CCR4-NOT transcription complex subunit 3

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Reviewed, UniProtKB/Swiss-Prot O75175 (CNOT3_HUMAN)

Last modified December 15, 2009. Version 75. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    CCR4-NOT transcription complex subunit 3
Alternative name(s):
    CCR4-associated factor 3
    Leukocyte receptor cluster member 2

Gene names

Name:	CNOT3
Synonyms:	KIAA0691, LENG2, NOT3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	753 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The CCR4-NOT complex functions as general transcription regulation complex.
Subunit structure	Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Binds TIP120B.
Subcellular location	Cytoplasm Probable. Nucleus Probable.
Tissue specificity	Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Ref.1
Sequence similarities	Belongs to the CNOT2/3/5 family.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB transcription regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
CNOT6	Q9ULM6	1	EBI-743073,EBI-2104530
CNOT6L	Q96LI5	2	EBI-743073,EBI-1046635
TOB1	P50616	2	EBI-743073,EBI-723281

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75175-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75175-2) The sequence of this isoform differs from the canonical sequence as follows: 608-609: TK → PR 610-753: Missing.

Isoform 3 (identifier: O75175-3) The sequence of this isoform differs from the canonical sequence as follows: 1-181: Missing. 680-753: GTKAQYLAAK...YRYLEDRDLQ → PRDVRTAWVD...LSSPLPTPLL
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 753

753

CCR4-NOT transcription complex subunit 3

PRO_0000198333

Regions

Compositional bias

314 – 395

Pro-rich

Compositional bias

397 – 425

Gly/Ser-rich

Compositional bias

457 – 655

199

Pro-rich

Amino acid modifications

Modified residue

292

Phosphothreonine Ref.8

Modified residue

299

Phosphoserine Ref.6 Ref.7

Natural variations

Alternative sequence

1 – 181

181

Missing in isoform 3.

VSP_009919

Alternative sequence

608 – 609

TK → PR in isoform 2.

VSP_009920

Alternative sequence

610 – 753

144

Missing in isoform 2.

VSP_009921

Alternative sequence

680 – 753

GTKAQ…DRDLQ → PRDVRTAWVDSEAGPLRHTS APLPVAHWLTRGPSPALLQQ PQSRPTPLCSSAHSQASLET TGELSSPLPTPLL in isoform 3.

VSP_009922

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified November 1, 1998. Version 1. Checksum: C9D4F8B9FEF6CD90 Show »	FASTA	753	81,872
10 20 30 40 50 60 MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD 70 80 90 100 110 120 QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE 130 140 150 160 170 180 KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV 190 200 210 220 230 240 RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA 250 260 270 280 290 300 TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP 310 320 330 340 350 360 AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP SALGPKASPA 370 380 390 400 410 420 PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG GGSGGGGSSS SSNSSAGGGA 430 440 450 460 470 480 GKQNGATSYS SVVADSPAEV ALSSSGGNNA SSQALGPPSG PHNPPPSTSK EPSAAAPTGA 490 500 510 520 530 540 GGVAPGSGNN SGGPSLLVPL PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL 550 560 570 580 590 600 SSLKSMAERA AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC 610 620 630 640 650 660 PLGPVPLTKE QLYQQAMEEA AWHHMPHPSD SERIRQYLPR NPCPTPPYHH QMPPPHSDTV 670 680 690 700 710 720 EFYQRLSTET LFFIFYYLEG TKAQYLAAKA LKKQSWRFHT KYMMWFQRHE EPKTITDEFE 730 740 750 QGTYIYFDYE KWGQRKKEGF TFEYRYLEDR DLQ « Hide
Isoform 2. Checksum: 507FC8AF634B613C Show »	FASTA	609	64,053
10 20 30 40 50 60 MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD 70 80 90 100 110 120 QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE 130 140 150 160 170 180 KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV 190 200 210 220 230 240 RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA 250 260 270 280 290 300 TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP 310 320 330 340 350 360 AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP SALGPKASPA 370 380 390 400 410 420 PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG GGSGGGGSSS SSNSSAGGGA 430 440 450 460 470 480 GKQNGATSYS SVVADSPAEV ALSSSGGNNA SSQALGPPSG PHNPPPSTSK EPSAAAPTGA 490 500 510 520 530 540 GGVAPGSGNN SGGPSLLVPL PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL 550 560 570 580 590 600 SSLKSMAERA AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC PLGPVPLPR « Hide
Isoform 3. Checksum: B21FEF915F2D01F4 Show »	FASTA	571	58,841
10 20 30 40 50 60 MLETILRMLD NDSILVDAIR KIKDDVEYYV DSSQDPDFEE NEFLYDDLDL EDIPQALVAT 70 80 90 100 110 120 SPPSHSHMED EIFNQSSSTP TSTTSSSPIP PSPANCTTEN SEDDKKRGRS TDSEVSQSPA 130 140 150 160 170 180 KNGSKPVHSN QHPQSPAVPP TYPSGPPPAA SALSTTPGNN GVPAPAAPPS ALGPKASPAP 190 200 210 220 230 240 SHNSGTPAPY AQAVAPPAPS GPSTTQPRPP SVQPSGGGGG GSGGGGSSSS SNSSAGGGAG 250 260 270 280 290 300 KQNGATSYSS VVADSPAEVA LSSSGGNNAS SQALGPPSGP HNPPPSTSKE PSAAAPTGAG 310 320 330 340 350 360 GVAPGSGNNS GGPSLLVPLP VNPPSSPTPS FSDAKAAGAL LNGPPQFSTA PEIKAPEPLS 370 380 390 400 410 420 SLKSMAERAA ISSGIEDPVP TLHLTERDII LSSTSAPPAS AQPPLQLSEV NIPLSLGVCP 430 440 450 460 470 480 LGPVPLTKEQ LYQQAMEEAA WHHMPHPSDS ERIRQYLPRN PCPTPPYHHQ MPPPHSDTVE 490 500 510 520 530 540 FYQRLSTETL FFIFYYLEPR DVRTAWVDSE AGPLRHTSAP LPVAHWLTRG PSPALLQQPQ 550 560 570 SRPTPLCSSA HSQASLETTG ELSSPLPTPL L « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits." Albert T.K., Lemaire M., van Berkum N.L., Gentz R., Collart M.A., Timmers H.T.M. Nucleic Acids Res. 28:809-817(2000) [PubMed: 10637334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CNOT1; CNOT2; CNOT3; CNOT4 AND CNOT8, TISSUE SPECIFICITY.
[2]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"TBP-interacting protein 120B, which is induced in relation to myogenesis, binds to NOT3." Aoki T., Okada N., Wakamatsu T., Tamura T.-A. Biochem. Biophys. Res. Commun. 296:1097-1103(2002) [PubMed: 12207886] [Abstract] Cited for: INTERACTION WITH TIP120B.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-292, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, MASS SPECTROMETRY.

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Cross-references

Sequence databases
	AF180474 mRNA. Translation: AAF29828.1. AB014591 mRNA. Translation: BAA31666.2. Different initiation. AL133647 mRNA. Translation: CAB63766.1. BC016474 mRNA. Translation: AAH16474.1.
IPI	IPI00005015. IPI00186188. IPI00478453.
PIR	T43456.
RefSeq	NP_055331.1.
UniGene	Hs.343571
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O75175. 5 interactions.
STRING	O75175.
PTM databases
PhosphoSite	O75175.
Proteomic databases
PRIDE	O75175.
Genome annotation databases
Ensembl	ENST00000221232; ENSP00000221232; ENSG00000088038; Homo sapiens. [Genome view] ENST00000406403; ENSP00000383954; ENSG00000088038; Homo sapiens. [Genome view]
GeneID	4849.
KEGG	hsa:4849.
UCSC	uc002qdj.1. human.
Organism-specific databases
CTD	4849.
GeneCards	GC19P059333.
H-InvDB	HIX0015432.
HGNC	HGNC:7879. CNOT3.
HPA	HPA006408.
MIM	604910. gene.
PharmGKB	PA26674.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG395803.
HOVERGEN	O75175.
InParanoid	O75175.
OMA	THSHMED.
OrthoDB	EOG9VHNRM.
Gene expression databases
ArrayExpress	O75175.
Bgee	O75175.
CleanEx	HS_CNOT3.
Genevestigator	O75175.
GermOnline	ENSG00000088038. Homo sapiens.
Family and domain databases
InterPro	IPR012270. CCR4-NOT_su3/5. IPR007282. NOT. IPR007207. Not_N. [Graphical view]
Pfam	PF04153. NOT2_3_5. 1 hit. PF04065. Not3. 1 hit. [Graphical view]
PIRSF	PIRSF005290. NOT_su_3_5. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	18674.
SOURCE	Search...

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Entry information

Entry name

CNOT3_HUMAN

Accession

Primary (citable) accession number: O75175
Secondary accession number(s): Q9NZN7, Q9UF76

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	November 1, 1998
Last modified:	December 15, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents