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O75191

- XYLB_HUMAN

UniProt

O75191 - XYLB_HUMAN

Protein

Xylulose kinase

Gene

XYLB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 3 (05 May 2009)
      Previous versions | rss
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    Functioni

    Phosphorylates D-xylulose to produce D-xylulose 5-phosphate, a molecule that may play an important role in the regulation of glucose metabolism and lipogenesis.1 Publication

    Catalytic activityi

    ATP + D-xylulose = ADP + D-xylulose 5-phosphate.1 Publication

    Kineticsi

    1. KM=24 µM for D-xylulose1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991Substrate
    Binding sitei170 – 1701Substrate
    Binding sitei280 – 2801Substrate
    Binding sitei281 – 2811Substrate
    Binding sitei355 – 3551ATP
    Binding sitei445 – 4451ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi441 – 4422ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: UniProtKB-KW
    3. phosphotransferase activity, alcohol group as acceptor Source: InterPro
    4. xylulokinase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: ProtInc
    2. carbohydrate phosphorylation Source: GOC
    3. D-xylose metabolic process Source: UniProtKB-KW
    4. generation of precursor metabolites and energy Source: ProtInc
    5. xylulose catabolic process Source: BHF-UCL
    6. xylulose metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Xylose metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xylulose kinase (EC:2.7.1.17)
    Short name:
    Xylulokinase
    Gene namesi
    Name:XYLB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:12839. XYLB.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37430.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 536536Xylulose kinasePRO_0000230985Add
    BLAST

    Proteomic databases

    MaxQBiO75191.
    PaxDbiO75191.
    PRIDEiO75191.

    PTM databases

    PhosphoSiteiO75191.

    Expressioni

    Gene expression databases

    ArrayExpressiO75191.
    BgeeiO75191.
    CleanExiHS_XYLB.
    GenevestigatoriO75191.

    Organism-specific databases

    HPAiHPA037863.
    HPA038415.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi115268. 2 interactions.
    STRINGi9606.ENSP00000207870.

    Structurei

    Secondary structure

    1
    536
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi9 – 157
    Beta strandi17 – 2610
    Beta strandi31 – 388
    Helixi39 – 424
    Helixi44 – 463
    Beta strandi52 – 543
    Beta strandi61 – 644
    Helixi65 – 8117
    Helixi86 – 883
    Beta strandi89 – 968
    Beta strandi101 – 1055
    Helixi108 – 1136
    Helixi121 – 1255
    Beta strandi132 – 1354
    Helixi143 – 15311
    Helixi156 – 1638
    Helixi172 – 18211
    Helixi184 – 1885
    Beta strandi190 – 1956
    Helixi196 – 20510
    Helixi213 – 2164
    Beta strandi218 – 2203
    Turni224 – 2274
    Helixi231 – 2377
    Helixi241 – 2455
    Beta strandi254 – 2574
    Helixi260 – 2667
    Beta strandi273 – 2753
    Helixi280 – 2878
    Beta strandi295 – 31117
    Beta strandi316 – 3238
    Beta strandi326 – 33914
    Helixi340 – 35011
    Helixi355 – 3639
    Helixi369 – 3713
    Beta strandi373 – 3797
    Beta strandi382 – 3843
    Beta strandi388 – 3925
    Beta strandi398 – 4003
    Helixi404 – 42522
    Beta strandi436 – 4405
    Helixi441 – 4444
    Helixi446 – 45611
    Beta strandi460 – 4623
    Helixi468 – 48114
    Helixi484 – 4863
    Helixi489 – 4935
    Beta strandi500 – 5034
    Helixi509 – 53022

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BC2X-ray1.97A/B/C1-536[»]
    4BC3X-ray1.68A/B/C1-536[»]
    4BC4X-ray1.79A/B/C1-536[»]
    4BC5X-ray1.98A/B/C1-536[»]
    ProteinModelPortaliO75191.
    SMRiO75191. Positions 6-531.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FGGY kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1070.
    HOGENOMiHOG000174850.
    HOVERGENiHBG053124.
    InParanoidiO75191.
    KOiK00854.
    OMAiGSYSPID.
    PhylomeDBiO75191.
    TreeFamiTF313643.

    Family and domain databases

    InterProiIPR018485. Carb_kinase_FGGY_C.
    IPR018484. Carb_kinase_FGGY_N.
    [Graphical view]
    PfamiPF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O75191-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEHAPRRCC LGWDFSTQQV KVVAVDAELN VFYEESVHFD RDLPEFGTQG    50
    GVHVHKDGLT VTSPVLMWVQ ALDIILEKMK ASGFDFSQVL ALSGAGQQHG 100
    SIYWKAGAQQ ALTSLSPDLR LHQQLQDCFS ISDCPVWMDS STTAQCRQLE 150
    AAVGGAQALS CLTGSRAYER FTGNQIAKIY QQNPEAYSHT ERISLVSSFA 200
    ASLFLGSYSP IDYSDGSGMN LLQIQDKVWS QACLGACAPH LEEKLSPPVP 250
    SCSVVGAISS YYVQRYGFPP GCKVVAFTGD NPASLAGMRL EEGDIAVSLG 300
    TSDTLFLWLQ EPMPALEGHI FCNPVDSQHY MALLCFKNGS LMREKIRNES 350
    VSRSWSDFSK ALQSTEMGNG GNLGFYFDVM EITPEIIGRH RFNTENHKVA 400
    AFPGDVEVRA LIEGQFMAKR IHAEGLGYRV MSKTKILATG GASHNREILQ 450
    VLADVFDAPV YVIDTANSAC VGSAYRAFHG LAGGTDVPFS EVVKLAPNPR 500
    LAATPSPGAS QVYEALLPQY AKLEQRILSQ TRGPPE 536
    Length:536
    Mass (Da):58,382
    Last modified:May 5, 2009 - v3
    Checksum:iE2FC1C45308D71A8
    GO
    Isoform 2 (identifier: O75191-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-137: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:399
    Mass (Da):43,182
    Checksum:iBF7C28142BABD317
    GO

    Sequence cautioni

    The sequence BAA31527.1 differs from that shown. Reason: Probable cloning artifact.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti85 – 851D → E.3 Publications
    Corresponds to variant rs17118 [ dbSNP | Ensembl ].
    VAR_055151
    Natural varianti133 – 1331D → N.
    Corresponds to variant rs2234610 [ dbSNP | Ensembl ].
    VAR_055152
    Natural varianti139 – 1391D → E.
    Corresponds to variant rs151611 [ dbSNP | Ensembl ].
    VAR_055153
    Natural varianti262 – 2621Y → N.1 Publication
    Corresponds to variant rs196380 [ dbSNP | Ensembl ].
    VAR_055154
    Natural varianti348 – 3481N → D.
    Corresponds to variant rs2234622 [ dbSNP | Ensembl ].
    VAR_055155

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 137137Missing in isoform 2. 1 PublicationVSP_055522Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK293325 mRNA. Translation: BAG56843.1.
    AK314386 mRNA. Translation: BAG37011.1.
    AP006191 Genomic DNA. No translation available.
    AP006193 Genomic DNA. No translation available.
    CH471055 Genomic DNA. Translation: EAW64532.1.
    BC137076 mRNA. Translation: AAI37077.1.
    BC137080 mRNA. Translation: AAI37081.1.
    AB015046 mRNA. Translation: BAA31527.1. Sequence problems.
    CCDSiCCDS2678.1.
    RefSeqiNP_005099.2. NM_005108.3.
    UniGeneiHs.659985.

    Genome annotation databases

    EnsembliENST00000207870; ENSP00000207870; ENSG00000093217. [O75191-1]
    GeneIDi9942.
    KEGGihsa:9942.
    UCSCiuc003cic.2. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK293325 mRNA. Translation: BAG56843.1 .
    AK314386 mRNA. Translation: BAG37011.1 .
    AP006191 Genomic DNA. No translation available.
    AP006193 Genomic DNA. No translation available.
    CH471055 Genomic DNA. Translation: EAW64532.1 .
    BC137076 mRNA. Translation: AAI37077.1 .
    BC137080 mRNA. Translation: AAI37081.1 .
    AB015046 mRNA. Translation: BAA31527.1 . Sequence problems.
    CCDSi CCDS2678.1.
    RefSeqi NP_005099.2. NM_005108.3.
    UniGenei Hs.659985.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BC2 X-ray 1.97 A/B/C 1-536 [» ]
    4BC3 X-ray 1.68 A/B/C 1-536 [» ]
    4BC4 X-ray 1.79 A/B/C 1-536 [» ]
    4BC5 X-ray 1.98 A/B/C 1-536 [» ]
    ProteinModelPortali O75191.
    SMRi O75191. Positions 6-531.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115268. 2 interactions.
    STRINGi 9606.ENSP00000207870.

    PTM databases

    PhosphoSitei O75191.

    Proteomic databases

    MaxQBi O75191.
    PaxDbi O75191.
    PRIDEi O75191.

    Protocols and materials databases

    DNASUi 9942.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000207870 ; ENSP00000207870 ; ENSG00000093217 . [O75191-1 ]
    GeneIDi 9942.
    KEGGi hsa:9942.
    UCSCi uc003cic.2. human.

    Organism-specific databases

    CTDi 9942.
    GeneCardsi GC03P038363.
    H-InvDB HIX0003180.
    HGNCi HGNC:12839. XYLB.
    HPAi HPA037863.
    HPA038415.
    MIMi 604049. gene.
    neXtProti NX_O75191.
    PharmGKBi PA37430.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1070.
    HOGENOMi HOG000174850.
    HOVERGENi HBG053124.
    InParanoidi O75191.
    KOi K00854.
    OMAi GSYSPID.
    PhylomeDBi O75191.
    TreeFami TF313643.

    Miscellaneous databases

    GenomeRNAii 9942.
    NextBioi 37512.
    PROi O75191.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75191.
    Bgeei O75191.
    CleanExi HS_XYLB.
    Genevestigatori O75191.

    Family and domain databases

    InterProi IPR018485. Carb_kinase_FGGY_C.
    IPR018484. Carb_kinase_FGGY_N.
    [Graphical view ]
    Pfami PF02782. FGGY_C. 1 hit.
    PF00370. FGGY_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT GLU-85.
      Tissue: Kidney and Mammary gland.
    2. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLU-85.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung and Testis.
    5. "Genomic structure of a novel human gene (XYLB) on chromosome 3p22-->p21.3 encoding a xylulokinase-like protein."
      Tamari M., Daigo Y., Ishikawa S., Nakamura Y.
      Cytogenet. Cell Genet. 82:101-104(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-512 (ISOFORM 1), VARIANTS GLU-85 AND ASN-262.
      Tissue: Liver.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism."
      Bunker R.D., Bulloch E.M., Dickson J.M., Loomes K.M., Baker E.N.
      J. Biol. Chem. 288:1643-1652(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) IN COMPLEX WITH D-XYLULOSE AND ADP, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiXYLB_HUMAN
    AccessioniPrimary (citable) accession number: O75191
    Secondary accession number(s): B2RAW4, B4DDT2, B9EH64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 92 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3