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O75182 (SIN3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Paired amphipathic helix protein Sin3b
Alternative name(s):
Histone deacetylase complex subunit Sin3b
Transcriptional corepressor Sin3b
Gene names
Name:SIN3B
Synonyms:KIAA0700
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1162 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription By similarity. Ref.2

Subunit structure

Interacts with FOXK1/MNF, MXI, MAD, NCOR1 and SAP30. Interaction with SUDS3 enhances the interaction with HDAC1 to form a complex. Interacts with MAD3, MAD4, MAEL, REST and SETDB1 By similarity. Interacts with RNF220 By similarity. Interacts with HCFC1. Ref.2 Ref.5

Subcellular location

Nucleus By similarity Ref.2.

Post-translational modification

Ubiquitinated by RNF220 that leads to proteasomal degradation By similarity.

Sequence similarities

Contains 3 PAH (paired amphipathic helix) domains.

Sequence caution

The sequence BAA31675.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.5 (identifier: O75182-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 Ref.2 (identifier: O75182-2)

The sequence of this isoform differs from the canonical sequence as follows:
     423-454: Missing.
Note: No experimental confirmation available.
Isoform 3 Ref.4 (identifier: O75182-3)

The sequence of this isoform differs from the canonical sequence as follows:
     354-1162: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11621162Paired amphipathic helix protein Sin3b
PRO_0000121539

Regions

Domain37 – 10771PAH 1
Domain153 – 23886PAH 2
Domain292 – 36978PAH 3
Region59 – 10547Interaction with REST By similarity
Region284 – 540257Interaction with NCOR1 By similarity Ref.2
Region392 – 591200Interaction with SUDS3 and HDAC1 By similarity Ref.2

Natural variations

Alternative sequence354 – 1162809Missing in isoform 3. Ref.4
VSP_014185
Alternative sequence423 – 45432Missing in isoform 2. Ref.2
VSP_014186

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 03D3B24AF1CF40B6

FASTA1,162133,066
        10         20         30         40         50         60 
MAHAGGGSGG SGAGGPAGRG LSGARWGRSG SAGHEKLPVH VEDALTYLDQ VKIRFGSDPA 

        70         80         90        100        110        120 
TYNGFLEIMK EFKSQSIDTP GVIRRVSQLF HEHPDLIVGF NAFLPLGYRI DIPKNGKLNI 

       130        140        150        160        170        180 
QSPLTSQENS HNHGDGAEDF KQQVPYKEDK PQVPLESDSV EFNNAISYVN KIKTRFLDHP 

       190        200        210        220        230        240 
EIYRSFLEIL HTYQKEQLNT RGRPFRGMSE EEVFTEVANL FRGQEDLLSE FGQFLPEAKR 

       250        260        270        280        290        300 
SLFTGNGPCE MHSVQKNEHD KTPEHSRKRS RPSLLRPVSA PAKKKMKLRG TKDLSIAAVG 

       310        320        330        340        350        360 
KYGTLQEFSF FDKVRRVLKS QEVYENFLRC IALFNQELVS GSELLQLVSP FLGKFPELFA 

       370        380        390        400        410        420 
QFKSFLGVKE LSFAPPMSDR SGDGISREID YASCKRIGSS YRALPKTYQQ PKCSGRTAIC 

       430        440        450        460        470        480 
KELDHWTLLQ GSWTDDYCMS KFKNTCWIPG YSAGVLNDTW VSFPSWSEDS TFVSSKKTPY 

       490        500        510        520        530        540 
EEQLHRCEDE RFELDVVLET NLATIRVLES VQKKLSRMAP EDQEKFRLDD SLGGTSEVIQ 

       550        560        570        580        590        600 
RRAIYRIYGD KAPEIIESLK KNPVTAVPVV LKRLKAKEEE WREAQQGFNK IWREQYEKAY 

       610        620        630        640        650        660 
LKSLDHQAVN FKQNDTKALR SKSLLNEIES VYDEHQEQHS EGRSAPSSEP HLIFVYEDRQ 

       670        680        690        700        710        720 
ILEDAAALIS YYVKRQPAIQ KEDQGTIHQL LHQFVPSLFF SQQLDLGASE ESADEDRDSP 

       730        740        750        760        770        780 
QGQTTDPSER KKPAPGPHSS PPEEKGAFGD APATEQPPLP PPAPHKPLDD VYSLFFANNN 

       790        800        810        820        830        840 
WYFFLRLHQT LCSRLLKIYR QAQKQLLEYR TEKEREKLLC EGRREKGSDP AMELRLKQPS 

       850        860        870        880        890        900 
EVELEEYYPA FLDMVRSLLE GSIDPTQYED TLREMFTIHA YVGFTMDKLV QNIARQLHHL 

       910        920        930        940        950        960 
VSDDVCLKVV ELYLNEKKRG AAGGNLSSRC VRAARETSYQ WKAERCMADE NCFKVMFLQR 

       970        980        990       1000       1010       1020 
KGQVIMTIEL LDTEEAQTED PVEVQHLARY VEQYVGTEGA SSSPTEGFLL KPVFLQRNLK 

      1030       1040       1050       1060       1070       1080 
KFRRRWQSEQ ARALRGEARS SWKRLVGVES ACDVDCRFKL STHKMVFIVN SEDYMYRRGT 

      1090       1100       1110       1120       1130       1140 
LCRAKQVQPL VLLRHHQHFE EWHSRWLEDN VTVEAASLVQ DWLMGEEDED MVPCKTLCET 

      1150       1160 
VHVHGLPVTR YRVQYSRRPA SP 

« Hide

Isoform 2 [UniParc].

Checksum: B767339317ECC96D
Show »

FASTA1,130129,359
Isoform 3 [UniParc].

Checksum: 95056B3DCF6C0D53
Show »

FASTA35339,507

References

« Hide 'large scale' references
[1]Ali M., Batra H., Saluja D.
Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 743-1162 (ISOFORMS 1/2).
Tissue: Duodenum, Lung, Lymph and Skin.
[5]"Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1."
Wysocka J., Myers M.P., Laherty C.D., Eisenman R.N., Herr W.
Genes Dev. 17:896-911(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HCFC1.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY706204 mRNA. Translation: AAU01916.1.
AB014600 mRNA. Translation: BAA31675.2. Different initiation.
CH471106 Genomic DNA. Translation: EAW84565.1.
BC005113 mRNA. Translation: AAH05113.1.
BC025026 mRNA. Translation: AAH25026.1.
BC063531 mRNA. Translation: AAH63531.1.
BC110821 mRNA. Translation: AAI10822.1.
RefSeqNP_056075.1. NM_015260.2.
XP_005259889.1. XM_005259832.2.
UniGeneHs.13999.

3D structure databases

ProteinModelPortalO75182.
SMRO75182. Positions 38-114, 156-240, 301-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116901. 44 interactions.
IntActO75182. 7 interactions.
STRING9606.ENSP00000369131.

PTM databases

PhosphoSiteO75182.

Proteomic databases

PaxDbO75182.
PRIDEO75182.

Protocols and materials databases

DNASU23309.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000248054; ENSP00000248054; ENSG00000127511. [O75182-2]
ENST00000379803; ENSP00000369131; ENSG00000127511. [O75182-1]
ENST00000596802; ENSP00000473039; ENSG00000127511. [O75182-3]
GeneID23309.
KEGGhsa:23309.
UCSCuc002new.3. human. [O75182-3]
uc002ney.2. human. [O75182-1]
uc002nez.2. human. [O75182-2]

Organism-specific databases

CTD23309.
GeneCardsGC19P016940.
HGNCHGNC:19354. SIN3B.
HPAHPA050329.
MIM607777. gene.
neXtProtNX_O75182.
PharmGKBPA134909030.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5602.
HOGENOMHOG000230688.
HOVERGENHBG060425.
InParanoidO75182.
OMAGEARSSW.
PhylomeDBO75182.
TreeFamTF106187.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressO75182.
BgeeO75182.
CleanExHS_SIN3B.
GenevestigatorO75182.

Family and domain databases

Gene3D1.20.1160.11. 3 hits.
InterProIPR013194. HDAC_interact.
IPR003822. PAH.
[Graphical view]
PfamPF02671. PAH. 3 hits.
PF08295. Sin3_corepress. 2 hits.
[Graphical view]
SMARTSM00761. HDAC_interact. 1 hit.
[Graphical view]
SUPFAMSSF47762. SSF47762. 3 hits.
PROSITEPS51477. PAH. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSIN3B.
GenomeRNAi23309.
NextBio45174.
PROO75182.
SOURCESearch...

Entry information

Entry nameSIN3B_HUMAN
AccessionPrimary (citable) accession number: O75182
Secondary accession number(s): Q2NL91 expand/collapse secondary AC list , Q68GC2, Q6P4B8, Q8TB34, Q9BSC8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM