Reviewed, UniProtKB/Swiss-Prot O75145 (LIPA3_HUMAN)

Names and origin

Protein names

Recommended name:
    Liprin-alpha-3
Alternative name(s):
    Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3
      Short name=PTPRF-interacting protein alpha-3

Gene names

Name:	PPFIA3
Synonyms:	KIAA0654

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1194 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. Ref.6
Subunit structure	Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Binds RIMS1, RIMS2, RIMS3 and RIMS4 By similarity. Ref.6
Subcellular location	Cytoplasm By similarity. Cell surface By similarity. Note: Colocalizes with PTPRF at the cell surface By similarity.
Tissue specificity	Expressed only in brain. Ref.6
Domain	The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS.
Sequence similarities	Belongs to the liprin family. Liprin-alpha subfamily. Contains 3 SAM (sterile alpha motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PIK3R1	P27986	1	EBI-1763225,EBI-79464

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O75145-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O75145-2)

The sequence of this isoform differs from the canonical sequence as follows:
     937-945: Missing.

Note: May be due to an exon skipping.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1194	1194	Liprin-alpha-3		PRO_0000191029
Regions
	Domain	838 – 904	67	SAM 1
	Domain	953 – 1017	65	SAM 2
	Domain	1041 – 1110	70	SAM 3
	Coiled coil	26 – 133	108	Potential
	Coiled coil	161 – 501	341	Potential
	Coiled coil	596 – 644	49	Potential
	Coiled coil	1014 – 1040	27	Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.7 Ref.10
	Modified residue	207	1	Phosphoserine Ref.10
	Modified residue	508	1	Phosphoserine By similarity
	Modified residue	645	1	Phosphoserine By similarity
	Modified residue	683	1	Phosphoserine Ref.9
	Modified residue	714	1	Phosphothreonine Ref.7 Ref.10 Ref.9
	Modified residue	718	1	Phosphothreonine Ref.7 Ref.10
	Modified residue	1164	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	937 – 945	9	Missing in isoform 2.		VSP_009392
	Natural variant	563	1	A → S: dbSNP rs2303053. Ref.3 Ref.5		VAR_017757
Experimental info
	Sequence conflict	878	1	E → K in AAC26101. Ref.6
	Sequence conflict	1011	1	I → V in AAC26101. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 16, 2004. Version 3. Checksum: 4DD92531AD8AE12D	FASTA	1,194	133,496
	10 20 30 40 50 60 MMCEVMPTIS EDGRRGSALG PDEAGGELER LMVTMLTERE RLLETLREAQ DGLATAQLRL 70 80 90 100 110 120 RELGHEKDSL QRQLSIALPQ EFAALTKELN LCREQLLERE EEIAELKAER NNTRLLLEHL 130 140 150 160 170 180 ECLVSRHERS LRMTVVKRQA QSPGGVSSEV EVLKALKSLF EHHKALDEKV RERLRMALER 190 200 210 220 230 240 VAVLEEELEL SNQETLNLRE QLSRRRSGLE EPGKDGDGQT LANGLGPGGD SNRRTAELEE 250 260 270 280 290 300 ALERQRAEVC QLRERLAVLC RQMSQLEEEL GTAHRELGKA EEANSKLQRD LKEALAQRED 310 320 330 340 350 360 MEERITTLEK RYLSAQREAT SLHDANDKLE NELASKESLY RQSEEKSRQL AEWLDDAKQK 370 380 390 400 410 420 LQQTLQKAET LPEIEAQLAQ RVAALNKAEE RHGNFEERLR QLEAQLEEKN QELQRARQRE 430 440 450 460 470 480 KMNDDHNKRL SETVDKLLSE SNERLQLHLK ERMGALEEKN SLSEEIANMK KLQDELLLNK 490 500 510 520 530 540 EQLLAEMERM QMEIDQLRGR PPSSYSRSLP GSALELRYSQ APTLPSGAHL DPYVAGSGRA 550 560 570 580 590 600 GKRGRWSGVK EEPSKDWERS APAGSIPPPF PGELDGSDEE EAEGMFGAEL LSPSGQADVQ 610 620 630 640 650 660 TLAIMLQEQL EAINKEIKLI QEEKETTEQR AEELESRVSS SGLDSLGRYR SSCSLPPSLT 670 680 690 700 710 720 TSTLASPSPP SSGHSTPRLA PPSPAREGTD KANHVPKEEA GAPRGEGPAI PGDTPPPTPR 730 740 750 760 770 780 SARLERMTQA LALQAGSLED GGPPRGSEGT PDSLHKAPKK KSIKSSIGRL FGKKEKGRMG 790 800 810 820 830 840 PPGRDSSSLA GTPSDETLAT DPLGLAKLTG PGDKDRRNKR KHELLEEACR QGLPFAAWDG 850 860 870 880 890 900 PTVVSWLELW VGMPAWYVAA CRANVKSGAI MANLSDTEIQ REIGISNPLH RLKLRLAIQE 910 920 930 940 950 960 MVSLTSPSAP ASSRTSTGNV WMTHEEMESL TATTKPETKE ISWEQILAYG DMNHEWVGND 970 980 990 1000 1010 1020 WLPSLGLPQY RSYFMESLVD ARMLDHLNKK ELRGQLKMVD SFHRVSLHYG IMCLKRLNYD 1030 1040 1050 1060 1070 1080 RKDLERRREE SQTQIRDVMV WSNERVMGWV SGLGLKEFAT NLTESGVHGA LLALDETFDY 1090 1100 1110 1120 1130 1140 SDLALLLQIP TQNAQARQLL EKEFSNLISL GTDRRLDEDS AKSFSRSPSW RKMFREKDLR 1150 1160 1170 1180 1190 GVTPDSAEML PPNFRSAAAG ALGSPGLPLR KLQPEGQTSG SSRADGVSVR TYSC
	Isoform 2. Checksum: 2D4AAB5416E93397	FASTA	1,185	132,365
	10 20 30 40 50 60 MMCEVMPTIS EDGRRGSALG PDEAGGELER LMVTMLTERE RLLETLREAQ DGLATAQLRL 70 80 90 100 110 120 RELGHEKDSL QRQLSIALPQ EFAALTKELN LCREQLLERE EEIAELKAER NNTRLLLEHL 130 140 150 160 170 180 ECLVSRHERS LRMTVVKRQA QSPGGVSSEV EVLKALKSLF EHHKALDEKV RERLRMALER 190 200 210 220 230 240 VAVLEEELEL SNQETLNLRE QLSRRRSGLE EPGKDGDGQT LANGLGPGGD SNRRTAELEE 250 260 270 280 290 300 ALERQRAEVC QLRERLAVLC RQMSQLEEEL GTAHRELGKA EEANSKLQRD LKEALAQRED 310 320 330 340 350 360 MEERITTLEK RYLSAQREAT SLHDANDKLE NELASKESLY RQSEEKSRQL AEWLDDAKQK 370 380 390 400 410 420 LQQTLQKAET LPEIEAQLAQ RVAALNKAEE RHGNFEERLR QLEAQLEEKN QELQRARQRE 430 440 450 460 470 480 KMNDDHNKRL SETVDKLLSE SNERLQLHLK ERMGALEEKN SLSEEIANMK KLQDELLLNK 490 500 510 520 530 540 EQLLAEMERM QMEIDQLRGR PPSSYSRSLP GSALELRYSQ APTLPSGAHL DPYVAGSGRA 550 560 570 580 590 600 GKRGRWSGVK EEPSKDWERS APAGSIPPPF PGELDGSDEE EAEGMFGAEL LSPSGQADVQ 610 620 630 640 650 660 TLAIMLQEQL EAINKEIKLI QEEKETTEQR AEELESRVSS SGLDSLGRYR SSCSLPPSLT 670 680 690 700 710 720 TSTLASPSPP SSGHSTPRLA PPSPAREGTD KANHVPKEEA GAPRGEGPAI PGDTPPPTPR 730 740 750 760 770 780 SARLERMTQA LALQAGSLED GGPPRGSEGT PDSLHKAPKK KSIKSSIGRL FGKKEKGRMG 790 800 810 820 830 840 PPGRDSSSLA GTPSDETLAT DPLGLAKLTG PGDKDRRNKR KHELLEEACR QGLPFAAWDG 850 860 870 880 890 900 PTVVSWLELW VGMPAWYVAA CRANVKSGAI MANLSDTEIQ REIGISNPLH RLKLRLAIQE 910 920 930 940 950 960 MVSLTSPSAP ASSRTSTGNV WMTHEEMESL TATTKPILAY GDMNHEWVGN DWLPSLGLPQ 970 980 990 1000 1010 1020 YRSYFMESLV DARMLDHLNK KELRGQLKMV DSFHRVSLHY GIMCLKRLNY DRKDLERRRE 1030 1040 1050 1060 1070 1080 ESQTQIRDVM VWSNERVMGW VSGLGLKEFA TNLTESGVHG ALLALDETFD YSDLALLLQI 1090 1100 1110 1120 1130 1140 PTQNAQARQL LEKEFSNLIS LGTDRRLDED SAKSFSRSPS WRKMFREKDL RGVTPDSAEM 1150 1160 1170 1180 LPPNFRSAAA GALGSPGLPL RKLQPEGQTS GSSRADGVSV RTYSC

References

[1]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-1194 (ISOFORM 2), VARIANT SER-563. Tissue: Brain.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT SER-563.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 909-1194 (ISOFORM 2), VARIANT SER-563. Tissue: Brain and Muscle.
[6]	"Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins." Serra-Pages C., Medley Q.G., Tang M., Hart A., Streuli M. J. Biol. Chem. 273:15611-15620(1998) [PubMed: 9624153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 752-1194 (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION, INTERACTION WITH PTPRD; PTPRF AND PTPRS.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; THR-714 AND THR-718, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-683; THR-714 AND SER-1164, MASS SPECTROMETRY.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-207; THR-714 AND THR-718, MASS SPECTROMETRY. Tissue: T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB014554 mRNA. Translation: BAA31629.2. Different initiation. AK023850 mRNA. Translation: BAB14702.1. AK025972 mRNA. No translation available. AK289757 mRNA. Translation: BAF82446.1. CH471177 Genomic DNA. Translation: EAW52457.1. BC021255 mRNA. Translation: AAH21255.2. BC101518 mRNA. Translation: AAI01519.1. BC101520 mRNA. Translation: AAI01521.1. AF034800 mRNA. Translation: AAC26101.1.
IPI	IPI00398364. IPI00398365.
RefSeq	NP_003651.1.
UniGene	Hs.413748
3D structure databases
SMR	O75145. Positions 821-917, 950-1017.
ModBase	Search...
Protein-protein interaction databases
IntAct	O75145. 2 interactions.
STRING	O75145.
PTM databases
PhosphoSite	O75145.
Proteomic databases
PRIDE	O75145.
Genome annotation databases
Ensembl	ENST00000334186; ENSP00000335614; ENSG00000177380; Homo sapiens. [Genome view]
GeneID	8541.
KEGG	hsa:8541.
UCSC	uc002pmr.1. human. uc002pms.2. human.
Organism-specific databases
CTD	8541.
GeneCards	GC19P054314.
H-InvDB	HIX0015319.
HGNC	HGNC:9247. PPFIA3.
MIM	603144. gene.
PharmGKB	PA33568.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15272.
HOGENOM	HBG356868.
HOVERGEN	O75145.
InParanoid	O75145.
OMA	PGGDSNR.
OrthoDB	EOG9M683K.
PhylomeDB	O75145.
Gene expression databases
ArrayExpress	O75145.
Bgee	O75145.
CleanEx	HS_PPFIA3.
Genevestigator	O75145.
GermOnline	ENSG00000177380. Homo sapiens.
Family and domain databases
InterPro	IPR001660. SAM. IPR011510. SAM_2. IPR010993. SAM_homology. IPR021129. SAM_type1. [Graphical view]
Pfam	PF00536. SAM_1. 2 hits. PF07647. SAM_2. 1 hit. [Graphical view]
SMART	SM00454. SAM. 3 hits. [Graphical view]
PROSITE	PS50105. SAM_DOMAIN. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	31992.
SOURCE	Search...

Entry information

Entry name

LIPA3_HUMAN

Accession

Primary (citable) accession number: O75145
Secondary accession number(s): A8K142 , Q3MJA0, Q9H8B5, Q9UEW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	February 9, 2010
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.