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Protein

Glycine receptor subunit alpha-3

Gene

GLRA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

GO - Molecular functioni

  1. extracellular-glycine-gated chloride channel activity Source: InterPro
  2. glycine binding Source: InterPro
  3. transmitter-gated ion channel activity Source: InterPro

GO - Biological processi

  1. ion transmembrane transport Source: Reactome
  2. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_25387. Ligand-gated ion channel transport.

Protein family/group databases

TCDBi1.A.9.3.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-3
Gene namesi
Name:GLRA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:4328. GLRA3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 252219ExtracellularCuratedAdd
BLAST
Transmembranei253 – 27927HelicalCuratedAdd
BLAST
Transmembranei286 – 30318HelicalCuratedAdd
BLAST
Transmembranei315 – 34127HelicalCuratedAdd
BLAST
Topological domaini342 – 43392CytoplasmicCuratedAdd
BLAST
Transmembranei434 – 45118HelicalCuratedAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chloride channel complex Source: UniProtKB-KW
  3. integral component of plasma membrane Source: InterPro
  4. plasma membrane Source: Reactome
  5. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28729.

Chemistry

DrugBankiDB00145. Glycine.
DB00602. Ivermectin.
DB00431. Lindane.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 464431Glycine receptor subunit alpha-3PRO_0000000419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi171 ↔ 185By similarity
Disulfide bondi231 ↔ 242By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO75311.
PRIDEiO75311.

PTM databases

PhosphoSiteiO75311.

Expressioni

Tissue specificityi

Widely distributed throughout the central nervous system.

Gene expression databases

BgeeiO75311.
CleanExiHS_GLRA3.
ExpressionAtlasiO75311. baseline and differential.
GenevestigatoriO75311.

Organism-specific databases

HPAiHPA014805.

Interactioni

Subunit structurei

Pentamer composed of alpha and beta subunits.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000274093.

Structurei

3D structure databases

ProteinModelPortaliO75311.
SMRiO75311. Positions 47-345, 421-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG265706.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiO75311.
KOiK05195.
OMAiPKNSDEM.
OrthoDBiEOG712TVZ.
PhylomeDBiO75311.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008130. Glycine_rcpt_A3.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01676. GLYRALPHA3.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha-3L (identifier: O75311-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAHVRHFRTL VSGFYFWEAA LLLSLVATKE TDSARSRSAP MSPSDFLDKL
60 70 80 90 100
MGRTSGYDAR IRPNFKGPPV NVTCNIFINS FGSIAETTMD YRVNIFLRQK
110 120 130 140 150
WNDPRLAYSE YPDDSLDLDP SMLDSIWKPD LFFANEKGAN FHEVTTDNKL
160 170 180 190 200
LRIFKNGNVL YSIRLTLTLS CPMDLKNFPM DVQTCIMQLE SFGYTMNDLI
210 220 230 240 250
FEWQDEAPVQ VAEGLTLPQF LLKEEKDLRY CTKHYNTGKF TCIEVRFHLE
260 270 280 290 300
RQMGYYLIQM YIPSLLIVIL SWVSFWINMD AAPARVALGI TTVLTMTTQS
310 320 330 340 350
SGSRASLPKV SYVKAIDIWM AVCLLFVFSA LLEYAAVNFV SRQHKELLRF
360 370 380 390 400
RRKRKNKTEA FALEKFYRFS DMDDEVRESR FSFTAYGMGP CLQAKDGMTP
410 420 430 440 450
KGPNHPVQVM PKSPDEMRKV FIDRAKKIDT ISRACFPLAF LIFNIFYWVI
460
YKILRHEDIH QQQD
Length:464
Mass (Da):53,800
Last modified:October 17, 2006 - v2
Checksum:i8E17A30B3C6E648D
GO
Isoform Alpha-3K (identifier: O75311-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     358-372: Missing.

Show »
Length:449
Mass (Da):51,963
Checksum:i342BB384CCA4D33E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti460 – 4601H → HH in AAC39919 (PubMed:9677400).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei358 – 37215Missing in isoform Alpha-3K. 1 PublicationVSP_000084Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017724
, AF017715, AF017716, AF017717, AF017718, AF017719, AF017720, AF017721, AF017722, AF017723 Genomic DNA. Translation: AAC39919.1.
U93917 mRNA. Translation: AAC39917.1.
CH471056 Genomic DNA. Translation: EAX04730.1.
CH471056 Genomic DNA. Translation: EAX04732.1.
BC036086 mRNA. Translation: AAH36086.1.
CCDSiCCDS3822.1. [O75311-1]
CCDS43283.1. [O75311-2]
RefSeqiNP_001036008.1. NM_001042543.2. [O75311-2]
NP_006520.2. NM_006529.3. [O75311-1]
UniGeneiHs.413099.

Genome annotation databases

EnsembliENST00000274093; ENSP00000274093; ENSG00000145451. [O75311-1]
ENST00000340217; ENSP00000345284; ENSG00000145451. [O75311-2]
GeneIDi8001.
KEGGihsa:8001.
UCSCiuc003ity.1. human. [O75311-1]
uc003itz.1. human. [O75311-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017724
, AF017715, AF017716, AF017717, AF017718, AF017719, AF017720, AF017721, AF017722, AF017723 Genomic DNA. Translation: AAC39919.1.
U93917 mRNA. Translation: AAC39917.1.
CH471056 Genomic DNA. Translation: EAX04730.1.
CH471056 Genomic DNA. Translation: EAX04732.1.
BC036086 mRNA. Translation: AAH36086.1.
CCDSiCCDS3822.1. [O75311-1]
CCDS43283.1. [O75311-2]
RefSeqiNP_001036008.1. NM_001042543.2. [O75311-2]
NP_006520.2. NM_006529.3. [O75311-1]
UniGeneiHs.413099.

3D structure databases

ProteinModelPortaliO75311.
SMRiO75311. Positions 47-345, 421-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000274093.

Chemistry

BindingDBiO75311.
ChEMBLiCHEMBL1075092.
DrugBankiDB00145. Glycine.
DB00602. Ivermectin.
DB00431. Lindane.
GuidetoPHARMACOLOGYi425.

Protein family/group databases

TCDBi1.A.9.3.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

PTM databases

PhosphoSiteiO75311.

Proteomic databases

PaxDbiO75311.
PRIDEiO75311.

Protocols and materials databases

DNASUi8001.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274093; ENSP00000274093; ENSG00000145451. [O75311-1]
ENST00000340217; ENSP00000345284; ENSG00000145451. [O75311-2]
GeneIDi8001.
KEGGihsa:8001.
UCSCiuc003ity.1. human. [O75311-1]
uc003itz.1. human. [O75311-2]

Organism-specific databases

CTDi8001.
GeneCardsiGC04M175558.
HGNCiHGNC:4328. GLRA3.
HPAiHPA014805.
MIMi600421. gene.
neXtProtiNX_O75311.
PharmGKBiPA28729.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG265706.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiO75311.
KOiK05195.
OMAiPKNSDEM.
OrthoDBiEOG712TVZ.
PhylomeDBiO75311.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiREACT_25387. Ligand-gated ion channel transport.

Miscellaneous databases

GeneWikiiGLRA3.
GenomeRNAii8001.
NextBioi30552.
PROiO75311.
SOURCEiSearch...

Gene expression databases

BgeeiO75311.
CleanExiHS_GLRA3.
ExpressionAtlasiO75311. baseline and differential.
GenevestigatoriO75311.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008130. Glycine_rcpt_A3.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01676. GLYRALPHA3.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human glycine receptor subunit alpha3. GLRA3 gene structure, chromosomal localization, and functional characterization of alternative transcripts."
    Nikolic Z., Laube B., Weber R.G., Lichter P., Kioschis P., Poustka A., Muelhardt C., Becker C.-M.
    J. Biol. Chem. 273:19708-19714(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS ALPHA-3K AND ALPHA-3L).
    Tissue: Fetal brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-3L).
    Tissue: Brain.

Entry informationi

Entry nameiGLRA3_HUMAN
AccessioniPrimary (citable) accession number: O75311
Secondary accession number(s): D3DP44, O75816, Q5D0E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 17, 2006
Last modified: March 4, 2015
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.