O75311 (GLRA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine receptor subunit alpha-3

Gene names

Name:

GLRA3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
Subunit structure	Pentamer composed of alpha and beta subunits By similarity.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Tissue specificity	Widely distributed throughout the central nervous system.
Domain	The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.
Miscellaneous	The alpha subunit binds strychnine.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA3 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	synaptic transmission Non-traceable author statement. Source: UniProtKB
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Non-traceable author statement. Source: UniProtKB integral to plasma membrane Inferred from electronic annotation. Source: InterPro plasma membrane Traceable author statement. Source: Reactome postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	extracellular ligand-gated ion channel activity Non-traceable author statement. Source: UniProtKB extracellular-glycine-gated chloride channel activity Inferred from electronic annotation. Source: InterPro glycine binding Inferred from electronic annotation. Source: InterPro transmitter-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha-3L (identifier: O75311-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha-3K (identifier: O75311-2)

The sequence of this isoform differs from the canonical sequence as follows:
     358-372: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 464	431	Glycine receptor subunit alpha-3		PRO_0000000419
Regions
	Topological domain	34 – 252	219	Extracellular Probable
	Transmembrane	253 – 279	27	Helical; Probable
	Transmembrane	286 – 303	18	Helical; Probable
	Transmembrane	315 – 341	27	Helical; Probable
	Topological domain	342 – 433	92	Cytoplasmic Probable
	Transmembrane	434 – 451	18	Helical; Probable
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	231 ↔ 242		By similarity
Natural variations
	Alternative sequence	358 – 372	15	Missing in isoform Alpha-3K.		VSP_000084
Experimental info
	Sequence conflict	460	1	H → HH in AAC39919. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha-3L [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 8E17A30B3C6E648D	FASTA	464	53,800
	10 20 30 40 50 60 MAHVRHFRTL VSGFYFWEAA LLLSLVATKE TDSARSRSAP MSPSDFLDKL MGRTSGYDAR 70 80 90 100 110 120 IRPNFKGPPV NVTCNIFINS FGSIAETTMD YRVNIFLRQK WNDPRLAYSE YPDDSLDLDP 130 140 150 160 170 180 SMLDSIWKPD LFFANEKGAN FHEVTTDNKL LRIFKNGNVL YSIRLTLTLS CPMDLKNFPM 190 200 210 220 230 240 DVQTCIMQLE SFGYTMNDLI FEWQDEAPVQ VAEGLTLPQF LLKEEKDLRY CTKHYNTGKF 250 260 270 280 290 300 TCIEVRFHLE RQMGYYLIQM YIPSLLIVIL SWVSFWINMD AAPARVALGI TTVLTMTTQS 310 320 330 340 350 360 SGSRASLPKV SYVKAIDIWM AVCLLFVFSA LLEYAAVNFV SRQHKELLRF RRKRKNKTEA 370 380 390 400 410 420 FALEKFYRFS DMDDEVRESR FSFTAYGMGP CLQAKDGMTP KGPNHPVQVM PKSPDEMRKV 430 440 450 460 FIDRAKKIDT ISRACFPLAF LIFNIFYWVI YKILRHEDIH QQQD
	Isoform Alpha-3K [UniParc]. Checksum: 342BB384CCA4D33E	FASTA	449	51,963
	10 20 30 40 50 60 MAHVRHFRTL VSGFYFWEAA LLLSLVATKE TDSARSRSAP MSPSDFLDKL MGRTSGYDAR 70 80 90 100 110 120 IRPNFKGPPV NVTCNIFINS FGSIAETTMD YRVNIFLRQK WNDPRLAYSE YPDDSLDLDP 130 140 150 160 170 180 SMLDSIWKPD LFFANEKGAN FHEVTTDNKL LRIFKNGNVL YSIRLTLTLS CPMDLKNFPM 190 200 210 220 230 240 DVQTCIMQLE SFGYTMNDLI FEWQDEAPVQ VAEGLTLPQF LLKEEKDLRY CTKHYNTGKF 250 260 270 280 290 300 TCIEVRFHLE RQMGYYLIQM YIPSLLIVIL SWVSFWINMD AAPARVALGI TTVLTMTTQS 310 320 330 340 350 360 SGSRASLPKV SYVKAIDIWM AVCLLFVFSA LLEYAAVNFV SRQHKELLRF RRKRKNKDDE 370 380 390 400 410 420 VRESRFSFTA YGMGPCLQAK DGMTPKGPNH PVQVMPKSPD EMRKVFIDRA KKIDTISRAC 430 440 FPLAFLIFNI FYWVIYKILR HEDIHQQQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human glycine receptor subunit alpha3. GLRA3 gene structure, chromosomal localization, and functional characterization of alternative transcripts." Nikolic Z., Laube B., Weber R.G., Lichter P., Kioschis P., Poustka A., Muelhardt C., Becker C.-M. J. Biol. Chem. 273:19708-19714(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS ALPHA-3K AND ALPHA-3L). Tissue: Fetal brain.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-3L). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017724 , AF017715, AF017716, AF017717, AF017718, AF017719, AF017720, AF017721, AF017722, AF017723 Genomic DNA. Translation: AAC39919.1. U93917 mRNA. Translation: AAC39917.1. CH471056 Genomic DNA. Translation: EAX04730.1. CH471056 Genomic DNA. Translation: EAX04732.1. BC036086 mRNA. Translation: AAH36086.1.
IPI	IPI00025243. IPI00216193.
RefSeq	NP_001036008.1. NM_001042543.1. NP_006520.2. NM_006529.2.
UniGene	Hs.413099.
3D structure databases
ProteinModelPortal	O75311.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000274093.
Protein family/group databases
TCDB	1.A.9.3.1. neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.
PTM databases
PhosphoSite	O75311.
Proteomic databases
PaxDb	O75311.
PRIDE	O75311.
Protocols and materials databases
DNASU	8001.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274093; ENSP00000274093; ENSG00000145451. ENST00000340217; ENSP00000345284; ENSG00000145451.
GeneID	8001.
KEGG	hsa:8001.
UCSC	uc003ity.1. human. uc003itz.1. human.
Organism-specific databases
CTD	8001.
GeneCards	GC04M175558.
HGNC	HGNC:4328. GLRA3.
HPA	HPA014805.
MIM	600421. gene.
neXtProt	NX_O75311.
PharmGKB	PA28729.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG265706.
HOGENOM	HOG000231336.
HOVERGEN	HBG051707.
InParanoid	O75311.
KO	K05195.
OMA	PKNSDEM.
OrthoDB	EOG4TF0KF.
PhylomeDB	O75311.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	O75311.
Bgee	O75311.
CleanEx	HS_GLRA3.
Genevestigator	O75311.
GermOnline	ENSG00000145451. Homo sapiens.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006028. GABAA_rcpt. IPR008127. Glycine_rcpt_A. IPR008130. Glycine_rcpt_A3. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00253. GABAARECEPTR. PR01673. GLYRALPHA. PR01676. GLYRALPHA3. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O75311.
ChEMBL	CHEMBL1075092.
DrugBank	DB00145. Glycine.
GenomeRNAi	8001.
NextBio	30552.
SOURCE	Search...

Entry information

Entry name

GLRA3_HUMAN

Accession

Primary (citable) accession number: O75311
Secondary accession number(s): D3DP44, O75816, Q5D0E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.