O75334 (LIPA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Liprin-alpha-2

Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-2
Short name=PTPRF-interacting protein alpha-2

Gene names

Name:

PPFIA2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1257 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. Ref.1
Subunit structure	Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Ref.1
Subcellular location	Cytoplasm. Cell surface. Note: Colocalizes with PTPRF at the cell surface. Ref.1
Tissue specificity	Expressed only in brain. Ref.1
Domain	The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS.
Sequence similarities	Belongs to the liprin family. Liprin-alpha subfamily. Contains 3 SAM (sterile alpha motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell-matrix adhesion Traceable author statement. Source: ProtInc
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1257	1257	Liprin-alpha-2		PRO_0000191027
Regions
	Domain	898 – 964	67	SAM 1
	Domain	1020 – 1084	65	SAM 2
	Domain	1108 – 1177	70	SAM 3
	Coiled coil	29 – 154	126	Potential
	Coiled coil	185 – 541	357	Potential
	Coiled coil	643 – 695	53	Potential
	Coiled coil	1081 – 1107	27	Potential
	Compositional bias	115 – 528	414	Glu-rich
	Compositional bias	622 – 627	6	Poly-Asp
Amino acid modifications
	Modified residue	538	1	Phosphoserine By similarity
	Modified residue	689	1	Phosphoserine By similarity
	Modified residue	774	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O75334 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: 772C0213BA1AFE21	FASTA	1,257	143,142
	10 20 30 40 50 60 MMCEVMPTIN EDTPMSQRGS QSSGSDSDSH FEQLMVNMLD ERDRLLDTLR ETQESLSLAQ 70 80 90 100 110 120 QRLQDVIYDR DSLQRQLNSA LPQDIESLTG GLAGSKGADP PEFAALTKEL NACREQLLEK 130 140 150 160 170 180 EEEISELKAE RNNTRLLLEH LECLVSRHER SLRMTVVKRQ AQSPSGVSSE VEVLKALKSL 190 200 210 220 230 240 FEHHKALDEK VRERLRVSLE RVSALEEELA AANQEIVALR EQNVHIQRKM ASSEGSTESE 250 260 270 280 290 300 HLEGMEPGQK VHEKRLSNGS IDSTDETSQI VELQELLEKQ NYEMAQMKER LAALSSRVGE 310 320 330 340 350 360 VEQEAETARK DLIKTEEMNT KYQRDIREAM AQKEDMEERI TTLEKRYLSA QRESTSIHDM 370 380 390 400 410 420 NDKLENELAN KEAILRQMEE KNRQLQERLE LAEEKLQQTM RKAETLPEVE AELAQRIAAL 430 440 450 460 470 480 TKAEETHGNI EERMRHLEGQ LEEKNQELQR ARQREKMNEE HNKRLSDTVD RLLTESNERL 490 500 510 520 530 540 QLHLKERMAA LEEKNVLIQE SETFRKNLEE SLHDKESLAE EIEKLRSELD QLKMRTGSLI 550 560 570 580 590 600 EPTIPRTHLD TSAELRYSVG SLVDSQSDYR TTKVIRRPRR GRMGVRRDEP KVKSLGDHEW 610 620 630 640 650 660 NRTQQIGVLS SHPFESDTEM SDIDDDDRET IFSSMDLLSP SGHSDAQTLA MMLQEQLDAI 670 680 690 700 710 720 NKEIRLIQEE KESTELRAEE IENRVASVSL EGLNLAMVHP GTSITASVTA SSLASSSPPS 730 740 750 760 770 780 GHSTPKLTPR SPAREMDRMG VMTLPSDLRK HRRKIAVVEE DGREDKATIK CETSPPPTPR 790 800 810 820 830 840 ALRMTHTLPS SYHNDARSSL SVSLEPESLG LGSANSSQDS LHKAPKKKGI KSSIGRLFGK 850 860 870 880 890 900 KEKARLGQLR GFMETEAAAQ ESLGLGKLGT QAEKDRRLKK KHELLEEARR KGLPFAQWDG 910 920 930 940 950 960 PTVVAWLELW LGMPAWYVAA CRANVKSGAI MSALSDTEIQ REIGISNPLH RLKLRLAIQE 970 980 990 1000 1010 1020 MVSLTSPSAP PTSRTPSGNV WVTHEEMENL AAPAKTKESE EGSWAQCPVF LQTLAYGDMN 1030 1040 1050 1060 1070 1080 HEWIGNEWLP SLGLPQYRSY FMECLVDARM LDHLTKKDLR VHLKMVDSFH RTSLQYGIMC 1090 1100 1110 1120 1130 1140 LKRLNYDRKE LERRREASQH EIKDVLVWSN DRVIRWIQAI GLREYANNIL ESGVHGSLIA 1150 1160 1170 1180 1190 1200 LDENFDYSSL ALLLQIPTQN TQARQILERE YNNLLALGTE RRLDESDDKN FRRGSTWRRQ 1210 1220 1230 1240 1250 FPPREVHGIS MMPGSSETLP AGFRLTTTSG QSRKMTTDVA SSRLQRLDNS TVRTYSC

References

[1]	"Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins." Serra-Pages C., Medley Q.G., Tang M., Hart A., Streuli M. J. Biol. Chem. 273:15611-15620(1998) [PubMed: 9624153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PTPRD; PTPRF AND PTPRS. Tissue: Brain, Fetal brain, Heart and Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF034799 mRNA. Translation: AAC26100.1.

IPI

IPI00289271.

RefSeq

NP_003616.2. NM_003625.3.

UniGene

Hs.506216.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TAC	X-ray	2.20	B	866-1193	[»]
3TAD	X-ray	2.90	A/B	866-1193	[»]

ProteinModelPortal

O75334.

SMR

O75334. Positions 893-959, 1017-1084.

ModBase

Search...

Protein-protein interaction databases

IntAct

O75334. 3 interactions.

STRING

O75334.

PTM databases

PhosphoSite

O75334.

Proteomic databases

PRIDE

O75334.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000333447; ENSP00000327416; ENSG00000139220.

GeneID

8499.

KEGG

hsa:8499.

Organism-specific databases

CTD

8499.

GeneCards

GC12M081584.

H-InvDB

HIX0010857.

HGNC

HGNC:9246. PPFIA2.

HPA

HPA040724.

MIM

603143. gene.

neXtProt

NX_O75334.

PharmGKB

PA33567.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18893.

HOVERGEN

HBG052330.

InParanoid

O75334.

Gene expression databases

ArrayExpress

O75334.

Bgee

O75334.

CleanEx

HS_PPFIA2.

Genevestigator

O75334.

GermOnline

ENSG00000139220. Homo sapiens.

Family and domain databases

InterPro

IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR021129. SAM_type1.
[Graphical view]

Gene3D

G3DSA:1.10.150.50. SAM_type. 3 hits.

Pfam

PF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]

SMART

SM00454. SAM. 3 hits.
[Graphical view]

SUPFAM

SSF47769. SAM_homology. 3 hits.

PROSITE

PS50105. SAM_DOMAIN. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

31801.

SOURCE

Search...

Entry information

Entry name

LIPA2_HUMAN

Accession

Primary (citable) accession number: O75334

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	November 1, 1998
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.