O75113 (N4BP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD4-binding protein 1
Short name=N4BP1

Gene names

Name:	N4BP1
Synonyms:	KIAA0615

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	896 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates By similarity.
Subunit structure	Interacts with NEDD4. Interacts with ITCH (via WW domain 2) By similarity.
Subcellular location	Nucleus › nucleolus By similarity. Nucleus › PML body By similarity. Note: Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated By similarity.
Tissue specificity	Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. Ref.1
Post-translational modification	Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4 Ref.5 Ref.6 Ref.7
Sequence similarities	Belongs to the N4BP1 family.
Sequence caution	The sequence BAA31590.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	PML body Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 896	896	NEDD4-binding protein 1		PRO_0000096680
Amino acid modifications
	Modified residue	150	1	Phosphoserine Ref.4
	Modified residue	226	1	Phosphoserine Ref.5
	Modified residue	242	1	Phosphothreonine Ref.6
	Modified residue	258	1	Phosphoserine Ref.5 Ref.7
Experimental info
	Sequence conflict	62	1	V → E in BAA31590. Ref.1
	Sequence conflict	62	1	V → E in AAI52473. Ref.3
	Sequence conflict	600	1	L → P in AAI08289. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O75113 [UniParc]. Last modified November 24, 2009. Version 4. Checksum: 171EDCC9D3BCF3D3	FASTA	896	100,379
	10 20 30 40 50 60 MAARAVLDEF TAPAEKAELL EQSRGRIEGL FGVSLAVLGA LGAEEPLPAR IWLQLCGAQE 70 80 90 100 110 120 AVHSAKEYIK GICEPELEER ECYPKDMHCI FVGAESLFLK SLIQDTCADL CILDIGLLGI 130 140 150 160 170 180 RGSAEAVVMA RSHIQQFVKL FENKENLPSS QKESEVKREF KQFVEAHADN YTMDLLILPT 190 200 210 220 230 240 SLKKELLTLT QGEENLFETG DDEVIEMRDS QQTEFTQNAA TGLNISRDET VLQEEARNKA 250 260 270 280 290 300 GTPVSELTKQ MDTVLSSSPD VLFDPINGLT PDEEALSNER ICQKRRFSDS EERHTKKQFS 310 320 330 340 350 360 LENVQEGEIL HDAKTLAGNV IADLSDSSAD SENLSPDIKE TTEEMEYNIL VNFFKTMGYS 370 380 390 400 410 420 QEIVEKVIKV YGPSTEPLLL LEEIEKENKR FQEDREFSAG TVYPETNKTK NKGVYSSTNE 430 440 450 460 470 480 LTTDSTPKKT QAHTQQNMVE KFSQLPFKVE AKPCTSNCRI NTFRTVPIEQ KHEVWGSNQN 490 500 510 520 530 540 YICNTDPETD GLSPSVASPS PKEVNFVSRG ASSHQPRVPL FPENGLHQQP EPLLPNNMKS 550 560 570 580 590 600 ACEKRLGCCS SPHSKPNCST LSPPMPLPQL LPSVTDARSA GPSDHIDSSV TGVQRFRDTL 610 620 630 640 650 660 KIPYKLELKN EPGRTDLKHI VIDGSNVAIT HGLKKFFSCR GIAIAVEYFW KLGNRNITVF 670 680 690 700 710 720 VPQWRTRRDP NVTEQHFLTQ LQELGILSLT PARMVFGERI ASHDDRFLLH LADKTGGIIV 730 740 750 760 770 780 TNDNFREFVN ESVSWREIIT KRLLQYTFVG DIFMVPDDPL GRSGPRLEEF LQKEVCLRDM 790 800 810 820 830 840 QPLLSALPNV GMFDPSFRVP GTQAASTSHQ PPTRIQGAPS SHWLPQQPHF PLLPALPSLQ 850 860 870 880 890 QNLPMPAQRS SAETNELREA LLKIFPDSEQ RLKIDQILVA HPYMKDLNAL SAMVLD

References

[1]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[2]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226 AND SER-258, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-242, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB014515 mRNA. Translation: BAA31590.2. Different initiation. AC023813 Genomic DNA. No translation available. AC026470 Genomic DNA. No translation available. BC108288 mRNA. Translation: AAI08289.1. BC152472 mRNA. Translation: AAI52473.1.
IPI	IPI00005667.
PIR	T00389.
RefSeq	NP_694574.3. NM_153029.3.
UniGene	Hs.511839.
3D structure databases
ProteinModelPortal	O75113.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-222634.
STRING	O75113.
PTM databases
PhosphoSite	O75113.
Proteomic databases
PRIDE	O75113.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262384; ENSP00000262384; ENSG00000102921.
GeneID	9683.
KEGG	hsa:9683.
Organism-specific databases
CTD	9683.
GeneCards	GC16M048572.
H-InvDB	HIX0022055.
HGNC	HGNC:29850. N4BP1.
HPA	HPA040849. HPA041080.
neXtProt	NX_O75113.
PharmGKB	PA162396561.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18019.
GeneTree	ENSGT00550000074448.
HOGENOM	HBG505731.
HOVERGEN	HBG059944.
InParanoid	O75113.
OMA	NYTMDLL.
OrthoDB	EOG4K3KVJ.
PhylomeDB	O75113.
Gene expression databases
ArrayExpress	O75113.
Bgee	O75113.
CleanEx	HS_N4BP1.
Genevestigator	O75113.
GermOnline	ENSG00000102921. Homo sapiens.
Family and domain databases
InterPro	IPR021869. RNase_Zc3h12. [Graphical view]
Pfam	PF11977. RNase_Zc3h12a. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	36365.
PMAP-CutDB	O75113.

Entry information

Entry name

N4BP1_HUMAN

Accession

Primary (citable) accession number: O75113
Secondary accession number(s): A7MD49, Q2YDX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 73 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.