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O75145

- LIPA3_HUMAN

UniProt

O75145 - LIPA3_HUMAN

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Protein

Liprin-alpha-3

Gene
PPFIA3, KIAA0654
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.1 Publication

GO - Molecular functioni

  1. protein binding Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Liprin-alpha-3
Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3
Short name:
PTPRF-interacting protein alpha-3
Gene namesi
Name:PPFIA3
Synonyms:KIAA0654
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:9247. PPFIA3.

Subcellular locationi

Cytoplasm By similarity. Cell surface By similarity
Note: Colocalizes with PTPRF at the cell surface By similarity.

GO - Cellular componenti

  1. cell surface Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33568.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11941194Liprin-alpha-3PRO_0000191029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei207 – 2071Phosphoserine1 Publication
Modified residuei645 – 6451Phosphoserine By similarity
Modified residuei714 – 7141Phosphothreonine1 Publication
Modified residuei718 – 7181Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO75145.
PaxDbiO75145.
PRIDEiO75145.

PTM databases

PhosphoSiteiO75145.

Expressioni

Tissue specificityi

Expressed only in brain.1 Publication

Gene expression databases

BgeeiO75145.
CleanExiHS_PPFIA3.
GenevestigatoriO75145.

Organism-specific databases

HPAiHPA043567.
HPA050340.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Binds RIMS1, RIMS2, RIMS3 and RIMS4 By similarity.1 Publication

Protein-protein interaction databases

BioGridi114111. 8 interactions.
IntActiO75145. 2 interactions.
MINTiMINT-3001008.
STRINGi9606.ENSP00000335614.

Structurei

3D structure databases

ProteinModelPortaliO75145.
SMRiO75145. Positions 812-1115.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini838 – 90467SAM 1Add
BLAST
Domaini953 – 101765SAM 2Add
BLAST
Domaini1041 – 111070SAM 3Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 133108 Reviewed predictionAdd
BLAST
Coiled coili161 – 501341 Reviewed predictionAdd
BLAST
Coiled coili596 – 64449 Reviewed predictionAdd
BLAST
Coiled coili1014 – 104027 Reviewed predictionAdd
BLAST

Domaini

The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG322697.
HOGENOMiHOG000017513.
HOVERGENiHBG052330.
InParanoidiO75145.
OMAiDEPSKDW.
OrthoDBiEOG7T7GS7.
PhylomeDBiO75145.
TreeFamiTF314207.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR029515. Liprin.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR021129. SAM_type1.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 1 hit.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O75145-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMCEVMPTIS EDGRRGSALG PDEAGGELER LMVTMLTERE RLLETLREAQ     50
DGLATAQLRL RELGHEKDSL QRQLSIALPQ EFAALTKELN LCREQLLERE 100
EEIAELKAER NNTRLLLEHL ECLVSRHERS LRMTVVKRQA QSPGGVSSEV 150
EVLKALKSLF EHHKALDEKV RERLRMALER VAVLEEELEL SNQETLNLRE 200
QLSRRRSGLE EPGKDGDGQT LANGLGPGGD SNRRTAELEE ALERQRAEVC 250
QLRERLAVLC RQMSQLEEEL GTAHRELGKA EEANSKLQRD LKEALAQRED 300
MEERITTLEK RYLSAQREAT SLHDANDKLE NELASKESLY RQSEEKSRQL 350
AEWLDDAKQK LQQTLQKAET LPEIEAQLAQ RVAALNKAEE RHGNFEERLR 400
QLEAQLEEKN QELQRARQRE KMNDDHNKRL SETVDKLLSE SNERLQLHLK 450
ERMGALEEKN SLSEEIANMK KLQDELLLNK EQLLAEMERM QMEIDQLRGR 500
PPSSYSRSLP GSALELRYSQ APTLPSGAHL DPYVAGSGRA GKRGRWSGVK 550
EEPSKDWERS APAGSIPPPF PGELDGSDEE EAEGMFGAEL LSPSGQADVQ 600
TLAIMLQEQL EAINKEIKLI QEEKETTEQR AEELESRVSS SGLDSLGRYR 650
SSCSLPPSLT TSTLASPSPP SSGHSTPRLA PPSPAREGTD KANHVPKEEA 700
GAPRGEGPAI PGDTPPPTPR SARLERMTQA LALQAGSLED GGPPRGSEGT 750
PDSLHKAPKK KSIKSSIGRL FGKKEKGRMG PPGRDSSSLA GTPSDETLAT 800
DPLGLAKLTG PGDKDRRNKR KHELLEEACR QGLPFAAWDG PTVVSWLELW 850
VGMPAWYVAA CRANVKSGAI MANLSDTEIQ REIGISNPLH RLKLRLAIQE 900
MVSLTSPSAP ASSRTSTGNV WMTHEEMESL TATTKPETKE ISWEQILAYG 950
DMNHEWVGND WLPSLGLPQY RSYFMESLVD ARMLDHLNKK ELRGQLKMVD 1000
SFHRVSLHYG IMCLKRLNYD RKDLERRREE SQTQIRDVMV WSNERVMGWV 1050
SGLGLKEFAT NLTESGVHGA LLALDETFDY SDLALLLQIP TQNAQARQLL 1100
EKEFSNLISL GTDRRLDEDS AKSFSRSPSW RKMFREKDLR GVTPDSAEML 1150
PPNFRSAAAG ALGSPGLPLR KLQPEGQTSG SSRADGVSVR TYSC 1194
Length:1,194
Mass (Da):133,496
Last modified:February 16, 2004 - v3
Checksum:i4DD92531AD8AE12D
GO
Isoform 2 (identifier: O75145-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     937-945: Missing.

Note: May be due to exon skipping.

Show »
Length:1,185
Mass (Da):132,365
Checksum:i2D4AAB5416E93397
GO

Sequence cautioni

The sequence BAA31629.2 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti563 – 5631A → S.3 Publications
Corresponds to variant rs2303053 [ dbSNP | Ensembl ].
VAR_017757

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei937 – 9459Missing in isoform 2. VSP_009392

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti878 – 8781E → K in AAC26101. 1 Publication
Sequence conflicti1011 – 10111I → V in AAC26101. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB014554 mRNA. Translation: BAA31629.2. Different initiation.
AK023850 mRNA. Translation: BAB14702.1.
AK025972 mRNA. No translation available.
AK289757 mRNA. Translation: BAF82446.1.
CH471177 Genomic DNA. Translation: EAW52457.1.
BC021255 mRNA. Translation: AAH21255.2.
BC101518 mRNA. Translation: AAI01519.1.
BC101520 mRNA. Translation: AAI01521.1.
AF034800 mRNA. Translation: AAC26101.1.
CCDSiCCDS12758.1. [O75145-1]
RefSeqiNP_003651.1. NM_003660.3. [O75145-1]
XP_006723513.1. XM_006723450.1. [O75145-2]
UniGeneiHs.413748.
Hs.604638.

Genome annotation databases

EnsembliENST00000334186; ENSP00000335614; ENSG00000177380. [O75145-1]
ENST00000602351; ENSP00000473622; ENSG00000177380. [O75145-2]
ENST00000602848; ENSP00000473418; ENSG00000177380.
ENST00000602897; ENSP00000473423; ENSG00000177380.
GeneIDi8541.
KEGGihsa:8541.
UCSCiuc002pmr.3. human. [O75145-1]
uc002pms.3. human. [O75145-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB014554 mRNA. Translation: BAA31629.2 . Different initiation.
AK023850 mRNA. Translation: BAB14702.1 .
AK025972 mRNA. No translation available.
AK289757 mRNA. Translation: BAF82446.1 .
CH471177 Genomic DNA. Translation: EAW52457.1 .
BC021255 mRNA. Translation: AAH21255.2 .
BC101518 mRNA. Translation: AAI01519.1 .
BC101520 mRNA. Translation: AAI01521.1 .
AF034800 mRNA. Translation: AAC26101.1 .
CCDSi CCDS12758.1. [O75145-1 ]
RefSeqi NP_003651.1. NM_003660.3. [O75145-1 ]
XP_006723513.1. XM_006723450.1. [O75145-2 ]
UniGenei Hs.413748.
Hs.604638.

3D structure databases

ProteinModelPortali O75145.
SMRi O75145. Positions 812-1115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114111. 8 interactions.
IntActi O75145. 2 interactions.
MINTi MINT-3001008.
STRINGi 9606.ENSP00000335614.

PTM databases

PhosphoSitei O75145.

Proteomic databases

MaxQBi O75145.
PaxDbi O75145.
PRIDEi O75145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000334186 ; ENSP00000335614 ; ENSG00000177380 . [O75145-1 ]
ENST00000602351 ; ENSP00000473622 ; ENSG00000177380 . [O75145-2 ]
ENST00000602848 ; ENSP00000473418 ; ENSG00000177380 .
ENST00000602897 ; ENSP00000473423 ; ENSG00000177380 .
GeneIDi 8541.
KEGGi hsa:8541.
UCSCi uc002pmr.3. human. [O75145-1 ]
uc002pms.3. human. [O75145-2 ]

Organism-specific databases

CTDi 8541.
GeneCardsi GC19P049622.
HGNCi HGNC:9247. PPFIA3.
HPAi HPA043567.
HPA050340.
MIMi 603144. gene.
neXtProti NX_O75145.
PharmGKBi PA33568.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG322697.
HOGENOMi HOG000017513.
HOVERGENi HBG052330.
InParanoidi O75145.
OMAi DEPSKDW.
OrthoDBi EOG7T7GS7.
PhylomeDBi O75145.
TreeFami TF314207.

Miscellaneous databases

ChiTaRSi PPFIA3. human.
GenomeRNAii 8541.
NextBioi 31992.
PROi O75145.
SOURCEi Search...

Gene expression databases

Bgeei O75145.
CleanExi HS_PPFIA3.
Genevestigatori O75145.

Family and domain databases

Gene3Di 1.10.150.50. 2 hits.
InterProi IPR029515. Liprin.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR021129. SAM_type1.
[Graphical view ]
PANTHERi PTHR12587. PTHR12587. 1 hit.
Pfami PF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view ]
SMARTi SM00454. SAM. 3 hits.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 3 hits.
PROSITEi PS50105. SAM_DOMAIN. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
    Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-1194 (ISOFORM 2), VARIANT SER-563.
    Tissue: Brain.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT SER-563.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 909-1194 (ISOFORM 2), VARIANT SER-563.
    Tissue: Brain and Muscle.
  6. "Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins."
    Serra-Pages C., Medley Q.G., Tang M., Hart A., Streuli M.
    J. Biol. Chem. 273:15611-15620(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 752-1194 (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION, INTERACTION WITH PTPRD; PTPRF AND PTPRS.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-207; THR-714 AND THR-718, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLIPA3_HUMAN
AccessioniPrimary (citable) accession number: O75145
Secondary accession number(s): A8K142
, Q3MJA0, Q9H8B5, Q9UEW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: September 3, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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