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O75311

- GLRA3_HUMAN

UniProt

O75311 - GLRA3_HUMAN

Protein

Glycine receptor subunit alpha-3

Gene

GLRA3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

    GO - Molecular functioni

    1. extracellular-glycine-gated chloride channel activity Source: InterPro
    2. glycine binding Source: InterPro
    3. transmitter-gated ion channel activity Source: InterPro

    GO - Biological processi

    1. ion transmembrane transport Source: Reactome
    2. transmembrane transport Source: Reactome

    Keywords - Molecular functioni

    Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Enzyme and pathway databases

    ReactomeiREACT_25387. Ligand-gated ion channel transport.

    Protein family/group databases

    TCDBi1.A.9.3.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine receptor subunit alpha-3
    Gene namesi
    Name:GLRA3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:4328. GLRA3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. chloride channel complex Source: UniProtKB-KW
    3. integral component of plasma membrane Source: InterPro
    4. plasma membrane Source: Reactome
    5. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28729.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 464431Glycine receptor subunit alpha-3PRO_0000000419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi171 ↔ 185By similarity
    Disulfide bondi231 ↔ 242By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO75311.
    PRIDEiO75311.

    PTM databases

    PhosphoSiteiO75311.

    Expressioni

    Tissue specificityi

    Widely distributed throughout the central nervous system.

    Gene expression databases

    ArrayExpressiO75311.
    BgeeiO75311.
    CleanExiHS_GLRA3.
    GenevestigatoriO75311.

    Organism-specific databases

    HPAiHPA014805.

    Interactioni

    Subunit structurei

    Pentamer composed of alpha and beta subunits.By similarity

    Protein-protein interaction databases

    STRINGi9606.ENSP00000274093.

    Structurei

    3D structure databases

    ProteinModelPortaliO75311.
    SMRiO75311. Positions 54-345, 421-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini34 – 252219ExtracellularCuratedAdd
    BLAST
    Topological domaini342 – 43392CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei253 – 27927HelicalCuratedAdd
    BLAST
    Transmembranei286 – 30318HelicalCuratedAdd
    BLAST
    Transmembranei315 – 34127HelicalCuratedAdd
    BLAST
    Transmembranei434 – 45118HelicalCuratedAdd
    BLAST

    Family & Domainsi

    Domaini

    The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG265706.
    HOGENOMiHOG000231336.
    HOVERGENiHBG051707.
    InParanoidiO75311.
    KOiK05195.
    OMAiPKNSDEM.
    OrthoDBiEOG712TVZ.
    PhylomeDBiO75311.
    TreeFamiTF315453.

    Family and domain databases

    Gene3Di2.70.170.10. 1 hit.
    InterProiIPR006028. GABAA/Glycine_rcpt.
    IPR008127. Glycine_rcpt_A.
    IPR008130. Glycine_rcpt_A3.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00253. GABAARECEPTR.
    PR01673. GLYRALPHA.
    PR01676. GLYRALPHA3.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha-3L (identifier: O75311-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAHVRHFRTL VSGFYFWEAA LLLSLVATKE TDSARSRSAP MSPSDFLDKL    50
    MGRTSGYDAR IRPNFKGPPV NVTCNIFINS FGSIAETTMD YRVNIFLRQK 100
    WNDPRLAYSE YPDDSLDLDP SMLDSIWKPD LFFANEKGAN FHEVTTDNKL 150
    LRIFKNGNVL YSIRLTLTLS CPMDLKNFPM DVQTCIMQLE SFGYTMNDLI 200
    FEWQDEAPVQ VAEGLTLPQF LLKEEKDLRY CTKHYNTGKF TCIEVRFHLE 250
    RQMGYYLIQM YIPSLLIVIL SWVSFWINMD AAPARVALGI TTVLTMTTQS 300
    SGSRASLPKV SYVKAIDIWM AVCLLFVFSA LLEYAAVNFV SRQHKELLRF 350
    RRKRKNKTEA FALEKFYRFS DMDDEVRESR FSFTAYGMGP CLQAKDGMTP 400
    KGPNHPVQVM PKSPDEMRKV FIDRAKKIDT ISRACFPLAF LIFNIFYWVI 450
    YKILRHEDIH QQQD 464
    Length:464
    Mass (Da):53,800
    Last modified:October 17, 2006 - v2
    Checksum:i8E17A30B3C6E648D
    GO
    Isoform Alpha-3K (identifier: O75311-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         358-372: Missing.

    Show »
    Length:449
    Mass (Da):51,963
    Checksum:i342BB384CCA4D33E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti460 – 4601H → HH in AAC39919. (PubMed:9677400)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei358 – 37215Missing in isoform Alpha-3K. 1 PublicationVSP_000084Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017724
    , AF017715, AF017716, AF017717, AF017718, AF017719, AF017720, AF017721, AF017722, AF017723 Genomic DNA. Translation: AAC39919.1.
    U93917 mRNA. Translation: AAC39917.1.
    CH471056 Genomic DNA. Translation: EAX04730.1.
    CH471056 Genomic DNA. Translation: EAX04732.1.
    BC036086 mRNA. Translation: AAH36086.1.
    CCDSiCCDS3822.1. [O75311-1]
    CCDS43283.1. [O75311-2]
    RefSeqiNP_001036008.1. NM_001042543.2. [O75311-2]
    NP_006520.2. NM_006529.3. [O75311-1]
    UniGeneiHs.413099.

    Genome annotation databases

    EnsembliENST00000274093; ENSP00000274093; ENSG00000145451. [O75311-1]
    ENST00000340217; ENSP00000345284; ENSG00000145451. [O75311-2]
    GeneIDi8001.
    KEGGihsa:8001.
    UCSCiuc003ity.1. human. [O75311-1]
    uc003itz.1. human. [O75311-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017724
    , AF017715 , AF017716 , AF017717 , AF017718 , AF017719 , AF017720 , AF017721 , AF017722 , AF017723 Genomic DNA. Translation: AAC39919.1 .
    U93917 mRNA. Translation: AAC39917.1 .
    CH471056 Genomic DNA. Translation: EAX04730.1 .
    CH471056 Genomic DNA. Translation: EAX04732.1 .
    BC036086 mRNA. Translation: AAH36086.1 .
    CCDSi CCDS3822.1. [O75311-1 ]
    CCDS43283.1. [O75311-2 ]
    RefSeqi NP_001036008.1. NM_001042543.2. [O75311-2 ]
    NP_006520.2. NM_006529.3. [O75311-1 ]
    UniGenei Hs.413099.

    3D structure databases

    ProteinModelPortali O75311.
    SMRi O75311. Positions 54-345, 421-458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000274093.

    Chemistry

    BindingDBi O75311.
    ChEMBLi CHEMBL1075092.
    DrugBanki DB00145. Glycine.
    DB00602. Ivermectin.
    DB00431. Lindane.
    GuidetoPHARMACOLOGYi 425.

    Protein family/group databases

    TCDBi 1.A.9.3.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

    PTM databases

    PhosphoSitei O75311.

    Proteomic databases

    PaxDbi O75311.
    PRIDEi O75311.

    Protocols and materials databases

    DNASUi 8001.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000274093 ; ENSP00000274093 ; ENSG00000145451 . [O75311-1 ]
    ENST00000340217 ; ENSP00000345284 ; ENSG00000145451 . [O75311-2 ]
    GeneIDi 8001.
    KEGGi hsa:8001.
    UCSCi uc003ity.1. human. [O75311-1 ]
    uc003itz.1. human. [O75311-2 ]

    Organism-specific databases

    CTDi 8001.
    GeneCardsi GC04M175558.
    HGNCi HGNC:4328. GLRA3.
    HPAi HPA014805.
    MIMi 600421. gene.
    neXtProti NX_O75311.
    PharmGKBi PA28729.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG265706.
    HOGENOMi HOG000231336.
    HOVERGENi HBG051707.
    InParanoidi O75311.
    KOi K05195.
    OMAi PKNSDEM.
    OrthoDBi EOG712TVZ.
    PhylomeDBi O75311.
    TreeFami TF315453.

    Enzyme and pathway databases

    Reactomei REACT_25387. Ligand-gated ion channel transport.

    Miscellaneous databases

    GeneWikii GLRA3.
    GenomeRNAii 8001.
    NextBioi 30552.
    PROi O75311.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75311.
    Bgeei O75311.
    CleanExi HS_GLRA3.
    Genevestigatori O75311.

    Family and domain databases

    Gene3Di 2.70.170.10. 1 hit.
    InterProi IPR006028. GABAA/Glycine_rcpt.
    IPR008127. Glycine_rcpt_A.
    IPR008130. Glycine_rcpt_A3.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00253. GABAARECEPTR.
    PR01673. GLYRALPHA.
    PR01676. GLYRALPHA3.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human glycine receptor subunit alpha3. GLRA3 gene structure, chromosomal localization, and functional characterization of alternative transcripts."
      Nikolic Z., Laube B., Weber R.G., Lichter P., Kioschis P., Poustka A., Muelhardt C., Becker C.-M.
      J. Biol. Chem. 273:19708-19714(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS ALPHA-3K AND ALPHA-3L).
      Tissue: Fetal brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-3L).
      Tissue: Brain.

    Entry informationi

    Entry nameiGLRA3_HUMAN
    AccessioniPrimary (citable) accession number: O75311
    Secondary accession number(s): D3DP44, O75816, Q5D0E3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The alpha subunit binds strychnine.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3