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Protein

Cullin-associated NEDD8-dissociated protein 2

Gene

CAND2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Ubl conjugation pathway

Enzyme and pathway databases

SIGNORiO75155.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-associated NEDD8-dissociated protein 2
Alternative name(s):
Cullin-associated and neddylation-dissociated protein 2
Epididymis tissue protein Li 169
TBP-interacting protein of 120 kDa B
Short name:
TBP-interacting protein 120B
p120 CAND2
Gene namesi
Name:CAND2
Synonyms:KIAA0667, TIP120B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:30689. CAND2.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: UniProtKB
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672208.

Polymorphism and mutation databases

BioMutaiCAND2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 12361235Cullin-associated NEDD8-dissociated protein 2PRO_0000089297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Post-translational modificationi

Ubiquitinated and targeted for proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiO75155.
MaxQBiO75155.
PaxDbiO75155.
PeptideAtlasiO75155.
PRIDEiO75155.

PTM databases

iPTMnetiO75155.
PhosphoSiteiO75155.

Expressioni

Tissue specificityi

Expressed in epididymis (at protein level).1 Publication

Gene expression databases

BgeeiO75155.
CleanExiHS_CAND2.
ExpressionAtlasiO75155. baseline and differential.
GenevisibleiO75155. HS.

Organism-specific databases

HPAiHPA005777.

Interactioni

Subunit structurei

Binds TBP, CNOT3 and UBE3C.By similarity

Protein-protein interaction databases

BioGridi116701. 33 interactions.
IntActiO75155. 2 interactions.
STRINGi9606.ENSP00000387641.

Structurei

3D structure databases

ProteinModelPortaliO75155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 3938HEAT 1Add
BLAST
Repeati44 – 8138HEAT 2Add
BLAST
Repeati83 – 11937HEAT 3Add
BLAST
Repeati129 – 16739HEAT 4Add
BLAST
Repeati171 – 20838HEAT 5Add
BLAST
Repeati210 – 24637HEAT 6Add
BLAST
Repeati254 – 29138HEAT 7Add
BLAST
Repeati327 – 36842HEAT 8Add
BLAST
Repeati372 – 40938HEAT 9Add
BLAST
Repeati432 – 46938HEAT 10Add
BLAST
Repeati517 – 55438HEAT 11Add
BLAST
Repeati565 – 60440HEAT 12Add
BLAST
Repeati608 – 64538HEAT 13Add
BLAST
Repeati648 – 68538HEAT 14Add
BLAST
Repeati690 – 72738HEAT 15Add
BLAST
Repeati731 – 77040HEAT 16Add
BLAST
Repeati772 – 81342HEAT 17Add
BLAST
Repeati857 – 89438HEAT 18Add
BLAST
Repeati896 – 93136HEAT 19Add
BLAST
Repeati933 – 96634HEAT 20Add
BLAST
Repeati967 – 100337HEAT 21Add
BLAST
Repeati1007 – 104438HEAT 22Add
BLAST
Repeati1048 – 108437HEAT 23Add
BLAST
Repeati1105 – 114137HEAT 24Add
BLAST
Repeati1157 – 119438HEAT 25Add
BLAST
Repeati1204 – 123633HEAT 26Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi343 – 3464Poly-Asp
Compositional biasi490 – 4934Poly-Ser
Compositional biasi614 – 6185Poly-Leu

Sequence similaritiesi

Belongs to the CAND family.Curated
Contains 26 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1824. Eukaryota.
ENOG410XPK4. LUCA.
GeneTreeiENSGT00390000017740.
HOGENOMiHOG000264713.
HOVERGENiHBG053467.
InParanoidiO75155.
OMAiVEDFCNL.
OrthoDBiEOG77HDCZ.
PhylomeDBiO75155.
TreeFamiTF300355.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013932. TATA-bd_TIP120.
[Graphical view]
PfamiPF08623. TIP120. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O75155-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAAFHISS LLEKMTSSDK DFRFMATSDL MSELQKDSIQ LDEDSERKVV
60 70 80 90 100
KMLLRLLEDK NGEVQNLAVK CLGPLVVKVK EYQVETIVDT LCTNMRSDKE
110 120 130 140 150
QLRDIAGIGL KTVLSELPPA ATGSGLATNV CRKITGQLTS AIAQQEDVAV
160 170 180 190 200
QLEALDILSD MLSRLGVPLG AFHASLLHCL LPQLSSPRLA VRKRAVGALG
210 220 230 240 250
HLAAACSTDL FVELADHLLD RLPGPRVPTS PTAIRTLIQC LGSVGRQAGH
260 270 280 290 300
RLGAHLDRLV PLVEDFCNLD DDELRESCLQ AFEAFLRKCP KEMGPHVPNV
310 320 330 340 350
TSLCLQYIKH DPNYNYDSDE DEEQMETEDS EFSEQESEDE YSDDDDMSWK
360 370 380 390 400
VRRAAAKCIA ALISSRPDLL PDFHCTLAPV LIRRFKEREE NVKADVFTAY
410 420 430 440 450
IVLLRQTQPP KGWLEAMEEP TQTGSNLHML RGQVPLVVKA LQRQLKDRSV
460 470 480 490 500
RARQGCFSLL TELAGVLPGS LAEHMPVLVS GIIFSLADRS SSSTIRMDAL
510 520 530 540 550
AFLQGLLGTE PAEAFHPHLP ILLPPVMACV ADSFYKIAAE ALVVLQELVR
560 570 580 590 600
ALWPLHRPRM LDPEPYVGEM SAVTLARLRA TDLDQEVKER AISCMGHLVG
610 620 630 640 650
HLGDRLGDDL EPTLLLLLDR LRNEITRLPA IKALTLVAVS PLQLDLQPIL
660 670 680 690 700
AEALHILASF LRKNQRALRL ATLAALDALA QSQGLSLPPS AVQAVLAELP
710 720 730 740 750
ALVNESDMHV AQLAVDFLAT VTQAQPASLV EVSGPVLSEL LRLLRSPLLP
760 770 780 790 800
AGVLAAAEGF LQALVGTRPP CVDYAKLISL LTAPVYEQAV DGGPGLHKQV
810 820 830 840 850
FHSLARCVAA LSAACPQEAA STASRLVCDA RSPHSSTGVK VLAFLSLAEV
860 870 880 890 900
GQVAGPGHQR ELKAVLLEAL GSPSEDVRAA ASYALGRVGA GSLPDFLPFL
910 920 930 940 950
LEQIEAEPRR QYLLLHSLRE ALGAAQPDSL KPYAEDIWAL LFQRCEGAEE
960 970 980 990 1000
GTRGVVAECI GKLVLVNPSF LLPRLRKQLA AGRPHTRSTV ITAVKFLISD
1010 1020 1030 1040 1050
QPHPIDPLLK SFIGEFMESL QDPDLNVRRA TLAFFNSAVH NKPSLVRDLL
1060 1070 1080 1090 1100
DDILPLLYQE TKIRRDLIRE VEMGPFKHTV DDGLDVRKAA FECMYSLLES
1110 1120 1130 1140 1150
CLGQLDICEF LNHVEDGLKD HYDIRMLTFI MVARLATLCP APVLQRVDRL
1160 1170 1180 1190 1200
IEPLRATCTA KVKAGSVKQE FEKQDELKRS AMRAVAALLT IPEVGKSPIM
1210 1220 1230
ADFSSQIRSN PELAALFESI QKDSASAPST DSMELS
Length:1,236
Mass (Da):135,256
Last modified:May 5, 2009 - v3
Checksum:i38F43A7C1B8322A8
GO
Isoform 2 (identifier: O75155-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-71: C → W
     72-164: Missing.
     1014-1037: Missing.

Show »
Length:1,119
Mass (Da):122,664
Checksum:i8206517AA2F8DBD4
GO

Sequence cautioni

The sequence AAI36593.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2041A → T in BAA31642 (PubMed:9734811).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti408 – 4081Q → R.1 Publication
Corresponds to variant rs2305398 [ dbSNP | Ensembl ].
VAR_055023
Natural varianti476 – 4761P → L.
Corresponds to variant rs2305397 [ dbSNP | Ensembl ].
VAR_055024
Natural varianti533 – 5331S → P.
Corresponds to variant rs3732675 [ dbSNP | Ensembl ].
VAR_055025
Natural varianti655 – 6551H → R.
Corresponds to variant rs9838943 [ dbSNP | Ensembl ].
VAR_055026
Natural varianti845 – 8451L → F.
Corresponds to variant rs17037287 [ dbSNP | Ensembl ].
VAR_055027
Natural varianti858 – 8581H → P.2 Publications
Corresponds to variant rs3732678 [ dbSNP | Ensembl ].
VAR_055028
Natural varianti990 – 9901V → I.
Corresponds to variant rs3817121 [ dbSNP | Ensembl ].
VAR_055029
Natural varianti1225 – 12251A → T.1 Publication
Corresponds to variant rs12629133 [ dbSNP | Ensembl ].
VAR_055030

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei71 – 711C → W in isoform 2. 1 PublicationVSP_037240
Alternative sequencei72 – 16493Missing in isoform 2. 1 PublicationVSP_037241Add
BLAST
Alternative sequencei1014 – 103724Missing in isoform 2. 1 PublicationVSP_037242Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU727621 mRNA. Translation: ADU87623.1.
AC034198 Genomic DNA. No translation available.
BC136592 mRNA. Translation: AAI36593.1. Different initiation.
AB014567 mRNA. Translation: BAA31642.1.
CCDSiCCDS43053.1. [O75155-2]
CCDS54554.1. [O75155-1]
PIRiT01239.
RefSeqiNP_001155971.1. NM_001162499.1. [O75155-1]
NP_036430.1. NM_012298.2. [O75155-2]
UniGeneiHs.343664.

Genome annotation databases

EnsembliENST00000295989; ENSP00000295989; ENSG00000144712. [O75155-2]
ENST00000456430; ENSP00000387641; ENSG00000144712. [O75155-1]
GeneIDi23066.
KEGGihsa:23066.
UCSCiuc003bxj.3. human. [O75155-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU727621 mRNA. Translation: ADU87623.1.
AC034198 Genomic DNA. No translation available.
BC136592 mRNA. Translation: AAI36593.1. Different initiation.
AB014567 mRNA. Translation: BAA31642.1.
CCDSiCCDS43053.1. [O75155-2]
CCDS54554.1. [O75155-1]
PIRiT01239.
RefSeqiNP_001155971.1. NM_001162499.1. [O75155-1]
NP_036430.1. NM_012298.2. [O75155-2]
UniGeneiHs.343664.

3D structure databases

ProteinModelPortaliO75155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116701. 33 interactions.
IntActiO75155. 2 interactions.
STRINGi9606.ENSP00000387641.

PTM databases

iPTMnetiO75155.
PhosphoSiteiO75155.

Polymorphism and mutation databases

BioMutaiCAND2.

Proteomic databases

EPDiO75155.
MaxQBiO75155.
PaxDbiO75155.
PeptideAtlasiO75155.
PRIDEiO75155.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000295989; ENSP00000295989; ENSG00000144712. [O75155-2]
ENST00000456430; ENSP00000387641; ENSG00000144712. [O75155-1]
GeneIDi23066.
KEGGihsa:23066.
UCSCiuc003bxj.3. human. [O75155-1]

Organism-specific databases

CTDi23066.
GeneCardsiCAND2.
HGNCiHGNC:30689. CAND2.
HPAiHPA005777.
MIMi610403. gene.
neXtProtiNX_O75155.
PharmGKBiPA142672208.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1824. Eukaryota.
ENOG410XPK4. LUCA.
GeneTreeiENSGT00390000017740.
HOGENOMiHOG000264713.
HOVERGENiHBG053467.
InParanoidiO75155.
OMAiVEDFCNL.
OrthoDBiEOG77HDCZ.
PhylomeDBiO75155.
TreeFamiTF300355.

Enzyme and pathway databases

SIGNORiO75155.

Miscellaneous databases

ChiTaRSiCAND2. human.
GenomeRNAii23066.
PROiO75155.
SOURCEiSearch...

Gene expression databases

BgeeiO75155.
CleanExiHS_CAND2.
ExpressionAtlasiO75155. baseline and differential.
GenevisibleiO75155. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013932. TATA-bd_TIP120.
[Graphical view]
PfamiPF08623. TIP120. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
    Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
    Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Epididymis.
  2. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Bienvenut W.V., Ramsay A., Leung H.Y.
    Submitted (MAR-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-14 AND 864-878, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic kidney.
  4. Bienvenut W.V., Waridel P., Quadroni M.
    Submitted (MAR-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-14 AND 606-620, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic kidney.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-1236 (ISOFORM 1), VARIANTS PRO-858 AND THR-1225.
    Tissue: Testis.
  6. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-1236 (ISOFORM 2), VARIANTS ARG-408 AND PRO-858.
    Tissue: Brain.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAND2_HUMAN
AccessioniPrimary (citable) accession number: O75155
Secondary accession number(s): B9EGM9, E9KL24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 5, 2009
Last modified: July 6, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.