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Protein

NEDD4-binding protein 1

Gene

N4BP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000102921-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD4-binding protein 1
Short name:
N4BP1
Gene namesi
Name:N4BP1
Synonyms:KIAA0615
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:29850. N4BP1.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • NucleusPML body By similarity

  • Note: Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9683.
OpenTargetsiENSG00000102921.
PharmGKBiPA162396561.

Polymorphism and mutation databases

BioMutaiN4BP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966801 – 896NEDD4-binding protein 1Add BLAST896

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphoserineCombined sources1
Modified residuei242PhosphothreonineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei562PhosphoserineBy similarity1

Post-translational modificationi

Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated with SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO75113.
MaxQBiO75113.
PaxDbiO75113.
PeptideAtlasiO75113.
PRIDEiO75113.

PTM databases

iPTMnetiO75113.
PhosphoSitePlusiO75113.

Miscellaneous databases

PMAP-CutDBO75113.

Expressioni

Tissue specificityi

Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.1 Publication

Gene expression databases

BgeeiENSG00000102921.
CleanExiHS_N4BP1.
ExpressionAtlasiO75113. baseline and differential.
GenevisibleiO75113. HS.

Organism-specific databases

HPAiHPA040849.
HPA041080.

Interactioni

Subunit structurei

Interacts with NEDD4. Interacts with ITCH (via WW domain 2) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Azi2Q9QYP62EBI-5278391,EBI-6115874From a different organism.

Protein-protein interaction databases

BioGridi115036. 7 interactors.
DIPiDIP-41205N.
IntActiO75113. 5 interactors.
MINTiMINT-222634.
STRINGi9606.ENSP00000262384.

Structurei

3D structure databases

ProteinModelPortaliO75113.
SMRiO75113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N4BP1 family.Curated

Phylogenomic databases

eggNOGiKOG3777. Eukaryota.
ENOG410ZNK1. LUCA.
GeneTreeiENSGT00750000117218.
HOGENOMiHOG000113716.
HOVERGENiHBG059944.
InParanoidiO75113.
OMAiMARSHIQ.
OrthoDBiEOG091G03B2.
PhylomeDBiO75113.
TreeFamiTF315783.

Family and domain databases

InterProiIPR021869. RNase_Zc3h12_NYN.
[Graphical view]
PfamiPF11977. RNase_Zc3h12a. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O75113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARAVLDEF TAPAEKAELL EQSRGRIEGL FGVSLAVLGA LGAEEPLPAR
60 70 80 90 100
IWLQLCGAQE AVHSAKEYIK GICEPELEER ECYPKDMHCI FVGAESLFLK
110 120 130 140 150
SLIQDTCADL CILDIGLLGI RGSAEAVVMA RSHIQQFVKL FENKENLPSS
160 170 180 190 200
QKESEVKREF KQFVEAHADN YTMDLLILPT SLKKELLTLT QGEENLFETG
210 220 230 240 250
DDEVIEMRDS QQTEFTQNAA TGLNISRDET VLQEEARNKA GTPVSELTKQ
260 270 280 290 300
MDTVLSSSPD VLFDPINGLT PDEEALSNER ICQKRRFSDS EERHTKKQFS
310 320 330 340 350
LENVQEGEIL HDAKTLAGNV IADLSDSSAD SENLSPDIKE TTEEMEYNIL
360 370 380 390 400
VNFFKTMGYS QEIVEKVIKV YGPSTEPLLL LEEIEKENKR FQEDREFSAG
410 420 430 440 450
TVYPETNKTK NKGVYSSTNE LTTDSTPKKT QAHTQQNMVE KFSQLPFKVE
460 470 480 490 500
AKPCTSNCRI NTFRTVPIEQ KHEVWGSNQN YICNTDPETD GLSPSVASPS
510 520 530 540 550
PKEVNFVSRG ASSHQPRVPL FPENGLHQQP EPLLPNNMKS ACEKRLGCCS
560 570 580 590 600
SPHSKPNCST LSPPMPLPQL LPSVTDARSA GPSDHIDSSV TGVQRFRDTL
610 620 630 640 650
KIPYKLELKN EPGRTDLKHI VIDGSNVAIT HGLKKFFSCR GIAIAVEYFW
660 670 680 690 700
KLGNRNITVF VPQWRTRRDP NVTEQHFLTQ LQELGILSLT PARMVFGERI
710 720 730 740 750
ASHDDRFLLH LADKTGGIIV TNDNFREFVN ESVSWREIIT KRLLQYTFVG
760 770 780 790 800
DIFMVPDDPL GRSGPRLEEF LQKEVCLRDM QPLLSALPNV GMFDPSFRVP
810 820 830 840 850
GTQAASTSHQ PPTRIQGAPS SHWLPQQPHF PLLPALPSLQ QNLPMPAQRS
860 870 880 890
SAETNELREA LLKIFPDSEQ RLKIDQILVA HPYMKDLNAL SAMVLD
Length:896
Mass (Da):100,379
Last modified:November 24, 2009 - v4
Checksum:i171EDCC9D3BCF3D3
GO

Sequence cautioni

The sequence BAA31590 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62V → E in BAA31590 (PubMed:9734811).Curated1
Sequence conflicti62V → E in AAI52473 (PubMed:15489334).Curated1
Sequence conflicti600L → P in AAI08289 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014515 mRNA. Translation: BAA31590.2. Different initiation.
AC023813 Genomic DNA. No translation available.
AC026470 Genomic DNA. No translation available.
BC108288 mRNA. Translation: AAI08289.1.
BC152472 mRNA. Translation: AAI52473.1.
CCDSiCCDS45479.1.
PIRiT00389.
RefSeqiNP_694574.3. NM_153029.3.
UniGeneiHs.511839.

Genome annotation databases

EnsembliENST00000262384; ENSP00000262384; ENSG00000102921.
GeneIDi9683.
KEGGihsa:9683.
UCSCiuc002efp.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014515 mRNA. Translation: BAA31590.2. Different initiation.
AC023813 Genomic DNA. No translation available.
AC026470 Genomic DNA. No translation available.
BC108288 mRNA. Translation: AAI08289.1.
BC152472 mRNA. Translation: AAI52473.1.
CCDSiCCDS45479.1.
PIRiT00389.
RefSeqiNP_694574.3. NM_153029.3.
UniGeneiHs.511839.

3D structure databases

ProteinModelPortaliO75113.
SMRiO75113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115036. 7 interactors.
DIPiDIP-41205N.
IntActiO75113. 5 interactors.
MINTiMINT-222634.
STRINGi9606.ENSP00000262384.

PTM databases

iPTMnetiO75113.
PhosphoSitePlusiO75113.

Polymorphism and mutation databases

BioMutaiN4BP1.

Proteomic databases

EPDiO75113.
MaxQBiO75113.
PaxDbiO75113.
PeptideAtlasiO75113.
PRIDEiO75113.

Protocols and materials databases

DNASUi9683.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262384; ENSP00000262384; ENSG00000102921.
GeneIDi9683.
KEGGihsa:9683.
UCSCiuc002efp.3. human.

Organism-specific databases

CTDi9683.
DisGeNETi9683.
GeneCardsiN4BP1.
H-InvDBHIX0022055.
HGNCiHGNC:29850. N4BP1.
HPAiHPA040849.
HPA041080.
neXtProtiNX_O75113.
OpenTargetsiENSG00000102921.
PharmGKBiPA162396561.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3777. Eukaryota.
ENOG410ZNK1. LUCA.
GeneTreeiENSGT00750000117218.
HOGENOMiHOG000113716.
HOVERGENiHBG059944.
InParanoidiO75113.
OMAiMARSHIQ.
OrthoDBiEOG091G03B2.
PhylomeDBiO75113.
TreeFamiTF315783.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000102921-MONOMER.

Miscellaneous databases

ChiTaRSiN4BP1. human.
GenomeRNAii9683.
PMAP-CutDBO75113.
PROiO75113.

Gene expression databases

BgeeiENSG00000102921.
CleanExiHS_N4BP1.
ExpressionAtlasiO75113. baseline and differential.
GenevisibleiO75113. HS.

Family and domain databases

InterProiIPR021869. RNase_Zc3h12_NYN.
[Graphical view]
PfamiPF11977. RNase_Zc3h12a. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiN4BP1_HUMAN
AccessioniPrimary (citable) accession number: O75113
Secondary accession number(s): A7MD49, Q2YDX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: November 24, 2009
Last modified: November 30, 2016
This is version 114 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.