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Protein

ICOS ligand

Gene

ICOSLG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • signaling receptor binding Source: UniProtKB

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • B cell activation Source: UniProtKB-KW
  • defense response Source: UniProtKB
  • hyperosmotic response Source: UniProtKB
  • positive regulation of activated T cell proliferation Source: UniProtKB
  • signal transduction Source: UniProtKB
  • T cell activation Source: UniProtKB
  • T cell costimulation Source: Reactome

Keywordsi

Biological processAdaptive immunity, B-cell activation, Immunity

Enzyme and pathway databases

ReactomeiR-HSA-388841 Costimulation by the CD28 family

Names & Taxonomyi

Protein namesi
Recommended name:
ICOS ligand
Alternative name(s):
B7 homolog 2
Short name:
B7-H2
B7-like protein Gl50
B7-related protein 1
Short name:
B7RP-1
CD_antigen: CD275
Gene namesi
Name:ICOSLG
Synonyms:B7H2, B7RP1, ICOSL, KIAA0653
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000160223.16
HostDB:ENSG00000277117.4
HGNCiHGNC:17087 ICOSLG
MIMi605717 gene
neXtProtiNX_O75144

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 256ExtracellularSequence analysisAdd BLAST238
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 302CytoplasmicSequence analysisAdd BLAST25

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi102723996
23308
OpenTargetsiENSG00000160223
ENSG00000277117
PharmGKBiPA134977297

Polymorphism and mutation databases

BioMutaiICOSLG

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001480319 – 302ICOS ligandAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 113PROSITE-ProRule annotation
Glycosylationi70N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi102N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi137N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi158 ↔ 216PROSITE-ProRule annotation
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi186N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi225N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO75144
PaxDbiO75144
PeptideAtlasiO75144
PRIDEiO75144
TopDownProteomicsiO75144-2 [O75144-2]

PTM databases

GlyConnecti740
iPTMnetiO75144
PhosphoSitePlusiO75144
SwissPalmiO75144
UniCarbKBiO75144

Expressioni

Tissue specificityi

Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.1 Publication

Inductioni

Constitutive expression is further enhanced by treatment with TNF in peripheral blood B-cells and monocytes, while it is decreased in dendritic cells.

Gene expression databases

BgeeiENSG00000160223
CleanExiHS_ICOSLG
ExpressionAtlasiO75144 baseline and differential
GenevisibleiO75144 HS

Organism-specific databases

HPAiCAB026037

Interactioni

Subunit structurei

Interacts with CTLA4 (in vitro).1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • signaling receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116900, 8 interactors
IntActiO75144, 2 interactors
STRINGi9606.ENSP00000384432

Structurei

3D structure databases

ProteinModelPortaliO75144
SMRiO75144
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 129Ig-like V-typeAdd BLAST111
Domaini141 – 227Ig-like C2-typeAdd BLAST87

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYI6 Eukaryota
ENOG410Z67K LUCA
GeneTreeiENSGT00700000104325
HOGENOMiHOG000015302
HOVERGENiHBG106483
InParanoidiO75144
KOiK06710
OMAiTELTESW
OrthoDBiEOG091G0IEZ
PhylomeDBiO75144
TreeFamiTF331083

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR013162 CD80_C2-set
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF08205 C2-set_2, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 1 hit
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O75144-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLGSPGLLF LLFSSLRADT QEKEVRAMVG SDVELSCACP EGSRFDLNDV
60 70 80 90 100
YVYWQTSESK TVVTYHIPQN SSLENVDSRY RNRALMSPAG MLRGDFSLRL
110 120 130 140 150
FNVTPQDEQK FHCLVLSQSL GFQEVLSVEV TLHVAANFSV PVVSAPHSPS
160 170 180 190 200
QDELTFTCTS INGYPRPNVY WINKTDNSLL DQALQNDTVF LNMRGLYDVV
210 220 230 240 250
SVLRIARTPS VNIGCCIENV LLQQNLTVGS QTGNDIGERD KITENPVSTG
260 270 280 290 300
EKNAATWSIL AVLCLLVVVA VAIGWVCRDR CLQHSYAGAW AVSPETELTG

HV
Length:302
Mass (Da):33,349
Last modified:August 14, 2001 - v2
Checksum:i647934E21B55E34A
GO
Isoform 2 (identifier: O75144-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     300-302: GHV → ESWNLLLLLS

Show »
Length:309
Mass (Da):34,225
Checksum:iD019EC5307D796A5
GO
Isoform 3 (identifier: O75144-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-134: Missing.

Show »
Length:185
Mass (Da):20,120
Checksum:i0DAD5D52E6DE5F5F
GO

Sequence cautioni

The sequence BAA31628 differs from that shown. The sequence differs from that shown in position 300 onward for unknown reason.Curated
The sequence BAA31628 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049880128V → I. Corresponds to variant dbSNP:rs11558819Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05471818 – 134Missing in isoform 3. 1 PublicationAdd BLAST117
Alternative sequenceiVSP_002520300 – 302GHV → ESWNLLLLLS in isoform 2. 2 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199028 mRNA Translation: AAF34739.1
AF289028 mRNA Translation: AAG01176.1
AF216749 mRNA Translation: AAK16241.1
AX100595 mRNA Translation: CAC36465.1
AB014553 mRNA Translation: BAA31628.1 Sequence problems.
AK090492 mRNA Translation: BAG52170.1
AP001058 Genomic DNA No translation available.
AP001059 Genomic DNA No translation available.
CH471079 Genomic DNA Translation: EAX09446.1
BC064637 mRNA Translation: AAH64637.1
CCDSiCCDS42952.1 [O75144-1]
CCDS63377.1 [O75144-3]
CCDS63379.1 [O75144-2]
RefSeqiNP_001269979.1, NM_001283050.1 [O75144-2]
NP_001269980.1, NM_001283051.1 [O75144-3]
NP_056074.1, NM_015259.5 [O75144-1]
XP_006723963.1, XM_006723900.2 [O75144-1]
UniGeneiHs.14155

Genome annotation databases

EnsembliENST00000344330; ENSP00000339477; ENSG00000160223 [O75144-2]
ENST00000400377; ENSP00000383228; ENSG00000160223 [O75144-3]
ENST00000407780; ENSP00000384432; ENSG00000160223 [O75144-1]
GeneIDi102723996
23308
KEGGihsa:102723996
hsa:23308
UCSCiuc002zee.5 human [O75144-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiICOSL_HUMAN
AccessioniPrimary (citable) accession number: O75144
Secondary accession number(s): A8MUZ1, Q9HD18, Q9NRQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 14, 2001
Last modified: May 23, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

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