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O75311 (GLRA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine receptor subunit alpha-3
Gene names
Name:GLRA3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

Subunit structure

Pentamer composed of alpha and beta subunits By similarity.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue specificity

Widely distributed throughout the central nervous system.

Domain

The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.

Miscellaneous

The alpha subunit binds strychnine.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA3 sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha-3L (identifier: O75311-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha-3K (identifier: O75311-2)

The sequence of this isoform differs from the canonical sequence as follows:
     358-372: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 464431Glycine receptor subunit alpha-3
PRO_0000000419

Regions

Topological domain34 – 252219Extracellular Probable
Transmembrane253 – 27927Helical; Probable
Transmembrane286 – 30318Helical; Probable
Transmembrane315 – 34127Helical; Probable
Topological domain342 – 43392Cytoplasmic Probable
Transmembrane434 – 45118Helical; Probable

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Disulfide bond171 ↔ 185 By similarity
Disulfide bond231 ↔ 242 By similarity

Natural variations

Alternative sequence358 – 37215Missing in isoform Alpha-3K.
VSP_000084

Experimental info

Sequence conflict4601H → HH in AAC39919. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha-3L [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 8E17A30B3C6E648D

FASTA46453,800
        10         20         30         40         50         60 
MAHVRHFRTL VSGFYFWEAA LLLSLVATKE TDSARSRSAP MSPSDFLDKL MGRTSGYDAR 

        70         80         90        100        110        120 
IRPNFKGPPV NVTCNIFINS FGSIAETTMD YRVNIFLRQK WNDPRLAYSE YPDDSLDLDP 

       130        140        150        160        170        180 
SMLDSIWKPD LFFANEKGAN FHEVTTDNKL LRIFKNGNVL YSIRLTLTLS CPMDLKNFPM 

       190        200        210        220        230        240 
DVQTCIMQLE SFGYTMNDLI FEWQDEAPVQ VAEGLTLPQF LLKEEKDLRY CTKHYNTGKF 

       250        260        270        280        290        300 
TCIEVRFHLE RQMGYYLIQM YIPSLLIVIL SWVSFWINMD AAPARVALGI TTVLTMTTQS 

       310        320        330        340        350        360 
SGSRASLPKV SYVKAIDIWM AVCLLFVFSA LLEYAAVNFV SRQHKELLRF RRKRKNKTEA 

       370        380        390        400        410        420 
FALEKFYRFS DMDDEVRESR FSFTAYGMGP CLQAKDGMTP KGPNHPVQVM PKSPDEMRKV 

       430        440        450        460 
FIDRAKKIDT ISRACFPLAF LIFNIFYWVI YKILRHEDIH QQQD 

« Hide

Isoform Alpha-3K [UniParc].

Checksum: 342BB384CCA4D33E
Show »

FASTA44951,963

References

« Hide 'large scale' references
[1]"The human glycine receptor subunit alpha3. GLRA3 gene structure, chromosomal localization, and functional characterization of alternative transcripts."
Nikolic Z., Laube B., Weber R.G., Lichter P., Kioschis P., Poustka A., Muelhardt C., Becker C.-M.
J. Biol. Chem. 273:19708-19714(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS ALPHA-3K AND ALPHA-3L).
Tissue: Fetal brain.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA-3L).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017724 expand/collapse EMBL AC list , AF017715, AF017716, AF017717, AF017718, AF017719, AF017720, AF017721, AF017722, AF017723 Genomic DNA. Translation: AAC39919.1.
U93917 mRNA. Translation: AAC39917.1.
CH471056 Genomic DNA. Translation: EAX04730.1.
CH471056 Genomic DNA. Translation: EAX04732.1.
BC036086 mRNA. Translation: AAH36086.1.
CCDSCCDS3822.1. [O75311-1]
CCDS43283.1. [O75311-2]
RefSeqNP_001036008.1. NM_001042543.2. [O75311-2]
NP_006520.2. NM_006529.3. [O75311-1]
UniGeneHs.413099.

3D structure databases

ProteinModelPortalO75311.
SMRO75311. Positions 54-345, 421-458.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000274093.

Chemistry

BindingDBO75311.
ChEMBLCHEMBL1075092.
DrugBankDB00145. Glycine.
GuidetoPHARMACOLOGY425.

Protein family/group databases

TCDB1.A.9.3.1. the neurotransmitter receptor, cys loop, ligand-gated ion channel (lic) family.

PTM databases

PhosphoSiteO75311.

Proteomic databases

PaxDbO75311.
PRIDEO75311.

Protocols and materials databases

DNASU8001.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274093; ENSP00000274093; ENSG00000145451. [O75311-1]
ENST00000340217; ENSP00000345284; ENSG00000145451. [O75311-2]
GeneID8001.
KEGGhsa:8001.
UCSCuc003ity.1. human. [O75311-1]
uc003itz.1. human. [O75311-2]

Organism-specific databases

CTD8001.
GeneCardsGC04M175558.
HGNCHGNC:4328. GLRA3.
HPAHPA014805.
MIM600421. gene.
neXtProtNX_O75311.
PharmGKBPA28729.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG265706.
HOGENOMHOG000231336.
HOVERGENHBG051707.
InParanoidO75311.
KOK05195.
OMAPKNSDEM.
OrthoDBEOG712TVZ.
PhylomeDBO75311.
TreeFamTF315453.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressO75311.
BgeeO75311.
CleanExHS_GLRA3.
GenevestigatorO75311.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008130. Glycine_rcpt_A3.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01676. GLYRALPHA3.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGLRA3.
GenomeRNAi8001.
NextBio30552.
PROO75311.
SOURCESearch...

Entry information

Entry nameGLRA3_HUMAN
AccessionPrimary (citable) accession number: O75311
Secondary accession number(s): D3DP44, O75816, Q5D0E3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 17, 2006
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM