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O75335 (LIPA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Liprin-alpha-4
Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-4
Short name=PTPRF-interacting protein alpha-4
Gene names
Name:PPFIA4
Synonyms:KIAA0897
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1185 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates By similarity.

Subunit structure

Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Binds RIMS1, RIMS2, GIT1, KIF1A and GRIP1 By similarity.

Subcellular location

Cytoplasm By similarity. Cell surface By similarity. Note: Colocalizes with PTPRF at the cell surface By similarity.

Tissue specificity

Expressed only in the heart, brain, and skeletal muscle. Ref.5

Domain

The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS By similarity.

Sequence similarities

Belongs to the liprin family. Liprin-alpha subfamily.

Contains 3 SAM (sterile alpha motif) domains.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcell surface

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

intracellular

Traceable author statement Ref.5. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 12923177. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 3 (identifier: O75335-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: O75335-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-510: Missing.
     545-588: LALPLTVTLR...GSSGPLPVPE → DWETSPLPGM...QLDAINEEIR
Isoform 2 (identifier: O75335-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-510: Missing.
     545-588: LALPLTVTLR...GSSGPLPVPE → DWETSPLPGM...QLDAINEEIR
     928-936: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11851185Liprin-alpha-4
PRO_0000191032

Regions

Domain829 – 89567SAM 1
Domain944 – 100865SAM 2
Domain1032 – 110170SAM 3
Coiled coil24 – 123100 Potential
Coiled coil165 – 499335 Potential

Natural variations

Alternative sequence1 – 510510Missing in isoform 1 and isoform 2.
VSP_053668
Alternative sequence545 – 58844LALPL…LPVPE → DWETSPLPGMLAPAAGPAFD SDPEISDVDEDEPGGLVGSA DVVSPSGHSDAQTLAMMLQE QLDAINEEIR in isoform 1 and isoform 2.
VSP_053669
Alternative sequence928 – 9369Missing in isoform 2.
VSP_053670

Experimental info

Sequence conflict8031A → S in AAI44263. Ref.3
Sequence conflict11341G → A in AAC26102. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 3 [UniParc].

Last modified February 19, 2014. Version 3.
Checksum: 0DD1431820D2313E

FASTA1,185134,404
        10         20         30         40         50         60 
MCEVMPTINE GDRLGPPHGA DADANFEQLM VNMLDEREKL LESLRESQET LAATQSRLQD 

        70         80         90        100        110        120 
AIHERDQLQR HLNSALPQEF ATLTRELSMC REQLLEREEE ISELKAERNN TRLLLEHLEC 

       130        140        150        160        170        180 
LVSRHERSLR MTVVKRQAQS PSGVSSEVEV LKALKSLFEH HKALDEKVRE RLRAALERVT 

       190        200        210        220        230        240 
TLEEQLAGAH QQVSALQQGA GVRDGAAEEE GTVELGPKRL WKEDTGRVEE LQELLEKQNF 

       250        260        270        280        290        300 
ELSQARERLV TLTTTVTELE EDLGTARRDL IKSEELSSKH QRDLREALAQ KEDMEERITT 

       310        320        330        340        350        360 
LEKRYLAAQR EATSIHDLND KLENELANKE SLHRQCEEKA RHLQELLEVA EQKLQQTMRK 

       370        380        390        400        410        420 
AETLPEVEAE LAQRIAALTK AEERHGNIEE HLRQLEGQLE EKNQELARTA VQVRQREKMN 

       430        440        450        460        470        480 
EDHNKRLSDT VDRLLSESNE RLQLHLKERM AALEEKNTLI QELESSQRQI EEQHHHKGRL 

       490        500        510        520        530        540 
SEEIEKLRQE VDQLKGRGGP FVDGVHSRSH MGSAADVRFS LGTTTHAPPG VHRRYSALRE 

       550        560        570        580        590        600 
ESAKLALPLT VTLRSPTWMR MSQGVCCNLE YHSSGTLCGS SGPLPVPEMI QEEKESTELR 

       610        620        630        640        650        660 
AEEIETRVTS GSMEALNLKQ LRKRGSIPTS LTALSLASAS PPLSGRSTPK LTSRSAAQDL 

       670        680        690        700        710        720 
DRMGVMTLPS DLRKHRRKLL SPVSREENRE DKATIKCETS PPSSPRTLRL EKLGHPALSQ 

       730        740        750        760        770        780 
EEGKSALEDQ GSNPSSSNSS QDSLHKGAKR KGIKSSIGRL FGKKEKGRLI QLSRDGATGH 

       790        800        810        820        830        840 
VLLTDSEFSM QEPMVPAKLG TQAEKDRRLK KKHQLLEDAR RKGMPFAQWD GPTVVSWLEL 

       850        860        870        880        890        900 
WVGMPAWYVA ACRANVKSGA IMSALSDTEI QREIGISNAL HRLKLRLAIQ EMVSLTSPSA 

       910        920        930        940        950        960 
PPTSRTSSGN VWVTHEEMET LETSTKTDSE EGSWAQTLAY GDMNHEWIGN EWLPSLGLPQ 

       970        980        990       1000       1010       1020 
YRSYFMECLV DARMLDHLTK KDLRVHLKMV DSFHRTSLQY GIMCLKRLNY DRKELEKRRE 

      1030       1040       1050       1060       1070       1080 
ESQHEIKDVL VWTNDQVVHW VQSIGLRDYA GNLHESGVHG ALLALDENFD HNTLALILQI 

      1090       1100       1110       1120       1130       1140 
PTQNTQARQV MEREFNNLLA LGTDRKLDDG DDKVFRRAPS WRKRFRPREH HGRGGMLSAS 

      1150       1160       1170       1180 
AETLPAGFRV STLGTLQPPP APPKKIMPEA HSHYLYGHML SAFRD 

« Hide

Isoform 1 [UniParc].

Checksum: C35D54A8719A16FC
Show »

FASTA70178,078
Isoform 2 [UniParc].

Checksum: 06995B02FADD56B4
Show »

FASTA69277,088

References

« Hide 'large scale' references
[1]"Characterization of liprins in the central nervous system."
Spangler S.A., Hoogenraad C.C.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Cerebellum.
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[5]"Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins."
Serra-Pages C., Medley Q.G., Tang M., Hart A., Streuli M.
J. Biol. Chem. 273:15611-15620(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 626-1134 (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Brain, Fetal brain, Heart and Kidney.
[6]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 767-1185 (ISOFORM 1).
Tissue: Brain.
[7]"Identification and characterization of human PPFIA4 gene in silico."
Katoh M., Katoh M.
Int. J. Mol. Med. 12:1009-1014(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF428334 mRNA. Translation: ABR09268.1.
AK126624 mRNA. Translation: BAC86617.1.
AC096632 Genomic DNA. No translation available.
AL451082, AC105940 Genomic DNA. Translation: CAH72275.2.
BC132923 mRNA. Translation: AAI32924.1.
BC132925 mRNA. Translation: AAI32926.1.
BC144262 mRNA. Translation: AAI44263.1.
AF034801 mRNA. Translation: AAC26102.1.
AB020704 mRNA. Translation: BAA74920.1.
CCDSCCDS44296.1. [O75335-1]
RefSeqNP_055868.1. NM_015053.1. [O75335-1]
UniGeneHs.153648.

3D structure databases

ProteinModelPortalO75335.
SMRO75335. Positions 802-1106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114069. 5 interactions.
IntActO75335. 2 interactions.
MINTMINT-2865217.
STRING9606.ENSP00000272198.

PTM databases

PhosphoSiteO75335.

Proteomic databases

MaxQBO75335.
PaxDbO75335.
PRIDEO75335.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000272198; ENSP00000272198; ENSG00000143847. [O75335-1]
ENST00000295706; ENSP00000295706; ENSG00000143847. [O75335-2]
ENST00000447715; ENSP00000402576; ENSG00000143847. [O75335-3]
ENST00000599966; ENSP00000471264; ENSG00000143847. [O75335-2]
GeneID8497.
KEGGhsa:8497.
UCSCuc001gyz.3. human. [O75335-3]
uc001gza.3. human. [O75335-2]
uc009xaj.3. human.

Organism-specific databases

CTD8497.
GeneCardsGC01P202995.
HGNCHGNC:9248. PPFIA4.
HPAHPA053419.
HPA054132.
MIM603145. gene.
neXtProtNX_O75335.
PharmGKBPA33569.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG322697.
HOGENOMHOG000017513.
HOVERGENHBG052330.
TreeFamTF314207.

Gene expression databases

ArrayExpressO75335.
BgeeO75335.
CleanExHS_PPFIA4.
GenevestigatorO75335.

Family and domain databases

Gene3D1.10.150.50. 3 hits.
InterProIPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR021129. SAM_type1.
[Graphical view]
PfamPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMSSF47769. SSF47769. 3 hits.
PROSITEPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPPFIA4. human.
GeneWikiPPFIA4.
GenomeRNAi8497.
NextBio31789.
PROO75335.
SOURCESearch...

Entry information

Entry nameLIPA4_HUMAN
AccessionPrimary (citable) accession number: O75335
Secondary accession number(s): A2RUJ5 expand/collapse secondary AC list , B1APN8, B1N949, B7ZM43, O94971
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 19, 2014
Last modified: July 9, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM