSAF RD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cerebellum.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins." Serra-Pages C., Medley Q.G., Tang M., Hart A., Streuli M. J. Biol. Chem. 273:15611-15620(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 142-650 (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Brain, Fetal brain, Heart and Kidney.
[5]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 283-701 (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK126624 mRNA. Translation: BAC86617.1. AL451082, AC105940 Genomic DNA. Translation: CAH72275.2. BC132923 mRNA. Translation: AAI32924.1. BC132925 mRNA. Translation: AAI32926.1. AF034801 mRNA. Translation: AAC26102.1. AB020704 mRNA. Translation: BAA74920.1.
IPI	IPI00397579. IPI00916144.
RefSeq	NP_055868.1. NM_015053.1.
UniGene	Hs.153648.
3D structure databases
ProteinModelPortal	O75335.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75335. 2 interactions.
MINT	MINT-2865217.
STRING	9606.ENSP00000272198.
PTM databases
PhosphoSite	O75335.
Proteomic databases
PaxDb	O75335.
PRIDE	O75335.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000272198; ENSP00000272198; ENSG00000143847. ENST00000295706; ENSP00000295706; ENSG00000143847. ENST00000599966; ENSP00000471264; ENSG00000143847.
GeneID	8497.
KEGG	hsa:8497.
UCSC	uc001gyz.3. human. uc001gza.3. human.
Organism-specific databases
CTD	8497.
GeneCards	GC01P202995.
HGNC	HGNC:9248. PPFIA4.
MIM	603145. gene.
neXtProt	NX_O75335.
PharmGKB	PA33569.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG322697.
HOGENOM	HOG000017513.
HOVERGEN	HBG052330.
OrthoDB	EOG4PC9RB.
PhylomeDB	O75335.
Gene expression databases
ArrayExpress	O75335.
Bgee	O75335.
CleanEx	HS_PPFIA4.
Genevestigator	O75335.
GermOnline	ENSG00000143847. Homo sapiens.
Family and domain databases
Gene3D	1.10.150.50. 3 hits.
InterPro	IPR001660. SAM. IPR013761. SAM/pointed. IPR011510. SAM_2. IPR021129. SAM_type1. [Graphical view]
Pfam	PF00536. SAM_1. 2 hits. PF07647. SAM_2. 1 hit. [Graphical view]
SMART	SM00454. SAM. 3 hits. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 3 hits.
PROSITE	PS50105. SAM_DOMAIN. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PPFIA4. human.
GenomeRNAi	8497.
NextBio	31789.
SOURCE	Search...

Entry information

Entry name

LIPA4_HUMAN

Accession

Primary (citable) accession number: O75335
Secondary accession number(s): A2RUJ5, B1APN8, O94971

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.