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O75144

- ICOSL_HUMAN

UniProt

O75144 - ICOSL_HUMAN

Protein

ICOS ligand

Gene

ICOSLG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function By similarity.By similarity

    GO - Molecular functioni

    1. receptor binding Source: UniProtKB

    GO - Biological processi

    1. B cell activation Source: UniProtKB-KW
    2. defense response Source: UniProtKB
    3. hyperosmotic response Source: UniProtKB
    4. positive regulation of activated T cell proliferation Source: UniProtKB
    5. positive regulation of interleukin-4 biosynthetic process Source: Ensembl
    6. signal transduction Source: UniProtKB
    7. T cell activation Source: UniProtKB
    8. T cell costimulation Source: Reactome

    Keywords - Biological processi

    Adaptive immunity, B-cell activation, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_19344. Costimulation by the CD28 family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ICOS ligand
    Alternative name(s):
    B7 homolog 2
    Short name:
    B7-H2
    B7-like protein Gl50
    B7-related protein 1
    Short name:
    B7RP-1
    CD_antigen: CD275
    Gene namesi
    Name:ICOSLG
    Synonyms:B7H2, B7RP1, ICOSL, KIAA0653
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:17087. ICOSLG.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. external side of plasma membrane Source: Ensembl
    2. extracellular vesicular exosome Source: UniProt
    3. integral component of membrane Source: UniProtKB
    4. integral component of plasma membrane Source: Ensembl
    5. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134977297.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 302284ICOS ligandPRO_0000014803Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi37 ↔ 113PROSITE-ProRule annotation
    Glycosylationi70 – 701N-linked (GlcNAc...)1 Publication
    Glycosylationi102 – 1021N-linked (GlcNAc...)1 Publication
    Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi158 ↔ 216PROSITE-ProRule annotation
    Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi186 – 1861N-linked (GlcNAc...)1 Publication
    Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO75144.
    PaxDbiO75144.
    PRIDEiO75144.

    Expressioni

    Tissue specificityi

    Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.1 Publication

    Inductioni

    Constitutive expression is further enhanced by treatment with TNF in peripheral blood B-cells and monocytes, while it is decreased in dendritic cells.

    Gene expression databases

    ArrayExpressiO75144.
    BgeeiO75144.
    CleanExiHS_ICOSLG.
    GenevestigatoriO75144.

    Organism-specific databases

    HPAiCAB026037.

    Interactioni

    Protein-protein interaction databases

    BioGridi116900. 1 interaction.
    STRINGi9606.ENSP00000384432.

    Structurei

    3D structure databases

    ProteinModelPortaliO75144.
    SMRiO75144. Positions 22-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 256238ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini278 – 30225CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei257 – 27721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 129111Ig-like V-typeAdd
    BLAST
    Domaini141 – 22787Ig-like C2-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43300.
    HOGENOMiHOG000036913.
    HOVERGENiHBG106483.
    KOiK06710.
    OrthoDBiEOG79KPFZ.
    PhylomeDBiO75144.
    TreeFamiTF331083.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: O75144-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRLGSPGLLF LLFSSLRADT QEKEVRAMVG SDVELSCACP EGSRFDLNDV    50
    YVYWQTSESK TVVTYHIPQN SSLENVDSRY RNRALMSPAG MLRGDFSLRL 100
    FNVTPQDEQK FHCLVLSQSL GFQEVLSVEV TLHVAANFSV PVVSAPHSPS 150
    QDELTFTCTS INGYPRPNVY WINKTDNSLL DQALQNDTVF LNMRGLYDVV 200
    SVLRIARTPS VNIGCCIENV LLQQNLTVGS QTGNDIGERD KITENPVSTG 250
    EKNAATWSIL AVLCLLVVVA VAIGWVCRDR CLQHSYAGAW AVSPETELTG 300
    HV 302
    Length:302
    Mass (Da):33,349
    Last modified:August 14, 2001 - v2
    Checksum:i647934E21B55E34A
    GO
    Isoform 2 (identifier: O75144-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         300-302: GHV → ESWNLLLLLS

    Show »
    Length:309
    Mass (Da):34,225
    Checksum:iD019EC5307D796A5
    GO
    Isoform 3 (identifier: O75144-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         18-134: Missing.

    Show »
    Length:185
    Mass (Da):20,120
    Checksum:i0DAD5D52E6DE5F5F
    GO

    Sequence cautioni

    The sequence BAA31628.1 differs from that shown. Reason: The sequence differs from that shown in position 300 onward for unknown reason.
    The sequence BAA31628.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti128 – 1281V → I.
    Corresponds to variant rs11558819 [ dbSNP | Ensembl ].
    VAR_049880

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei18 – 134117Missing in isoform 3. 1 PublicationVSP_054718Add
    BLAST
    Alternative sequencei300 – 3023GHV → ESWNLLLLLS in isoform 2. 2 PublicationsVSP_002520

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF199028 mRNA. Translation: AAF34739.1.
    AF289028 mRNA. Translation: AAG01176.1.
    AF216749 mRNA. Translation: AAK16241.1.
    AX100595 mRNA. Translation: CAC36465.1.
    AB014553 mRNA. Translation: BAA31628.1. Sequence problems.
    AK090492 mRNA. Translation: BAG52170.1.
    AP001058 Genomic DNA. No translation available.
    AP001059 Genomic DNA. No translation available.
    CH471079 Genomic DNA. Translation: EAX09446.1.
    BC064637 mRNA. Translation: AAH64637.1.
    CCDSiCCDS42952.1. [O75144-1]
    CCDS63377.1. [O75144-3]
    CCDS63379.1. [O75144-2]
    RefSeqiNP_001269979.1. NM_001283050.1. [O75144-2]
    NP_001269980.1. NM_001283051.1. [O75144-3]
    NP_056074.1. NM_015259.5. [O75144-1]
    XP_006710171.1. XM_006710108.1. [O75144-1]
    XP_006723963.1. XM_006723900.1. [O75144-1]
    UniGeneiHs.14155.

    Genome annotation databases

    EnsembliENST00000344330; ENSP00000339477; ENSG00000160223. [O75144-2]
    ENST00000400377; ENSP00000383228; ENSG00000160223. [O75144-3]
    ENST00000407780; ENSP00000384432; ENSG00000160223. [O75144-1]
    GeneIDi102723996.
    23308.
    KEGGihsa:102723996.
    hsa:23308.
    UCSCiuc002zee.3. human. [O75144-1]
    uc002zef.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF199028 mRNA. Translation: AAF34739.1 .
    AF289028 mRNA. Translation: AAG01176.1 .
    AF216749 mRNA. Translation: AAK16241.1 .
    AX100595 mRNA. Translation: CAC36465.1 .
    AB014553 mRNA. Translation: BAA31628.1 . Sequence problems.
    AK090492 mRNA. Translation: BAG52170.1 .
    AP001058 Genomic DNA. No translation available.
    AP001059 Genomic DNA. No translation available.
    CH471079 Genomic DNA. Translation: EAX09446.1 .
    BC064637 mRNA. Translation: AAH64637.1 .
    CCDSi CCDS42952.1. [O75144-1 ]
    CCDS63377.1. [O75144-3 ]
    CCDS63379.1. [O75144-2 ]
    RefSeqi NP_001269979.1. NM_001283050.1. [O75144-2 ]
    NP_001269980.1. NM_001283051.1. [O75144-3 ]
    NP_056074.1. NM_015259.5. [O75144-1 ]
    XP_006710171.1. XM_006710108.1. [O75144-1 ]
    XP_006723963.1. XM_006723900.1. [O75144-1 ]
    UniGenei Hs.14155.

    3D structure databases

    ProteinModelPortali O75144.
    SMRi O75144. Positions 22-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116900. 1 interaction.
    STRINGi 9606.ENSP00000384432.

    Proteomic databases

    MaxQBi O75144.
    PaxDbi O75144.
    PRIDEi O75144.

    Protocols and materials databases

    DNASUi 23308.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000344330 ; ENSP00000339477 ; ENSG00000160223 . [O75144-2 ]
    ENST00000400377 ; ENSP00000383228 ; ENSG00000160223 . [O75144-3 ]
    ENST00000407780 ; ENSP00000384432 ; ENSG00000160223 . [O75144-1 ]
    GeneIDi 102723996.
    23308.
    KEGGi hsa:102723996.
    hsa:23308.
    UCSCi uc002zee.3. human. [O75144-1 ]
    uc002zef.3. human.

    Organism-specific databases

    CTDi 23308.
    GeneCardsi GC21M045642.
    H-InvDB HIX0016164.
    HGNCi HGNC:17087. ICOSLG.
    HPAi CAB026037.
    MIMi 605717. gene.
    neXtProti NX_O75144.
    PharmGKBi PA134977297.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43300.
    HOGENOMi HOG000036913.
    HOVERGENi HBG106483.
    KOi K06710.
    OrthoDBi EOG79KPFZ.
    PhylomeDBi O75144.
    TreeFami TF331083.

    Enzyme and pathway databases

    Reactomei REACT_19344. Costimulation by the CD28 family.

    Miscellaneous databases

    GeneWikii ICOSLG.
    GenomeRNAii 23308.
    NextBioi 45170.
    PROi O75144.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O75144.
    Bgeei O75144.
    CleanExi HS_ICOSLG.
    Genevestigatori O75144.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Costimulation of T cells by B7-H2, a B7-like molecule that binds ICOS."
      Wang S., Zhu G., Chapoval A.I., Dong H., Tamada K., Ni J., Chen L.
      Blood 96:2808-2813(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Dendritic cell.
    2. "Characterization of a new human B7-related protein: B7RP-1 is the ligand to the co-stimulatory protein ICOS."
      Yoshinaga S.K., Zhang M., Pistillo J., Horan T., Khare S.D., Miner K., Sonnenberg M., Boone T., Brankow D., Dai T., Delaney J., Han H., Hui A., Kohno T., Manoukian R., Whoriskey J.S., Coccia M.A.
      Int. Immunol. 12:1439-1447(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
      Tissue: Peripheral blood lymphocyte.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Leukocyte.
    4. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    5. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
      Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    7. "The DNA sequence of human chromosome 21."
      Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
      , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
      Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon.
    10. Cited for: TISSUE SPECIFICITY.
    11. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
      Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
      J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-70; ASN-102 AND ASN-186.
      Tissue: Plasma.

    Entry informationi

    Entry nameiICOSL_HUMAN
    AccessioniPrimary (citable) accession number: O75144
    Secondary accession number(s): A8MUZ1, Q9HD18, Q9NRQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3