O75122B2RTR1F5H604Q7L8F6Q8N6R6Q9BQT3Q9BQT4Q9H7A3Q9NSZ2CLAP2_HUMANCLIP-associating protein 2Cytoplasmic linker-associated protein 2Protein Orbit homolog 2hOrbit2CLASP2KIAA0627Homo sapiensHumanEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomoPrediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)The DNA sequence, annotation and analysis of human chromosome 3.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3)Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts.NUCLEOTIDE SEQUENCE [MRNA] OF 1-9 (ISOFORM 1)NUCLEOTIDE SEQUENCE [MRNA] OF 1-15 (ISOFORM 2)FUNCTIONALTERNATIVE SPLICINGSUBCELLULAR LOCATIONINTERACTION WITH CLIP2; MICROTUBULES AND RSNThe full-ORF clone resource of the German cDNA consortium.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 939-1294 (ISOFORM 1)Complete sequencing and characterization of 21,243 full-length human cDNAs.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1128-1294 (ISOFORM 1)The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance.SUBCELLULAR LOCATIONCLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex.FUNCTIONINTERACTION WITH MAPRE1; MAPRE3; MICROTUBULES AND RSNSUBCELLULAR LOCATIONSpatial regulation of CLASP affinity for microtubules by Rac1 and GSK3beta in migrating epithelial cells.INTERACTION WITH MICROTUBULESSUBCELLULAR LOCATIONPHOSPHORYLATION BY GSK3BERRATUM OF PUBMED:15955847CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta.FUNCTIONIDENTIFICATION BY MASS SPECTROMETRYINTERACTION WITH ERC1; PHLDB2 AND RSNSUBCELLULAR LOCATIONMammalian CLASPs are required for mitotic spindle organization and kinetochore alignment.FUNCTIONSUBCELLULAR LOCATIONMammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function.FUNCTIONSUBCELLULAR LOCATIONAsymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network.FUNCTIONINTERACTION WITH GCC2SUBCELLULAR LOCATIONATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]A quantitative atlas of mitotic phosphorylation.PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370; SER-525; SER-529; SER-596 AND SER-1029IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]An EB1-binding motif acts as a microtubule tip localization signal.INTERACTION WITH MAPRE1SUBCELLULAR LOCATIONDOMAIN MICROTUBULE TIP LOCALIZATION SIGNALMUTAGENESIS OF 496-ILE-PRO-497 AND 519-ILE-PRO-520Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370; SER-596 AND SER-1029IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells.FUNCTIONSUBCELLULAR LOCATIONQuantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1029IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Initial characterization of the human central proteome.IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-370; SER-455 AND SER-596IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]Toward a comprehensive characterization of a human cancer cell phosphoproteome.PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-327; SER-330; SER-360; SER-370; SER-459; SER-463; SER-478; SER-489; SER-507; SER-525; SER-529; SER-585; SER-587; SER-596; SER-621; THR-787; SER-892; SER-952; SER-955; SER-1013 AND SER-1029IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for faithful chromosome segregation in human cells.INTERACTION WITH MTCL2 AND MTCL1CLASP2 has two distinct TOG domains that contribute differently to microtubule dynamics.X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 60-310FUNCTIONSUBUNITINTERACTION WITH MAPRE1DOMAINMUTAGENESIS OF TRP-106; LYS-191; 496-ILE-PRO-497; SER-499; SER-507; 519-ILE-PRO-520; SER-525; SER-529; SER-533; SER-537; SER-541; TRP-667; LYS-833; ARG-838 AND LYS-839Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.Interacts with microtubules (PubMed:11290329, PubMed:15631994, PubMed:15955847, PubMed:26003921). Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends (PubMed:15631994, PubMed:19632184, PubMed:26003921). Interacts with CLIP2, ERC1, MAPRE3, PHLDB2 and RSN (PubMed:11290329, PubMed:15631994). The interaction with ERC1 may be mediated by PHLDB2 (PubMed:16824950). Interacts with GCC2; recruits CLASP2 to Golgi membranes (PubMed:17543864). Interacts with MACF1 (By similarity). Interacts with mtcl2 and MTCL1 (PubMed:33587225).O75122Q15691false3O75122P31947false4O75122P62258false6O75122O55156true3CytoplasmCytoskeletonCytoplasmCytoskeletonMicrotubule organizing centerCentrosomeChromosomeCentromereKinetochoreCytoplasmCytoskeletonSpindleGolgi apparatusGolgi apparatusTrans-Golgi networkCell membraneCell projectionRuffle membraneLocalizes to microtubule plus ends (PubMed:15631994). Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514). In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane.O75122-11CLASP2 gammaO75122-22CLASP2 betaO75122-33Additional isoforms exist.Brain-specific.The two SXIP sequence motifs mediate interaction with MAPRE1; this is necessary for targeting to growing microtubule plus ends.Two TOG regions display structural characteristics similar to HEAT repeat domains and mediate interaction with microtubules.Phosphorylated by GSK3B. Phosphorylation reduces MAPRE1 binding (PubMed:26003921). Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.Belongs to the CLASP family.Extended N-terminus.Truncated N-terminus.3D-structureAlternative splicingCell cycleCell divisionCell membraneCell projectionCentromereChromosomeCytoplasmCytoskeletonGolgi apparatusKinetochoreMembraneMicrotubuleMitosisPhosphoproteinReference proteomeRepeatMAMGDDMRRLICKRICDYMAMGDMEPRSMEYFCAQVQQKDVGGRLQVGQELLLYLGAPGAISDLEEDLGRLGKTVDALTGWVGSSNYRVSLMGLEILSAFVDRLSTRFKSYVAMVIVALIDRMGDAKDKVRDEAQTLILKLMDQVAPPMYIWEQLASGFKHKNFRSREGVCLCLIETLNIFGAQPLVISKLIPHLCILFGDSNSQVRDAAILAIVEIYRHVGEKVRMDLYKRGIPPARLEMIFAKFDEVQSSGGMILSVCKGGADTSDKLDGTASEDGRVCEAFWRSGRTAKLYSVLLLLGDIRTKWEKAIPAAIPSSSDSDSDIPAAIPSSSDSDSDSDSDWEKERAKAMAMGDDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVRSGRLGAGALNAGSYASLEDTSDKLDGTASEDGRVRAKLSAPLAGMGNAKADSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGVQRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLASRHHSRSTGALYAPEVYGASGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALKKPARRRYESYGMHSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFTRMFADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMNEPLKRDSKKDDGDSMCGGPGMSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSDLVAELLKELSNHNERVEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLREILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAAIKMQTKVIERVSKETLNLLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMKLLNLYIKRAQTGSGGADPTTDVSGQS
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