ID   UBP12_HUMAN             Reviewed;         370 AA.
AC   O75317; A8K0X0; Q5VZV3; Q8TC49;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 2.
DT   07-JUL-2009, entry version 75.
DE   RecName: Full=Ubiquitin carboxyl-terminal hydrolase 12;
DE            EC=3.1.2.15;
DE   AltName: Full=Ubiquitin thioesterase 12;
DE   AltName: Full=Ubiquitin-specific-processing protease 12;
DE   AltName: Full=Deubiquitinating enzyme 12;
DE   AltName: Full=Ubiquitin-hydrolyzing enzyme 1;
GN   Name=USP12; Synonyms=UBH1, USP12L1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=98277453; PubMed=9615226; DOI=10.1006/geno.1998.5275;
RA   Hansen-Hagge T.E., Janssen J.W.G., Hameister H., Papa F.R.,
RA   Zechner U., Seriu T., Jauch A., Becke D., Hochstrasser M.,
RA   Bartram C.R.;
RT   "An evolutionarily conserved gene on human chromosome 5q33-q34, UBH1,
RT   encodes a novel deubiquitinating enzyme.";
RL   Genomics 49:411-418(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Amygdala;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057823; DOI=10.1038/nature02379;
RA   Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA   Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E.,
RA   Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.,
RA   Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R.,
RA   Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S.,
RA   Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA   Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M.,
RA   Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J.,
RA   Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E.,
RA   Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L.,
RA   Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J.,
RA   Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S.,
RA   Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J.,
RA   Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M.,
RA   King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A.,
RA   Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S.,
RA   Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA   Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I.,
RA   Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S.,
RA   Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A.,
RA   Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B.,
RA   Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L.,
RA   Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M.,
RA   Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.;
RT   "The DNA sequence and analysis of human chromosome 13.";
RL   Nature 428:522-528(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY, CATALYTIC ACTIVITY, FUNCTION,
RP   INTERACTION WITH WDR48, AND MUTAGENESIS OF CYS-48.
RX   PubMed=19075014; DOI=10.1074/jbc.M808430200;
RA   Cohn M.A., Kee Y., Haas W., Gygi S.P., D'Andrea A.D.;
RT   "UAF1 is a subunit of multiple deubiquitinating enzyme complexes.";
RL   J. Biol. Chem. 284:5343-5351(2009).
CC   -!- FUNCTION: Deubiquitinating enzyme. Has almost no deubiquitinating
CC       activity by itself and requires the interaction with WDR48 to have
CC       a high activity. Not involved in deubiquitination of
CC       monoubiquitinated FANCD2.
CC   -!- CATALYTIC ACTIVITY: Ubiquitin C-terminal thioester + H(2)O =
CC       ubiquitin + a thiol.
CC   -!- SUBUNIT: Interacts with WDR48.
CC   -!- SIMILARITY: Belongs to the peptidase C19 family. USP12/USP46
CC       subfamily.
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DR   EMBL; AF022789; AAC23551.1; ALT_INIT; mRNA.
DR   EMBL; AK289685; BAF82374.1; -; mRNA.
DR   EMBL; AL158062; CAH70555.1; -; Genomic_DNA.
DR   EMBL; AL355473; CAH70555.1; JOINED; Genomic_DNA.
DR   EMBL; AL355473; CAI16331.1; -; Genomic_DNA.
DR   EMBL; AL158062; CAI16331.1; JOINED; Genomic_DNA.
DR   EMBL; CH471075; EAX08392.1; -; Genomic_DNA.
DR   EMBL; BC026072; AAH26072.1; -; mRNA.
DR   IPI; IPI00290893; -.
DR   RefSeq; NP_872294.1; -.
DR   UniGene; Hs.42400; -.
DR   HSSP; Q93009; 1NBF.
DR   MEROPS; C19.020; -.
DR   MEROPS; C19.062; -.
DR   Ensembl; ENSG00000152484; Homo sapiens.
DR   GeneID; 219333; -.
DR   GeneCards; GC13M026540; -.
DR   H-InvDB; HIX0011190; -.
DR   HGNC; HGNC:20485; USP12.
DR   HPA; HPA007288; -.
DR   MIM; 603091; gene.
DR   PharmGKB; PA27522; -.
DR   HOGENOM; O75317; -.
DR   HOVERGEN; O75317; -.
DR   OMA; O75317; QPRRKEN.
DR   BRENDA; 3.1.2.15; 247.
DR   NextBio; 90574; -.
DR   ArrayExpress; O75317; -.
DR   Bgee; O75317; -.
DR   CleanEx; HS_USP12; -.
DR   GermOnline; ENSG00000152484; Homo sapiens.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IMP:UniProtKB.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0004221; F:ubiquitin thiolesterase activity; IDA:UniProtKB.
DR   GO; GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
DR   GO; GO:0016579; P:protein deubiquitination; IDA:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR   InterPro; IPR018200; Pept_C19ubi-hydrolase_C_CS.
DR   InterPro; IPR001394; Peptidase_C19.
DR   Pfam; PF00443; UCH; 1.
DR   PROSITE; PS00972; UCH_2_1; 1.
DR   PROSITE; PS00973; UCH_2_2; 1.
DR   PROSITE; PS50235; UCH_2_3; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Hydrolase; Protease; Thiol protease;
KW   Ubl conjugation pathway.
FT   CHAIN         1    370       Ubiquitin carboxyl-terminal hydrolase 12.
FT                                /FTId=PRO_0000080634.
FT   ACT_SITE     48     48       Probable.
FT   ACT_SITE    308    308       By similarity.
FT   ACT_SITE    317    317       By similarity.
FT   MUTAGEN      48     48       C->S: Loss of activity.
FT   CONFLICT    173    173       H -> D (in Ref. 5; AAH26072).
SQ   SEQUENCE   370 AA;  42858 MW;  267EE6A3674F6440 CRC64;
     MEILMTVSKF ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC NSVLQALYFC
     RPFREKVLAY KSQPRKKESL LTCLADLFHS IATQKKKVGV IPPKKFITRL RKENELFDNY
     MQQDAHEFLN YLLNTIADIL QEERKQEKQN GRLPNGNIDN ENNNSTPDPT WVHEIFQGTL
     TNETRCLTCE TISSKDEDFL DLSVDVEQNT SITHCLRGFS NTETLCSEYK YYCEECRSKQ
     EAHKRMKVKK LPMILALHLK RFKYMDQLHR YTKLSYRVVF PLELRLFNTS GDATNPDRMY
     DLVAVVVHCG SGPNRGHYIA IVKSHDFWLL FDDDIVEKID AQAIEEFYGL TSDISKNSES
     GYILFYQSRD
//